FastQCFastQC Report
Thu 2 Feb 2017
SRR4050287_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050287_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9181853
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA143040.15578554786272444No Hit
GTCCTAAAGTGTGTATTTCTCATTT114070.12423418235948669No Hit
GTATCTGATCGTCTTCGAACCTCCG103910.11316887778534464No Hit
CTTTAGGACGTGAAATATGGCGAGG103800.11304907625944349No Hit
CTGTAGGACGTGGAATATGGCAAGA100570.10953126781707352No Hit
GATTAAGAGGGACGGCCGGGGGCAT98070.1068085058647748No Hit
GTCCTACAGTGGACATTTCTAAATT97610.10630751766555183No Hit
GAATAGGACCGCGGTTCTATTTTGT97130.10578474737071046No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG92100.10030655032268541No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52800.015.90389251
GTATCAA165050.013.0605431
ACGCTTA3100.012.26834817
AACCGCG8450.011.9291187
GCGTTAT6850.011.7474091
CTCGACG3350.011.63712913
GGTAGCG5050.011.29926111
CGTCGTA15500.011.22818410
AGAACCG9800.011.062115
CGGTCCA15000.011.03184410
CGGTTCT18900.011.01723112
AGGCCCG17900.010.78532310
ATCGCCA14350.010.73408916
CCGTCGT16350.010.7025639
CGCCAGT14750.010.57157218
CGGTAGC5500.010.547688510
ATACCGT16850.010.4972276
GCATCGC14600.010.48510314
CATCGCC14850.010.43664315
TCGACGC3650.010.41997714
Produced by FastQC (version 0.11.5)