FastQCFastQC Report
Thu 2 Feb 2017
SRR4061951_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061951_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences660252
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAG250.0023523835.1989122
GTATCAA6650.030.4397581
GGATCGT300.00574401929.3324266
GCTAACG300.00574401929.33242620
ATATATC450.001397795224.4436863
CGTAATG450.001397795224.44368626
GGTATCA2400.022.9194281
TAGACGA500.002579673721.999329
CGTGACT500.002579673721.9993214
CTAAACC500.002579673721.999324
GTCCGAC602.8714674E-421.9993212
AACGTAA550.004482297719.9993824
TAACGCC550.004482297719.999384
TCGACAG550.004482297719.9993825
GTATTAT1051.7881139E-618.8594151
CAAGATA708.119681E-418.8565584
ATTATAC708.119681E-418.8565583
CGCAAAA708.119681E-418.85655812
TATCAAC10650.018.7975392
GCGGCAA951.5957477E-518.52574222
Produced by FastQC (version 0.11.5)