FastQCFastQC Report
Thu 2 Feb 2017
SRR4050284_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050284_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences794887
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA285913.5968634535474853No Hit
CTTCCGTACTCTGCGTTGATACCAC207712.6130758208399434No Hit
TCCGTACTCTGCGTTGATACCACTG171152.153136231942402No Hit
GAGTACGGAAGCAGTGGTATCAACG160922.0244386938017604No Hit
GCTTCCGTACTCTGCGTTGATACCA130651.6436298492741734No Hit
CCCATGTACTCTGCGTTGATACCAC101591.2780432942040818No Hit
GTACATGGGAAGCAGTGGTATCAAC98941.2447052222517163No Hit
TCCATGTACTCTGCGTTGATACCAC89511.1260720077193362No Hit
GTACATGGAAGCAGTGGTATCAACG86371.0865695375569104No Hit
GCGTTGATACCACTGCTTCCGTACT80451.012093542855777No Hit
GTATCAACGCAGAGTACGGAAGCAG68250.8586126078297921No Hit
TATCAACGCAGAGTACGGAAGCAGT60080.7558307029804237No Hit
ACTCTGCGTTGATACCACTGCTTCC58510.7360794678992109No Hit
CATGTACTCTGCGTTGATACCACTG43950.5529087782288552No Hit
CAGTGGTATCAACGCAGAGTACGGA42040.5288802056141313No Hit
ACGCAGAGTACGGAAGCAGTGGTAT39650.4988130388344507No Hit
GGTATCAACGCAGAGTACGGAAGCA37010.4656007709271884No Hit
ATACCACTGCTTCCGTACTCTGCGT36420.45817833226609567No Hit
GAGTACATGGGAAGCAGTGGTATCA34990.4401883538163286No Hit
GAGTACATGGAAGCAGTGGTATCAA31440.3955279178046691No Hit
AAGCAGTGGTATCAACGCAGAGTAC30980.38974093172991886No Hit
GAAGCAGTGGTATCAACGCAGAGTA28880.3633220822582329No Hit
GTGGTATCAACGCAGAGTACGGAAG27140.34143217841026463No Hit
GTATCAACGCAGAGTACATGGGAAG25480.3205487069231224No Hit
GCGTTGATACCACTGCTTCCCATGT23690.29802978284963777No Hit
CATGGAAGCAGTGGTATCAACGCAG23210.291991188684681No Hit
TATCAACGCAGAGTACATGGGAAGC22870.28771385115116993No Hit
ACGCAGAGTACATGGGAAGCAGTGG20680.26016276527355464No Hit
ATCAACGCAGAGTACGGAAGCAGTG20630.2595337450480383No Hit
GCTTCCCATGTACTCTGCGTTGATA20000.2516080902065325No Hit
GCTTCCATGTACTCTGCGTTGATAC19710.24795977289853777No Hit
GTTGATACCACTGCTTCCGTACTCT19570.24619851626709205No Hit
GTACTCTGCGTTGATACCACTGCTT19310.24292761109440714No Hit
GATACCACTGCTTCCGTACTCTGCG19200.2415437665982712No Hit
GTATCAACGCAGAGTACTTTTTTTT19160.24104055041785813No Hit
CCACTGCTTCCGTACTCTGCGTTGA18820.23676321288434707No Hit
CAGTGGTATCAACGCAGAGTACATG18780.236259996703934No Hit
CGGAAGCAGTGGTATCAACGCAGAG17380.21864743038947673No Hit
GCGTTGATACCACTGCTTCCATGTA17000.2138668766755526No Hit
CTGCTTCCGTACTCTGCGTTGATAC15510.19512207395516595No Hit
TATCAACGCAGAGTACTTTTTTTTT14940.18795124338427976No Hit
AAAAAGTACTCTGCGTTGATACCAC14510.18254166944483935No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13990.1759998590994695No Hit
GTATCAACGCAGAGTACATGGAAGC13570.17071608920513232No Hit
CCATGTACTCTGCGTTGATACCACT13240.16656455571672452No Hit
GGTATCAACGCAGAGTACATGGGAA13180.16580973144610492No Hit
CCCGTACTCTGCGTTGATACCACTG12870.16190980604790367No Hit
GCAGAGTACGGAAGCAGTGGTATCA12680.15951952919094162No Hit
TATCAACGCAGAGTACATGGAAGCA12650.1591421170556318No Hit
ACGCAGAGTACATGGAAGCAGTGGT12270.1543615633417077No Hit
GCAGTGGTATCAACGCAGAGTACGG12220.15373254311619136No Hit
CACTGCTTCCGTACTCTGCGTTGAT11420.14366821950793005No Hit
GTACGGGAAGCAGTGGTATCAACGC11380.143165003327517No Hit
TGATACCACTGCTTCCGTACTCTGC10850.13649738893704388No Hit
CATGGGAAGCAGTGGTATCAACGCA9840.12379118038161399No Hit
GGGAAGCAGTGGTATCAACGCAGAG9770.12291055206589113No Hit
TACCACTGCTTCCGTACTCTGCGTT9560.12026866711872253No Hit
AAAGTACTCTGCGTTGATACCACTG9490.11938803880299967No Hit
ATACCACTGCTTCCCATGTACTCTG9280.11674615385583109No Hit
TTCCCGTACTCTGCGTTGATACCAC9180.11548811340479842No Hit
GGTATCAACGCAGAGTACTTTTTTT9070.11410426890866249No Hit
CTGCGTTGATACCACTGCTTCCGTA8970.11284622845762983No Hit
CTGCTTCCCATGTACTCTGCGTTGA8780.11045595160066776No Hit
ATACCACTGCTTCCATGTACTCTGC8320.10466896552591752No Hit
GCAGTGGTATCAACGCAGAGTACAT8210.10328512102978159No Hit
TACCACTGCTTCCCATGTACTCTGC8150.10253029675916199No Hit
CCCCATGTACTCTGCGTTGATACCA8030.1010206482179228No Hit
GTGGTATCAACGCAGAGTACATGGG8030.1010206482179228No Hit
TACGGAAGCAGTGGTATCAACGCAG8030.1010206482179228No Hit
TCAACGCAGAGTACGGAAGCAGTGG8020.10089484417281955No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAA402.6911582E-416.6793251
TTTAAGG508.585791E-515.2246682
CGACCAT902.3028406E-914.76823610
GACCATA902.3028406E-914.76823611
TTTTCGG1101.2732926E-1114.67049216
TTCGGAA1058.913048E-1114.46502418
CTCTTGA400.00516310814.2965641
TCCTTAT400.00516310814.2965641
CCCTATA400.005219632314.2731262
CGAGGTT400.005221816714.2722254
CTCGTAG400.00529875614.24079910
GCTCGTA400.00529875614.2407999
CGGTCCA602.5808165E-514.24079810
CCGACCA954.9312803E-913.990969
AGCCCGT551.9047911E-413.8633361
AAAGCTC551.9637648E-413.8136056
GTTTTCG1205.2750693E-1113.44795115
CGCCAGT1205.2750693E-1113.44795118
GCACGCC852.7188253E-713.40141915
ATACAGT500.001502167213.2955956
Produced by FastQC (version 0.11.5)