FastQCFastQC Report
Thu 2 Feb 2017
SRR4050286_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050286_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences880117
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA205332.3329852735488577No Hit
CTTCCGTACTCTGCGTTGATACCAC140621.5977421183774427No Hit
TCCGTACTCTGCGTTGATACCACTG116261.3209607359021585No Hit
GAGTACGGAAGCAGTGGTATCAACG111981.2723308378317884No Hit
GCTTCCGTACTCTGCGTTGATACCA77780.8837461382975219No Hit
GCGTTGATACCACTGCTTCCGTACT56480.6417328605174084No Hit
GTATCAACGCAGAGTACGGAAGCAG53050.6027607693068081No Hit
TATCAACGCAGAGTACGGAAGCAGT46560.5290205734010365No Hit
CCCATGTACTCTGCGTTGATACCAC41330.46959665589915883No Hit
TCCATGTACTCTGCGTTGATACCAC39900.4533488161233109No Hit
ACTCTGCGTTGATACCACTGCTTCC34720.3944930049073021No Hit
GTACATGGAAGCAGTGGTATCAACG33410.37960862021753927No Hit
CAGTGGTATCAACGCAGAGTACGGA30320.34449965175084674No Hit
ACGCAGAGTACGGAAGCAGTGGTAT30040.34131825654998144No Hit
GGTATCAACGCAGAGTACGGAAGCA29510.33529632991977204No Hit
ATACCACTGCTTCCGTACTCTGCGT27710.3148445036284948No Hit
GTACATGGGAAGCAGTGGTATCAAC26640.3026870291109023No Hit
AAGCAGTGGTATCAACGCAGAGTAC22110.2512165996111881No Hit
GAAGCAGTGGTATCAACGCAGAGTA19760.224515604175354No Hit
GTGGTATCAACGCAGAGTACGGAAG19340.21974351137405596No Hit
CATGTACTCTGCGTTGATACCACTG18980.21565314611580053No Hit
ATCAACGCAGAGTACGGAAGCAGTG17610.20008703388299512No Hit
GATACCACTGCTTCCGTACTCTGCG16280.18497540667888474No Hit
GTATCAACGCAGAGTACTTTTTTTT15830.17986245010606544No Hit
GTTGATACCACTGCTTCCGTACTCT13950.15850165375739816No Hit
CCACTGCTTCCGTACTCTGCGTTGA13800.15679733489979172No Hit
CGGAAGCAGTGGTATCAACGCAGAG12830.14577607295393682No Hit
GTACTCTGCGTTGATACCACTGCTT12730.14463986038219917No Hit
GAGTACATGGAAGCAGTGGTATCAA12500.14202657146720266No Hit
AAAAAGTACTCTGCGTTGATACCAC12230.1389587975235111No Hit
CTGCTTCCGTACTCTGCGTTGATAC11860.1347548110080819No Hit
GATTAAGAGGGACGGCCGGGGGCAT11580.13157341580721654No Hit
CTATTGGAGCTGGAATTACCGCGGC11050.12555148917700715No Hit
GAATAGGACCGCGGTTCTATTTTGT10780.12248371523331557No Hit
TATCAACGCAGAGTACTTTTTTTTT10750.12214285146179428No Hit
GTATCAACGCAGAGTACATGGGAAG10650.12100663889005665No Hit
GCAGAGTACGGAAGCAGTGGTATCA10560.1199840475754928No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG10370.11782524368919131No Hit
CATGGAAGCAGTGGTATCAACGCAG10220.11612092483158488No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9800.11134883203028688No Hit
GCGTTGATACCACTGCTTCCCATGT9590.10896278562963788No Hit
GTACGGGAAGCAGTGGTATCAACGC9510.10805381557224779No Hit
GAATAACGCCGCCGCATCGCCAGTC9500.10794019431507403No Hit
GAGTACATGGGAAGCAGTGGTATCA9390.10669036048616264No Hit
CTTTAATATACGCTATTGGAGCTGG9370.10646311797181511No Hit
GTATCTGATCGTCTTCGAACCTCCG9360.10634949671464135No Hit
CCCGTACTCTGCGTTGATACCACTG9320.1058950116859463No Hit
GCGTTGATACCACTGCTTCCATGTA9110.1035089652852973No Hit
GTCCTATTCCATTATTCCTAGCTGC8930.10146378265616957No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCCG453.530809E-516.8918195
TACCGTC1450.015.7170367
TAAGGCC551.1114704E-515.5685154
ATACCGT1600.015.43228156
ACCGTCG1600.015.4287748
TCAGATA1650.014.9919032
TCCTACA400.005225049314.2711372
CTACTAG551.9062977E-413.8623591
AGATACC1700.013.431664
CCGTCGT1850.013.3430469
GTAGCAC500.001481995113.319733
CGAACGT957.230847E-813.0192844
GAACGTC957.355629E-813.0022565
CAGATAC2050.012.9948573
CCGACCA1550.012.8629639
CGTCGTA1700.012.84494610
TAGGAGT604.038068E-412.6854554
ATCCTAC604.1005673E-412.6623866
GTCGTAG1750.012.47794811
CGCCAGT1600.012.459578518
Produced by FastQC (version 0.11.5)