##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061950_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	781515
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.118385443657512	32.0	32.0	32.0	32.0	32.0
2	31.509583309341473	32.0	32.0	32.0	32.0	32.0
3	31.624932342949272	32.0	32.0	32.0	32.0	32.0
4	31.70955004062622	32.0	32.0	32.0	32.0	32.0
5	31.70011068245651	32.0	32.0	32.0	32.0	32.0
6	35.36833458091016	36.0	36.0	36.0	36.0	36.0
7	35.3691509439998	36.0	36.0	36.0	36.0	36.0
8	35.34194481231966	36.0	36.0	36.0	36.0	36.0
9	35.38116350933763	36.0	36.0	36.0	36.0	36.0
10	35.29631293065392	36.0	36.0	36.0	36.0	36.0
11	35.37624869644217	36.0	36.0	36.0	36.0	36.0
12	35.3131302662137	36.0	36.0	36.0	36.0	36.0
13	35.293724368694136	36.0	36.0	36.0	36.0	36.0
14	35.270606450292064	36.0	36.0	36.0	36.0	36.0
15	35.235840642853944	36.0	36.0	36.0	36.0	36.0
16	35.21342264703812	36.0	36.0	36.0	36.0	36.0
17	35.22319213322841	36.0	36.0	36.0	36.0	36.0
18	35.18098948836555	36.0	36.0	36.0	36.0	36.0
19	35.14284946546132	36.0	36.0	36.0	36.0	36.0
20	35.12060293148564	36.0	36.0	36.0	36.0	36.0
21	35.03968317946553	36.0	36.0	36.0	36.0	36.0
22	34.94856528665477	36.0	36.0	36.0	36.0	36.0
23	34.89660978995924	36.0	36.0	36.0	36.0	36.0
24	34.83842280698387	36.0	36.0	36.0	32.0	36.0
25	34.737489363607864	36.0	36.0	36.0	32.0	36.0
26	34.6723300256553	36.0	36.0	36.0	32.0	36.0
27	34.51892030223348	36.0	36.0	36.0	32.0	36.0
28	34.39419460918856	36.0	36.0	36.0	32.0	36.0
29	34.339468852165346	36.0	36.0	36.0	32.0	36.0
30	34.248902452288185	36.0	36.0	36.0	32.0	36.0
31	34.2186036096556	36.0	36.0	36.0	32.0	36.0
32	34.163943110496916	36.0	36.0	36.0	32.0	36.0
33	34.16499107502735	36.0	36.0	36.0	32.0	36.0
34	34.10676442550687	36.0	36.0	36.0	32.0	36.0
35	33.98534129223368	36.0	36.0	36.0	32.0	36.0
36	33.85597077471322	36.0	36.0	36.0	32.0	36.0
37	33.64562164513797	36.0	36.0	36.0	27.0	36.0
38	33.57469274422116	36.0	36.0	36.0	21.0	36.0
39	33.16794815198685	36.0	36.0	36.0	14.0	36.0
40	33.15059339871915	36.0	36.0	36.0	14.0	36.0
41	33.16800701202152	36.0	36.0	36.0	14.0	36.0
42	32.951059160732676	36.0	36.0	36.0	14.0	36.0
43	33.04941427867667	36.0	36.0	36.0	14.0	36.0
44	32.9804136836785	36.0	36.0	36.0	14.0	36.0
45	32.71988637454176	36.0	36.0	36.0	14.0	36.0
46	32.759218952931164	36.0	36.0	36.0	14.0	36.0
47	32.65338989014926	36.0	36.0	36.0	14.0	36.0
48	32.71278606296744	36.0	36.0	36.0	14.0	36.0
49	32.71094092883694	36.0	36.0	36.0	14.0	36.0
50	32.36088622739167	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	5.0
19	6.0
20	22.0
21	54.0
22	178.0
23	482.0
24	1333.0
25	3401.0
26	7218.0
27	12993.0
28	18625.0
29	22887.0
30	28397.0
31	35840.0
32	48118.0
33	60254.0
34	121772.0
35	419930.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.84811199158297	18.978129283873884	13.309432170316907	25.864326554226242
2	12.388193774424622	20.842727828777484	42.355717184952766	24.413361211845125
3	19.046235895966035	26.307910031042308	28.74778954695629	25.898064526035363
4	12.010773945477695	17.94284178806549	36.35989072506606	33.68649354139076
5	11.961894525376993	39.017421290698195	34.85141040159178	14.169273782333033
6	32.267025628492426	35.02104251624733	18.657527130066935	14.054404725193312
7	27.959412167392824	31.009129703204675	22.271869381905656	18.75958874749685
8	26.000140752256833	38.18672706218051	19.92117873617269	15.891953449389968
9	26.929361560558657	15.871480393850407	19.593481890942595	37.60567615464834
10	16.957747343934138	28.374064155042845	32.882882306500335	21.785306194522676
11	33.456683492959186	20.712846202568088	25.651842894890052	20.17862740958267
12	25.97416556304102	23.619764175991502	31.08628753126939	19.319782729698087
13	31.776741329341085	21.835537385718766	24.73234678796952	21.655374496970627
14	21.7661849100785	23.349775756063543	25.257096792767893	29.626942541090063
15	23.411450835876472	33.28343026045565	23.239221256149914	20.06589764751796
16	21.86550309978823	25.719093287950813	31.41541001017268	20.999993602088278
17	21.635157354625314	29.025802447809703	29.33648106562254	20.002559131942448
18	22.881641124331907	23.77490841483123	34.414014866035494	18.929435594801365
19	25.01820182594064	27.02648061777445	26.12054791014888	21.83476964613603
20	24.78832725751203	25.702067655944305	30.1297611172175	19.379843969326167
21	22.189465333358925	26.72475896176017	24.87284313160976	26.212932573271146
22	23.61669321766057	30.67836189964364	24.784297166401156	20.92064771629463
23	19.74459863214398	30.060971318528757	27.483797495889394	22.710632553437872
24	23.669269757020732	28.05489906040509	27.802182194145413	20.473648988428767
25	23.284261978336946	27.15047056038592	27.234793957889487	22.33047350338765
26	19.27934383437192	28.574682345715345	29.275888984146075	22.870084835766658
27	22.338152234275874	29.417304537649102	27.21834509268003	21.026198135394996
28	20.953402192717558	27.838658163317536	26.49316183320196	24.714777810762946
29	22.266637662985758	27.440083940064746	27.185448682678405	23.10782971427109
30	22.21652089904606	26.366003621264372	29.109847151933156	22.307628327756415
31	23.858202528697685	25.28428975226133	27.414015705513307	23.443492013527674
32	20.72900458602899	28.925891614118417	28.265882196380314	22.07922160347228
33	19.41377739561261	29.502298109306064	29.999155483217148	21.084769011864182
34	20.179014715291107	29.35278310940499	30.236980166346765	20.231222008957133
35	21.19967447716734	29.436016337561032	28.90051592298007	20.463793262291556
36	20.45542661696794	28.595320079487248	29.999014726637824	20.950238576906983
37	21.729077201046206	28.62025458993822	26.487155338762264	23.16351287025331
38	22.192920161481226	30.652898536816313	25.360485723242675	21.793695578459786
39	23.620510733803965	27.24826392221143	27.139243227473216	21.991982116511387
40	24.878345265286015	27.128973852069375	27.674836695392923	20.317844187251684
41	23.369665359771517	26.066800085988923	29.0307308034846	21.53280375075496
42	23.22902457189217	29.59324957579511	28.329592481784143	18.848133370528576
43	21.015926313607363	29.03564290046758	27.97802614034504	21.970404645580018
44	20.47487123708372	29.146549793659425	28.995553280655173	21.383025688601684
45	21.498602665683926	29.585611039452942	27.33516061666974	21.580625678193396
46	21.19029795837412	26.80473713506977	29.331976941336045	22.672987965220063
47	25.032085527283893	26.135213466324593	28.282480598332704	20.55022040805881
48	20.406259477319054	30.568632745169044	26.190095345828574	22.835012431683325
49	22.218511352701515	27.19837028847532	28.91791044776119	21.665207911061973
50	20.0837885529072	32.88112083184579	24.9917145558938	22.04337605935321
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	221.0
1	154.5
2	88.0
3	329.0
4	570.0
5	488.0
6	406.0
7	334.0
8	262.0
9	357.0
10	452.0
11	619.0
12	786.0
13	1235.0
14	1684.0
15	1948.0
16	2212.0
17	2290.5
18	2369.0
19	2307.0
20	2245.0
21	2536.5
22	2828.0
23	3178.5
24	3529.0
25	4616.5
26	5704.0
27	8373.0
28	11042.0
29	15457.5
30	19873.0
31	25476.0
32	31079.0
33	40285.5
34	49492.0
35	58038.5
36	66585.0
37	65663.5
38	64742.0
39	59139.5
40	53537.0
41	49931.0
42	46325.0
43	45758.0
44	45191.0
45	48221.0
46	51251.0
47	53681.0
48	56111.0
49	52388.5
50	48666.0
51	43615.5
52	38565.0
53	36001.0
54	33437.0
55	32368.0
56	31299.0
57	30188.0
58	29077.0
59	26502.0
60	23927.0
61	20944.5
62	17962.0
63	15507.0
64	13052.0
65	10907.0
66	8762.0
67	7470.5
68	6179.0
69	5352.0
70	4525.0
71	3638.5
72	2752.0
73	2279.0
74	1806.0
75	1430.0
76	1054.0
77	892.0
78	730.0
79	620.5
80	511.0
81	397.0
82	283.0
83	225.0
84	167.0
85	135.5
86	104.0
87	70.5
88	37.0
89	26.5
90	16.0
91	9.5
92	3.0
93	3.0
94	3.0
95	3.5
96	4.0
97	6.0
98	8.0
99	5.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030965496503585984
2	0.003710741316545428
3	1.2795659712225613E-4
4	0.0
5	0.0
6	2.5591319424451227E-4
7	0.0
8	0.0
9	0.0
10	2.5591319424451227E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0012795659712225614
17	0.0
18	2.5591319424451227E-4
19	0.0
20	2.5591319424451227E-4
21	0.0
22	0.0
23	0.0
24	0.001023652776978049
25	0.0
26	6.397829856112807E-4
27	0.0011516093741003053
28	0.0024311753453228665
29	6.397829856112807E-4
30	0.002559131942445123
31	7.677395827335367E-4
32	0.0014075225683448174
33	3.8386979136676835E-4
34	0.0019193489568338417
35	3.8386979136676835E-4
36	7.677395827335367E-4
37	0.0029430017338118912
38	0.0
39	0.0015354791654670734
40	0.0
41	0.0024311753453228665
42	0.006781699647479575
43	0.005246220482012501
44	0.005118263884890246
45	0.0034548281223009156
46	0.0038386979136676835
47	0.0012795659712225614
48	0.00563009027337927
49	0.0034548281223009156
50	0.0030709583309341468
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	781515.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.22522979173828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.55854584576622	49.3221548109532
2	11.975165168661565	14.663644784906493
3	3.4493656991630512	6.335646227009933
4	1.3532028129466247	3.314006127099349
5	0.7201241924438972	2.204488458048379
6	0.4130650123033335	1.5174000178319274
7	0.26284404891919894	1.1264881106128195
8	0.2089783408364586	1.0235797551366705
9	0.14120742266301223	0.7780911210757832
>10	0.7997812832780613	9.11876371084645
>50	0.07422920138994736	3.141971880955751
>100	0.038681873809509444	4.454848744531307
>500	0.0033454593524448735	1.4478674463723509
>1k	0.0014636384666946321	1.5510488046195758
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	2103	0.26909272374810467	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	1962	0.25105084355386653	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	1942	0.2484917116114214	No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA	1684	0.21547890955387933	No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC	1652	0.21138429844596712	No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA	1532	0.19602950679129638	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1241	0.15879413702871986	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	959	0.12271037664024363	No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA	903	0.11554480720139729	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	872	0.11157815269060734	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	824	0.10543623602873906	No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA	782	0.1000620589496043	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.2795659712225613E-4	0.0
11	0.0	0.0	0.0	1.2795659712225613E-4	0.0
12	0.0	0.0	0.0	1.2795659712225613E-4	1.2795659712225613E-4
13	0.0	0.0	0.0	2.5591319424451227E-4	1.2795659712225613E-4
14	0.0	0.0	0.0	3.838697913667684E-4	1.2795659712225613E-4
15	0.0	0.0	0.0	5.118263884890245E-4	1.2795659712225613E-4
16	0.0	0.0	0.0	0.001023652776978049	1.2795659712225613E-4
17	0.0	0.0	0.0	0.001023652776978049	1.2795659712225613E-4
18	0.0	0.0	0.0	0.002047305553956098	1.2795659712225613E-4
19	0.0	0.0	0.0	0.002047305553956098	1.2795659712225613E-4
20	0.0	0.0	0.0	0.003070958330934147	1.2795659712225613E-4
21	0.0	0.0	0.0	0.004478480899278965	1.2795659712225613E-4
22	0.0	0.0	0.0	0.005758046870501526	1.2795659712225613E-4
23	0.0	0.0	0.0	0.008445135410068905	1.2795659712225613E-4
24	0.0	0.0	0.0	0.012283833323736589	1.2795659712225613E-4
25	0.0	0.0	0.0	0.01420318228057043	1.2795659712225613E-4
26	0.0	0.0	0.0	0.01816983679136037	1.2795659712225613E-4
27	0.0	0.0	0.0	0.02149670831653903	1.2795659712225613E-4
28	0.0	0.0	0.0	0.027254755187040555	2.5591319424451227E-4
29	0.0	0.0	0.0	0.03288484546041982	2.5591319424451227E-4
30	0.0	0.0	0.0	0.03800310934531007	2.5591319424451227E-4
31	0.0	0.0	0.0	0.047343940935234766	2.5591319424451227E-4
32	0.0	0.0	0.0	0.06090734023019392	2.5591319424451227E-4
33	0.0	0.0	0.0	0.07344708674817502	2.5591319424451227E-4
34	0.0	0.0	0.0	0.08931370479133478	2.5591319424451227E-4
35	0.0	0.0	0.0	0.1053082794316168	2.5591319424451227E-4
36	0.0	0.0	0.0	0.12718885753952258	2.5591319424451227E-4
37	0.0	0.0	0.0	0.16096939917979822	2.5591319424451227E-4
38	0.0	0.0	0.0	0.19270263526611772	2.5591319424451227E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAA	25	0.0023521013	35.20056	20
CGCGAAA	25	0.0023521013	35.20056	21
GCGATTA	30	0.005741543	29.335678	42
GGCGCGA	30	0.005743339	29.333801	19
TCGCGAG	30	0.005743339	29.333801	18
TATCGCG	30	0.0057451352	29.331924	16
ATCGCGA	30	0.0057451352	29.331924	17
TAGGACC	1045	0.0	25.472462	4
AATAGCG	45	0.0013981629	24.443272	5
ACGCTAT	85	2.351535E-7	23.293001	10
CTATACT	95	2.683555E-8	23.156784	4
TGTCGAG	125	4.0017767E-11	22.880367	18
TATGTCG	125	4.0017767E-11	22.878902	16
CGAATGC	70	3.2099077E-5	22.003168	43
TCGAGGA	110	5.2459654E-9	22.000353	20
TACGCTA	90	4.079866E-7	21.998943	9
CGATGGA	50	0.0025803484	21.998943	31
ACCGTCG	50	0.0025803484	21.998943	8
ATTACCG	95	6.8630834E-7	20.841105	27
AGGACCT	1865	0.0	20.524485	5
>>END_MODULE