##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050285_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7110217
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.444950976882986	31.0	31.0	34.0	27.0	34.0
2	30.573796974128918	31.0	31.0	34.0	27.0	34.0
3	30.678273954226714	31.0	31.0	34.0	27.0	34.0
4	34.23416261416494	37.0	35.0	37.0	30.0	37.0
5	34.21821106163145	37.0	35.0	37.0	30.0	37.0
6	34.26556109328309	37.0	35.0	37.0	30.0	37.0
7	34.19910250277875	37.0	35.0	37.0	30.0	37.0
8	34.151484124886764	37.0	35.0	37.0	30.0	37.0
9	35.72918421477151	39.0	37.0	39.0	30.0	39.0
10	35.60886200800904	39.0	37.0	39.0	30.0	39.0
11	35.506502825441196	39.0	37.0	39.0	30.0	39.0
12	35.42392348925497	39.0	37.0	39.0	30.0	39.0
13	35.42963962984533	39.0	37.0	39.0	30.0	39.0
14	36.57807701227684	40.0	37.0	41.0	30.0	41.0
15	36.51966515227313	40.0	37.0	41.0	30.0	41.0
16	36.52557622362299	40.0	37.0	41.0	30.0	41.0
17	36.49034959129939	40.0	37.0	41.0	30.0	41.0
18	36.449987813311466	40.0	37.0	41.0	30.0	41.0
19	36.460852882549155	40.0	37.0	41.0	30.0	41.0
20	36.41570475275227	40.0	37.0	41.0	30.0	41.0
21	36.35921224345192	40.0	37.0	41.0	30.0	41.0
22	36.27128918850156	40.0	37.0	41.0	30.0	41.0
23	36.187713539544575	40.0	37.0	41.0	29.0	41.0
24	36.11176480267761	40.0	37.0	41.0	29.0	41.0
25	35.872277597153506	40.0	36.0	41.0	28.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.577375533479433
1101	2	0.5519921134851451
1101	3	0.5443732587794621
1101	4	0.5843266579490134
1101	5	0.5540631436577996
1101	6	0.5614601483669119
1101	7	0.568378515532018
1101	8	0.5239807051796959
1101	9	0.6155441127415102
1101	10	0.5845526951867512
1101	11	0.4877509537013367
1101	12	0.5449771865809794
1101	13	0.43872589491209624
1101	14	0.5982638432618685
1101	15	0.6813180742100329
1101	16	0.6647101056667708
1101	17	0.6719196924967292
1101	18	0.735277834508473
1101	19	0.764058638518776
1101	20	0.6676033147665237
1101	21	0.6850106014009896
1101	22	0.7202209732662794
1101	23	0.7116048212669668
1101	24	0.7760198940677014
1101	25	0.8069778025045835
1102	1	0.4370492820150318
1102	2	0.4798198932226079
1102	3	0.4598145224078536
1102	4	0.4635534950177487
1102	5	0.4530948047082717
1102	6	0.4454623918132583
1102	7	0.4708484951783021
1102	8	0.4937683427258932
1102	9	0.635047074351597
1102	10	0.575720873960698
1102	11	0.503763866216417
1102	12	0.4288834148229981
1102	13	0.4687564205006396
1102	14	0.5923532523393007
1102	15	0.5593158351843499
1102	16	0.6335503157629816
1102	17	0.5883405417775194
1102	18	0.6825162532107854
1102	19	0.6860293000966422
1102	20	0.6383555770332165
1102	21	0.7038782174800744
1102	22	0.6992891646478085
1102	23	0.6977225413509629
1102	24	0.6507628028915278
1102	25	0.8187705100384335
1103	1	0.5947098926090746
1103	2	0.5388456225074343
1103	3	0.5752637666723537
1103	4	0.5784931813266496
1103	5	0.6325204902691581
1103	6	0.5833392259851067
1103	7	0.5943011656792621
1103	8	0.6289944515488415
1103	9	0.6400349824299525
1103	10	0.6911116745772361
1103	11	0.6911692216820526
1103	12	0.6347640797033307
1103	13	0.6242415859770603
1103	14	0.8060460128276006
1103	15	0.8017696799341678
1103	16	0.8120652224771447
1103	17	0.6928348147864085
1103	18	0.7468927890205137
1103	19	0.7825071935244097
1103	20	0.8035059343893707
1103	21	0.7702539202822223
1103	22	0.8917101524452917
1103	23	0.8627019196774555
1103	24	0.9037026617410291
1103	25	0.9680422939930224
1104	1	0.4861040588513177
1104	2	0.4921403786385099
1104	3	0.5233374073251191
1104	4	0.5417547713687512
1104	5	0.5637343920702804
1104	6	0.5312162269623641
1104	7	0.5228519282517468
1104	8	0.5229454367448128
1104	9	0.6357271747177933
1104	10	0.5999252077343158
1104	11	0.5545635183465123
1104	12	0.568233853196368
1104	13	0.5444660571104265
1104	14	0.5458159444072592
1104	15	0.5192977420964553
1104	16	0.5496611484733975
1104	17	0.5783181674868416
1104	18	0.6846609609065339
1104	19	0.6799938687711702
1104	20	0.6568133402682577
1104	21	0.6251366072053912
1104	22	0.6844032273977163
1104	23	0.6649681969693901
1104	24	0.7026463927041391
1104	25	0.7424358533090185
1105	1	0.38429184148451867
1105	2	0.4747464610653722
1105	3	0.4448435792720993
1105	4	0.500891472531876
1105	5	0.5296098743983677
1105	6	0.481936254978109
1105	7	0.4929975461014209
1105	8	0.5290134950820331
1105	9	0.4891905434263606
1105	10	0.5056806697495446
1105	11	0.495336323929493
1105	12	0.4624462350162517
1105	13	0.5143682270499212
1105	14	0.6189383104829247
1105	15	0.6166603283673808
1105	16	0.5180367389229374
1105	17	0.5652730416304337
1105	18	0.6329906296173249
1105	19	0.673716241399049
1105	20	0.5959916277918751
1105	21	0.5914264112469212
1105	22	0.5690116055991936
1105	23	0.5888597639222724
1105	24	0.590196085214302
1105	25	0.5791350964621813
1106	1	0.31374748382471296
1106	2	0.2885637914858883
1106	3	0.3503451325551623
1106	4	0.3877451940695167
1106	5	0.4079930384406225
1106	6	0.3295399610502514
1106	7	0.3642073247965456
1106	8	0.31662545832119093
1106	9	0.30513191884325863
1106	10	0.3875234643169563
1106	11	0.3689594285471003
1106	12	0.39320609222920666
1106	13	0.2950566059015358
1106	14	0.3672499577341739
1106	15	0.4665644569394942
1106	16	0.3748991932761072
1106	17	0.4887620063650786
1106	18	0.5036098046226414
1106	19	0.4107794282183619
1106	20	0.4575294578963849
1106	21	0.4836016235500509
1106	22	0.43013376026318184
1106	23	0.2594633244339235
1106	24	0.43198110045582183
1106	25	0.43931445421879545
1107	1	0.24785146641394817
1107	2	0.23752494056629914
1107	3	0.15279495623271444
1107	4	0.22333465209739245
1107	5	0.2590417323558256
1107	6	0.16590704146096868
1107	7	0.27901270822206925
1107	8	0.23713631381387046
1107	9	0.31522700353169597
1107	10	0.34629677427452776
1107	11	0.31817862459804047
1107	12	0.2526140076604264
1107	13	0.2930543257549587
1107	14	0.28412540949124576
1107	15	0.3003463720809094
1107	16	0.3093422454358006
1107	17	0.369460316491093
1107	18	0.38571282809468954
1107	19	0.39016332133716247
1107	20	0.36380086281378965
1107	21	0.3973864454385492
1107	22	0.40201763163283744
1107	23	0.276126827759164
1107	24	0.30223733657887664
1107	25	0.3510008414233923
1108	1	0.19863544393239252
1108	2	0.13167063083637842
1108	3	0.1316011176262606
1108	4	0.19798908769246282
1108	5	0.21282293134460417
1108	6	0.16978161375401868
1108	7	0.21560409630191657
1108	8	0.1377512848796627
1108	9	0.1396083295766033
1108	10	0.03531514990009299
1108	11	0.07510991192211947
1108	12	0.06366610587141963
1108	13	0.08388680521562009
1108	14	-0.01946921460349671
1108	15	0.15871920578826604
1108	16	0.10630049004603848
1108	17	0.03351798086701052
1108	18	-0.015016167700949268
1108	19	0.09429260452301236
1108	20	0.11139218885586644
1108	21	0.08425098560334021
1108	22	0.1872073599297721
1108	23	0.13143414803654707
1108	24	0.16071585347223305
1108	25	0.1261048870201762
1109	1	-0.0150398817678834
1109	2	-0.015438539006261465
1109	3	0.07839686562567394
1109	4	0.028303017662253183
1109	5	0.03792623462382494
1109	6	-0.041606583354237614
1109	7	-4.7180904777377464E-4
1109	8	0.04697986334622328
1109	9	0.06904279682624548
1109	10	-0.11899082049693988
1109	11	-0.048775630022966254
1109	12	0.010867959117078385
1109	13	-0.11677381056233571
1109	14	-7.192668215765252E-4
1109	15	-0.01065898333873605
1109	16	-0.052673062308066676
1109	17	-0.048106797607978535
1109	18	0.013258093797190895
1109	19	-0.06487330448325679
1109	20	-0.10485086870192362
1109	21	-0.004305674996345488
1109	22	-0.039913100199328255
1109	23	-0.03613672870819329
1109	24	-0.02919210076768053
1109	25	-0.10727002562227739
1110	1	0.0016849140483934377
1110	2	0.0013513374899147834
1110	3	0.029888043835399003
1110	4	-0.012675019187028624
1110	5	0.03309858840552238
1110	6	0.010655807348456392
1110	7	0.06973548688791453
1110	8	-0.0039016130067679455
1110	9	0.08620421244999221
1110	10	-0.08500893023536804
1110	11	0.06701480258553971
1110	12	0.0020243617036328487
1110	13	-0.007949077838354413
1110	14	-0.028232955268620685
1110	15	0.03300974399149226
1110	16	0.07605449326220537
1110	17	0.07310071758031
1110	18	0.05557807987938901
1110	19	0.04728695884521272
1110	20	0.12371109345112075
1110	21	0.027724809505876635
1110	22	0.04427878826588483
1110	23	0.08559582363476892
1110	24	0.09976979825369625
1110	25	0.10008997610533754
1111	1	-0.10266492297121488
1111	2	-0.1801811695477511
1111	3	-0.16591297179765263
1111	4	-0.11168742168855772
1111	5	-0.18704186138744205
1111	6	-0.14431363071842185
1111	7	-0.2136112263592409
1111	8	-0.1805173586722688
1111	9	-0.16566129414199082
1111	10	-0.16811069964887082
1111	11	-0.2958877698465372
1111	12	-0.34040764177450455
1111	13	-0.32651342049411625
1111	14	-0.314811794275208
1111	15	-0.3112781834127034
1111	16	-0.3353863943629207
1111	17	-0.392032803989359
1111	18	-0.30422413948959104
1111	19	-0.3364415507752696
1111	20	-0.3194411393703902
1111	21	-0.324865312964711
1111	22	-0.31225374011566487
1111	23	-0.24294334560786268
1111	24	-0.42726639828698865
1111	25	-0.341892877075594
1112	1	-0.2447856140083502
1112	2	-0.15710213327407985
1112	3	-0.21745152505867082
1112	4	-0.30575561324121026
1112	5	-0.19444834334096583
1112	6	-0.27592774716890744
1112	7	-0.2853240276832736
1112	8	-0.2929896586055776
1112	9	-0.26071247997472824
1112	10	-0.35267495816073335
1112	11	-0.3129213976076173
1112	12	-0.39191566947999945
1112	13	-0.36046127083120183
1112	14	-0.4176737003181543
1112	15	-0.3986236820416664
1112	16	-0.3923801737623833
1112	17	-0.35117292368082786
1112	18	-0.421405249745078
1112	19	-0.48552063341778506
1112	20	-0.4132730697577003
1112	21	-0.45334397242481117
1112	22	-0.35826060551718086
1112	23	-0.38733879988895126
1112	24	-0.43727619884361957
1112	25	-0.41841510839491036
1113	1	-0.41701964399441493
1113	2	-0.44022482100925586
1113	3	-0.3604617610506793
1113	4	-0.25184974048739406
1113	5	-0.32831970092860985
1113	6	-0.3459528129226257
1113	7	-0.3454008843274181
1113	8	-0.3420969585781606
1113	9	-0.3237612862167367
1113	10	-0.41791617749029797
1113	11	-0.418254227683704
1113	12	-0.5870198813995344
1113	13	-0.49507140066538113
1113	14	-0.4860281355607583
1113	15	-0.4697809698863509
1113	16	-0.450161298051448
1113	17	-0.4780926937404999
1113	18	-0.44540724826112665
1113	19	-0.5404037386900029
1113	20	-0.4270941186947752
1113	21	-0.5362660615655841
1113	22	-0.6242458687022605
1113	23	-0.5885188506265919
1113	24	-0.6406288994707623
1113	25	-0.5348655483875078
1114	1	-0.6191584294553962
1114	2	-0.6157321323182678
1114	3	-0.6444135409748846
1114	4	-0.6838680671073263
1114	5	-0.6751293002750955
1114	6	-0.6670624017959028
1114	7	-0.7404509960238883
1114	8	-0.7345764404102013
1114	9	-0.7745029540535882
1114	10	-0.8096332823001617
1114	11	-0.9091809419521084
1114	12	-0.9238450616728358
1114	13	-0.7833897985300098
1114	14	-1.0582089830801849
1114	15	-1.0554550147446662
1114	16	-0.9535152867524275
1114	17	-0.9450421935653495
1114	18	-1.021102765666086
1114	19	-0.8425845231860905
1114	20	-0.9895724668148702
1114	21	-1.0200879567651384
1114	22	-1.2012296539306249
1114	23	-1.0700770962636597
1114	24	-1.0244044406518
1114	25	-1.1076487644655728
1115	1	-0.6759519967113832
1115	2	-0.700818154096968
1115	3	-0.6964418247721547
1115	4	-0.5321595122312104
1115	5	-0.6584079455587144
1115	6	-0.6536870936805954
1115	7	-0.7242790637213901
1115	8	-0.714936744217141
1115	9	-0.7383027806874907
1115	10	-0.7597095950620201
1115	11	-0.8677720127511179
1115	12	-0.8460087706904105
1115	13	-0.8635146987413549
1115	14	-0.8797449860155524
1115	15	-0.8532578408403495
1115	16	-0.9190850242387327
1115	17	-0.9019165864064291
1115	18	-0.8651444206822987
1115	19	-0.9231495065512689
1115	20	-0.962329050287174
1115	21	-0.9527170317984712
1115	22	-1.1350394308792673
1115	23	-0.9827698444698569
1115	24	-0.9642844990201098
1115	25	-0.9932886293829597
1116	1	-0.28638131581406157
1116	2	-0.4690729710786208
1116	3	-0.4211428193063753
1116	4	-0.403662597846143
1116	5	-0.44263730126742473
1116	6	-0.40827312886320044
1116	7	-0.39378768187149404
1116	8	-0.3763683098501147
1116	9	-0.4720582623425642
1116	10	-0.40506070679325745
1116	11	-0.47218854381205233
1116	12	-0.6519262301963167
1116	13	-0.622867355861672
1116	14	-0.548179214814283
1116	15	-0.6142610866777929
1116	16	-0.6681329463425385
1116	17	-0.6240452228497873
1116	18	-0.5848474158635923
1116	19	-0.680632573011053
1116	20	-0.7024084956123531
1116	21	-0.6929431451660832
1116	22	-0.7058887224322987
1116	23	-0.6184379056944564
1116	24	-0.7011622585490755
1116	25	-0.7030777658165164
1201	1	0.4960099135364864
1201	2	0.5672697379460665
1201	3	0.542601371214861
1201	4	0.6109318299705322
1201	5	0.5393291103458537
1201	6	0.5864849942354482
1201	7	0.6073647118922878
1201	8	0.5843422626171701
1201	9	0.7112382582002965
1201	10	0.7053200763028613
1201	11	0.5336879744082879
1201	12	0.6632803766103663
1201	13	0.682392813004185
1201	14	0.7468237561465898
1201	15	0.8364634463388612
1201	16	0.6749231001349898
1201	17	0.6791773839162119
1201	18	0.7282370348646552
1201	19	0.7613010181728654
1201	20	0.6793493469190963
1201	21	0.7366910409728789
1201	22	0.7368049939642702
1201	23	0.7181508378815309
1201	24	0.6815946914226529
1201	25	0.6076198124053676
1202	1	0.4628514545616298
1202	2	0.4289011217757235
1202	3	0.47233592176029404
1202	4	0.5397078093418486
1202	5	0.5132935894599839
1202	6	0.5330936801890687
1202	7	0.5180625779423522
1202	8	0.44733095779309906
1202	9	0.5057360333589784
1202	10	0.6028802585517425
1202	11	0.5073631658878028
1202	12	0.4789137613822305
1202	13	0.4593349504832247
1202	14	0.5823865752444348
1202	15	0.5280546115630855
1202	16	0.5687219272033062
1202	17	0.5733161048114042
1202	18	0.6345573487562177
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2210	14	-0.04094839512023185
2210	15	0.053676248575321495
2210	16	0.15937286948443585
2210	17	0.051236328118591246
2210	18	0.06289956120967588
2210	19	0.11428398419695895
2210	20	0.10009056433590047
2210	21	-0.02163074033264678
2210	22	0.04883097471661557
2210	23	0.04066273701618428
2210	24	0.1132304616800397
2210	25	0.05619199424879184
2211	1	-0.16219193177252933
2211	2	-0.22477426663621358
2211	3	-0.20982969370612992
2211	4	-0.23288505329841058
2211	5	-0.28396852436193143
2211	6	-0.2667324964840603
2211	7	-0.219279732093014
2211	8	-0.21610347661986395
2211	9	-0.23664206990565617
2211	10	-0.32298014110291007
2211	11	-0.1468368298232008
2211	12	-0.23211617657829464
2211	13	-0.10208322070634068
2211	14	-0.1766091889785386
2211	15	-0.1553778451478749
2211	16	-0.23345723830176013
2211	17	-0.3555964291331861
2211	18	-0.24062145853856265
2211	19	-0.2569536289617176
2211	20	-0.24160889142834918
2211	21	-0.2516726763674697
2211	22	-0.20481863921455812
2211	23	-0.14389695465779795
2211	24	-0.2802055294267234
2211	25	-0.3086429202957248
2212	1	-0.2869368991212369
2212	2	-0.25968954915068565
2212	3	-0.3465999865658205
2212	4	-0.30275034083718566
2212	5	-0.2504738108431397
2212	6	-0.3091811412645029
2212	7	-0.4105439502286856
2212	8	-0.40024004200808605
2212	9	-0.38198099967088694
2212	10	-0.4126984210652367
2212	11	-0.27714363206327874
2212	12	-0.27121824076038337
2212	13	-0.3119544788434325
2212	14	-0.23950989406302625
2212	15	-0.3267626733856588
2212	16	-0.245263058982502
2212	17	-0.29156586194559253
2212	18	-0.48653003826993313
2212	19	-0.44607768546118365
2212	20	-0.36465140993649214
2212	21	-0.40358411706631614
2212	22	-0.344950363866559
2212	23	-0.3712459345397434
2212	24	-0.45865735060562685
2212	25	-0.46751998930758987
2213	1	-0.5019338058648835
2213	2	-0.5151723887182023
2213	3	-0.5776268979029702
2213	4	-0.5971540224532674
2213	5	-0.5805502953583499
2213	6	-0.5194602887457975
2213	7	-0.5686781819644935
2213	8	-0.5853713080597629
2213	9	-0.5115645828800197
2213	10	-0.47149840734820714
2213	11	-0.4383063536643306
2213	12	-0.5843856117274981
2213	13	-0.47028067908743765
2213	14	-0.7177662537217202
2213	15	-0.6778462139587376
2213	16	-0.558555342139158
2213	17	-0.6857709551187767
2213	18	-0.5541156637590845
2213	19	-0.6038947622683182
2213	20	-0.5855641840287618
2213	21	-0.5715174975426933
2213	22	-0.6369132599278444
2213	23	-0.6234163386792133
2213	24	-0.6070805508551018
2213	25	-0.5365009429135696
2214	1	-0.5350664524678379
2214	2	-0.6083845550011517
2214	3	-0.6181982844886207
2214	4	-0.7653333189072953
2214	5	-0.722258033513981
2214	6	-0.6104610866314246
2214	7	-0.6792917250217982
2214	8	-0.6361612034438195
2214	9	-0.6503809944644559
2214	10	-0.683282244318761
2214	11	-0.6066139384878468
2214	12	-0.5139375362722518
2214	13	-0.6262171911981298
2214	14	-0.8060123075617867
2214	15	-0.6509975358300508
2214	16	-0.6699216340773404
2214	17	-0.7530035475095218
2214	18	-0.7765551535700936
2214	19	-0.729628097978761
2214	20	-0.8682592681549224
2214	21	-0.8228732481776149
2214	22	-0.8127361430479425
2214	23	-0.7060143838889132
2214	24	-0.844521641594099
2214	25	-0.7932159178220886
2215	1	-0.6435918401718723
2215	2	-0.5738353881894156
2215	3	-0.7193184568372253
2215	4	-0.6887668757174623
2215	5	-0.6789375646468372
2215	6	-0.6569777394619223
2215	7	-0.6281479286383629
2215	8	-0.7163809609128009
2215	9	-0.8228595125054952
2215	10	-0.8055575256775
2215	11	-0.7140783448008605
2215	12	-0.7335776730790684
2215	13	-0.7110204502054884
2215	14	-0.8613502917758709
2215	15	-0.8340740373656388
2215	16	-0.8859121084821453
2215	17	-0.8473253062391137
2215	18	-0.8300900963481652
2215	19	-0.9207475395353768
2215	20	-0.8837306053141702
2215	21	-0.844439464131824
2215	22	-0.9421691313594209
2215	23	-0.847641996645585
2215	24	-0.8972956956374674
2215	25	-0.8786546520498177
2216	1	-0.01345647580428988
2216	2	-0.08887437484521499
2216	3	-0.05350186772797727
2216	4	0.07853367095524533
2216	5	0.009235203207737186
2216	6	0.08661864235481431
2216	7	0.05111459294123222
2216	8	0.10017941371295791
2216	9	0.16822029998949972
2216	10	0.15331959898815484
2216	11	0.2972187740463781
2216	12	0.18312026471329546
2216	13	0.13541069569049569
2216	14	0.235742713848353
2216	15	0.21943602944222107
2216	16	0.0669131248616992
2216	17	-0.0013716186319854273
2216	18	0.14123467699039338
2216	19	0.1487877900602399
2216	20	0.3009236093847889
2216	21	0.1978550242547783
2216	22	0.25361680357612215
2216	23	0.12883132854896928
2216	24	-0.030451825614910888
2216	25	-0.002583393759849173
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	194121.0
3	15.0
4	1610.0
5	9455.0
6	12283.0
7	12312.0
8	10576.0
9	10166.0
10	10217.0
11	12005.0
12	19634.0
13	15918.0
14	8092.0
15	8057.0
16	8582.0
17	9114.0
18	10208.0
19	12059.0
20	15188.0
21	19218.0
22	23690.0
23	28289.0
24	33546.0
25	38753.0
26	44330.0
27	50513.0
28	58977.0
29	71967.0
30	91968.0
31	118782.0
32	154999.0
33	207526.0
34	289250.0
35	412846.0
36	626202.0
37	1171890.0
38	3287859.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.50990046578497	17.271634100778112	12.362596813192509	25.85586862024441
2	17.385602353920277	20.1586259181971	36.79055513155014	25.66521659633248
3	18.829915139451998	23.848978261194212	27.903248211678843	29.41785838767495
4	13.119300261900138	15.840195541582036	36.401146013906946	34.63935818261088
5	16.152754290603095	34.68862746070522	32.37192863606121	16.78668961263046
6	33.76012400512109	36.04913536855115	15.667637227600597	14.523103398727159
7	30.095430438898962	30.169209752209582	20.68531033186197	19.05004947702949
8	27.617867136350817	31.355610682274772	19.460623142076642	21.56589903929777
9	27.729143055139403	14.602370035562778	18.330093338041447	39.33839357125637
10	17.106795507106927	25.02425926387225	29.487949742827173	28.380995486193644
11	37.38487933482282	21.69748587427417	21.533055090543137	19.384579700359872
12	25.845380950817187	23.395624809618013	27.022663524679736	23.736330714885067
13	29.712485252705118	19.558278266149838	24.34167438680624	26.387562094338808
14	24.633514352694185	19.92216063682428	23.88292049349975	31.561404516981785
15	25.5842286296658	26.047093294832052	22.210086769431115	26.158591306071028
16	26.12648397030375	25.796346318105932	22.568989596459733	25.508180115130585
17	25.645217200771874	24.88705735378816	23.52322456368264	25.944500881757328
18	27.499226020106914	23.175230038715274	24.41845337937246	24.907090561805354
19	27.83851263319595	24.108870330745525	23.62093107925311	24.431685956805417
20	26.927462623730523	23.77973174278009	23.442092139612438	25.85071349387695
21	27.411535170076057	24.06317826527715	23.413713828564827	25.111572736081968
22	26.382544316382848	23.782332784497928	24.22379801649224	25.611324882626977
23	25.0450665677469	23.08782521205736	24.657756622559905	27.209351597635834
24	25.555150898708572	24.914964265246102	23.89797495276343	25.6319098832819
25	25.447034581091245	23.96956545800071	24.200890169874487	26.382509791033566
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3444.0
1	3444.0
2	3391.5
3	3339.0
4	3339.0
5	3339.0
6	5669.0
7	7999.0
8	7999.0
9	7999.0
10	11208.0
11	14417.0
12	14417.0
13	14417.0
14	20221.5
15	26026.0
16	26026.0
17	26026.0
18	41812.0
19	57598.0
20	57598.0
21	57598.0
22	83742.0
23	109886.0
24	109886.0
25	109886.0
26	153767.0
27	197648.0
28	197648.0
29	197648.0
30	237229.5
31	276811.0
32	276811.0
33	276811.0
34	354863.5
35	432916.0
36	432916.0
37	432916.0
38	490329.0
39	547742.0
40	547742.0
41	547742.0
42	630250.5
43	712759.0
44	712759.0
45	712759.0
46	772291.5
47	831824.0
48	831824.0
49	831824.0
50	859288.5
51	886753.0
52	886753.0
53	886753.0
54	830514.5
55	774276.0
56	774276.0
57	774276.0
58	736524.0
59	698772.0
60	698772.0
61	698772.0
62	625985.5
63	553199.0
64	553199.0
65	553199.0
66	470342.0
67	387485.0
68	387485.0
69	387485.0
70	306936.0
71	226387.0
72	226387.0
73	226387.0
74	189619.5
75	152852.0
76	152852.0
77	152852.0
78	131249.5
79	109647.0
80	109647.0
81	109647.0
82	83442.5
83	57238.0
84	57238.0
85	57238.0
86	42859.0
87	28480.0
88	28480.0
89	28480.0
90	19995.0
91	11510.0
92	11510.0
93	11510.0
94	6245.0
95	980.0
96	980.0
97	980.0
98	604.5
99	229.0
100	229.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1398691488600137
2	0.15823708334077566
3	0.17185129511518427
4	0.1915412708219735
5	0.18231511077650653
6	0.0661583183748119
7	0.054695939659788154
8	0.05857767772769804
9	0.06686153179291152
10	0.06587703300757206
11	0.6790088122486276
12	0.6395444752248771
13	0.637983341436696
14	0.6386724905864336
15	0.6401633030328048
16	0.6454233394001899
17	0.6377864416796281
18	0.6480814861206065
19	0.6443263264679545
20	0.6494316558833577
21	0.6373785778971303
22	0.6407821308407324
23	0.6266756696736541
24	0.6363518863067049
25	0.6304167650579441
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	7110217.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.14671726216652
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.17702783468789	34.634919575937786
2	11.03948486859861	9.305560949535822
3	2.757587585165856	3.4866979283293755
4	1.1374827673698524	1.9176465834769558
5	0.6147356454572026	1.295454472003008
6	0.4009268480204403	1.0138650303797463
7	0.26432655542056094	0.779834761733494
8	0.20453479648578393	0.6896376190208884
9	0.15527178629645105	0.5889776468245258
>10	0.989655974839311	8.032875704680787
>50	0.11603830397099547	3.4117805855370396
>100	0.10745234689955019	9.241066739803191
>500	0.01629557432357171	4.741707750892363
>1k	0.01736849695164363	15.13363034552678
>5k	0.0016429659278440905	4.832383346538326
>10k+	1.676495844738868E-4	0.8939609597799338
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAAGGGCAGGGACTTAATCAA	17264	0.24280552900143557	No Hit
GTATCTGATCGTCTTCGAACCTCCG	12168	0.17113401742872264	No Hit
GAATAGGACCGCGGTTCTATTTTGT	11880	0.16708350814046885	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	11033	0.15517107283786136	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	10914	0.15349742490278426	No Hit
CTATTGGAGCTGGAATTACCGCGGC	9966	0.14016449849561552	No Hit
GAACTACGACGGTATCTGATCGTCT	9961	0.1400941771538056	No Hit
GTCCTATTCCATTATTCCTAGCTGC	9943	0.1398410203232897	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	9302	0.13082582430325262	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	9152	0.12871618404895377	No Hit
GTATCAACGCAGAGTACGGGCGGTG	8859	0.12459535341889003	No Hit
TCGTAGTTCCGACCATAAACGATGC	8728	0.12275293426346903	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	8720	0.1226404201165731	No Hit
ATCAGATACCGTCGTAGTTCCGACC	8643	0.12155747145269968	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	8601	0.120966772181496	No Hit
CTTATGACCCGCACTTACTGGGAAT	8546	0.12019323742158641	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	8494	0.11946189546676284	No Hit
GAATAACGCCGCCGCATCGCCAGTC	8235	0.11581924996100681	No Hit
GACTTAATCAACGCAAGCTTATGAC	8230	0.11574892861919686	No Hit
CTTTAATATACGCTATTGGAGCTGG	8169	0.11489100824911531	No Hit
GAATAATTGCAATCCCCGATCCCCA	7928	0.11150151957387516	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	7792	0.10958877907664422	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	7544	0.10610084052287011	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	7511	0.10563671966692437	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	7391	0.10394900746348529	No Hit
GCTTTGAACACTCTAATTTTTTCAA	7355	0.10344269380245356	No Hit
CTCTAGATAACCTCGGGCCGATCGC	7283	0.10243006648039013	No Hit
GAATAATGGAATAGGACCGCGGTTC	7113	0.10003914085885142	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.8128536723984656E-5
2	0.0	0.0	0.0	0.0	2.8128536723984656E-5
3	0.0	0.0	0.0	0.0	2.8128536723984656E-5
4	0.0	0.0	0.0	1.4064268361992328E-5	2.8128536723984656E-5
5	0.0	0.0	0.0	1.4064268361992328E-5	4.219280508597698E-5
6	0.0	0.0	0.0	1.4064268361992328E-5	7.032134180996163E-5
7	0.0	0.0	0.0	2.8128536723984656E-5	7.032134180996163E-5
8	0.0	0.0	0.0	2.8128536723984656E-5	7.032134180996163E-5
9	0.0	0.0	0.0	7.032134180996163E-5	7.032134180996163E-5
10	0.0	0.0	0.0	1.1251414689593862E-4	7.032134180996163E-5
11	0.0	0.0	0.0	1.1251414689593862E-4	7.032134180996163E-5
12	0.0	1.4064268361992328E-5	0.0	1.2657841525793095E-4	1.968997570678926E-4
13	0.0	1.4064268361992328E-5	0.0	1.4064268361992327E-4	2.531568305158619E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4020	0.0	15.494889	1
GTATCAA	13975	0.0	13.581608	1
AACCGCG	1010	0.0	11.769704	7
CCGTCCA	385	0.0	11.610088	9
CGGTTCT	2220	0.0	11.478982	12
AGGCCCG	2140	0.0	11.465799	10
CGTCGTA	1440	0.0	11.293591	10
CTCGACG	245	0.0	11.254887	13
CGACCAT	1665	0.0	11.195416	10
GGTTCTA	2250	0.0	11.156569	13
ACCGTCC	435	0.0	11.150116	8
ACTCTAA	1445	0.0	10.991249	10
AGAACCG	1155	0.0	10.868404	5
CGAGCCG	2250	0.0	10.86052	15
CCGACCA	1780	0.0	10.846122	9
TGCGTTA	195	1.8189894E-12	10.72747	13
CCGTCGT	1605	0.0	10.725115	9
GGTAGCG	375	0.0	10.651639	11
ACGCTTA	260	0.0	10.6048155	17
GCGCGCT	930	0.0	10.530628	15
>>END_MODULE