##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050286_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	880117
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.642105538240937	31.0	31.0	34.0	27.0	34.0
2	30.769480648595586	31.0	31.0	34.0	27.0	34.0
3	30.864604365101457	31.0	31.0	34.0	27.0	34.0
4	34.458837859057375	37.0	35.0	37.0	31.0	37.0
5	34.469160350271615	37.0	35.0	37.0	31.0	37.0
6	34.47801258241802	37.0	35.0	37.0	31.0	37.0
7	34.38383987583469	37.0	35.0	37.0	31.0	37.0
8	34.326185041307006	37.0	35.0	37.0	30.0	37.0
9	35.8663916274768	39.0	37.0	39.0	31.0	39.0
10	35.78080641551067	39.0	37.0	39.0	30.0	39.0
11	35.63000260192679	39.0	37.0	39.0	30.0	39.0
12	35.56768929585498	39.0	37.0	39.0	30.0	39.0
13	35.57746299639707	39.0	37.0	39.0	30.0	39.0
14	36.752730602863025	40.0	37.0	41.0	30.0	41.0
15	36.70717529601178	40.0	37.0	41.0	30.0	41.0
16	36.65305294636963	40.0	37.0	41.0	30.0	41.0
17	36.662866414351726	40.0	37.0	41.0	30.0	41.0
18	36.63186599054444	40.0	37.0	41.0	30.0	41.0
19	36.67524545032081	40.0	37.0	41.0	30.0	41.0
20	36.62944244912892	40.0	37.0	41.0	30.0	41.0
21	36.56236727616896	40.0	37.0	41.0	30.0	41.0
22	36.50392050147878	40.0	37.0	41.0	30.0	41.0
23	36.42974286373289	40.0	37.0	41.0	30.0	41.0
24	36.35184412981456	40.0	37.0	41.0	30.0	41.0
25	36.15260811914779	40.0	37.0	41.0	30.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.48749841572997
1101	2	0.30555959859374937
1101	3	0.2941140881787341
1101	4	0.3550357532838788
1101	5	0.468031315957127
1101	6	0.6143170313035782
1101	7	0.48252406824110494
1101	8	0.4664196836949728
1101	9	0.6613641703742985
1101	10	0.6280311495324185
1101	11	0.44326403912263856
1101	12	0.6084199993187909
1101	13	0.630348466929469
1101	14	0.8602919390113399
1101	15	0.807429148478505
1101	16	0.6580391357529365
1101	17	0.7699298431967208
1101	18	0.788581636923027
1101	19	0.7622605651818972
1101	20	0.7659724210307957
1101	21	0.7777340132105337
1101	22	0.6727911701615952
1101	23	0.8723275913606514
1101	24	0.807759720459039
1101	25	0.860469710406953
1102	1	0.7273397827227868
1102	2	0.6551080202554758
1102	3	0.6366068941685157
1102	4	0.5702696233578592
1102	5	0.7205982980816756
1102	6	0.6912476909025571
1102	7	0.5797332879353405
1102	8	0.6235378692951414
1102	9	0.5903747301287225
1102	10	0.47044125294683425
1102	11	0.48448055223726527
1102	12	0.5367672677111983
1102	13	0.7081935886749022
1102	14	0.755447907157766
1102	15	0.8005289134027009
1102	16	0.7079731129225664
1102	17	0.6502477997915079
1102	18	0.6228345375000544
1102	19	0.6463148960400957
1102	20	0.5727619404703574
1102	21	0.6860332862632532
1102	22	0.9168662407094033
1102	23	0.749524013040201
1102	24	0.7532963153740297
1102	25	0.9206120256669408
1103	1	0.5699401590529547
1103	2	0.4878331794561639
1103	3	0.41193149135779805
1103	4	0.48410778813836686
1103	5	0.46313503277442436
1103	6	0.3427349347008146
1103	7	0.3610296013016878
1103	8	0.41973283901155156
1103	9	0.44366392093054685
1103	10	0.5203317705636437
1103	11	0.6698819444049846
1103	12	0.6266118176301418
1103	13	0.6978740392113281
1103	14	0.7004616428225248
1103	15	0.6380191919080005
1103	16	0.5639862541203442
1103	17	0.6302219346819768
1103	18	0.7780577606812855
1103	19	0.6863301965274289
1103	20	0.7170744589957039
1103	21	0.7634210757899211
1103	22	0.7681103988431417
1103	23	0.7441953368248946
1103	24	0.7526902571096414
1103	25	0.805274943015057
1104	1	0.4226725975107435
1104	2	0.5912533089200949
1104	3	0.47524701352622145
1104	4	0.5917733865025667
1104	5	0.5619073575861435
1104	6	0.5117820878997961
1104	7	0.45481847423799593
1104	8	0.44288533252871076
1104	9	0.6045227148602379
1104	10	0.6120279464449965
1104	11	0.7273817859508327
1104	12	0.6198404518104965
1104	13	0.7538141407873269
1104	14	0.7643261052771635
1104	15	0.8398782035399179
1104	16	0.8457083613676204
1104	17	0.7716815316297314
1104	18	0.919834817071667
1104	19	0.8896806061658467
1104	20	0.8579360637642068
1104	21	0.8997621903701827
1104	22	0.9712227138995928
1104	23	1.0369618360353812
1104	24	1.1352498289803066
1104	25	1.05057739753871
1105	1	0.1392378581516347
1105	2	0.31506265301135983
1105	3	0.37299361617724713
1105	4	0.1363364150427202
1105	5	0.23915691345739987
1105	6	0.2794549072209449
1105	7	0.26125059060915845
1105	8	0.1981586705167473
1105	9	0.2470264417675665
1105	10	0.2766876993503615
1105	11	0.33556022461254287
1105	12	0.39043063757627294
1105	13	0.5120177849344003
1105	14	0.539306628311202
1105	15	0.45074378956071115
1105	16	0.3682851976613648
1105	17	0.42393209841618074
1105	18	0.5172339344753141
1105	19	0.42505589832033763
1105	20	0.527849340719925
1105	21	0.38246314009131055
1105	22	0.4549157083921216
1105	23	0.5094005139200632
1105	24	0.42921695671415705
1105	25	0.4292861757870554
1106	1	0.3358005847651242
1106	2	0.389957504352914
1106	3	0.24351573365367685
1106	4	0.3522646238897167
1106	5	0.18308367197806774
1106	6	0.3806296715429198
1106	7	0.27594964789705045
1106	8	0.4182783224384252
1106	9	0.36203731921499127
1106	10	0.34963548760271834
1106	11	0.40698879604111227
1106	12	0.46562643162993567
1106	13	0.47444345571032187
1106	14	0.5160361424518811
1106	15	0.46578367353460237
1106	16	0.6070705493655026
1106	17	0.5351467467120514
1106	18	0.5462368351279636
1106	19	0.4283263841796554
1106	20	0.5202169984429119
1106	21	0.1658641553197313
1106	22	0.4115944611114841
1106	23	0.20574569158503664
1106	24	0.3127485013116953
1106	25	0.33375318086319794
1107	1	0.08439636398590267
1107	2	0.30656464721005605
1107	3	0.27975761677056
1107	4	0.10754465635724841
1107	5	0.12665104210793743
1107	6	0.233584216997464
1107	7	0.22935062192302524
1107	8	0.06936598477609124
1107	9	0.10134332570849836
1107	10	0.27698126682349766
1107	11	0.33556022461254287
1107	12	0.36442107448707617
1107	13	0.4661398842817519
1107	14	0.4305815969973352
1107	15	0.5754265306774613
1107	16	0.48762655585900916
1107	17	0.5260801882704911
1107	18	0.43577823123185766
1107	19	0.6698401828809537
1107	20	0.5945473555857674
1107	21	0.6346303890859701
1107	22	0.5149183247478533
1107	23	0.49038043184477687
1107	24	0.48777285196104003
1107	25	0.6973246094346237
1108	1	0.16172920674469893
1108	2	0.2630623471392859
1108	3	0.22824427475164555
1108	4	0.3659452426340266
1108	5	0.3162274390390323
1108	6	0.16658862412607078
1108	7	0.1654623361995462
1108	8	0.14921503257895807
1108	9	0.14523416483221752
1108	10	0.16979977837210924
1108	11	0.1253691418099976
1108	12	0.0998742326611719
1108	13	-0.017505285569626494
1108	14	0.1559724481843645
1108	15	-0.0668682322383205
1108	16	0.1728611670987945
1108	17	0.11485708944787376
1108	18	0.1587780341978231
1108	19	0.2948929598545149
1108	20	0.2745113063996385
1108	21	0.10520395918182857
1108	22	0.09792142501806467
1108	23	0.1735406567048443
1108	24	0.17966691198806473
1108	25	0.11744138225840572
1109	1	-0.048074805899258166
1109	2	0.04273701841682964
1109	3	0.04163550379179526
1109	4	0.03190319632645355
1109	5	0.20101138646541727
1109	6	-0.01948324306540883
1109	7	-0.14319240271289146
1109	8	-0.1592239459354019
1109	9	-0.03695451463923405
1109	10	-0.14983976191840043
1109	11	0.16689402842341394
1109	12	0.2242316337672463
1109	13	-0.013537691582747868
1109	14	-0.08236188154247515
1109	15	0.0011057826181826158
1109	16	0.003241030260753064
1109	17	-0.012314186849799569
1109	18	-0.05238147316924113
1109	19	-0.03816318130073881
1109	20	-0.030844096425525436
1109	21	-0.1310317011174078
1109	22	-0.0019411775617683702
1109	23	-0.038518851211613025
1109	24	0.02493823818032581
1109	25	0.04533096088134414
1110	1	-0.2448432784139527
1110	2	-0.192282602957615
1110	3	-0.16899264998146535
1110	4	-0.04121961487621206
1110	5	-0.010796274366668968
1110	6	-0.2035956805697623
1110	7	-0.17866470488631592
1110	8	0.019683419688597326
1110	9	-0.016500574815864866
1110	10	-0.13779241313503832
1110	11	-0.05880597257055342
1110	12	-0.09819315254176075
1110	13	-0.11299115193555309
1110	14	-0.19247492394650578
1110	15	-0.07376025671354824
1110	16	-0.15803005425622985
1110	17	-0.08664064430069374
1110	18	-0.08780407701154047
1110	19	-0.11948716377473545
1110	20	-0.37936381979987743
1110	21	-0.1788642149015942
1110	22	0.058448921205382476
1110	23	-0.14538777588645502
1110	24	-0.10462909761520223
1110	25	-0.1803581538115182
1111	1	-0.014796330057144758
1111	2	0.018314311864649824
1111	3	-0.07509593636644141
1111	4	-0.0874972808721921
1111	5	-0.20127246484285877
1111	6	-0.13920936266231365
1111	7	-0.1893957578708907
1111	8	-0.22981356220275728
1111	9	-0.25191304966495665
1111	10	-0.23101843593852323
1111	11	-0.14800785050482546
1111	12	-0.2148933537490052
1111	13	-0.1787188514660656
1111	14	-0.22869222776944298
1111	15	-0.16698627951704026
1111	16	-0.22107499054060042
1111	17	-0.20199946388486723
1111	18	-0.2179180944495016
1111	19	-0.35489963862383433
1111	20	-0.3464999700346212
1111	21	-0.30227132422419345
1111	22	-0.3104310251393514
1111	23	-0.3586674539548653
1111	24	-0.3038242686413568
1111	25	-0.20919785870756158
1112	1	-0.04863324227740051
1112	2	0.018165817778619697
1112	3	-0.01369879095215154
1112	4	-0.06778076550349965
1112	5	0.05144248707601662
1112	6	0.037084521041244045
1112	7	0.009303685164127273
1112	8	-0.20141350747079656
1112	9	-0.21350018675730098
1112	10	-0.11284181770066937
1112	11	-0.17815214996939943
1112	12	-0.07343950243600261
1112	13	-6.138782600544346E-4
1112	14	-0.11643877393607482
1112	15	0.01409709923933633
1112	16	-0.02226294322885991
1112	17	-0.02603576642698613
1112	18	-0.04982862115488729
1112	19	0.10507268136073833
1112	20	0.1871424642814219
1112	21	-0.07091080884653422
1112	22	-0.0059975121326587555
1112	23	-0.2003794875836178
1112	24	-0.15420324259657292
1112	25	-0.13821050762223308
1113	1	-0.14147695251241288
1113	2	0.05456548896089686
1113	3	-0.14794650780856244
1113	4	-0.18764158101649286
1113	5	0.014072127966521464
1113	6	-0.2131750422617955
1113	7	-0.22413212218472012
1113	8	-0.26697660781650256
1113	9	-0.08702472984705878
1113	10	-0.2549316119643805
1113	11	-0.08800879895647995
1113	12	-0.10830093729743595
1113	13	-0.14935885309198227
1113	14	-0.16992345350771387
1113	15	-0.11552464777371796
1113	16	-0.05429789700294663
1113	17	-0.1643554177901123
1113	18	0.012761606652738067
1113	19	-0.14075009489682344
1113	20	-0.09612499289907817
1113	21	0.003906483362058566
1113	22	-0.07857388905686236
1113	23	-0.17917975334040648
1113	24	0.029155427718620786
1113	25	-0.10292791581790084
1114	1	-0.3266171505962454
1114	2	-0.22211924283865514
1114	3	-0.1827145079210304
1114	4	-0.21375237581204232
1114	5	-0.23253169734293522
1114	6	-0.46318382880502895
1114	7	-0.27586166535476764
1114	8	-0.37573151949417394
1114	9	-0.4935745706348982
1114	10	-0.11730903968627615
1114	11	-0.10215724233248125
1114	12	-0.17897989106616308
1114	13	-0.33501921453522954
1114	14	-0.362690371118056
1114	15	-0.3251040886683967
1114	16	-0.2920730036715824
1114	17	-0.2958667735185898
1114	18	-0.33062915552716987
1114	19	-0.421629873897686
1114	20	-0.24680507134534224
1114	21	-0.49150834691707246
1114	22	-0.5193589139755019
1114	23	-0.3246708508148046
1114	24	-0.3829657844025931
1114	25	-0.5172717719154107
1115	1	-0.4227143827431341
1115	2	-0.3501980298965819
1115	3	-0.4178084380731768
1115	4	-0.30290156339057006
1115	5	-0.2499913253586712
1115	6	-0.42554482510137603
1115	7	-0.3516534201758148
1115	8	-0.4734213686236828
1115	9	-0.478998155377532
1115	10	-0.42846560965944747
1115	11	-0.4228290371324235
1115	12	-0.25721739493872775
1115	13	-0.293566025487074
1115	14	-0.36978786947950937
1115	15	-0.5603396885619105
1115	16	-0.532335544168113
1115	17	-0.41338260238777025
1115	18	-0.4990635803402341
1115	19	-0.6007595857787962
1115	20	-0.4164693763307099
1115	21	-0.35756505529068505
1115	22	-0.4627828692389997
1115	23	-0.506705570799113
1115	24	-0.5268882303384643
1115	25	-0.3976604763222724
1116	1	-0.05843989770755087
1116	2	0.12574340710451537
1116	3	0.04029296697444806
1116	4	0.08959783157911971
1116	5	0.2397270945185852
1116	6	-0.019159246662887597
1116	7	0.06660550918615371
1116	8	0.07534013924958316
1116	9	0.04025066259983845
1116	10	0.12416809220128755
1116	11	0.1614177444014615
1116	12	-0.21788845717519223
1116	13	-0.0710880180929152
1116	14	-0.1908504010837362
1116	15	-0.2924824402987909
1116	16	0.056563576127658166
1116	17	-0.007467275149991792
1116	18	0.10882070244797859
1116	19	0.224002976716406
1116	20	0.054206821284978446
1116	21	0.07967116625075477
1116	22	0.13782137404024297
1116	23	0.01583992452887628
1116	24	-0.17056472899362518
1116	25	-0.004852171568018093
1201	1	0.6587167246724128
1201	2	0.6405432607793777
1201	3	0.6722346548504738
1201	4	0.5839102201813304
1201	5	0.7332416997909661
1201	6	0.8170013529588473
1201	7	0.7950225513947302
1201	8	0.7217275428345502
1201	9	0.8607941670868797
1201	10	0.8630088546486547
1201	11	0.8436723190078226
1201	12	0.8312121880563978
1201	13	0.8437670966711295
1201	14	1.027658561330938
1201	15	1.0505371501464893
1201	16	1.0104439164114325
1201	17	1.0024855583428973
1201	18	0.9727791865818176
1201	19	0.950610412835367
1201	20	1.0264011535208724
1201	21	1.0558894397697998
1201	22	1.06893959385485
1201	23	1.1207815112226243
1201	24	1.1199840681047846
1201	25	1.1799942259537985
1202	1	0.4746871393869725
1202	2	0.48781464721005463
1202	3	0.35076363281334366
1202	4	0.4602268087636645
1202	5	0.30020552071756157
1202	6	0.27562967154291584
1202	7	0.3149622529390683
1202	8	0.4177951291611137
1202	9	0.5769532856015402
1202	10	0.5492573363422153
1202	11	0.7892366952007777
1202	12	0.6299541627223704
1202	13	0.5615442960464563
1202	14	0.645153789510708
1202	15	0.6291030012656975
1202	16	0.4834571039873481
1202	17	0.47987363746835143
1202	18	0.5393460788254458
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2210	8	-0.010930690025617196
2210	9	0.10086761643454878
2210	10	0.04655303314442705
2210	11	-0.021486755253228296
2210	12	1.2786978908962965E-4
2210	13	-0.00527370632227786
2210	14	0.08308312231765314
2210	15	0.09245673201974114
2210	16	-0.034870965495478856
2210	17	-0.06878901551230143
2210	18	0.06223395880964944
2210	19	0.053379116682052086
2210	20	0.1785343522300593
2210	21	-0.00208407095063734
2210	22	-0.07605654559992558
2210	23	-0.07068767370738982
2210	24	-0.10041544883212339
2210	25	-0.11056129660564551
2211	1	-0.31383889855857205
2211	2	-0.21633515036079487
2211	3	-0.2868197203659868
2211	4	-0.22755897356546484
2211	5	-0.46329670203388673
2211	6	-0.23148908689162795
2211	7	-0.2817542271752487
2211	8	-0.5177897323138367
2211	9	-0.3728383314501329
2211	10	-0.4670282872189446
2211	11	-0.3446017187072954
2211	12	-0.4561655483199445
2211	13	-0.4743332938558993
2211	14	-0.5715347077505442
2211	15	-0.5265471535330661
2211	16	-0.3432099595992213
2211	17	-0.4365796847512229
2211	18	-0.41505774748240754
2211	19	-0.5802585396684279
2211	20	-0.3649140985302921
2211	21	-0.42945683947494473
2211	22	-0.448108642802147
2211	23	-0.5666236934159272
2211	24	-0.6103611960085331
2211	25	-0.731656497178065
2212	1	-0.45889662020382005
2212	2	-0.514766874529073
2212	3	-0.3826682725575026
2212	4	-0.33801487921587636
2212	5	-0.45558092429522645
2212	6	-0.41513119802230136
2212	7	-0.4900169670097796
2212	8	-0.6241246278721349
2212	9	-0.6267631466235173
2212	10	-0.43886606519230753
2212	11	-0.5007169493005037
2212	12	-0.7615979472520564
2212	13	-0.6228546809356388
2212	14	-0.6605127705915947
2212	15	-0.554328904105148
2212	16	-0.6012136121251856
2212	17	-0.5646436259587588
2212	18	-0.6852383201515408
2212	19	-0.7371200443917729
2212	20	-0.6320151444142326
2212	21	-0.512664571388342
2212	22	-0.48017184323634154
2212	23	-0.5925617618311136
2212	24	-0.7545690452721558
2212	25	-0.6552569123045089
2213	1	-0.4095101604523492
2213	2	-0.49737855732025693
2213	3	-0.5294784606929461
2213	4	-0.625011953630036
2213	5	-0.5137160805678533
2213	6	-0.46872409261430903
2213	7	-0.6666514751637678
2213	8	-0.5611625265194107
2213	9	-0.5873107344635144
2213	10	-0.6266312869285429
2213	11	-0.47789747025754536
2213	12	-0.5272292836075252
2213	13	-0.5500264215143815
2213	14	-0.6886873752030169
2213	15	-0.6711174400087501
2213	16	-0.6057785036467749
2213	17	-0.5409972525265516
2213	18	-0.8496739862292273
2213	19	-0.6276201276695872
2213	20	-0.7675601977120365
2213	21	-0.6885405272728207
2213	22	-0.8022229939184911
2213	23	-0.6777205690031423
2213	24	-0.6201410009249102
2213	25	-0.5754102340030869
2214	1	-0.7934177440758461
2214	2	-0.8242473473991083
2214	3	-0.8273737542169428
2214	4	-0.8768054569728179
2214	5	-0.7753395086149268
2214	6	-0.9030091424732518
2214	7	-0.7334304060113084
2214	8	-0.7682580657017368
2214	9	-0.8593912522135838
2214	10	-0.795661008354152
2214	11	-1.0417982659534957
2214	12	-0.9732010616315208
2214	13	-0.9711495362034199
2214	14	-1.0462010543136202
2214	15	-1.0302001539586598
2214	16	-0.9548297201762779
2214	17	-1.1296376198647664
2214	18	-1.108089310424596
2214	19	-1.0898407317233065
2214	20	-1.0438665595085723
2214	21	-1.2403083514188111
2214	22	-1.1685672639559002
2214	23	-1.3589173811912403
2214	24	-1.3081679407368227
2214	25	-1.2686457409696885
2215	1	-0.7751147274675532
2215	2	-0.7579937360234759
2215	3	-0.7783619395713011
2215	4	-0.8524316977527135
2215	5	-0.9960651731887395
2215	6	-0.909549969175643
2215	7	-0.7727843127530818
2215	8	-0.8720296330791086
2215	9	-0.7701055379278685
2215	10	-0.8282259324143837
2215	11	-0.5753679191000316
2215	12	-0.9468158267105267
2215	13	-0.890428229490702
2215	14	-1.0359399054523095
2215	15	-0.9810854453704394
2215	16	-0.9996531461007123
2215	17	-0.9849687366070228
2215	18	-1.086094217053386
2215	19	-1.2104546252300992
2215	20	-1.1154545156717148
2215	21	-1.0116679233800099
2215	22	-1.1945732035591732
2215	23	-1.4919531963533714
2215	24	-1.3522771616481037
2215	25	-1.2757292827809437
2216	1	-0.4514492242493908
2216	2	-0.32328160947443507
2216	3	-0.32176310515457374
2216	4	-0.31871704150371727
2216	5	-0.37205383757120813
2216	6	-0.36835428567633244
2216	7	-0.22093346898606825
2216	8	-0.27304711682818805
2216	9	-0.2868167678743916
2216	10	-0.026411112855640795
2216	11	-0.32219378608264293
2216	12	-0.45480519556640076
2216	13	-0.40743966117279484
2216	14	-0.5025734832165654
2216	15	-0.3520467313546263
2216	16	-0.43646268210891037
2216	17	-0.6040167368632012
2216	18	-0.4479533864152003
2216	19	-0.4616354187233256
2216	20	-0.38427116045701126
2216	21	-0.6360915360477222
2216	22	-0.5725231859473396
2216	23	-0.6303582045188563
2216	24	-0.7800246079320061
2216	25	-0.7846272622231325
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	19463.0
3	2.0
4	173.0
5	1140.0
6	1680.0
7	1791.0
8	1519.0
9	1348.0
10	1388.0
11	1950.0
12	2134.0
13	1863.0
14	944.0
15	922.0
16	977.0
17	1064.0
18	1201.0
19	1561.0
20	2000.0
21	2520.0
22	3122.0
23	3608.0
24	4268.0
25	4750.0
26	5501.0
27	6044.0
28	7093.0
29	8606.0
30	10779.0
31	14132.0
32	18246.0
33	24680.0
34	34906.0
35	49823.0
36	77374.0
37	146464.0
38	415081.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.95529053978689	16.21887398902352	12.700687224990496	24.125148246199092
2	14.335913083995319	21.19929994431375	38.63455047560602	25.83023649608492
3	19.79433044877696	27.49921009981156	27.79368943624994	24.912770015161538
4	13.033030438746257	15.387605799025614	34.937151457145	36.64221230508313
5	16.44761218491502	34.52006155650674	30.74756604026632	18.284760218311924
6	34.96386388673827	33.64882087242821	15.193084058386228	16.194231182447297
7	27.948439823721227	31.698797975250027	21.39256136329527	18.96020083773348
8	26.94552429638439	32.58229457198636	21.45577054052733	19.016410591101916
9	28.722533469150175	16.78232319557625	17.035160797438884	37.45998253783469
10	15.68224910993193	25.505849668299025	29.61482673796305	29.19707448380599
11	34.95338321795847	23.637995392627058	19.910139078639737	21.498482310774737
12	26.25867470402607	23.47500017138039	26.666674283572846	23.599650841020694
13	30.778113848923326	19.797801424596447	25.935491092845492	23.48859363363473
14	28.17658419477118	19.71753290138664	23.53241484699699	28.57346805684519
15	29.41920273590498	23.23143366584082	23.903530564760146	23.445833033494047
16	26.793977516182228	23.999716782466095	26.477756003526515	22.728549697825162
17	24.510744435917115	26.86463015751197	25.491311259730292	23.133314146840625
18	26.528708065667377	21.605367594575302	30.393604568165593	21.47231977159172
19	26.138027609176866	28.152519799978528	21.76752643614093	23.94192615470368
20	23.846794300013364	25.997480530070888	26.02249249355571	24.133232676360038
21	25.54170107443614	27.977485611555984	21.933025633645485	24.547787680362386
22	23.889150286191978	26.460424194528322	21.684581264360993	27.96584425491871
23	25.107643179705292	23.63592963733671	22.073533140394115	29.182894042563884
24	22.381151944859024	26.492630626947683	22.38332159060155	28.742895837591735
25	26.152649718275438	25.960569981203136	22.441239816462215	25.445540484059208
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	176.0
1	176.0
2	284.0
3	392.0
4	392.0
5	392.0
6	825.5
7	1259.0
8	1259.0
9	1259.0
10	1488.5
11	1718.0
12	1718.0
13	1718.0
14	2352.0
15	2986.0
16	2986.0
17	2986.0
18	4658.5
19	6331.0
20	6331.0
21	6331.0
22	9168.5
23	12006.0
24	12006.0
25	12006.0
26	17238.0
27	22470.0
28	22470.0
29	22470.0
30	27487.0
31	32504.0
32	32504.0
33	32504.0
34	39804.0
35	47104.0
36	47104.0
37	47104.0
38	52090.5
39	57077.0
40	57077.0
41	57077.0
42	65645.0
43	74213.0
44	74213.0
45	74213.0
46	99192.0
47	124171.0
48	124171.0
49	124171.0
50	167370.0
51	210569.0
52	210569.0
53	210569.0
54	150125.0
55	89681.0
56	89681.0
57	89681.0
58	77106.5
59	64532.0
60	64532.0
61	64532.0
62	57592.5
63	50653.0
64	50653.0
65	50653.0
66	42043.5
67	33434.0
68	33434.0
69	33434.0
70	26289.5
71	19145.0
72	19145.0
73	19145.0
74	15800.5
75	12456.0
76	12456.0
77	12456.0
78	10975.5
79	9495.0
80	9495.0
81	9495.0
82	7058.5
83	4622.0
84	4622.0
85	4622.0
86	3430.5
87	2239.0
88	2239.0
89	2239.0
90	1516.0
91	793.0
92	793.0
93	793.0
94	430.5
95	68.0
96	68.0
97	68.0
98	45.5
99	23.0
100	23.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17168171958955458
2	0.021247175091493518
3	0.02942790560800439
4	0.04351694149755089
5	0.031245845722784587
6	0.05976478127339888
7	0.06828637556143104
8	0.07089966447642757
9	0.05692424984405482
10	0.04647109418406871
11	0.6171906689678759
12	0.5534491436933954
13	0.52731625454343
14	0.5165222351119226
15	0.5281116033436464
16	0.5073189132808479
17	0.5114092785391033
18	0.5103866872245394
19	0.5239076168282172
20	0.515386022540185
21	0.5212943279132206
22	0.5091368533956281
23	0.5157268863117063
24	0.49970628905020587
25	0.5043647605943301
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	880117.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.50930396569973
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.72068773300278	41.26337433820154
2	7.286244616276591	6.777563312537023
3	1.4851709812580804	2.0722280582510586
4	0.604817514147651	1.1251856643708797
5	0.34293761263433375	0.7974894833640808
6	0.20356807764550447	0.5680685760556758
7	0.15988742434408773	0.5205376973378396
8	0.12572829929180893	0.4678028551082569
9	0.09137053404139679	0.38246219471156745
>10	0.690181849114057	6.513697319471968
>50	0.12242194166249516	4.083449632961524
>100	0.1407851386133044	13.656472526316996
>500	0.016894216633567643	5.529319824784509
>1k	0.007590155299139085	7.0196033849409245
>5k	7.345311579812019E-4	2.2624706787795255
>10k+	9.79374877308269E-4	6.9602744528066305
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	23764	2.700095555477283	No Hit
CTTCCGTACTCTGCGTTGATACCAC	14494	1.646826501476508	No Hit
GAGTACGGAAGCAGTGGTATCAACG	11916	1.3539109004825494	No Hit
TCCGTACTCTGCGTTGATACCACTG	10948	1.2439255235383477	No Hit
GCTTCCGTACTCTGCGTTGATACCA	8305	0.9436245408280944	No Hit
GCGTTGATACCACTGCTTCCGTACT	5884	0.6685474772104163	No Hit
GTATCAACGCAGAGTACGGAAGCAG	5679	0.6452551194897951	No Hit
GTACATGGAAGCAGTGGTATCAACG	4292	0.4876624357897871	No Hit
TATCAACGCAGAGTACGGAAGCAGT	4142	0.4706192472137227	No Hit
CCCATGTACTCTGCGTTGATACCAC	3879	0.4407368565770233	No Hit
TCCATGTACTCTGCGTTGATACCAC	3617	0.41096808719749756	No Hit
ACTCTGCGTTGATACCACTGCTTCC	3193	0.36279267415582245	No Hit
GTACATGGGAAGCAGTGGTATCAAC	3179	0.36120197655538977	No Hit
GGTATCAACGCAGAGTACGGAAGCA	2931	0.3330239047762968	No Hit
CAGTGGTATCAACGCAGAGTACGGA	2844	0.3231388554021795	No Hit
ATACCACTGCTTCCGTACTCTGCGT	2599	0.29530164739460774	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	2595	0.29484716236591274	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1903	0.2162212524016693	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	1873	0.21281261468645646	No Hit
GTGGTATCAACGCAGAGTACGGAAG	1870	0.2124717509149352	No Hit
CATGTACTCTGCGTTGATACCACTG	1684	0.19133819708061542	No Hit
GTACTCTGCGTTGATACCACTGCTT	1564	0.17770364621976395	No Hit
GATACCACTGCTTCCGTACTCTGCG	1558	0.17702191867672137	No Hit
GAGTACATGGAAGCAGTGGTATCAA	1462	0.1661142779880402	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	1451	0.16486444415912885	No Hit
GTTGATACCACTGCTTCCGTACTCT	1426	0.16202391272978478	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	1412	0.1604332151293521	No Hit
GTATCAACGCAGAGTACATGGGAAG	1189	0.13509567477960316	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1189	0.13509567477960316	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1187	0.13486843226525563	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1122	0.1274830505489611	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	1111	0.12623321672004972	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	1095	0.12441527660526953	No Hit
GAGTACATGGGAAGCAGTGGTATCA	1077	0.1223700939761418	No Hit
GTACGGGAAGCAGTGGTATCAACGC	1075	0.12214285146179428	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1047	0.11896145626092895	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1046	0.11884783500375519	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1031	0.11714351614614876	No Hit
GTCCTATTCCATTATTCCTAGCTGC	980	0.11134883203028688	No Hit
GTATCAACGCAGAGTACTTTTTTTT	978	0.11112158951593935	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	961	0.10919002814398542	No Hit
GAATAACGCCGCCGCATCGCCAGTC	960	0.10907640688681164	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	929	0.10555414791442502	No Hit
CCCGTACTCTGCGTTGATACCACTG	924	0.10498604162855621	No Hit
GCGTTGATACCACTGCTTCCCATGT	916	0.10407707157116611	No Hit
GAACTACGACGGTATCTGATCGTCT	906	0.10294085899942848	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	900	0.1022591314563859	No Hit
TTCCCGTACTCTGCGTTGATACCAC	897	0.10191826768486463	No Hit
GTATCAACGCAGAGTACATGGAAGC	890	0.10112291888464829	No Hit
CATGGAAGCAGTGGTATCAACGCAG	890	0.10112291888464829	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.681062858688106E-4	0.0
11	0.0	0.0	0.0	6.817275430425727E-4	0.0
12	0.0	0.0	0.0	7.953488002163349E-4	0.0
13	0.0	0.0	0.0	9.08970057390097E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGACCA	130	1.8189894E-12	13.902072	9
TGTGCAA	50	0.0014845134	13.31672	10
ATCCCGT	50	0.0014845134	13.31672	10
TAATACT	65	5.6397646E-5	13.10523	4
CGAACGA	80	1.9850777E-6	13.069213	16
ATACCGT	95	7.252129E-8	13.016343	6
CCGTCGT	90	5.314341E-7	12.6825905	9
CGTCGTA	90	5.314341E-7	12.6825905	10
AAGCTCG	105	1.9543222E-8	12.6825905	7
GTCGTAG	100	1.416247E-7	12.365525	11
TACCGTC	100	1.416247E-7	12.365525	7
TACGAAT	100	1.5107616E-7	12.304356	2
CGCCAGT	170	0.0	12.300436	18
ATACGAA	85	4.052332E-6	12.262625	1
GAACCGC	125	1.378794E-9	12.175287	6
AGGCCCG	220	0.0	12.10611	10
GCTCGTA	110	3.7389327E-8	12.10611	9
CTTGCAC	55	0.0031528403	12.046223	3
TACACAG	95	1.0224048E-6	12.015086	5
GAGTTAC	190	0.0	12.00891	13
>>END_MODULE