##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4061949_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	901741
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.332266138503183	32.0	32.0	32.0	32.0	32.0
2	31.025537266243855	32.0	32.0	32.0	32.0	32.0
3	31.06008488024832	32.0	32.0	32.0	32.0	32.0
4	31.023069817164796	32.0	32.0	32.0	32.0	32.0
5	31.042071947488246	32.0	32.0	32.0	32.0	32.0
6	34.55833881347305	36.0	36.0	36.0	32.0	36.0
7	34.510484717895714	36.0	36.0	36.0	32.0	36.0
8	34.498681994053726	36.0	36.0	36.0	32.0	36.0
9	34.56415533950436	36.0	36.0	36.0	32.0	36.0
10	34.36081313814055	36.0	36.0	36.0	32.0	36.0
11	34.518767584040205	36.0	36.0	36.0	32.0	36.0
12	34.43354466526419	36.0	36.0	36.0	32.0	36.0
13	34.4675400142613	36.0	36.0	36.0	32.0	36.0
14	34.42743759017279	36.0	36.0	36.0	32.0	36.0
15	34.32696084574174	36.0	36.0	36.0	32.0	36.0
16	34.391503768820535	36.0	36.0	36.0	32.0	36.0
17	34.29515126849062	36.0	36.0	36.0	32.0	36.0
18	34.315579528933476	36.0	36.0	36.0	32.0	36.0
19	34.27038695146389	36.0	36.0	36.0	32.0	36.0
20	34.24425971537282	36.0	36.0	36.0	32.0	36.0
21	34.244870755571725	36.0	36.0	36.0	32.0	36.0
22	34.182891761603386	36.0	36.0	36.0	32.0	36.0
23	34.18190034610825	36.0	36.0	36.0	32.0	36.0
24	34.10360846407117	36.0	36.0	36.0	32.0	36.0
25	33.71636423318891	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	13.0
5	53.0
6	194.0
7	38.0
8	161.0
9	127.0
10	69.0
11	20.0
12	37.0
13	44.0
14	114.0
15	244.0
16	386.0
17	700.0
18	959.0
19	1291.0
20	1659.0
21	2064.0
22	2468.0
23	3109.0
24	4035.0
25	5545.0
26	7670.0
27	10132.0
28	13575.0
29	18467.0
30	25450.0
31	36927.0
32	55302.0
33	88004.0
34	218932.0
35	403952.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.906239014160576	18.38035596060457	11.532289634690688	27.181115390544164
2	17.383208869982187	18.65841297583112	38.33951768695563	25.61886046723106
3	19.10113480981705	21.7083251546737	29.35401342076459	29.836526614744653
4	13.697735288246124	15.22392032473438	35.343033959585654	35.735310427433845
5	14.869792388886733	35.49822784529283	34.142071895678605	15.489907870141828
6	33.40250136764068	36.60882813444935	15.960283492771202	14.028387005138779
7	31.020640164837115	31.134209918647198	18.966481935415114	18.878667981100573
8	28.029401446759	34.03136420759082	18.373572890809218	19.565661454840956
9	28.744879039868547	13.583950439098045	18.942167845366434	38.729002675666976
10	18.98531348481046	24.654490458095793	29.811094059878695	26.549101997215047
11	37.249678231098365	20.908148998712925	21.9116015595936	19.930571210595108
12	24.95988486818362	22.52188426187602	28.393073879580204	24.125156990360153
13	29.337446256617987	20.232828767366662	25.494905482713285	24.934819493302065
14	23.627497893326257	19.976152120163114	23.79070265757309	32.605647328937536
15	27.458463902849616	27.517761525836665	19.79274703456363	25.23102753675009
16	24.787214907946716	27.13223652509121	23.63863062550934	24.441917941452733
17	22.83849864094228	27.792286237097834	24.905843059922898	24.463372062036985
18	24.159737102128272	25.172138154607932	27.233244146414577	23.434880596849222
19	27.38542691143855	24.10491682447778	24.162750064937516	24.346906199146154
20	27.89223701108776	23.73352070089677	24.26443246387236	24.10980982414311
21	24.485376306608604	23.470392418256832	26.104200128537986	25.940031146596574
22	26.140488766626923	25.538355991021294	23.29490074356293	25.026254498788852
23	24.097237328692625	25.740056736203293	27.091648057961493	23.07105787714259
24	27.013649850314525	24.4953595837001	24.760653088227297	23.73033747775808
25	24.910316290468664	25.70670319131933	24.22869789909251	25.1542826191195
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	32.0
1	32.0
2	96.0
3	160.0
4	160.0
5	160.0
6	376.5
7	593.0
8	593.0
9	593.0
10	728.0
11	863.0
12	863.0
13	863.0
14	1224.0
15	1585.0
16	1585.0
17	1585.0
18	3623.0
19	5661.0
20	5661.0
21	5661.0
22	8617.5
23	11574.0
24	11574.0
25	11574.0
26	19317.5
27	27061.0
28	27061.0
29	27061.0
30	37323.5
31	47586.0
32	47586.0
33	47586.0
34	54602.5
35	61619.0
36	61619.0
37	61619.0
38	69591.5
39	77564.0
40	77564.0
41	77564.0
42	84766.0
43	91968.0
44	91968.0
45	91968.0
46	104262.5
47	116557.0
48	116557.0
49	116557.0
50	117185.0
51	117813.0
52	117813.0
53	117813.0
54	108005.0
55	98197.0
56	98197.0
57	98197.0
58	86887.5
59	75578.0
60	75578.0
61	75578.0
62	61521.0
63	47464.0
64	47464.0
65	47464.0
66	46593.5
67	45723.0
68	45723.0
69	45723.0
70	36234.0
71	26745.0
72	26745.0
73	26745.0
74	25802.0
75	24859.0
76	24859.0
77	24859.0
78	19715.5
79	14572.0
80	14572.0
81	14572.0
82	10139.0
83	5706.0
84	5706.0
85	5706.0
86	3598.0
87	1490.0
88	1490.0
89	1490.0
90	934.0
91	378.0
92	378.0
93	378.0
94	238.0
95	98.0
96	98.0
97	98.0
98	196.5
99	295.0
100	295.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013307590538746712
2	3.326897634686678E-4
3	4.4358635129155714E-4
4	0.00898262361365403
5	0.03282538999557522
6	0.06143670965388066
7	0.10790237995167128
8	0.10745879360037972
9	0.11433438204539884
10	0.13163424974576957
11	0.13862073477861162
12	0.13396307809005026
13	0.12919452481366603
14	0.11444527863322175
15	0.13296500879964426
16	0.11832665920702287
17	0.12387148859816731
18	0.11211645028894107
19	0.09703451434502812
20	0.09293134059558121
21	0.093153133771227
22	0.1045754823169846
23	0.08084361252288629
24	0.09448389282510167
25	0.08838458049484275
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	901741.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.62226284479351
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.40070278664442	11.655762032449953
2	14.32734156197005	5.622697239922215
3	7.405068398700937	4.359125955089518
4	4.3728615786695695	3.4322175712221195
5	2.992360323170289	2.9358440393789333
6	2.099356718332308	2.471647759925981
7	1.4560361667038304	1.9999507062211697
8	1.1520345196886803	1.8084419321285385
9	0.9085132298009316	1.6044376853813553
>10	4.886110727971558	17.135641287503287
>50	0.39531476706324625	5.517672975560461
>100	0.4904621608547951	20.959155847824228
>500	0.08438667663667954	11.144143571320829
>1k	0.02945038379266668	9.353261396071423
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CAAGTAGAGTGATCGAAAGATGAAA	4321	0.4791841559827046	No Hit
GTCCTAAGGGGTAGGGGAATTCCGA	3943	0.4372652457856524	No Hit
CTCTACTTGTGCGCTATCGGTCTCT	3237	0.35897225478269257	No Hit
CTGTAGGACGTGGAATATGGCAAGA	2776	0.30784892779634065	No Hit
GTCCTACAGTGGACATTTCTAAATT	2719	0.30152782229043595	No Hit
GTTCTAAGTTGGTCGTTAAGCGTGC	2333	0.2587217393908007	No Hit
CCCTTGGGGGTTCCCACCTCCGTTC	2232	0.24752118402068887	No Hit
ATCTAGTAGCTGGTTCCTGCCGAAG	2204	0.24441607956164796	No Hit
GGTTAAGGTTGTTTCAACCCCAAGG	2189	0.24275263074430464	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	2161	0.2396475262852637	No Hit
CTACTAGATAGTTCGATTAGTCTTT	2145	0.23787318088009748	No Hit
ATCCTGATGTCTTCGGATGGATTTG	1985	0.22012972682843523	No Hit
CATCAGGATCGGTCGATGGTGCACC	1848	0.20493689429669937	No Hit
ATGTAGGTAAGGGAAGTCGGCAAAA	1743	0.19329275257529602	No Hit
ATTCAGGCATAGTTCACCATCTTTC	1650	0.1829793699077673	No Hit
CTTTAGGACGTGAAATATGGCGAGG	1560	0.17299867700370727	No Hit
GTGCTGGAGGGCGCCGGCCGGTTGC	1548	0.1716679179498326	No Hit
GGTAGGGATACCCGCTGAACTTAAG	1544	0.17122433159854103	No Hit
ACCTTAACCTATTCTCAAACTTTAA	1528	0.1694499861933748	No Hit
CTCCTTAGCGGATTCCGACTTCCAT	1518	0.16834102031514592	No Hit
CTGCTATCCTGAGGGAAACTTCGGC	1504	0.16678846808562547	No Hit
GGCTATAACGCACCCCGAGGGGTGC	1487	0.16490322609263636	No Hit
CTCCACGGCAACGTAACCGAAGGCG	1468	0.16279619092400147	No Hit
GCTAAATACCGGCCAGAGACCGATA	1450	0.16080005234318945	No Hit
GTCTAACATCTACGCGAGTGTTTGG	1400	0.155255222952045	No Hit
GTATTTAGCTTTAGAAGGAATTTAC	1399	0.1551443263642221	No Hit
CCCCCAGGGGGTCCGAGTTGTAATT	1399	0.1551443263642221	No Hit
ATGTTAGACTCCTTGGTCCGTGTTT	1392	0.15436805024946187	No Hit
GTTATAGCCCGGGGCGCAATGCGGC	1289	0.1429457017037043	No Hit
CCCCCAAGGGTGCACCATCGACCGA	1287	0.1427239085280585	No Hit
GGGTAGGGGAATTCCGATTCAACGT	1263	0.14006239042030916	No Hit
GCCTCTAGTTGATAGAACAATGTAG	1235	0.13695728596126827	No Hit
CCGCAAAGGTGCGGCGCTCTGCCGG	1235	0.13695728596126827	No Hit
GTTCTGACGTGCAAATCGATCGTCA	1233	0.1367354927856225	No Hit
GTATCAACGCAGAGTACATGGGCAG	1227	0.13607011325868515	No Hit
CCCCGAGGGGTGCTACGTTCCCGGG	1219	0.13518294055610203	No Hit
CGTCAGAACCGCTGCGAGCCTCCAC	1210	0.13418487126569603	No Hit
GTCTAGATGAACTAACGCCTTTTGT	1197	0.13274321562399846	No Hit
CCTTAACCCCGCGTTCGGTTCATCC	1170	0.12974900775278045	No Hit
TCCCACCTCCGTTCACTTTCATTGC	1164	0.1290836282258431	No Hit
TTATAGCCCGGGGCGCAATGCGGCC	1103	0.12231893636864687	No Hit
GGGTTAAGGTGCCGGAATGCACGCT	1103	0.12231893636864687	No Hit
GCTCTAAGCGGGAGGTAAATTCCTT	1095	0.12143176366606376	No Hit
GGATAGCAGTAACGACCTTCAGTTT	1088	0.12065548755130354	No Hit
GTACATGGGGTTGACCTCGGATCAG	1085	0.12032279778783486	No Hit
GTGCTACGTTCCCGGGGCCTTTGTC	1055	0.11699590015314819	No Hit
GGTTTGACACCCAAACACTCGCGTA	1048	0.11621962403838795	No Hit
CCTTAGCGGATTCCGACTTCCATGG	1046	0.1159978308627422	No Hit
CCCCTTGTCCGTACCAGTTCTAAGT	1037	0.11499976157233618	No Hit
TGCCTGAATAGGGTGAAGCCAGAGG	1033	0.11455617522104462	No Hit
GTTTGGGAATGCAGCTCTAAGCGGG	1031	0.11433438204539884	No Hit
CCTTTGCGGCGTCCGGTGCGCTCCC	1028	0.11400169228193019	No Hit
CTCTTACTCAAATCCATCCGAAGAC	989	0.10967672535683748	No Hit
GATCTGCACCAGGGGCCGTTCGACC	984	0.10912224241772304	No Hit
TTCCCAAACAACTCGACTCGTCGAA	976	0.10823506971513992	No Hit
GAGTAAGAGCGTAGCTGTTGGGACC	972	0.10779148336384836	No Hit
ACTAGAGGCTGTTCACCTTGGAGAC	954	0.10579534478303637	No Hit
GTTACACACTCCTTAGCGGATTCCG	940	0.10424279255351593	No Hit
ACGTAGCACCCCTCGGGGTGCGTTA	935	0.10368830961440148	No Hit
GAATAGGGTGAAGCCAGAGGAAACT	935	0.10368830961440148	No Hit
GCTTAGAGCTGCATTCCCAAACAAC	911	0.10102679150665213	No Hit
GTTTTAATTAGACAGTCAGATTCCC	910	0.10091589491882924	No Hit
GTCCGTACCAGTTCTAAGTTGGTCG	907	0.10058320515536057	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGTCCG	45	1.6663944E-6	19.0005	5
CGTTAGA	25	0.0060389126	18.995224	2
AGTATGG	40	2.758945E-4	16.625437	14
AGGATCG	265	0.0	15.056999	5
CGGTCGA	260	0.0	14.98366	10
GACCTAG	45	6.7542266E-4	14.778987	7
CCGGTTG	285	0.0	14.667052	18
GGATCGG	260	0.0	14.615768	6
TAGGACC	320	0.0	14.54645	4
CAGGATC	290	0.0	14.413372	4
GGTATCA	310	0.0	14.396408	1
TCGGTCG	265	0.0	14.34239	9
GGCGCCG	320	0.0	14.2527485	10
GGACCTA	60	2.5652576E-5	14.250375	6
AAGGGAT	40	0.0052759005	14.250374	5
CCCCTAG	40	0.005293423	14.243256	1
TAAGGTG	175	0.0	14.114657	5
GATCGGT	270	0.0	14.075226	7
TCGGATG	285	0.0	14.000368	13
TCTTCGG	265	0.0	13.98383	10
>>END_MODULE