##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061951_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	660252
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.329430278136225	32.0	32.0	32.0	32.0	32.0
2	31.505290404269886	32.0	32.0	32.0	32.0	32.0
3	31.593435536734457	32.0	32.0	32.0	32.0	32.0
4	31.665818505661473	32.0	32.0	32.0	32.0	32.0
5	31.643590629032552	32.0	32.0	32.0	32.0	32.0
6	35.289669701871404	36.0	36.0	36.0	36.0	36.0
7	35.30321453020968	36.0	36.0	36.0	36.0	36.0
8	35.25064823733968	36.0	36.0	36.0	36.0	36.0
9	35.33743176847628	36.0	36.0	36.0	36.0	36.0
10	35.228685411024884	36.0	36.0	36.0	36.0	36.0
11	35.326155770826894	36.0	36.0	36.0	36.0	36.0
12	35.27756674724196	36.0	36.0	36.0	36.0	36.0
13	35.28688440171329	36.0	36.0	36.0	36.0	36.0
14	35.25798785918104	36.0	36.0	36.0	36.0	36.0
15	35.23904660644723	36.0	36.0	36.0	36.0	36.0
16	35.252129489952324	36.0	36.0	36.0	36.0	36.0
17	35.242299909731436	36.0	36.0	36.0	36.0	36.0
18	35.2319205394304	36.0	36.0	36.0	36.0	36.0
19	35.2284037004053	36.0	36.0	36.0	36.0	36.0
20	35.22313298558732	36.0	36.0	36.0	36.0	36.0
21	35.22053852165537	36.0	36.0	36.0	36.0	36.0
22	35.19762302878295	36.0	36.0	36.0	36.0	36.0
23	35.180306913118024	36.0	36.0	36.0	36.0	36.0
24	35.16225168572	36.0	36.0	36.0	36.0	36.0
25	35.14943536710226	36.0	36.0	36.0	36.0	36.0
26	35.105948940707485	36.0	36.0	36.0	36.0	36.0
27	35.0947304968406	36.0	36.0	36.0	36.0	36.0
28	35.081614292724595	36.0	36.0	36.0	36.0	36.0
29	35.06396042723082	36.0	36.0	36.0	36.0	36.0
30	35.0509593306798	36.0	36.0	36.0	36.0	36.0
31	35.0448253091244	36.0	36.0	36.0	36.0	36.0
32	35.026383865554365	36.0	36.0	36.0	36.0	36.0
33	35.000773946917235	36.0	36.0	36.0	36.0	36.0
34	34.982400659142264	36.0	36.0	36.0	36.0	36.0
35	34.97793569727922	36.0	36.0	36.0	36.0	36.0
36	34.95245906108577	36.0	36.0	36.0	36.0	36.0
37	34.9500372585013	36.0	36.0	36.0	36.0	36.0
38	34.94359577858151	36.0	36.0	36.0	36.0	36.0
39	34.91940198590841	36.0	36.0	36.0	36.0	36.0
40	34.90318999412346	36.0	36.0	36.0	36.0	36.0
41	34.88460163695074	36.0	36.0	36.0	36.0	36.0
42	34.866222290882874	36.0	36.0	36.0	32.0	36.0
43	34.854167802596585	36.0	36.0	36.0	36.0	36.0
44	34.82038070312547	36.0	36.0	36.0	32.0	36.0
45	34.80136220715727	36.0	36.0	36.0	32.0	36.0
46	34.78206502971592	36.0	36.0	36.0	32.0	36.0
47	34.76142745497174	36.0	36.0	36.0	32.0	36.0
48	34.740970114441154	36.0	36.0	36.0	32.0	36.0
49	34.71732005355531	36.0	36.0	36.0	32.0	36.0
50	34.3356263971938	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	4.0
21	14.0
22	36.0
23	101.0
24	254.0
25	639.0
26	1481.0
27	2755.0
28	4760.0
29	7774.0
30	11536.0
31	17207.0
32	25703.0
33	43448.0
34	99866.0
35	444673.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.254045209381246	18.246924428822496	12.736652324101568	25.762378037694685
2	15.811204614163593	20.623330122321377	37.751201100225984	25.814264163289046
3	18.0888783205175	24.5064377032371	29.13422319693571	28.270460779309687
4	12.789056299715867	16.6874163198294	35.79739251073833	34.72613486971641
5	14.269724695608184	36.826449335177074	33.8993806900709	15.004445279143841
6	33.463536539189704	35.71919727375994	17.179098826202196	13.638167360848163
7	29.767573593112935	30.767949207272377	21.058777557659802	18.40569964195489
8	27.506921599631657	34.07729170074457	19.42349284818524	18.99229385143854
9	27.015896960554453	15.445011904545536	18.781616716041754	38.75747441885826
10	16.209138645757445	27.22146577589432	31.88469233670225	24.68470324164598
11	35.590501808400425	22.37433585964147	22.72389935963844	19.31126297231966
12	23.93510356651703	24.415677650351682	29.271399405075638	22.37781937805565
13	28.946977820589716	20.50504958712734	25.587957325384853	24.960015266898093
14	23.432568170940794	19.698387888260847	25.919951775988565	30.949092164809798
15	24.908065405330085	27.40665685223218	22.577440128920472	25.107837613517265
16	24.77326701481867	26.59318922586664	24.093976808714302	24.53956695060038
17	23.597959566953225	26.847476418094907	25.543731787256984	24.01083222769488
18	23.696139801378564	26.705298439532843	26.01768115459121	23.58088060449738
19	25.016357390814413	25.93100816052053	25.915862428284957	23.136772020380096
20	25.533207118515715	25.305566073457026	25.36978417266187	23.791442635365392
21	25.318363291591695	25.647480053070648	25.33411485311669	23.70004180222097
22	25.401513361564977	25.076788862434345	25.602345771008643	23.919352004992035
23	24.731920539430398	25.530403542889683	25.583262148391828	24.15441376928809
24	24.485116153676053	25.602426974188408	25.56395646471164	24.348500407423902
25	24.81344216063285	25.02003026121884	25.8968180282953	24.26970954985301
26	24.19852450053086	25.986026526275612	25.509692526024192	24.305756447169333
27	24.41378265808406	26.014085573646344	25.326012873911395	24.246118894358197
28	24.675043393180076	25.56592885638902	25.646505372272593	24.11252237815831
29	24.268759617598235	25.904508608886356	25.631883777005005	24.1948479965104
30	24.520631766437862	25.89239735612895	25.436353832825443	24.15061704460775
31	24.803104288085688	25.741388084477347	25.14615720153639	24.30935042590057
32	24.71351760696706	25.61756910261265	25.38099204846649	24.287921241953807
33	24.219425642672256	25.832902941457114	25.41972674028513	24.5279446755855
34	24.474439907428724	25.520410512413516	25.929044843755676	24.07610473640208
35	24.526090038348993	25.506473285957483	25.823170547003265	24.14426612869026
36	24.453996352907676	25.775461490461215	25.794696570400394	23.97584558623071
37	24.657019698837697	25.446427219110568	25.71920598810739	24.17734709394434
38	24.500342293548524	25.520407359614204	25.80620732689943	24.173043019937843
39	24.690645607849195	25.87671867758381	25.345553021146667	24.08708269342033
40	24.83937902309731	25.566527830367285	25.424914804998107	24.169178341537297
41	24.530099387198103	25.79962619993154	25.530181174895418	24.140093237974934
42	24.527844632901	25.774404476104067	25.48465125860134	24.213099632393593
43	24.46726171993923	25.352419871527864	25.53311694114547	24.647201467387436
44	24.2355991839121	25.57272315566104	25.744786231318812	24.446891429108046
45	24.51097254176139	25.734014500809554	25.424581098662756	24.330431858766296
46	24.46701199563795	25.27883799830365	25.727614200896642	24.52653580516176
47	24.43309180792672	25.750929953593193	25.68837770050042	24.127600537979667
48	24.165371671255947	26.026378526401245	25.343135918477387	24.465113883865417
49	24.573403652688626	25.525198947027228	25.474307282657755	24.42709011762639
50	24.356978198902826	25.729808161249967	25.51079755604763	24.402416083799576
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3.0
1	7.5
2	12.0
3	38.5
4	65.0
5	77.5
6	90.0
7	76.5
8	63.0
9	80.5
10	98.0
11	185.5
12	273.0
13	448.0
14	623.0
15	775.0
16	927.0
17	979.5
18	1032.0
19	1114.5
20	1197.0
21	1509.0
22	1821.0
23	2328.5
24	2836.0
25	3579.0
26	4322.0
27	5454.5
28	6587.0
29	8002.0
30	9417.0
31	11210.0
32	13003.0
33	15343.5
34	17684.0
35	20810.0
36	23936.0
37	25677.0
38	27418.0
39	30998.0
40	34578.0
41	37279.5
42	39981.0
43	41162.5
44	42344.0
45	43980.5
46	45617.0
47	47089.5
48	48562.0
49	49279.5
50	49997.0
51	49522.5
52	49048.0
53	48444.5
54	47841.0
55	45949.0
56	44057.0
57	41360.0
58	38663.0
59	35085.0
60	31507.0
61	27739.5
62	23972.0
63	20505.5
64	17039.0
65	14768.5
66	12498.0
67	10391.0
68	8284.0
69	6989.5
70	5695.0
71	4592.0
72	3489.0
73	2877.0
74	2265.0
75	1854.0
76	1443.0
77	1186.5
78	930.0
79	733.5
80	537.0
81	408.0
82	279.0
83	178.0
84	77.0
85	68.0
86	59.0
87	39.5
88	20.0
89	31.5
90	43.0
91	25.0
92	7.0
93	4.0
94	1.0
95	3.5
96	6.0
97	5.5
98	5.0
99	3.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0321089523394098
2	0.0036349757365369585
3	1.514573223557066E-4
4	0.0
5	1.514573223557066E-4
6	3.029146447114132E-4
7	0.0
8	0.0
9	0.0
10	1.514573223557066E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.058292894228264E-4
17	0.0
18	1.514573223557066E-4
19	0.0
20	3.029146447114132E-4
21	0.0
22	0.0
23	0.0
24	9.087439341342396E-4
25	1.514573223557066E-4
26	1.514573223557066E-4
27	3.029146447114132E-4
28	0.001514573223557066
29	6.058292894228264E-4
30	0.0012116585788456528
31	6.058292894228264E-4
32	3.029146447114132E-4
33	1.514573223557066E-4
34	6.058292894228264E-4
35	0.0
36	0.0
37	0.001514573223557066
38	0.0
39	9.087439341342396E-4
40	3.029146447114132E-4
41	9.087439341342396E-4
42	0.005301006282449732
43	0.004695176993026904
44	0.004392262348315492
45	0.001968945190624186
46	0.0018174878682684793
47	6.058292894228264E-4
48	0.002726231802402719
49	0.003937890381248372
50	0.001514573223557066
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	660252.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.72148562519714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	53.96470215081012	17.65805225695778
2	15.793470535027229	10.33571638167736
3	8.272056536047298	8.120219371052691
4	5.386785009957281	7.050544330773784
5	3.56003312995563	5.8244786443534355
6	2.564044423867986	5.033960565477791
7	1.9078572542938443	4.369954660491287
8	1.4407150624965193	3.771386976598788
9	1.1690276740241345	3.442709000793452
>10	5.814536380752143	31.309509969363162
>50	0.11237650548288414	2.410492976391034
>100	0.01439533728497186	0.672974866069508
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	1.514573223557066E-4	0.0
16	0.0	0.0	0.0	4.543719670671198E-4	0.0
17	0.0	0.0	0.0	4.543719670671198E-4	0.0
18	0.0	0.0	0.0	7.57286611778533E-4	0.0
19	0.0	0.0	0.0	7.57286611778533E-4	0.0
20	0.0	0.0	0.0	0.0010602012564899462	0.0
21	0.0	0.0	0.0	0.0012116585788456528	0.0
22	0.0	0.0	0.0	0.0012116585788456528	0.0
23	0.0	0.0	0.0	0.0013631159012013594	0.0
24	0.0	0.0	0.0	0.0016660305459127727	0.0
25	0.0	0.0	0.0	0.0021204025129798925	0.0
26	0.0	0.0	0.0	0.002726231802402719	0.0
27	0.0	0.0	0.0	0.003029146447114132	0.0
28	0.0	0.0	0.0	0.0033320610918255453	0.0
29	0.0	0.0	0.0	0.004846634315382611	0.0
30	0.0	0.0	0.0	0.0065126648612953844	0.0
31	0.0	0.0	0.0	0.009541811308409516	0.0
32	0.0	0.0	0.0	0.012570957755523648	0.0
33	0.0	0.0	0.0	0.017266134748550554	0.0
34	0.0	0.0	0.0	0.02180985441922175	0.0
35	0.0	0.0	0.0	0.027110860701671482	0.0
36	0.0	0.0	0.0	0.033169153595899746	0.0
37	0.0	0.0	0.0	0.04331679419373209	0.0
38	0.0	0.0	0.0	0.05770523981752422	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATAG	25	0.00235238	35.19891	22
GTATCAA	665	0.0	30.439758	1
GGATCGT	30	0.005744019	29.332426	6
GCTAACG	30	0.005744019	29.332426	20
ATATATC	45	0.0013977952	24.443686	3
CGTAATG	45	0.0013977952	24.443686	26
GGTATCA	240	0.0	22.919428	1
TAGACGA	50	0.0025796737	21.99932	9
CGTGACT	50	0.0025796737	21.99932	14
CTAAACC	50	0.0025796737	21.99932	4
GTCCGAC	60	2.8714674E-4	21.99932	12
AACGTAA	55	0.0044822977	19.99938	24
TAACGCC	55	0.0044822977	19.99938	4
TCGACAG	55	0.0044822977	19.99938	25
GTATTAT	105	1.7881139E-6	18.859415	1
CAAGATA	70	8.119681E-4	18.856558	4
ATTATAC	70	8.119681E-4	18.856558	3
CGCAAAA	70	8.119681E-4	18.856558	12
TATCAAC	1065	0.0	18.797539	2
GCGGCAA	95	1.5957477E-5	18.525742	22
>>END_MODULE