FastQCFastQC Report
Thu 2 Feb 2017
SRR4050285_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050285_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7110217
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA172640.24280552900143557No Hit
GTATCTGATCGTCTTCGAACCTCCG121680.17113401742872264No Hit
GAATAGGACCGCGGTTCTATTTTGT118800.16708350814046885No Hit
GATTAAGAGGGACGGCCGGGGGCAT110330.15517107283786136No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG109140.15349742490278426No Hit
CTATTGGAGCTGGAATTACCGCGGC99660.14016449849561552No Hit
GAACTACGACGGTATCTGATCGTCT99610.1400941771538056No Hit
GTCCTATTCCATTATTCCTAGCTGC99430.1398410203232897No Hit
GTCTTGCGCCGGTCCAAGAATTTCA93020.13082582430325262No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC91520.12871618404895377No Hit
GTATCAACGCAGAGTACGGGCGGTG88590.12459535341889003No Hit
TCGTAGTTCCGACCATAAACGATGC87280.12275293426346903No Hit
GGGTAGGCACACGCTGAGCCAGTCA87200.1226404201165731No Hit
ATCAGATACCGTCGTAGTTCCGACC86430.12155747145269968No Hit
CTCTTAATCATGGCCTCAGTTCCGA86010.120966772181496No Hit
CTTATGACCCGCACTTACTGGGAAT85460.12019323742158641No Hit
ATACAGGACTCTTTCGAGGCCCTGT84940.11946189546676284No Hit
GAATAACGCCGCCGCATCGCCAGTC82350.11581924996100681No Hit
GACTTAATCAACGCAAGCTTATGAC82300.11574892861919686No Hit
CTTTAATATACGCTATTGGAGCTGG81690.11489100824911531No Hit
GAATAATTGCAATCCCCGATCCCCA79280.11150151957387516No Hit
GTGCATGGCCGTTCTTAGTTGGTGG77920.10958877907664422No Hit
ATATTAAAGTTGCTGCAGTTAAAAA75440.10610084052287011No Hit
GTCCTGTATTGTTATTTTTCGTCAC75110.10563671966692437No Hit
GCGCAAGACGGACCAGAGCGAAAGC73910.10394900746348529No Hit
GCTTTGAACACTCTAATTTTTTCAA73550.10344269380245356No Hit
CTCTAGATAACCTCGGGCCGATCGC72830.10243006648039013No Hit
GAATAATGGAATAGGACCGCGGTTC71130.10003914085885142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40200.015.4948891
GTATCAA139750.013.5816081
AACCGCG10100.011.7697047
CCGTCCA3850.011.6100889
CGGTTCT22200.011.47898212
AGGCCCG21400.011.46579910
CGTCGTA14400.011.29359110
CTCGACG2450.011.25488713
CGACCAT16650.011.19541610
GGTTCTA22500.011.15656913
ACCGTCC4350.011.1501168
ACTCTAA14450.010.99124910
AGAACCG11550.010.8684045
CGAGCCG22500.010.8605215
CCGACCA17800.010.8461229
TGCGTTA1951.8189894E-1210.7274713
CCGTCGT16050.010.7251159
GGTAGCG3750.010.65163911
ACGCTTA2600.010.604815517
GCGCGCT9300.010.53062815
Produced by FastQC (version 0.11.5)