##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061950_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	781515
Sequences flagged as poor quality	0
Sequence length	25
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.278648522421193	32.0	32.0	32.0	32.0	32.0
2	31.01156343768194	32.0	32.0	32.0	32.0	32.0
3	31.021134591146684	32.0	32.0	32.0	32.0	32.0
4	30.999984645208347	32.0	32.0	32.0	32.0	32.0
5	31.015916521116036	32.0	32.0	32.0	32.0	32.0
6	34.669920602931484	36.0	36.0	36.0	32.0	36.0
7	34.59623807604461	36.0	36.0	36.0	32.0	36.0
8	34.55985361765289	36.0	36.0	36.0	32.0	36.0
9	34.59079224327108	36.0	36.0	36.0	32.0	36.0
10	34.40683160272036	36.0	36.0	36.0	32.0	36.0
11	34.58411674759921	36.0	36.0	36.0	32.0	36.0
12	34.45639047235178	36.0	36.0	36.0	32.0	36.0
13	34.44663122268926	36.0	36.0	36.0	32.0	36.0
14	34.410956923411575	36.0	36.0	36.0	32.0	36.0
15	34.363193284837784	36.0	36.0	36.0	32.0	36.0
16	34.33860130643686	36.0	36.0	36.0	32.0	36.0
17	34.28995220821098	36.0	36.0	36.0	32.0	36.0
18	34.24470547590257	36.0	36.0	36.0	32.0	36.0
19	34.213608184103954	36.0	36.0	36.0	32.0	36.0
20	34.15440778487937	36.0	36.0	36.0	32.0	36.0
21	34.067658330294364	36.0	36.0	36.0	32.0	36.0
22	33.96192907365822	36.0	36.0	36.0	32.0	36.0
23	33.89220168518838	36.0	36.0	36.0	32.0	36.0
24	33.82325995022488	36.0	36.0	36.0	32.0	36.0
25	33.35851263251505	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	7.0
5	75.0
6	204.0
7	21.0
8	143.0
9	109.0
10	69.0
11	12.0
12	30.0
13	33.0
14	187.0
15	249.0
16	360.0
17	510.0
18	639.0
19	845.0
20	1200.0
21	1573.0
22	2245.0
23	3104.0
24	4205.0
25	5644.0
26	7627.0
27	9805.0
28	13182.0
29	18431.0
30	24622.0
31	34277.0
32	48880.0
33	69581.0
34	155144.0
35	378498.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.34566849134875	18.295572906669943	12.848653753138173	25.510104848843127
2	12.826497252132077	20.31925170982003	42.7579764943731	24.096274543674788
3	19.34900635050217	26.278257687332136	28.344634062389236	26.028101899776455
4	12.111540528780937	17.93798469453047	36.1421514678406	33.808323308847996
5	12.169098822324628	38.767537122375835	34.98489503328213	14.078469022017408
6	31.887058690964594	35.093447652412635	18.937170602787294	14.082323053835477
7	27.942395318658882	31.095997981120398	22.23470500146676	18.726901698753956
8	25.7403939031358	38.27616775856459	19.8590055316565	16.124432806643114
9	26.876712785079825	16.246715360263	19.544359131714852	37.33221272294232
10	16.89679562088689	28.294713697029337	32.76172683846895	22.04676384361482
11	33.24871437706395	21.117149626903444	25.354163009171398	20.279972986861207
12	25.99916712047923	23.731043982445463	30.966076176442325	19.303712720632987
13	31.426147924536036	22.17484227088918	24.65276532060908	21.7462444839657
14	21.746215785124495	23.69382931157076	24.90055025655151	29.65940464675323
15	23.377087663277884	33.296515457101194	23.242928789443607	20.083468090177313
16	21.568524452382203	25.99699942474027	31.48733467688724	20.947141445990287
17	21.494491302292268	29.15413719277431	29.38258254718492	19.9687889577485
18	22.769712467267595	23.98715301086895	34.2182399573646	19.024894564498855
19	24.637150231524473	27.499855897629676	25.929844561576544	21.933149309269304
20	24.582506116070856	26.0735465525854	29.92545438243695	19.418492948906792
21	22.012608421667426	27.085692182368703	24.655330529184834	26.246368866779036
22	23.471143192022186	31.096180820198168	24.61286211114883	20.81981387663082
23	19.424430026664073	30.862317597006246	27.155729957762674	22.557522418567004
24	23.282172403325568	28.6265960839666	27.713743005024728	20.377488507683104
25	22.859572702227425	27.859015557778438	27.095791702262005	22.18562003773213
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	417.0
1	417.0
2	496.5
3	576.0
4	576.0
5	576.0
6	1305.5
7	2035.0
8	2035.0
9	2035.0
10	1838.5
11	1642.0
12	1642.0
13	1642.0
14	1914.5
15	2187.0
16	2187.0
17	2187.0
18	3793.0
19	5399.0
20	5399.0
21	5399.0
22	10584.0
23	15769.0
24	15769.0
25	15769.0
26	26469.0
27	37169.0
28	37169.0
29	37169.0
30	49944.5
31	62720.0
32	62720.0
33	62720.0
34	67627.5
35	72535.0
36	72535.0
37	72535.0
38	75826.5
39	79118.0
40	79118.0
41	79118.0
42	87326.0
43	95534.0
44	95534.0
45	95534.0
46	119714.5
47	143895.0
48	143895.0
49	143895.0
50	119187.5
51	94480.0
52	94480.0
53	94480.0
54	75814.5
55	57149.0
56	57149.0
57	57149.0
58	49716.5
59	42284.0
60	42284.0
61	42284.0
62	36549.0
63	30814.0
64	30814.0
65	30814.0
66	24860.0
67	18906.0
68	18906.0
69	18906.0
70	14183.0
71	9460.0
72	9460.0
73	9460.0
74	7134.0
75	4808.0
76	4808.0
77	4808.0
78	3673.5
79	2539.0
80	2539.0
81	2539.0
82	1805.0
83	1071.0
84	1071.0
85	1071.0
86	766.0
87	461.0
88	461.0
89	461.0
90	327.5
91	194.0
92	194.0
93	194.0
94	122.0
95	50.0
96	50.0
97	50.0
98	176.5
99	303.0
100	303.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011516093741003053
2	0.0
3	2.5591319424451227E-4
4	0.012155876726614332
5	0.04030632809351068
6	0.06948043223738508
7	0.11311363185607443
8	0.11733619956110887
9	0.12642111795678906
10	0.1385769946834034
11	0.14766191307908358
12	0.1394726908632592
13	0.13601786274095828
14	0.12411789920858844
15	0.14036838704311497
16	0.12770068392801162
17	0.13077164225894575
18	0.12066307108628753
19	0.10492440964025003
20	0.10044592874097107
21	0.09814270999277046
22	0.1087631075539177
23	0.08816209541723447
24	0.09929431936687076
25	0.09571153464744758
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	781515.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.76713532826468
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.07877051126593	36.51520426764895
2	13.005872423900417	12.164947914213931
3	3.904516652400441	5.478091760228232
4	1.580023944439106	2.955727745259289
5	0.8128404398424464	1.900710942519894
6	0.507247855394722	1.4233517458930196
7	0.3344650421034871	1.0949380320639266
8	0.23801147296444572	0.8904891812646275
9	0.18701746263875643	0.7871643885577866
>10	1.1298169809909915	10.008733013281084
>50	0.11455460213315392	3.8055453306046996
>100	0.0868087027471854	8.179843240803555
>500	0.010439021216417762	3.541014298997157
>1k	0.0090654657932049	9.008632270723483
>5k	5.494221692851454E-4	2.2456058679403275
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	9389	1.2013844903808628	No Hit
TCCATGTACTCTGCGTTGATACCAC	8090	1.035168870719052	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	4255	0.5444553207551999	No Hit
GTACATGGGAAGCAGTGGTATCAAC	4209	0.5385693172875761	No Hit
CCCATGTACTCTGCGTTGATACCAC	4132	0.5287166593091623	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4058	0.5192478711221153	No Hit
GTCCTACAGTGGACATTTCTAAATT	4041	0.517072608971037	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3806	0.48700280864730683	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3641	0.4658899701221346	No Hit
CATGTACTCTGCGTTGATACCACTG	3129	0.40037619239553945	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2671	0.3417720709135461	No Hit
GCTTCCATGTACTCTGCGTTGATAC	2655	0.33972476535959006	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2270	0.2904614754675214	No Hit
GCGTTGATACCACTGCTTCCATGTA	2133	0.2729314216617723	No Hit
CTGTAGGACCTGGAATATGGCGAGA	2047	0.2619271543092583	No Hit
GTATCAACGCAGAGTACATGGGAAG	1910	0.2443971005035092	No Hit
CTGAAGGACCTGGAATATGGCGAGA	1829	0.23403261613660647	No Hit
ATTTAGAAATGTCCACTGTAGGACG	1683	0.21535095295675707	No Hit
GTATCAACGCAGAGTACATGGAAGC	1603	0.2051144251869766	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	1531	0.19590155019417413	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1515	0.19385424464021803	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	1507	0.19283059186324	No Hit
CAGTGGTATCAACGCAGAGTACATG	1500	0.1919348956833842	No Hit
ACTCTGCGTTGATACCACTGCTTCC	1467	0.18771232797834975	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1289	0.16493605369058817	No Hit
GAATATGGCAAGAAAACTGAAAATC	1259	0.16109735577692047	No Hit
GGTATCAACGCAGAGTACATGGGAA	1255	0.16058552938843146	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1201	0.1536758731438296	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1140	0.14587052071937198	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1125	0.14395117176253816	No Hit
TATCAACGCAGAGTACATGGGAAGC	1122	0.14356730197117137	No Hit
GCTTCCCATGTACTCTGCGTTGATA	1056	0.13512216656110249	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1046	0.13384260058987993	No Hit
TATCAACGCAGAGTACATGGAAGCA	1032	0.13205120823016833	No Hit
GAAATATGGCGAGGAAAACTGAAAA	1003	0.12834046691362289	No Hit
GCGTTGATACCACTGCTTCCCATGT	996	0.12744477073376712	No Hit
ATTCCAGGTCCTTCAGTGTGCATTT	948	0.12130285407189881	No Hit
GCCATATTCCACGTCCTACAGTGGA	915	0.11708028636686436	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	914	0.1169523297697421	No Hit
GGTATCAACGCAGAGTACATGGAAG	909	0.11631254678413083	No Hit
CTGTAGGACATGGAATATGGCAAGA	901	0.11528889400715277	No Hit
AAAAAGTACTCTGCGTTGATACCAC	895	0.11452115442441924	No Hit
GTCCACTGTAGGACGTGGAATATGG	847	0.10837923776255096	No Hit
GTACTCTGCGTTGATACCACTGCTT	841	0.1076114981798174	No Hit
CCATATTTCACGTCCTAAAGTGTGT	827	0.10582010582010583	No Hit
ACAGTGGACATTTCTAAATTTTCCA	825	0.10556419262586132	No Hit
GTGGTATCAACGCAGAGTACATGGA	803	0.10274914748917169	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	1.2795659712225613E-4	0.0
9	0.0	0.0	0.0	1.2795659712225613E-4	0.0
10	0.0	0.0	0.0	1.2795659712225613E-4	0.0
11	0.0	0.0	0.0	1.2795659712225613E-4	0.0
12	0.0	0.0	0.0	1.2795659712225613E-4	1.2795659712225613E-4
13	0.0	0.0	0.0	2.5591319424451227E-4	1.2795659712225613E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTAT	30	7.7113626E-4	19.002436	8
TAGCGTA	25	0.006024982	19.002436	7
GTATAGT	40	0.005291713	14.243608	1
TAGAAAT	525	0.0	13.751499	4
GTATTAG	90	3.6508936E-8	13.716068	1
TAGGACC	925	0.0	13.658583	4
GTATTAA	70	7.2851453E-6	13.565342	1
AATCTGC	50	0.0014969333	13.301704	6
ACCTCCG	50	0.0014991045	13.299147	19
GTTATAT	75	1.4831752E-5	12.660985	1
AAATGTC	535	0.0	12.609093	7
ATGTCCA	520	0.0	12.607385	9
ATAGGAC	175	0.0	12.483314	3
AATGTCC	540	0.0	12.316394	8
GAAATGT	570	0.0	12.168226	6
CGAGCCG	55	0.0030660909	12.090909	15
TGTCCAC	555	0.0	11.8123255	10
TATAGGA	170	1.8189894E-12	11.733038	2
GACAGTT	65	8.006947E-4	11.693806	7
AGGACCT	1545	0.0	11.684345	5
>>END_MODULE