FastQCFastQC Report
Thu 2 Feb 2017
SRR4050287_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050287_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9181853
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT146150.15917266373138408No Hit
GTACAAAGGGCAGGGACTTAATCAA137200.1494251759421546No Hit
GATTAAGAGGGACGGCCGGGGGCAT106700.11620748012411003No Hit
GTCCTAAAGTGTGTATTTCTCATTT104200.11348471817181129No Hit
CTTTAGGACGTGAAATATGGCGAGG102650.11179660576138607No Hit
GAATAGGACCGCGGTTCTATTTTGT99400.10825701522339772No Hit
CTGTAGGACGTGGAATATGGCAAGA99330.10818077788873334No Hit
TATCAACGCAGAGTACTTTTTTTTT95360.10385703190848297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53350.016.14741
GTATCAA161900.012.987851
CTCGACG3300.012.36860813
ACGCTTA3600.012.12857717
TAGATCG2750.011.4019265
CGTCGTA14700.011.23907910
AACCGCG8050.010.970917
TCGACGC3850.010.84809614
CGCTTAC4050.010.78095618
CCGTCGT16050.010.6489829
GTCGTAG15700.010.46273611
CCGTCCA6000.010.4448779
ATCGCCA13400.010.34212216
CGAACGA7350.010.33152116
TACCGTC17000.010.1107927
ACCGTCC6200.010.10794458
CATCGCC14000.010.0344915
ACGGTAT16000.010.0294559
ACCGTCG17800.010.0287898
ACACCGT6450.010.0151216
Produced by FastQC (version 0.11.5)