FastQCFastQC Report
Thu 2 Feb 2017
SRR4050285_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050285_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7110217
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA167020.23490141018199587No Hit
GAATAGGACCGCGGTTCTATTTTGT123140.17318740060957352No Hit
GATTAAGAGGGACGGCCGGGGGCAT116580.16396124056410655No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG107590.15131746330667545No Hit
GTATCTGATCGTCTTCGAACCTCCG103300.14528389217938073No Hit
CTATTGGAGCTGGAATTACCGCGGC98860.13903935702665615No Hit
ATCAGATACCGTCGTAGTTCCGACC97910.13770325153226687No Hit
GAACTACGACGGTATCTGATCGTCT97380.13695784530908128No Hit
GTATCAACGCAGAGTACTTTTTTTT97080.13653591725822153No Hit
TCGTAGTTCCGACCATAAACGATGC93970.13216192979764188No Hit
CTTATGACCCGCACTTACTGGGAAT91120.12815361331447409No Hit
ATACAGGACTCTTTCGAGGCCCTGT91020.12801297063085418No Hit
GTCCTATTCCATTATTCCTAGCTGC87230.12268261292165906No Hit
GAATAACGCCGCCGCATCGCCAGTC84950.11947595973512481No Hit
CTCTTAATCATGGCCTCAGTTCCGA83510.11745070509099793No Hit
CTTTAATATACGCTATTGGAGCTGG82400.11588957130281678No Hit
GGGTAGGCACACGCTGAGCCAGTCA82140.11552390032540498No Hit
GTATCAACGCAGAGTACGGGCGGTG81950.11525667922652712No Hit
GCGCAAGACGGACCAGAGCGAAAGC81310.1143565660513596No Hit
GACTTAATCAACGCAAGCTTATGAC80260.11287981787335043No Hit
ATATTAAAGTTGCTGCAGTTAAAAA79400.11167029079421908No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC78490.11039044237327778No Hit
GAATAATTGCAATCCCCGATCCCCA76730.10791513114156712No Hit
GTCTTGCGCCGGTCCAAGAATTTCA76700.10787293833648115No Hit
GCTTTGAACACTCTAATTTTTTCAA72290.10167059598884254No Hit
GTGCATGGCCGTTCTTAGTTGGTGG71730.10088299696057097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42200.015.6274821
GTATCAA134500.013.3511251
CTCGACG3400.012.84321813
AACCGCG10250.011.9521797
CGTCGTA17200.011.53892810
TCGACGC3750.011.39145614
ACGCTTA3750.011.39129517
TAGATCG2050.011.1231585
CGCTTAC3850.011.09541718
ATACCGT19050.010.9703126
TACCGTC19150.010.9598537
AGAACCG12450.010.9128315
ACGCATC6100.010.897222510
CCGTCGT18850.010.7807789
GAACCGC12700.010.7708526
GACGCTT3900.010.7098416
ACCGTCC4000.010.683178
CGACCAT16650.010.66534810
GTCGTAG18700.010.61334711
TGCGTAG3400.010.60961413
Produced by FastQC (version 0.11.5)