##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050287_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9181853
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.688848863078075	31.0	31.0	34.0	27.0	34.0
2	30.799222008890798	31.0	31.0	34.0	27.0	34.0
3	30.913379902727694	31.0	31.0	34.0	27.0	34.0
4	34.464776554362174	37.0	35.0	37.0	32.0	37.0
5	34.4878057838652	37.0	35.0	37.0	32.0	37.0
6	34.533080523070886	37.0	35.0	37.0	32.0	37.0
7	34.47926284596366	37.0	35.0	37.0	31.0	37.0
8	34.44953725571516	37.0	35.0	37.0	31.0	37.0
9	36.03860081401869	39.0	37.0	39.0	32.0	39.0
10	35.91410905837852	39.0	37.0	39.0	31.0	39.0
11	35.81493245426604	39.0	37.0	39.0	31.0	39.0
12	35.73868052559761	39.0	37.0	39.0	31.0	39.0
13	35.74896472422288	39.0	37.0	39.0	31.0	39.0
14	36.923126410322624	40.0	38.0	41.0	31.0	41.0
15	36.8864779255342	40.0	38.0	41.0	31.0	41.0
16	36.89908398664191	40.0	38.0	41.0	31.0	41.0
17	36.87160238788402	40.0	38.0	41.0	31.0	41.0
18	36.838119712872775	40.0	38.0	41.0	31.0	41.0
19	36.86269928303143	40.0	38.0	41.0	31.0	41.0
20	36.82286200835496	40.0	38.0	41.0	31.0	41.0
21	36.77213183439116	40.0	38.0	41.0	31.0	41.0
22	36.69491103810963	40.0	37.0	41.0	30.0	41.0
23	36.62245747127513	40.0	37.0	41.0	30.0	41.0
24	36.56716416610024	40.0	37.0	41.0	30.0	41.0
25	36.334496642453324	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4908864868961125
1101	2	0.4851927631414412
1101	3	0.4859903542555202
1101	4	0.491475556913862
1101	5	0.4859444065762375
1101	6	0.48551166664024237
1101	7	0.481423936495446
1101	8	0.4598149070337101
1101	9	0.5627606748737435
1101	10	0.5817177187807303
1101	11	0.46407051583547343
1101	12	0.5199400182096028
1101	13	0.4398282997623255
1101	14	0.5633500084003131
1101	15	0.6043098177232764
1101	16	0.6665313560007036
1101	17	0.6294023823207908
1101	18	0.6319426562413497
1101	19	0.7020189155399521
1101	20	0.6587905303560007
1101	21	0.6348840615843088
1101	22	0.6513841805459322
1101	23	0.6079423406732261
1101	24	0.626987006898851
1101	25	0.6632980200164269
1102	1	0.5201774803802728
1102	2	0.5149370562474047
1102	3	0.5308304388238341
1102	4	0.48919946199893616
1102	5	0.4699921237832214
1102	6	0.4803905750082862
1102	7	0.5094388537076497
1102	8	0.5385710791761795
1102	9	0.6495113639362131
1102	10	0.6616439618207792
1102	11	0.5656105436017071
1102	12	0.5222783822358039
1102	13	0.5542602518399704
1102	14	0.6821731599551981
1102	15	0.6016372016662288
1102	16	0.6576191327141743
1102	17	0.6355800665533096
1102	18	0.6945263224933882
1102	19	0.7509925332124894
1102	20	0.6763961225564117
1102	21	0.7521822964091811
1102	22	0.7487857194802956
1102	23	0.6983565396038429
1102	24	0.6574520201302505
1102	25	0.7444544634495855
1103	1	0.5548612959555861
1103	2	0.5132811473231698
1103	3	0.5021781443656224
1103	4	0.5195132999400798
1103	5	0.499940552838531
1103	6	0.47621975059299615
1103	7	0.5087638077284922
1103	8	0.5417611026782367
1103	9	0.5580755028803992
1103	10	0.624907238721427
1103	11	0.5598048442248995
1103	12	0.5458688579603077
1103	13	0.48822378730223903
1103	14	0.6374896672039156
1103	15	0.6675398325114728
1103	16	0.7024635929181571
1103	17	0.5556592791438888
1103	18	0.6403356336740131
1103	19	0.6907181131449178
1103	20	0.6754036958427037
1103	21	0.6009590582691331
1103	22	0.6688018973838865
1103	23	0.716912268323
1103	24	0.7159766874399338
1103	25	0.7501782807990054
1104	1	0.45866406062096843
1104	2	0.468831063537106
1104	3	0.47050305977171547
1104	4	0.4435582544996066
1104	5	0.4782680891269635
1104	6	0.43006031615510665
1104	7	0.4609180908395416
1104	8	0.4624453793149641
1104	9	0.5285930719417067
1104	10	0.47243627196267113
1104	11	0.4008032174025473
1104	12	0.39216275439971326
1104	13	0.3843849886554196
1104	14	0.4250303888040534
1104	15	0.38150325237425164
1104	16	0.43653881390481075
1104	17	0.4001990647578282
1104	18	0.5175547620720948
1104	19	0.5787134638135853
1104	20	0.5444212924537695
1104	21	0.4618476145108019
1104	22	0.4989718070836773
1104	23	0.5261712472395388
1104	24	0.5513287252729882
1104	25	0.5966937045579712
1105	1	0.3319993973401907
1105	2	0.37459485971638884
1105	3	0.4283239719388767
1105	4	0.45760603048970694
1105	5	0.45853312056711104
1105	6	0.3807936702070336
1105	7	0.41659893376365176
1105	8	0.4407692560575853
1105	9	0.4217981850056347
1105	10	0.4657796879199907
1105	11	0.3776402340910394
1105	12	0.3537227628479087
1105	13	0.37335713231295387
1105	14	0.479067594919087
1105	15	0.4918804022716259
1105	16	0.39047344721806354
1105	17	0.4349673564782819
1105	18	0.5466536940167472
1105	19	0.502234718573817
1105	20	0.4887397578514481
1105	21	0.5069219214410481
1105	22	0.5596889575068076
1105	23	0.47840042736081045
1105	24	0.464360670098543
1105	25	0.47816205065902295
1106	1	0.24081576298867446
1106	2	0.2570549230688641
1106	3	0.24903836229220389
1106	4	0.3097025423334401
1106	5	0.2958789317995283
1106	6	0.28569203355831263
1106	7	0.23898974350839808
1106	8	0.22267168735950804
1106	9	0.2427870911897969
1106	10	0.3294761188036688
1106	11	0.296071546474721
1106	12	0.30535770481125724
1106	13	0.24062869626457228
1106	14	0.24537186691978263
1106	15	0.3443160504348697
1106	16	0.22015529102700526
1106	17	0.3531574405923976
1106	18	0.31444438912098605
1106	19	0.26346061181503444
1106	20	0.3213928116577094
1106	21	0.3325671328448436
1106	22	0.32448091117969113
1106	23	0.17499774394541845
1106	24	0.35236967438631694
1106	25	0.3632989238201958
1107	1	0.2959571454355121
1107	2	0.28574967476355084
1107	3	0.21245597080201506
1107	4	0.2814420166266345
1107	5	0.3173306149540309
1107	6	0.21232750287300206
1107	7	0.3030191871913672
1107	8	0.2764239043849699
1107	9	0.39664273927132854
1107	10	0.3835514460856331
1107	11	0.31537536720566095
1107	12	0.30204925542659566
1107	13	0.3521181696345721
1107	14	0.35850496261127063
1107	15	0.4053481822443956
1107	16	0.3644856152808771
1107	17	0.4051931602249823
1107	18	0.37200548262633504
1107	19	0.3795072053545425
1107	20	0.3273246145542785
1107	21	0.33156649983762065
1107	22	0.34493475496754655
1107	23	0.3305410057728366
1107	24	0.33597843792042426
1107	25	0.4514919207431731
1108	1	0.19585601881952286
1108	2	0.15906846941165753
1108	3	0.1561973084507713
1108	4	0.2078359792609774
1108	5	0.1738768622496778
1108	6	0.1257730243125934
1108	7	0.13443403414830613
1108	8	0.08296704162321333
1108	9	0.06993583003597337
1108	10	0.014330782509759388
1108	11	0.01665584063358949
1108	12	0.01114950364930678
1108	13	0.04901221186737814
1108	14	-0.06988193964681955
1108	15	0.04900347772912994
1108	16	0.004046733737020247
1108	17	0.031355558653977766
1108	18	-0.017026012600439344
1108	19	0.036250413655906755
1108	20	0.03192093527247408
1108	21	0.03429966479251334
1108	22	0.10165228160182949
1108	23	0.046454305171920396
1108	24	0.04510749181194029
1108	25	0.035858338379775034
1109	1	-0.0941314140679097
1109	2	-0.05426562449477146
1109	3	0.013715631197459999
1109	4	0.035918311215162646
1109	5	-0.016150889075419173
1109	6	-0.02254136158875042
1109	7	-0.03324955609927116
1109	8	-0.011024666804644312
1109	9	-0.028182339638668452
1109	10	-0.152428492690035
1109	11	-0.07955869503327051
1109	12	-0.05491447232829216
1109	13	-0.06405288283205834
1109	14	-0.04167056793067303
1109	15	-0.04426704872736309
1109	16	-0.08115882613387271
1109	17	-0.06554538357291051
1109	18	-0.005693255665846664
1109	19	-0.08168247398029393
1109	20	-0.04884057357089944
1109	21	-0.01205067548261951
1109	22	0.007010095953511097
1109	23	0.03570124192462032
1109	24	-0.019109179465573334
1109	25	-0.004240139245496266
1110	1	-0.0390392594519291
1110	2	-0.058777463321856516
1110	3	-0.04297646840815972
1110	4	-0.06587783864436858
1110	5	-0.04739470442434879
1110	6	-0.022600129868386887
1110	7	-0.03331120190480874
1110	8	-0.0456271096310914
1110	9	0.012786809639479202
1110	10	-0.11983860410314406
1110	11	-0.0863506934979199
1110	12	-0.09733038569957841
1110	13	-0.06530745706087515
1110	14	-0.16234383339865843
1110	15	-0.12287946406630823
1110	16	-0.05820267324202888
1110	17	-0.07807616543259854
1110	18	-0.09611005347492352
1110	19	-0.15297610967598985
1110	20	-0.1176692339886003
1110	21	-0.16654829265664262
1110	22	-0.1471269735401961
1110	23	-0.08873423981344786
1110	24	-0.08179417777026998
1110	25	-0.06499322619934844
1111	1	-0.14869530416163457
1111	2	-0.19860410894171565
1111	3	-0.20789677583368515
1111	4	-0.15618218508228665
1111	5	-0.23830333579240914
1111	6	-0.19381378565739027
1111	7	-0.19676306973923374
1111	8	-0.21555811993034268
1111	9	-0.2224941384638086
1111	10	-0.2161991016511564
1111	11	-0.2798310929418548
1111	12	-0.3299410012169872
1111	13	-0.3091757060915725
1111	14	-0.3018220710764794
1111	15	-0.27248716763607916
1111	16	-0.32265105115356363
1111	17	-0.338124804026144
1111	18	-0.2737234324947906
1111	19	-0.21587742783902542
1111	20	-0.23546182300054141
1111	21	-0.2635759047296844
1111	22	-0.3082321655982483
1111	23	-0.32523867709968357
1111	24	-0.3826808957836505
1111	25	-0.3014809383202959
1112	1	-0.1787978261330565
1112	2	-0.15469894400540696
1112	3	-0.21159486133451821
1112	4	-0.2347243385676805
1112	5	-0.1849070788163658
1112	6	-0.225945898292224
1112	7	-0.27357884860904136
1112	8	-0.22870874106424566
1112	9	-0.20965988376656952
1112	10	-0.2740246651114475
1112	11	-0.2647206638098254
1112	12	-0.3147198154407036
1112	13	-0.3253268871945849
1112	14	-0.3155311213001184
1112	15	-0.2999666193576971
1112	16	-0.30130692474505594
1112	17	-0.2784134008163761
1112	18	-0.2883376011401353
1112	19	-0.43478792444712866
1112	20	-0.3859187168497016
1112	21	-0.3575808334541293
1112	22	-0.2342825305378895
1112	23	-0.2952602213253712
1112	24	-0.2907451508462344
1112	25	-0.23019065860292187
1113	1	-0.40532702797116826
1113	2	-0.37881577764915164
1113	3	-0.25702940933188856
1113	4	-0.14927241166156335
1113	5	-0.16934454006626254
1113	6	-0.2708255828860331
1113	7	-0.24239303457498806
1113	8	-0.27309754169224476
1113	9	-0.25892273499465546
1113	10	-0.37103599087265593
1113	11	-0.24838962050571212
1113	12	-0.4252063534483881
1113	13	-0.37630049756749884
1113	14	-0.3492149688249455
1113	15	-0.34522162763119724
1113	16	-0.34691392816445443
1113	17	-0.3541435560156003
1113	18	-0.2688808059646135
1113	19	-0.36144098950894943
1113	20	-0.26654258126826846
1113	21	-0.3256969291759404
1113	22	-0.36838484127797244
1113	23	-0.42577951801064984
1113	24	-0.43333951055262077
1113	25	-0.3596180602078789
1114	1	-0.47375242487993674
1114	2	-0.459089075743389
1114	3	-0.48755982127594777
1114	4	-0.4978294039313056
1114	5	-0.4906740713541353
1114	6	-0.47620257448559045
1114	7	-0.5872955304922627
1114	8	-0.5297779102404263
1114	9	-0.57776769358248
1114	10	-0.570262909907008
1114	11	-0.7216649019607289
1114	12	-0.7388763527739357
1114	13	-0.5717412660552128
1114	14	-0.8621718544104979
1114	15	-0.8332872629661736
1114	16	-0.8087674696627047
1114	17	-0.7896724404282409
1114	18	-0.8257582443980169
1114	19	-0.6594392028556797
1114	20	-0.8316880318465536
1114	21	-0.8228627082429725
1114	22	-0.913613217670239
1114	23	-0.8232311326109354
1114	24	-0.7802262667997937
1114	25	-0.8432138393269213
1115	1	-0.5102807615714475
1115	2	-0.5567522981624116
1115	3	-0.55964240341401
1115	4	-0.39582206075989745
1115	5	-0.40427605367759867
1115	6	-0.47665593989854926
1115	7	-0.5653675310770225
1115	8	-0.5729153382891852
1115	9	-0.49380792356163994
1115	10	-0.5468524444652161
1115	11	-0.6201135053526201
1115	12	-0.6544549907621899
1115	13	-0.5936923705683625
1115	14	-0.6970131074019719
1115	15	-0.6760258160565016
1115	16	-0.7090866689388733
1115	17	-0.695316369441862
1115	18	-0.6412529882901978
1115	19	-0.624645546813241
1115	20	-0.6813483354631984
1115	21	-0.7693888377601752
1115	22	-0.9933099427943759
1115	23	-0.83547424440642
1115	24	-0.6850715735840467
1115	25	-0.7125683200204946
1116	1	-0.17395047089921434
1116	2	-0.25066239637914833
1116	3	-0.18888886289193252
1116	4	-0.1548347563990049
1116	5	-0.2147025026065137
1116	6	-0.26094309137744887
1116	7	-0.14546240459455362
1116	8	-0.192871380418417
1116	9	-0.20258512666092088
1116	10	-0.16114717497202236
1116	11	-0.31945673402587715
1116	12	-0.37419965376616915
1116	13	-0.3641816518854739
1116	14	-0.2721527294745343
1116	15	-0.2934859847227145
1116	16	-0.25409627869935747
1116	17	-0.35702446968294765
1116	18	-0.23621441419350475
1116	19	-0.36279848135686166
1116	20	-0.274895615190097
1116	21	-0.3824345265419353
1116	22	-0.35580912018807
1116	23	-0.2914695955758191
1116	24	-0.3506462610795964
1116	25	-0.3706540365793387
1201	1	0.4045329679585876
1201	2	0.4705686091312131
1201	3	0.46696062786156745
1201	4	0.5095561066405168
1201	5	0.5257237861872639
1201	6	0.537147349530045
1201	7	0.5411637182359996
1201	8	0.5636153046104297
1201	9	0.6153630713965015
1201	10	0.6092993861667608
1201	11	0.5257934438406622
1201	12	0.5551216662750775
1201	13	0.6152754034644019
1201	14	0.7050183181909659
1201	15	0.7067468940646933
1201	16	0.5816342609253482
1201	17	0.6186761050232121
1201	18	0.6328315824517077
1201	19	0.5900624886621699
1201	20	0.5827551392953296
1201	21	0.6408760231382473
1201	22	0.6311686882502627
1201	23	0.664450841127092
1201	24	0.6572922392809133
1201	25	0.554066534492641
1202	1	0.37294021459743476
1202	2	0.33624092414335394
1202	3	0.38268789610036436
1202	4	0.4444807763002103
1202	5	0.47232925745080223
1202	6	0.4331021399258219
1202	7	0.41263444559210427
1202	8	0.37513195172250136
1202	9	0.42178114742563366
1202	10	0.48856686170343977
1202	11	0.3842564959850989
1202	12	0.37546274975767346
1202	13	0.2980070841635083
1202	14	0.4252689761698747
1202	15	0.43925283484636424
1202	16	0.4224202428899986
1202	17	0.43700034021838263
1202	18	0.5100755916258919
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2210	9	-0.3036448578233575
2210	10	-0.22526351348016505
2210	11	-0.1978727380647527
2210	12	-0.15164916783911764
2210	13	-0.09234475298354283
2210	14	-0.1477607693584133
2210	15	-0.15030742230948846
2210	16	-0.08064872095607711
2210	17	-0.16005346753729555
2210	18	-0.1037352396384108
2210	19	-0.042779549134216666
2210	20	-0.17125242447951194
2210	21	-0.15905528256869417
2210	22	-0.18629299624512186
2210	23	-0.16664787400571157
2210	24	-0.0656795502377463
2210	25	-0.13883086353882845
2211	1	-0.19043080046493444
2211	2	-0.20873485543566872
2211	3	-0.18916135623344843
2211	4	-0.2859468515300705
2211	5	-0.32219461565546226
2211	6	-0.247670382703717
2211	7	-0.19238398303106408
2211	8	-0.24815798479288276
2211	9	-0.2146230031770031
2211	10	-0.2816020187323858
2211	11	-0.10360989455161018
2211	12	-0.17921002801448793
2211	13	-0.10699581504657374
2211	14	-0.14258661220862479
2211	15	-0.12092273030093281
2211	16	-0.1534732516048649
2211	17	-0.2553063311435295
2211	18	-0.1676710408672335
2211	19	-0.2244504531179814
2211	20	-0.21972012177104716
2211	21	-0.23923498411121358
2211	22	-0.26702399324482684
2211	23	-0.1613769420009774
2211	24	-0.3531947481956337
2211	25	-0.3253895469634003
2212	1	-0.35140828390532164
2212	2	-0.30271596754742447
2212	3	-0.41655632582477864
2212	4	-0.3316079964543448
2212	5	-0.28808945175856593
2212	6	-0.3313929123383943
2212	7	-0.3933680246886624
2212	8	-0.4132181275264557
2212	9	-0.3642097363723451
2212	10	-0.37000976370326555
2212	11	-0.21522440551157018
2212	12	-0.2663091538300719
2212	13	-0.28085483966451363
2212	14	-0.2655916657015851
2212	15	-0.3217001815525933
2212	16	-0.3120236363461615
2212	17	-0.3024487911765519
2212	18	-0.4345470654952024
2212	19	-0.4316795528385313
2212	20	-0.37432163455120815
2212	21	-0.3440680414076951
2212	22	-0.33218409864922194
2212	23	-0.3633650512504403
2212	24	-0.47508051622202885
2212	25	-0.509943673396343
2213	1	-0.4214651434519716
2213	2	-0.4698131560967447
2213	3	-0.508343695568751
2213	4	-0.4699666874581254
2213	5	-0.4456574437882921
2213	6	-0.3643356696381801
2213	7	-0.46737871496262784
2213	8	-0.4597551027237401
2213	9	-0.4653138100080909
2213	10	-0.45857382439981365
2213	11	-0.3394638599977
2213	12	-0.44717381129829903
2213	13	-0.4085376261807738
2213	14	-0.5768421248824538
2213	15	-0.5253984974171857
2213	16	-0.42422329350845445
2213	17	-0.5656163404669527
2213	18	-0.4909133495038276
2213	19	-0.46866213816907987
2213	20	-0.4800693511377929
2213	21	-0.4514929046488021
2213	22	-0.5061984426904118
2213	23	-0.41971392436291666
2213	24	-0.46246275602802456
2213	25	-0.4195946502297545
2214	1	-0.39743632144610075
2214	2	-0.44367077611561
2214	3	-0.49677322581381844
2214	4	-0.6046425510950115
2214	5	-0.5372883326862166
2214	6	-0.5259106829881475
2214	7	-0.5819335010587068
2214	8	-0.5546556951546151
2214	9	-0.5383505557291954
2214	10	-0.5777715580613503
2214	11	-0.4851461192641082
2214	12	-0.41265085732576523
2214	13	-0.4830804978361769
2214	14	-0.6287198146738717
2214	15	-0.48789089574118805
2214	16	-0.560173413708732
2214	17	-0.5802865361234808
2214	18	-0.646031122407642
2214	19	-0.6833694407877857
2214	20	-0.6965334075381264
2214	21	-0.6777583773028795
2214	22	-0.6500811799722257
2214	23	-0.6007147441195713
2214	24	-0.6493548606414592
2214	25	-0.6262146344995898
2215	1	-0.47486409381374983
2215	2	-0.4235391562786859
2215	3	-0.5659852482058199
2215	4	-0.49823701315238367
2215	5	-0.5414203559154984
2215	6	-0.4916389556420455
2215	7	-0.4242144898059337
2215	8	-0.5096220353662275
2215	9	-0.6265317047600618
2215	10	-0.5720792744828955
2215	11	-0.4929588411330599
2215	12	-0.4367238393878381
2215	13	-0.47005783219491803
2215	14	-0.56515806686091
2215	15	-0.5482801134876354
2215	16	-0.6464200336223769
2215	17	-0.594148785704526
2215	18	-0.6308685682553445
2215	19	-0.7101910217210019
2215	20	-0.6703810234669803
2215	21	-0.6278144536670496
2215	22	-0.691251398455158
2215	23	-0.5863706100471759
2215	24	-0.6170096571504899
2215	25	-0.5772251707283473
2216	1	0.12179896799067791
2216	2	0.11066994724941637
2216	3	0.0583983313782781
2216	4	0.1858573455521082
2216	5	0.17484234843203694
2216	6	0.2320715198913561
2216	7	0.23978009547906964
2216	8	0.27851864584140884
2216	9	0.40840880643605715
2216	10	0.3770247167724534
2216	11	0.48946350831734975
2216	12	0.3578217766068832
2216	13	0.3556749241457453
2216	14	0.4342258409206181
2216	15	0.46234477512215477
2216	16	0.37973112280709387
2216	17	0.2001539466869957
2216	18	0.3059187772778742
2216	19	0.29456793730746256
2216	20	0.4379901360060572
2216	21	0.31977044733267945
2216	22	0.3411939020604393
2216	23	0.24288615527464685
2216	24	0.09025600909571807
2216	25	0.303800153405561
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	217358.0
3	12.0
4	1832.0
5	10557.0
6	14173.0
7	14591.0
8	12008.0
9	11551.0
10	11807.0
11	14127.0
12	24191.0
13	20741.0
14	8987.0
15	9150.0
16	9512.0
17	10441.0
18	11648.0
19	13636.0
20	17084.0
21	21538.0
22	26733.0
23	32446.0
24	38685.0
25	44514.0
26	51469.0
27	58666.0
28	68196.0
29	83566.0
30	107073.0
31	139897.0
32	185040.0
33	249165.0
34	351817.0
35	507207.0
36	781494.0
37	1493933.0
38	4507008.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.06109604181251	18.138467144233346	13.044598277195817	25.755838536758326
2	16.13108123364798	20.51245466975804	38.90032609958391	24.45613799701007
3	18.29759718548857	24.392810593779828	28.95538384516434	28.354208375567257
4	12.840229203136861	16.699883800953838	37.219441609515556	33.240445386393745
5	15.25280151370717	35.6414232661776	33.438999846486425	15.666775373628811
6	32.45283364326559	36.85615673900769	16.606127885500566	14.084881732226156
7	28.921848583366977	30.762760485198378	21.62534809000677	18.69004284142787
8	26.216364715005376	33.850388229791584	20.00019833217894	19.9330487230241
9	27.306613244362033	14.705505077382167	19.26631590660237	38.72156577165343
10	16.4793220938941	26.382879108749723	31.2648161726562	25.87298262469997
11	35.981648160714684	21.73055910009883	23.24508919308844	19.042703546098046
12	25.13314315179854	23.7742411300747	28.973795814135407	22.11881990399136
13	29.5698791540918	20.75897114579775	24.702883754049793	24.968265946060658
14	23.18137632543462	21.197493978735167	25.252349422340043	30.36878027349017
15	24.344146908110957	28.69434695094988	22.507653017041516	24.453853123897648
16	24.250862096182924	26.51340414858574	25.050833418110507	24.184900337120826
17	24.25685416036597	26.1762481624077	25.275020879301685	24.291876797924644
18	25.63161840204365	24.355171378750033	26.85582750457534	23.15738271463098
19	26.950237522218035	24.7463356549494	24.67798512052167	23.625441702310894
20	26.00916943390476	25.198281882255436	24.93891977403112	23.853628909808684
21	25.763588867953423	24.954676460796936	24.58909562617296	24.692639045076685
22	25.248248081349406	25.661526258308232	25.148888557836635	23.941337102505724
23	23.766766993982664	25.130825380600612	25.790298190348913	25.31210943506781
24	24.75524730014046	25.712357125741626	25.57410037749036	23.958295196627557
25	24.435396688595894	25.319010667137544	25.6286854926776	24.61690715158897
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4054.0
1	4054.0
2	4443.0
3	4832.0
4	4832.0
5	4832.0
6	8083.5
7	11335.0
8	11335.0
9	11335.0
10	16645.0
11	21955.0
12	21955.0
13	21955.0
14	32739.0
15	43523.0
16	43523.0
17	43523.0
18	70836.0
19	98149.0
20	98149.0
21	98149.0
22	148535.5
23	198922.0
24	198922.0
25	198922.0
26	281902.5
27	364883.0
28	364883.0
29	364883.0
30	452264.5
31	539646.0
32	539646.0
33	539646.0
34	631011.5
35	722377.0
36	722377.0
37	722377.0
38	775581.0
39	828785.0
40	828785.0
41	828785.0
42	900015.5
43	971246.0
44	971246.0
45	971246.0
46	1014425.0
47	1057604.0
48	1057604.0
49	1057604.0
50	1055258.0
51	1052912.0
52	1052912.0
53	1052912.0
54	966197.0
55	879482.0
56	879482.0
57	879482.0
58	818136.5
59	756791.0
60	756791.0
61	756791.0
62	673038.5
63	589286.0
64	589286.0
65	589286.0
66	499253.0
67	409220.0
68	409220.0
69	409220.0
70	327922.0
71	246624.0
72	246624.0
73	246624.0
74	204090.5
75	161557.0
76	161557.0
77	161557.0
78	137844.5
79	114132.0
80	114132.0
81	114132.0
82	87832.5
83	61533.0
84	61533.0
85	61533.0
86	45377.5
87	29222.0
88	29222.0
89	29222.0
90	20842.5
91	12463.0
92	12463.0
93	12463.0
94	6778.5
95	1094.0
96	1094.0
97	1094.0
98	660.0
99	226.0
100	226.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.13866482070667
2	0.15691821683488072
3	0.17037955192704565
4	0.18956957816684714
5	0.18013793076408433
6	0.06743736803453507
7	0.05432454647226437
8	0.05803839377519984
9	0.06708885450464085
10	0.0660977691540041
11	0.6859726462621434
12	0.6462856680454371
13	0.6443252794397819
14	0.6445866645872026
15	0.6459153724199244
16	0.6523846548185862
17	0.644553991443775
18	0.6548460316234642
19	0.6508381260296805
20	0.6561093931693309
21	0.643769836001513
22	0.6472114071092187
23	0.6322253253237663
24	0.6422559803560349
25	0.6361678846306948
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	9181853.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.78189329972093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.98569716984558	38.82228030402669
2	10.894223999192821	10.19304849426995
3	2.4887720333318946	3.492884031319866
4	1.014865711600589	1.8990935773457642
5	0.5547367422048941	1.2975817541632078
6	0.3363855624223571	0.9442052093285593
7	0.23793866616295237	0.7791854904619209
8	0.17726281838623892	0.663415220460227
9	0.13682975720319296	0.5761039591535846
>10	0.9377720998547513	8.483484969874604
>50	0.11297272287533269	3.696266629126284
>100	0.09536374166884605	9.138222108888488
>500	0.013391165077176616	4.321475465349804
>1k	0.0127110032795254	12.011474433973993
>5k	9.597626661902591E-4	3.062114045333406
>10k+	1.1704422758417794E-4	0.6191643069237759
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAAGGGCAGGGACTTAATCAA	14304	0.15578554786272444	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	11407	0.12423418235948669	No Hit
GTATCTGATCGTCTTCGAACCTCCG	10391	0.11316887778534464	No Hit
CTTTAGGACGTGAAATATGGCGAGG	10380	0.11304907625944349	No Hit
CTGTAGGACGTGGAATATGGCAAGA	10057	0.10953126781707352	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	9807	0.1068085058647748	No Hit
GTCCTACAGTGGACATTTCTAAATT	9761	0.10630751766555183	No Hit
GAATAGGACCGCGGTTCTATTTTGT	9713	0.10578474737071046	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	9210	0.10030655032268541	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0891047809194942E-5
2	0.0	0.0	0.0	0.0	1.0891047809194942E-5
3	0.0	0.0	0.0	0.0	1.0891047809194942E-5
4	0.0	0.0	0.0	2.1782095618389884E-5	1.0891047809194942E-5
5	0.0	0.0	0.0	3.267314342758482E-5	2.1782095618389884E-5
6	0.0	0.0	0.0	3.267314342758482E-5	3.267314342758482E-5
7	0.0	1.0891047809194942E-5	0.0	4.356419123677977E-5	4.356419123677977E-5
8	0.0	1.0891047809194942E-5	0.0	4.356419123677977E-5	5.4455239045974705E-5
9	0.0	1.0891047809194942E-5	0.0	9.801943028275447E-5	5.4455239045974705E-5
10	0.0	1.0891047809194942E-5	0.0	1.306925737103393E-4	6.534628685516964E-5
11	0.0	2.1782095618389884E-5	0.0	1.4158362151953425E-4	6.534628685516964E-5
12	0.0	2.1782095618389884E-5	0.0	1.4158362151953425E-4	1.306925737103393E-4
13	0.0	2.1782095618389884E-5	0.0	1.4158362151953425E-4	1.4158362151953425E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5280	0.0	15.9038925	1
GTATCAA	16505	0.0	13.060543	1
ACGCTTA	310	0.0	12.268348	17
AACCGCG	845	0.0	11.929118	7
GCGTTAT	685	0.0	11.747409	1
CTCGACG	335	0.0	11.637129	13
GGTAGCG	505	0.0	11.299261	11
CGTCGTA	1550	0.0	11.228184	10
AGAACCG	980	0.0	11.06211	5
CGGTCCA	1500	0.0	11.031844	10
CGGTTCT	1890	0.0	11.017231	12
AGGCCCG	1790	0.0	10.785323	10
ATCGCCA	1435	0.0	10.734089	16
CCGTCGT	1635	0.0	10.702563	9
CGCCAGT	1475	0.0	10.571572	18
CGGTAGC	550	0.0	10.5476885	10
ATACCGT	1685	0.0	10.497227	6
GCATCGC	1460	0.0	10.485103	14
CATCGCC	1485	0.0	10.436643	15
TCGACGC	365	0.0	10.419977	14
>>END_MODULE