FastQCFastQC Report
Thu 2 Feb 2017
SRR4061950_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061950_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences781515
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21030.26909272374810467No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA19620.25105084355386653No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA19420.2484917116114214No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA16840.21547890955387933No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC16520.21138429844596712No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA15320.19602950679129638No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12410.15879413702871986No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA9590.12271037664024363No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA9030.11554480720139729No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT8720.11157815269060734No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG8240.10543623602873906No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA7820.1000620589496043No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAA250.002352101335.2005620
CGCGAAA250.002352101335.2005621
GCGATTA300.00574154329.33567842
GGCGCGA300.00574333929.33380119
TCGCGAG300.00574333929.33380118
TATCGCG300.005745135229.33192416
ATCGCGA300.005745135229.33192417
TAGGACC10450.025.4724624
AATAGCG450.001398162924.4432725
ACGCTAT852.351535E-723.29300110
CTATACT952.683555E-823.1567844
TGTCGAG1254.0017767E-1122.88036718
TATGTCG1254.0017767E-1122.87890216
CGAATGC703.2099077E-522.00316843
TCGAGGA1105.2459654E-922.00035320
TACGCTA904.079866E-721.9989439
CGATGGA500.002580348421.99894331
ACCGTCG500.002580348421.9989438
ATTACCG956.8630834E-720.84110527
AGGACCT18650.020.5244855
Produced by FastQC (version 0.11.5)