FastQCFastQC Report
Thu 2 Feb 2017
SRR4050286_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050286_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880117
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA237642.700095555477283No Hit
CTTCCGTACTCTGCGTTGATACCAC144941.646826501476508No Hit
GAGTACGGAAGCAGTGGTATCAACG119161.3539109004825494No Hit
TCCGTACTCTGCGTTGATACCACTG109481.2439255235383477No Hit
GCTTCCGTACTCTGCGTTGATACCA83050.9436245408280944No Hit
GCGTTGATACCACTGCTTCCGTACT58840.6685474772104163No Hit
GTATCAACGCAGAGTACGGAAGCAG56790.6452551194897951No Hit
GTACATGGAAGCAGTGGTATCAACG42920.4876624357897871No Hit
TATCAACGCAGAGTACGGAAGCAGT41420.4706192472137227No Hit
CCCATGTACTCTGCGTTGATACCAC38790.4407368565770233No Hit
TCCATGTACTCTGCGTTGATACCAC36170.41096808719749756No Hit
ACTCTGCGTTGATACCACTGCTTCC31930.36279267415582245No Hit
GTACATGGGAAGCAGTGGTATCAAC31790.36120197655538977No Hit
GGTATCAACGCAGAGTACGGAAGCA29310.3330239047762968No Hit
CAGTGGTATCAACGCAGAGTACGGA28440.3231388554021795No Hit
ATACCACTGCTTCCGTACTCTGCGT25990.29530164739460774No Hit
ACGCAGAGTACGGAAGCAGTGGTAT25950.29484716236591274No Hit
AAGCAGTGGTATCAACGCAGAGTAC19030.2162212524016693No Hit
GAAGCAGTGGTATCAACGCAGAGTA18730.21281261468645646No Hit
GTGGTATCAACGCAGAGTACGGAAG18700.2124717509149352No Hit
CATGTACTCTGCGTTGATACCACTG16840.19133819708061542No Hit
GTACTCTGCGTTGATACCACTGCTT15640.17770364621976395No Hit
GATACCACTGCTTCCGTACTCTGCG15580.17702191867672137No Hit
GAGTACATGGAAGCAGTGGTATCAA14620.1661142779880402No Hit
ATCAACGCAGAGTACGGAAGCAGTG14510.16486444415912885No Hit
GTTGATACCACTGCTTCCGTACTCT14260.16202391272978478No Hit
CCACTGCTTCCGTACTCTGCGTTGA14120.1604332151293521No Hit
GTATCAACGCAGAGTACATGGGAAG11890.13509567477960316No Hit
GTATCTGATCGTCTTCGAACCTCCG11890.13509567477960316No Hit
GAATAGGACCGCGGTTCTATTTTGT11870.13486843226525563No Hit
CTATTGGAGCTGGAATTACCGCGGC11220.1274830505489611No Hit
CTGCTTCCGTACTCTGCGTTGATAC11110.12623321672004972No Hit
CGGAAGCAGTGGTATCAACGCAGAG10950.12441527660526953No Hit
GAGTACATGGGAAGCAGTGGTATCA10770.1223700939761418No Hit
GTACGGGAAGCAGTGGTATCAACGC10750.12214285146179428No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG10470.11896145626092895No Hit
AAAAAGTACTCTGCGTTGATACCAC10460.11884783500375519No Hit
GATTAAGAGGGACGGCCGGGGGCAT10310.11714351614614876No Hit
GTCCTATTCCATTATTCCTAGCTGC9800.11134883203028688No Hit
GTATCAACGCAGAGTACTTTTTTTT9780.11112158951593935No Hit
GCAGAGTACGGAAGCAGTGGTATCA9610.10919002814398542No Hit
GAATAACGCCGCCGCATCGCCAGTC9600.10907640688681164No Hit
ATACAGGACTCTTTCGAGGCCCTGT9290.10555414791442502No Hit
CCCGTACTCTGCGTTGATACCACTG9240.10498604162855621No Hit
GCGTTGATACCACTGCTTCCCATGT9160.10407707157116611No Hit
GAACTACGACGGTATCTGATCGTCT9060.10294085899942848No Hit
CTCTTAATCATGGCCTCAGTTCCGA9000.1022591314563859No Hit
TTCCCGTACTCTGCGTTGATACCAC8970.10191826768486463No Hit
GTATCAACGCAGAGTACATGGAAGC8900.10112291888464829No Hit
CATGGAAGCAGTGGTATCAACGCAG8900.10112291888464829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACCA1301.8189894E-1213.9020729
TGTGCAA500.001484513413.3167210
ATCCCGT500.001484513413.3167210
TAATACT655.6397646E-513.105234
CGAACGA801.9850777E-613.06921316
ATACCGT957.252129E-813.0163436
CCGTCGT905.314341E-712.68259059
CGTCGTA905.314341E-712.682590510
AAGCTCG1051.9543222E-812.68259057
GTCGTAG1001.416247E-712.36552511
TACCGTC1001.416247E-712.3655257
TACGAAT1001.5107616E-712.3043562
CGCCAGT1700.012.30043618
ATACGAA854.052332E-612.2626251
GAACCGC1251.378794E-912.1752876
AGGCCCG2200.012.1061110
GCTCGTA1103.7389327E-812.106119
CTTGCAC550.003152840312.0462233
TACACAG951.0224048E-612.0150865
GAGTTAC1900.012.0089113
Produced by FastQC (version 0.11.5)