FastQCFastQC Report
Thu 2 Feb 2017
SRR4061949_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061949_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901741
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTAGAGTGATCGAAAGATGAAA43210.4791841559827046No Hit
GTCCTAAGGGGTAGGGGAATTCCGA39430.4372652457856524No Hit
CTCTACTTGTGCGCTATCGGTCTCT32370.35897225478269257No Hit
CTGTAGGACGTGGAATATGGCAAGA27760.30784892779634065No Hit
GTCCTACAGTGGACATTTCTAAATT27190.30152782229043595No Hit
GTTCTAAGTTGGTCGTTAAGCGTGC23330.2587217393908007No Hit
CCCTTGGGGGTTCCCACCTCCGTTC22320.24752118402068887No Hit
ATCTAGTAGCTGGTTCCTGCCGAAG22040.24441607956164796No Hit
GGTTAAGGTTGTTTCAACCCCAAGG21890.24275263074430464No Hit
GTCCTAAAGTGTGTATTTCTCATTT21610.2396475262852637No Hit
CTACTAGATAGTTCGATTAGTCTTT21450.23787318088009748No Hit
ATCCTGATGTCTTCGGATGGATTTG19850.22012972682843523No Hit
CATCAGGATCGGTCGATGGTGCACC18480.20493689429669937No Hit
ATGTAGGTAAGGGAAGTCGGCAAAA17430.19329275257529602No Hit
ATTCAGGCATAGTTCACCATCTTTC16500.1829793699077673No Hit
CTTTAGGACGTGAAATATGGCGAGG15600.17299867700370727No Hit
GTGCTGGAGGGCGCCGGCCGGTTGC15480.1716679179498326No Hit
GGTAGGGATACCCGCTGAACTTAAG15440.17122433159854103No Hit
ACCTTAACCTATTCTCAAACTTTAA15280.1694499861933748No Hit
CTCCTTAGCGGATTCCGACTTCCAT15180.16834102031514592No Hit
CTGCTATCCTGAGGGAAACTTCGGC15040.16678846808562547No Hit
GGCTATAACGCACCCCGAGGGGTGC14870.16490322609263636No Hit
CTCCACGGCAACGTAACCGAAGGCG14680.16279619092400147No Hit
GCTAAATACCGGCCAGAGACCGATA14500.16080005234318945No Hit
GTCTAACATCTACGCGAGTGTTTGG14000.155255222952045No Hit
GTATTTAGCTTTAGAAGGAATTTAC13990.1551443263642221No Hit
CCCCCAGGGGGTCCGAGTTGTAATT13990.1551443263642221No Hit
ATGTTAGACTCCTTGGTCCGTGTTT13920.15436805024946187No Hit
GTTATAGCCCGGGGCGCAATGCGGC12890.1429457017037043No Hit
CCCCCAAGGGTGCACCATCGACCGA12870.1427239085280585No Hit
GGGTAGGGGAATTCCGATTCAACGT12630.14006239042030916No Hit
GCCTCTAGTTGATAGAACAATGTAG12350.13695728596126827No Hit
CCGCAAAGGTGCGGCGCTCTGCCGG12350.13695728596126827No Hit
GTTCTGACGTGCAAATCGATCGTCA12330.1367354927856225No Hit
GTATCAACGCAGAGTACATGGGCAG12270.13607011325868515No Hit
CCCCGAGGGGTGCTACGTTCCCGGG12190.13518294055610203No Hit
CGTCAGAACCGCTGCGAGCCTCCAC12100.13418487126569603No Hit
GTCTAGATGAACTAACGCCTTTTGT11970.13274321562399846No Hit
CCTTAACCCCGCGTTCGGTTCATCC11700.12974900775278045No Hit
TCCCACCTCCGTTCACTTTCATTGC11640.1290836282258431No Hit
TTATAGCCCGGGGCGCAATGCGGCC11030.12231893636864687No Hit
GGGTTAAGGTGCCGGAATGCACGCT11030.12231893636864687No Hit
GCTCTAAGCGGGAGGTAAATTCCTT10950.12143176366606376No Hit
GGATAGCAGTAACGACCTTCAGTTT10880.12065548755130354No Hit
GTACATGGGGTTGACCTCGGATCAG10850.12032279778783486No Hit
GTGCTACGTTCCCGGGGCCTTTGTC10550.11699590015314819No Hit
GGTTTGACACCCAAACACTCGCGTA10480.11621962403838795No Hit
CCTTAGCGGATTCCGACTTCCATGG10460.1159978308627422No Hit
CCCCTTGTCCGTACCAGTTCTAAGT10370.11499976157233618No Hit
TGCCTGAATAGGGTGAAGCCAGAGG10330.11455617522104462No Hit
GTTTGGGAATGCAGCTCTAAGCGGG10310.11433438204539884No Hit
CCTTTGCGGCGTCCGGTGCGCTCCC10280.11400169228193019No Hit
CTCTTACTCAAATCCATCCGAAGAC9890.10967672535683748No Hit
GATCTGCACCAGGGGCCGTTCGACC9840.10912224241772304No Hit
TTCCCAAACAACTCGACTCGTCGAA9760.10823506971513992No Hit
GAGTAAGAGCGTAGCTGTTGGGACC9720.10779148336384836No Hit
ACTAGAGGCTGTTCACCTTGGAGAC9540.10579534478303637No Hit
GTTACACACTCCTTAGCGGATTCCG9400.10424279255351593No Hit
ACGTAGCACCCCTCGGGGTGCGTTA9350.10368830961440148No Hit
GAATAGGGTGAAGCCAGAGGAAACT9350.10368830961440148No Hit
GCTTAGAGCTGCATTCCCAAACAAC9110.10102679150665213No Hit
GTTTTAATTAGACAGTCAGATTCCC9100.10091589491882924No Hit
GTCCGTACCAGTTCTAAGTTGGTCG9070.10058320515536057No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCG451.6663944E-619.00055
CGTTAGA250.006038912618.9952242
AGTATGG402.758945E-416.62543714
AGGATCG2650.015.0569995
CGGTCGA2600.014.9836610
GACCTAG456.7542266E-414.7789877
CCGGTTG2850.014.66705218
GGATCGG2600.014.6157686
TAGGACC3200.014.546454
CAGGATC2900.014.4133724
GGTATCA3100.014.3964081
TCGGTCG2650.014.342399
GGCGCCG3200.014.252748510
GGACCTA602.5652576E-514.2503756
AAGGGAT400.005275900514.2503745
CCCCTAG400.00529342314.2432561
TAAGGTG1750.014.1146575
GATCGGT2700.014.0752267
TCGGATG2850.014.00036813
TCTTCGG2650.013.9838310
Produced by FastQC (version 0.11.5)