FastQCFastQC Report
Thu 2 Feb 2017
SRR4050284_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050284_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794887
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA339884.2758278849698135No Hit
CTTCCGTACTCTGCGTTGATACCAC216942.7291929544702582No Hit
GAGTACGGAAGCAGTGGTATCAACG169962.138165550575113No Hit
TCCGTACTCTGCGTTGATACCACTG166402.0933793105183502No Hit
GCTTCCGTACTCTGCGTTGATACCA134191.68816448124073No Hit
GTACATGGGAAGCAGTGGTATCAAC116221.4620946121901603No Hit
GTACATGGAAGCAGTGGTATCAACG100901.2693628150919565No Hit
CCCATGTACTCTGCGTTGATACCAC98301.2366537633651074No Hit
GCGTTGATACCACTGCTTCCGTACT84911.0682021469718337No Hit
TCCATGTACTCTGCGTTGATACCAC81451.0246739473661035No Hit
GTATCAACGCAGAGTACGGAAGCAG70290.8842766330308585No Hit
TATCAACGCAGAGTACGGAAGCAGT53990.6792160395125345No Hit
ACTCTGCGTTGATACCACTGCTTCC53480.6728000332122679No Hit
CATGTACTCTGCGTTGATACCACTG39670.4990646469246572No Hit
GAGTACATGGGAAGCAGTGGTATCA37970.47767795925710194No Hit
ATACCACTGCTTCCGTACTCTGCGT36300.45666868372485653No Hit
CAGTGGTATCAACGCAGAGTACGGA36300.45666868372485653No Hit
GGTATCAACGCAGAGTACGGAAGCA35400.44534631966556254No Hit
ACGCAGAGTACGGAAGCAGTGGTAT34650.4359110162828176No Hit
GAGTACATGGAAGCAGTGGTATCAA33100.4164113892918113No Hit
GTATCAACGCAGAGTACATGGGAAG27700.3484772049360475No Hit
AAGCAGTGGTATCAACGCAGAGTAC25480.3205487069231224No Hit
GAAGCAGTGGTATCAACGCAGAGTA25410.31966807860739954No Hit
GTACTCTGCGTTGATACCACTGCTT24680.3104843833148611No Hit
GCGTTGATACCACTGCTTCCCATGT24450.307590890277486No Hit
GTGGTATCAACGCAGAGTACGGAAG24320.30595543769114353No Hit
TATCAACGCAGAGTACATGGGAAGC21200.2667045756189244No Hit
GCTTCCATGTACTCTGCGTTGATAC20240.2546273872890109No Hit
CATGGAAGCAGTGGTATCAACGCAG20190.25399836706349455No Hit
GATACCACTGCTTCCGTACTCTGCG19760.24858879312405413No Hit
GCTTCCCATGTACTCTGCGTTGATA19080.24003411805703204No Hit
CAGTGGTATCAACGCAGAGTACATG19010.23915348974130915No Hit
GTTGATACCACTGCTTCCGTACTCT18920.23802125333537974No Hit
ACGCAGAGTACATGGGAAGCAGTGG17880.22493763264464006No Hit
ATCAACGCAGAGTACGGAAGCAGTG17730.22305057196809105No Hit
CCACTGCTTCCGTACTCTGCGTTGA17640.22191833556216167No Hit
GCGTTGATACCACTGCTTCCATGTA16730.21047016745776442No Hit
CTGCTTCCGTACTCTGCGTTGATAC16240.2043057692477044No Hit
CGGAAGCAGTGGTATCAACGCAGAG15760.19826717508274763No Hit
GTATCAACGCAGAGTACATGGAAGC14690.1848061422566981No Hit
GGTATCAACGCAGAGTACATGGGAA14270.17952237236236096No Hit
AAAAAGTACTCTGCGTTGATACCAC13350.16794840021286042No Hit
GTACGGGAAGCAGTGGTATCAACGC12710.1598969413262514No Hit
TATCAACGCAGAGTACATGGAAGCA12310.15486477952212074No Hit
GTATCAACGCAGAGTACTTTTTTTT12060.1517196783945391No Hit
CCATGTACTCTGCGTTGATACCACT11980.15071324603371297No Hit
CCCGTACTCTGCGTTGATACCACTG11970.15058744198860968No Hit
CACTGCTTCCGTACTCTGCGTTGAT11170.1405231183803484No Hit
GCAGAGTACGGAAGCAGTGGTATCA10800.13586836871152755No Hit
GCAGTGGTATCAACGCAGAGTACGG10540.13259746353884264No Hit
TGATACCACTGCTTCCGTACTCTGC10210.12844593005043484No Hit
ACGCAGAGTACATGGAAGCAGTGGT10030.12618145723857604No Hit
TTCCCGTACTCTGCGTTGATACCAC9100.11448168104397229No Hit
ATACCACTGCTTCCCATGTACTCTG9050.11385266081845595No Hit
GGGAAGCAGTGGTATCAACGCAGAG9000.11322364059293963No Hit
TACCACTGCTTCCGTACTCTGCGTT8780.11045595160066776No Hit
GTACGGGAGCAGTGGTATCAACGCA8660.10894630305942857No Hit
AAAGTACTCTGCGTTGATACCACTG8470.10655602620246651No Hit
CTGCGTTGATACCACTGCTTCCGTA8350.10504637766122732No Hit
CATGGGAAGCAGTGGTATCAACGCA8100.10190127653364567No Hit
CTGCTTCCCATGTACTCTGCGTTGA8010.10076904012771626No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT307.855612E-418.9501951
TTAGGCA453.6531528E-516.8244654
GATTTAT350.002207298516.2430251
TTATACT350.002225019516.223594
CCCAACT601.5241258E-615.7729364
GCACGCC456.733139E-414.78471315
AGGCCCG1058.54925E-1114.4994910
TTTCGTA400.0054115614.1956423
TTCAAGA400.0054115614.1956422
CGAGGTT400.0054115614.1956424
CACTCTA1101.8007995E-1013.8404219
AACGAAC551.9504587E-413.82466614
ACGAACG551.9504587E-413.82466615
AACCGCG1051.3333192E-913.5932717
TAGAGTG852.641118E-713.433355
CGCGGTC1009.818905E-913.32140510
GGTCCTA1009.977157E-913.30624313
ACGCCCC500.001495971713.30287717
CACGCCC500.001495971713.30287716
GTATTTT500.001528346413.2651371
Produced by FastQC (version 0.11.5)