FastQCFastQC Report
Thu 2 Feb 2017
SRR4061949_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4061949_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901741
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGTAGAGTGATCGAAAGATGAAAAGCACTTTGGAAAGAGAGTCAAAAA44580.4943769885144404No Hit
GTCCTAAGGGGTAGGGGAATTCCGATTCAACGTGCGCGCTCGCCGCGCCG33050.36651322275464904No Hit
CTCTACTTGTGCGCTATCGGTCTCTGGCCGGTATTTAGCTTTAGAAGGAA30930.34300314613619654No Hit
GTTCTAAGTTGGTCGTTAAGCGTGCGCCGGACGGCCGAAGCCTGCCGAGG24010.26626270736275715No Hit
GGTTAAGGTTGTTTCAACCCCAAGGCCTCTAATCATTCGCTTTACCTCAT22640.25106987483102133No Hit
CCCTTGGGGGTTCCCACCTCCGTTCACTTTCATTGCGCGCACGGGTTTGA21250.23565524912363972No Hit
CTACTAGATAGTTCGATTAGTCTTTCGCCCCTATACCCAAATTTGACGAT20960.2324392480767759No Hit
ATCTAGTAGCTGGTTCCTGCCGAAGTTTCCCTCAGGATAGCAGTAACGAC20890.2316629719620157No Hit
CATCAGGATCGGTCGATGGTGCACCCTTGGGGGTTCCCACCTCCGTTCAC19520.21647013943027985No Hit
ACTAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGGCCTG19000.21070351686348962No Hit
ATCCTGATGTCTTCGGATGGATTTGAGTAAGAGCGTAGCTGTTGGGACCC18950.21014903392437517No Hit
ATGTAGGTAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA17440.1934036491631189No Hit
GTGCTGGAGGGCGCCGGCCGGTTGCCCGGCTAGTGCCTACCTGCTCCGGG16850.18686075048156842No Hit
TACCTACATTGTTCTATCAACTAGAGGCTGTTCACCTTGGAGACCTGCTG16660.18475371531293355No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA16370.18153771426606974No Hit
GCTAAATACCGGCCAGAGACCGATAGCGCACAAGTAGAGTGATCGAAAGA15910.17643647122621683No Hit
ATTCAGGCATAGTTCACCATCTTTCGGGTCCCAACAGCTACGCTCTTACT15670.1737749531184675No Hit
CCCCCAGGGGGTCCGAGTTGTAATTTGCAGAGGATGCTTCGGGCGAGGCC15030.1666775714978026No Hit
CTGCTATCCTGAGGGAAACTTCGGCAGGAACCAGCTACTAGATAGTTCGA14470.1604673625797208No Hit
GGCTATAACGCACCCCGAGGGGTGCTACGTTCCCGGGGCCTTTGTCCGGC14220.15769494788414856No Hit
ACCTTAACCTATTCTCAAACTTTAAATATGTAAGAAGTCCTTGTTTCTTA13710.1520392219051812No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGA13630.1511520492025981No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA13590.15070846285130654No Hit
GTCTAACATCTACGCGAGTGTTTGGGTGTCAAACCCGTGCGCGCAATGAA13500.14971039356090052No Hit
CCCCCAAGGGTGCACCATCGACCGATCCTGATGTCTTCGGATGGATTTGA13260.14704887545315118No Hit
GTCTAGATGAACTAACGCCTTTTGTGGTGTCTGATGAGCGTGCATTCCGG13210.14649439251403673No Hit
CGTCAGAACCGCTGCGAGCCTCCACCAGAGTTTCCTCTGGCTTCACCCTA13130.1456072198114536No Hit
GTTATAGCCCGGGGCGCAATGCGGCCAGCCCGGACCGAGGACCGCGCTTC12860.14261301194023562No Hit
GTATTTAGCTTTAGAAGGAATTTACCTCCCGCTTAGAGCTGCATTCCCAA12660.14039508018377783No Hit
ATGTTAGACTCCTTGGTCCGTGTTTCAAGACGGGTCGCTTACGACCATTA12530.13895342454208026No Hit
CCTTAACCCCGCGTTCGGTTCATCCCGCATCGCCAGTTCTGCTTACCAAA12310.1365136996099767No Hit
CCCCGAGGGGTGCTACGTTCCCGGGGCCTTTGTCCGGCCGCCCGAACCGA12120.1344066644413418No Hit
GTTCTGACGTGCAAATCGATCGTCAAATTTGGGTATAGGGGCGAAAGACT11990.13296500879964426No Hit
TCCCACCTCCGTTCACTTTCATTGCGCGCACGGGTTTGACACCCAAACAC11530.12786376575979133No Hit
TTATAGCCCGGGGCGCAATGCGGCCAGCCCGGACCGAGGACCGCGCTTCG11320.12553493741551067No Hit
GCTCTAAGCGGGAGGTAAATTCCTTCTAAAGCTAAATACCGGCCAGAGAC11280.1250913510642191No Hit
GGATAGCAGTAACGACCTTCAGTTTTATGAGGTAAAGCGAATGATTAGAG11060.12265162613211554No Hit
GTTTGGGAATGCAGCTCTAAGCGGGAGGTAAATTCCTTCTAAAGCTAAAT11050.12254072954429265No Hit
GCCTCTAGTTGATAGAACAATGTAGGTAAGGGAAGTCGGCAAAATAGATC10820.11999010802436619No Hit
GTACATGGGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGC10730.11899203873396019No Hit
CCGCAAAGGTGCGGCGCTCTGCCGGCCGTTAAACCCTATCTCCGGACAAA10690.11854845238266863No Hit
CCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGAAC10430.11566514109927352No Hit
GGGTAGGGGAATTCCGATTCAACGTGCGCGCTCGCCGCGCCGCCCCCCGA10170.11278182981587839No Hit
CCCCTTGTCCGTACCAGTTCTAAGTTGGTCGTTAAGCGTGCGCCGGACGG10170.11278182981587839No Hit
GTATCAACGCAGAGTACATGGGCAGAAATCACATTGCGTCAACACCACTT10160.1126709332280555No Hit
GTGCTACGTTCCCGGGGCCTTTGTCCGGCCGCCCGAACCGATGCTGGCCC10030.11122927758635795No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC9930.11012031170812904No Hit
GTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGT9930.11012031170812904No Hit
GGTTTGACACCCAAACACTCGCGTAGATGTTAGACTCCTTGGTCCGTGTT9920.11000941512030615No Hit
GAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGCAGCGGTTCTG9830.10901134582990016No Hit
CTCCACGGCAACGTAACCGAAGGCGGAGACGTCGGCGGGGGCCCTGGGAA9830.10901134582990016No Hit
GATCTGCACCAGGGGCCGTTCGACCCGGGGTCACCCCCGAGGCTTCGTCA9820.10890044924207727No Hit
GTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCAGTTCTAAGTTGG9720.10779148336384836No Hit
GTCCGTACCAGTTCTAAGTTGGTCGTTAAGCGTGCGCCGGACGGCCGAAG9700.1075696901882026No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCTTGTCCGTACCA9670.10723700042473393No Hit
CCTTGGAGACCTGCTGCGGTTATGAGTACGGCCTGGCGTGAAAACTGTTC9610.10657162089779659No Hit
GAGTAAGAGCGTAGCTGTTGGGACCCGAAAGATGGTGAACTATGCCTGAA9550.10590624137085924No Hit
CTCTTACTCAAATCCATCCGAAGACATCAGGATCGGTCGATGGTGCACCC9510.10546265501956771No Hit
TTGTTACACACTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCT9480.10512996525609904No Hit
TTCCCAAACAACTCGACTCGTCGAAGGAGCTTCACACGGGCTCGGCCGCC9460.10490817208045325No Hit
GTGCCTACCTGCTCCGGGAGCTTCTGCCCAAGGCCCAACGCACCCGACCC9400.10424279255351593No Hit
TGCCTGAATAGGGTGAAGCCAGAGGAAACTCTGGTGGAGGCTCGCAGCGG9390.10413189596569304No Hit
GGGTTAAGGTGCCGGAATGCACGCTCATCAGACACCACAAAAGGCGTTAG9280.10291203349964125No Hit
TTCCTGGACGGGCGGCGGGGTATTGGTAGCACGCTTGAGCGCCATCCATT9150.10147037785794369No Hit
ACCCGGGGTCACCCCCGAGGCTTCGTCACCGACCCCCGCGCCTGCCTACT9020.10002872221624613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4800.029.3398061
CTATAAG300.005745841729.3316692
GTATCAA11800.026.6668991
TATTGCG609.753683E-625.6652116
TGTAGGA11200.024.1593462
GCGCTAT6200.024.12766311
TGCGCTA6250.023.93464310
AGAGTCA10250.023.82832540
GCTATCG6300.023.74468613
GAGTCAA10300.023.71265441
TGTGCGC6150.023.6084188
TACTTGT6350.023.5577184
CGCTATC6400.023.37367412
CCGAGTT4250.023.29279713
CACTTTG12650.023.12912628
AAGCACT12500.023.05469325
TTGTGCG6400.023.0299437
AGTCAAA10750.022.92471742
TAGAACC1254.0017767E-1122.8787044
CTATCGG6550.022.838414
Produced by FastQC (version 0.11.5)