FastQCFastQC Report
Thu 2 Feb 2017
SRR4061950_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061950_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781515
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG93891.2013844903808628No Hit
TCCATGTACTCTGCGTTGATACCAC80901.035168870719052No Hit
GTCCTAAAGTGTGTATTTCTCATTT42550.5444553207551999No Hit
GTACATGGGAAGCAGTGGTATCAAC42090.5385693172875761No Hit
CCCATGTACTCTGCGTTGATACCAC41320.5287166593091623No Hit
CTGTAGGACGTGGAATATGGCAAGA40580.5192478711221153No Hit
GTCCTACAGTGGACATTTCTAAATT40410.517072608971037No Hit
CTTTAGGACGTGAAATATGGCGAGG38060.48700280864730683No Hit
GAGTACATGGAAGCAGTGGTATCAA36410.4658899701221346No Hit
CATGTACTCTGCGTTGATACCACTG31290.40037619239553945No Hit
GTCCTACAGTGTGCATTTCTCATTT26710.3417720709135461No Hit
GCTTCCATGTACTCTGCGTTGATAC26550.33972476535959006No Hit
CATGGAAGCAGTGGTATCAACGCAG22700.2904614754675214No Hit
GCGTTGATACCACTGCTTCCATGTA21330.2729314216617723No Hit
CTGTAGGACCTGGAATATGGCGAGA20470.2619271543092583No Hit
GTATCAACGCAGAGTACATGGGAAG19100.2443971005035092No Hit
CTGAAGGACCTGGAATATGGCGAGA18290.23403261613660647No Hit
ATTTAGAAATGTCCACTGTAGGACG16830.21535095295675707No Hit
GTATCAACGCAGAGTACATGGAAGC16030.2051144251869766No Hit
GTCCTTCAGTGTGCATTTCTCATTT15310.19590155019417413No Hit
GTATCAACGCAGAGTACTTTTTTTT15150.19385424464021803No Hit
TTTCTAAATTTTCCACCTTTTTCAG15070.19283059186324No Hit
CAGTGGTATCAACGCAGAGTACATG15000.1919348956833842No Hit
ACTCTGCGTTGATACCACTGCTTCC14670.18771232797834975No Hit
GAGTACATGGGAAGCAGTGGTATCA12890.16493605369058817No Hit
GAATATGGCAAGAAAACTGAAAATC12590.16109735577692047No Hit
GGTATCAACGCAGAGTACATGGGAA12550.16058552938843146No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12010.1536758731438296No Hit
ACGCAGAGTACATGGAAGCAGTGGT11400.14587052071937198No Hit
GGAATATGGCGAGAAAACTGAAAAT11250.14395117176253816No Hit
TATCAACGCAGAGTACATGGGAAGC11220.14356730197117137No Hit
GCTTCCCATGTACTCTGCGTTGATA10560.13512216656110249No Hit
TATCAACGCAGAGTACTTTTTTTTT10460.13384260058987993No Hit
TATCAACGCAGAGTACATGGAAGCA10320.13205120823016833No Hit
GAAATATGGCGAGGAAAACTGAAAA10030.12834046691362289No Hit
GCGTTGATACCACTGCTTCCCATGT9960.12744477073376712No Hit
ATTCCAGGTCCTTCAGTGTGCATTT9480.12130285407189881No Hit
GCCATATTCCACGTCCTACAGTGGA9150.11708028636686436No Hit
ACGCAGAGTACATGGGAAGCAGTGG9140.1169523297697421No Hit
GGTATCAACGCAGAGTACATGGAAG9090.11631254678413083No Hit
CTGTAGGACATGGAATATGGCAAGA9010.11528889400715277No Hit
AAAAAGTACTCTGCGTTGATACCAC8950.11452115442441924No Hit
GTCCACTGTAGGACGTGGAATATGG8470.10837923776255096No Hit
GTACTCTGCGTTGATACCACTGCTT8410.1076114981798174No Hit
CCATATTTCACGTCCTAAAGTGTGT8270.10582010582010583No Hit
ACAGTGGACATTTCTAAATTTTCCA8250.10556419262586132No Hit
GTGGTATCAACGCAGAGTACATGGA8030.10274914748917169No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTAT307.7113626E-419.0024368
TAGCGTA250.00602498219.0024367
GTATAGT400.00529171314.2436081
TAGAAAT5250.013.7514994
GTATTAG903.6508936E-813.7160681
TAGGACC9250.013.6585834
GTATTAA707.2851453E-613.5653421
AATCTGC500.001496933313.3017046
ACCTCCG500.001499104513.29914719
GTTATAT751.4831752E-512.6609851
AAATGTC5350.012.6090937
ATGTCCA5200.012.6073859
ATAGGAC1750.012.4833143
AATGTCC5400.012.3163948
GAAATGT5700.012.1682266
CGAGCCG550.003066090912.09090915
TGTCCAC5550.011.812325510
TATAGGA1701.8189894E-1211.7330382
GACAGTT658.006947E-411.6938067
AGGACCT15450.011.6843455
Produced by FastQC (version 0.11.5)