##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050284_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	794887
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.52407700717209	31.0	31.0	34.0	27.0	34.0
2	30.380428916311374	31.0	30.0	34.0	26.0	34.0
3	30.715865273932017	31.0	31.0	34.0	27.0	34.0
4	34.299914327445286	37.0	35.0	37.0	30.0	37.0
5	34.21110547788554	37.0	35.0	37.0	30.0	37.0
6	34.22978612054292	37.0	35.0	37.0	30.0	37.0
7	34.14051305405674	37.0	35.0	37.0	30.0	37.0
8	34.13619042706699	37.0	35.0	37.0	30.0	37.0
9	35.694777999891805	39.0	37.0	39.0	30.0	39.0
10	35.621639302190125	39.0	35.0	39.0	30.0	39.0
11	35.61522958609211	39.0	35.0	39.0	30.0	39.0
12	35.54115742237576	39.0	35.0	39.0	30.0	39.0
13	35.45699074208032	39.0	35.0	39.0	30.0	39.0
14	36.617246224935116	40.0	37.0	41.0	30.0	41.0
15	36.56349896274565	40.0	37.0	41.0	30.0	41.0
16	36.448398325799765	39.0	36.0	41.0	30.0	41.0
17	36.451782454613046	40.0	37.0	41.0	30.0	41.0
18	36.35858933408145	40.0	36.0	41.0	30.0	41.0
19	36.45597927755769	40.0	37.0	41.0	30.0	41.0
20	36.42669209585765	40.0	37.0	41.0	30.0	41.0
21	36.37267812909256	40.0	37.0	41.0	30.0	41.0
22	36.33881419623167	40.0	37.0	41.0	30.0	41.0
23	36.24737855821016	40.0	37.0	41.0	30.0	41.0
24	36.15474274959837	40.0	36.0	41.0	30.0	41.0
25	35.92008801250996	40.0	36.0	41.0	29.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	1.0220378715604497
1101	2	1.282775968529009
1101	3	1.0557211600321956
1101	4	0.9774189927860562
1101	5	1.1184600716029252
1101	6	1.0400229455622707
1101	7	1.0251289257141991
1101	8	1.0549537588797406
1101	9	1.2437958249010492
1101	10	1.2619928956598656
1101	11	1.3019405152065886
1101	12	1.3310090124707017
1101	13	1.31072980730913
1101	14	1.4111736923254625
1101	15	1.3775970992103908
1101	16	1.3392170163365833
1101	17	1.5800973274121333
1101	18	1.4648294920828775
1101	19	1.4091332121624234
1101	20	1.4836259197770048
1101	21	1.1793619650055476
1101	22	1.3834554719015841
1101	23	1.5234628542089652
1101	24	1.7074577984703296
1101	25	1.601445250117834
1102	1	0.517083619561646
1102	2	0.9097941448084583
1102	3	0.7350215255886532
1102	4	0.736605335725045
1102	5	0.5697472685758598
1102	6	0.5537600388539872
1102	7	0.5811167832540676
1102	8	0.689398636813813
1102	9	0.7131410566339156
1102	10	0.7140977847102761
1102	11	0.7594111744908645
1102	12	0.6981881618571606
1102	13	1.110578293777131
1102	14	1.0160513858993667
1102	15	1.0836060671012788
1102	16	1.1483727626275737
1102	17	1.2151220719501694
1102	18	1.2376907997488757
1102	19	1.0458013574443967
1102	20	0.9493002217993265
1102	21	0.9981129002742648
1102	22	1.0702366510883792
1102	23	1.1550852247677739
1102	24	1.0998698297670941
1102	25	0.8836552366499646
1103	1	0.638092669047996
1103	2	1.055293972644236
1103	3	0.7698929707317852
1103	4	0.6442744996086702
1103	5	0.810063114704505
1103	6	0.7653227885339007
1103	7	0.7248040394243986
1103	8	0.6805778112947252
1103	9	0.9054274309222734
1103	10	0.7540345352526785
1103	11	0.6433865299347659
1103	12	0.7719968021609773
1103	13	0.8561556790873439
1103	14	0.9650499918706217
1103	15	1.0112268912831652
1103	16	1.0344669080411535
1103	17	1.0434325559154942
1103	18	1.0778120835925122
1103	19	1.0921105242698559
1103	20	1.110663227552621
1103	21	1.132015879885337
1103	22	1.2333634207861408
1103	23	1.1051074556817824
1103	24	1.2077014631876821
1103	25	1.3038649761303986
1104	1	0.42939335042153814
1104	2	0.6184829357421222
1104	3	0.6000859141305561
1104	4	0.5168306623287648
1104	5	0.47486106383760784
1104	6	0.3474350465113645
1104	7	0.35511166945880035
1104	8	0.30123827139052395
1104	9	0.5294272959968254
1104	10	0.2814007903967095
1104	11	0.4269998335845031
1104	12	0.4603527355078043
1104	13	0.5363283836680637
1104	14	0.6203222039734371
1104	15	0.6682194029204851
1104	16	0.6843690870872337
1104	17	0.7564832204233554
1104	18	0.7983578570526788
1104	19	0.8531744114721747
1104	20	0.7824773002774421
1104	21	0.6622324352385078
1104	22	0.6411992165090012
1104	23	0.7326491139156062
1104	24	0.6753395931208956
1104	25	0.6672158497727096
1105	1	0.5179079037838648
1105	2	0.870135662406561
1105	3	0.7728895273791352
1105	4	0.5504271829042082
1105	5	0.5538239276716652
1105	6	0.5101014901379983
1105	7	0.6512943392480324
1105	8	0.712750482832476
1105	9	0.7662924682952479
1105	10	0.7367069128456905
1105	11	0.6817673143472973
1105	12	0.8092118857252615
1105	13	0.8966629437215872
1105	14	0.7918779082223537
1105	15	0.712501771605659
1105	16	0.8767210442635474
1105	17	0.7861620427719629
1105	18	0.9154706037907161
1105	19	0.7843520628605987
1105	20	0.7492153397555228
1105	21	0.8322023248336876
1105	22	1.0016241077769834
1105	23	0.8807822688169153
1105	24	1.0844663913811772
1105	25	0.9884169203648838
1106	1	0.4898178985510029
1106	2	0.7463176351956768
1106	3	0.572548083109119
1106	4	0.6558307674149333
1106	5	0.5451216775408412
1106	6	0.44494450426672216
1106	7	0.4567247421784302
1106	8	0.5883967413358775
1106	9	0.6466509193679855
1106	10	0.6765930980890147
1106	11	0.6741992871364104
1106	12	0.7495945387864893
1106	13	0.6783917506289683
1106	14	0.6598030782903805
1106	15	0.6287779359175403
1106	16	0.7796691081044642
1106	17	0.7808395676280568
1106	18	0.7574872181287589
1106	19	0.7455451554822616
1106	20	0.7590982544598717
1106	21	0.7513297450325354
1106	22	0.7292719361390994
1106	23	0.884566936530149
1106	24	0.8625269617631801
1106	25	0.9162858371623699
1107	1	0.5029000544903006
1107	2	0.7840760077754751
1107	3	0.717661897865792
1107	4	0.7228604639089156
1107	5	0.6975287942336763
1107	6	0.5660198574849318
1107	7	0.7361922984317033
1107	8	0.6674129632092445
1107	9	0.6557430177457988
1107	10	0.6896425486384672
1107	11	0.5627720239234364
1107	12	0.7302165953013997
1107	13	0.5366629437215877
1107	14	0.646917154690172
1107	15	0.9028000447610793
1107	16	0.9025923158491054
1107	17	0.8279516817044623
1107	18	0.5206511375426786
1107	19	0.6097523768323398
1107	20	0.7062954025498769
1107	21	0.8327988711445187
1107	22	0.6605252066780878
1107	23	0.44458132690168384
1107	24	0.5880142720719164
1107	25	0.8380872500352083
1108	1	0.29488215100418813
1108	2	0.5416784431977533
1108	3	0.3821777071872994
1108	4	0.4510179538969368
1108	5	0.2938726018568474
1108	6	0.43906135779511146
1108	7	0.24566467662694436
1108	8	0.4860324908812146
1108	9	0.2811139167499235
1108	10	0.4267242510358358
1108	11	0.2875891286536927
1108	12	0.23154601969061872
1108	13	0.34939458353262154
1108	14	0.35949780419321087
1108	15	0.3771035499631452
1108	16	0.19516601539307032
1108	17	0.49996928663599505
1108	18	0.22800341114592726
1108	19	0.3227979883859149
1108	20	0.3651753823852033
1108	21	0.4577782231587264
1108	22	0.429631170305818
1108	23	0.2695433040182138
1108	24	0.04936307803713902
1108	25	0.13263948546262583
1109	1	0.011138421442616675
1109	2	0.4424504579241386
1109	3	0.28669621894814057
1109	4	0.33478103127780656
1109	5	0.2300321454442411
1109	6	0.2060554475261398
1109	7	0.05340858007397742
1109	8	0.10476228205334337
1109	9	0.1791478476818824
1109	10	0.06297973776512578
1109	11	0.21800983963706955
1109	12	0.09722317193652685
1109	13	0.24359017651573112
1109	14	0.19302512594683208
1109	15	0.11127247234471582
1109	16	0.22803771547248175
1109	17	-0.05641583048259946
1109	18	0.17016384007973073
1109	19	0.17300300792047807
1109	20	0.04020977680764304
1109	21	0.0866307199152061
1109	22	0.31246175626922224
1109	23	0.44585336874156667
1109	24	0.2908238628850981
1109	25	0.270669032521603
1110	1	0.07135016286272489
1110	2	0.40905051535680315
1110	3	0.0809288639596879
1110	4	0.22003258397702297
1110	5	0.10248475756253583
1110	6	0.059021892135653786
1110	7	-0.045391029763621304
1110	8	0.06145817134594722
1110	9	0.15369058161913074
1110	10	0.12777541275320203
1110	11	0.3007728017386313
1110	12	0.2393037532879987
1110	13	0.1598363822813056
1110	14	0.11159059087849243
1110	15	0.24776226855368577
1110	16	0.13476777957398411
1110	17	0.16253789825010756
1110	18	0.29823156315936217
1110	19	0.3364119925726641
1110	20	0.417284887816443
1110	21	0.23506686642071628
1110	22	0.11840935964208654
1110	23	0.27240197410083766
1110	24	0.282251088341539
1110	25	0.2551337339785462
1111	1	0.27779757263546045
1111	2	0.456240755924604
1111	3	0.29293167635717765
1111	4	0.4624808608674229
1111	5	0.33784698564200966
1111	6	0.4309339324543302
1111	7	0.4673221079865044
1111	8	0.2383587762637447
1111	9	0.40619329422926853
1111	10	0.5635192414217656
1111	11	0.38352920361325715
1111	12	0.4063191021881565
1111	13	0.5013631746112139
1111	14	0.38616982088719
1111	15	0.4126161317004957
1111	16	0.41469882307438866
1111	17	0.4531553640184569
1111	18	0.3439800485709057
1111	19	0.48008168956321384
1111	20	0.6089993262430582
1111	21	0.5575509061796637
1111	22	0.6534221026013398
1111	23	0.6202606752136433
1111	24	0.7230984091085446
1111	25	0.5935206657354684
1112	1	0.09088202941645918
1112	2	0.4570654276901571
1112	3	0.2626518075671491
1112	4	0.3108921105971376
1112	5	0.22252870759687937
1112	6	0.187027200819621
1112	7	0.22818070527346634
1112	8	0.18221572588333856
1112	9	0.2900184973382167
1112	10	0.32470469032312366
1112	11	0.180867793391009
1112	12	0.27624556895572994
1112	13	0.3938658690418322
1112	14	0.46397894289670916
1112	15	0.3739118720696908
1112	16	0.3066986688674689
1112	17	0.36878071496490605
1112	18	0.3009881662240801
1112	19	0.30545911936973624
1112	20	0.26096437491042934
1112	21	0.4031629651604618
1112	22	0.2854005940889479
1112	23	0.28884822367567864
1112	24	0.4640173817552551
1112	25	0.4344729940084662
1113	1	-0.2956111865090776
1113	2	-0.3546433119137937
1113	3	-0.17003991007850416
1113	4	0.014398867519012981
1113	5	0.07463724192725607
1113	6	-0.025854530646704177
1113	7	0.09034547261650516
1113	8	-0.0671998146296815
1113	9	-0.05410364586997218
1113	10	-0.0032855564667571002
1113	11	-0.2383622134785952
1113	12	-0.14148018446074673
1113	13	-0.16473023347548832
1113	14	-0.14115018124221734
1113	15	-0.039795841166380796
1113	16	-0.22556376633966124
1113	17	-0.20393962150998846
1113	18	-0.08023690430476904
1113	19	-0.0784116994709052
1113	20	0.08126745211338715
1113	21	-0.09662062870197019
1113	22	-0.24215629737400235
1113	23	-0.11568734485881293
1113	24	-0.10335498071009397
1113	25	0.008710617179396252
1114	1	-0.1610338951579351
1114	2	-0.5412626747060614
1114	3	-0.22085196921950612
1114	4	-0.07666276418574114
1114	5	0.011994751556777317
1114	6	-0.16224922792700625
1114	7	0.0445823211074412
1114	8	0.00336272773044044
1114	9	0.04091076005639849
1114	10	-0.004729217161873578
1114	11	-0.21331960931105698
1114	12	0.10680825760734791
1114	13	0.03855258846837728
1114	14	0.0758083692956717
1114	15	0.03818582300399953
1114	16	-0.19061077735398868
1114	17	-0.16132095042531347
1114	18	-0.014990469527496941
1114	19	-0.10142269148558825
1114	20	-0.05226614172595845
1114	21	-0.03890878627742467
1114	22	0.17160143204002765
1114	23	0.06487901862794843
1114	24	-9.153168576716553E-4
1114	25	0.2756126875606455
1115	1	-0.9662289738971452
1115	2	-1.5065353372034735
1115	3	-0.9934428527066572
1115	4	-0.7639644718468617
1115	5	-0.8766895261174952
1115	6	-0.8863881757920709
1115	7	-0.799083276766531
1115	8	-0.8155310718275999
1115	9	-0.7938602679584861
1115	10	-0.6840450694758999
1115	11	-0.8662731120143334
1115	12	-0.9627860344619208
1115	13	-0.7789973968477568
1115	14	-0.8805366244697055
1115	15	-0.9435875303571635
1115	16	-0.994496946117323
1115	17	-0.8801670876063028
1115	18	-1.0144332048665063
1115	19	-0.9457213805294984
1115	20	-0.8714800976551089
1115	21	-0.9074057604386354
1115	22	-0.7706259897142402
1115	23	-0.766288444818862
1115	24	-0.905473814000338
1115	25	-0.9388403264829037
1116	1	-2.6637242115128466
1116	2	-3.1829890182957605
1116	3	-2.7921460995198473
1116	4	-2.9213235094676016
1116	5	-2.7773927645388135
1116	6	-2.963449637823068
1116	7	-2.944429357345232
1116	8	-2.953105234948705
1116	9	-3.3500679414813064
1116	10	-3.52907351790369
1116	11	-3.5352045336804494
1116	12	-3.5890500593689794
1116	13	-3.7374356285136265
1116	14	-3.8225750508268845
1116	15	-3.9793064615690312
1116	16	-4.345923143846484
1116	17	-4.3129167666174055
1116	18	-3.878189152601955
1116	19	-3.939262790195251
1116	20	-3.755372329304194
1116	21	-4.158122216049861
1116	22	-4.232304530872149
1116	23	-4.46853557689845
1116	24	-4.64854213218635
1116	25	-4.733120010613042
1201	1	0.5482996466879229
1201	2	0.7973746959510137
1201	3	0.4757232125773534
1201	4	0.35963665979841153
1201	5	0.47183002666298535
1201	6	0.5092523228848904
1201	7	0.5151038630575542
1201	8	0.41762498435722506
1201	9	0.6413616683890808
1201	10	0.5184287050108267
1201	11	0.7052953462497129
1201	12	0.5842107128423208
1201	13	0.651136319278713
1201	14	0.7618145725432512
1201	15	0.7333274811998436
1201	16	0.3585460501279627
1201	17	0.5079260587231715
1201	18	0.5793223513863097
1201	19	0.6007302003224808
1201	20	0.5347748049209002
1201	21	0.7303069459924956
1201	22	0.5761678563108816
1201	23	0.6709206690045733
1201	24	0.6035233934923667
1201	25	0.4159937441978627
1202	1	0.5396915574715102
1202	2	0.9359211623900769
1202	3	0.5542571051048952
1202	4	0.4240686718099482
1202	5	0.4241552718409949
1202	6	0.4735731857629375
1202	7	0.49660243987115393
1202	8	0.567125874346111
1202	9	0.6263379663005892
1202	10	0.6923054753724784
1202	11	0.5476174346862237
1202	12	0.6406991429868114
1202	13	0.6376817034064004
1202	14	0.6824875155547829
1202	15	0.7144918147957995
1202	16	0.7761882560270905
1202	17	0.5542890415520887
1202	18	0.6990583522988416
1202	19	0.6910527980819907
1202	20	0.7035716089190913
1202	21	0.6992011049596343
1202	22	0.47712310220512677
1202	23	0.4916694799210646
1202	24	0.6642323074406562
1202	25	0.655016109313479
1203	1	0.46178427514165676
1203	2	0.8038716539129886
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2211	10	0.03199914492176248
2211	11	-0.052349578483131154
2211	12	0.07235538396225394
2211	13	0.0057301517566372695
2211	14	-0.005523523554550991
2211	15	0.03722022443182027
2211	16	-0.05881052962255495
2211	17	0.013733364494370903
2211	18	0.023815512434801178
2211	19	-0.0036637507947077097
2211	20	0.16732838602115407
2211	21	0.0705995894987197
2211	22	0.013061227127344921
2211	23	0.029460546410675192
2211	24	0.21507008835735064
2211	25	0.29685585614861765
2212	1	-0.4618310076777661
2212	2	-1.0684612570576668
2212	3	-0.6123096878103489
2212	4	-0.4443003735233617
2212	5	-0.27689058977800585
2212	6	-0.449097014396294
2212	7	-0.35038404292997427
2212	8	-0.3386787065571326
2212	9	-0.37138368922301623
2212	10	-0.44293683261616223
2212	11	-0.5920604908095868
2212	12	-0.4267633340371262
2212	13	-0.3287142442467115
2212	14	-0.36230769637528226
2212	15	-0.38797498533481445
2212	16	-0.4431485659575287
2212	17	-1.5985489831978512
2212	18	-0.7798746046938874
2212	19	-0.5914955479475381
2212	20	-0.6551208928450052
2212	21	-0.5858334860084398
2212	22	-0.47801809278227125
2212	23	-0.45481877553724104
2212	24	-0.21350020782473678
2212	25	-0.23594804742390352
2213	1	-0.27727935114836555
2213	2	-0.8008804233015496
2213	3	-0.52687150260018
2213	4	-0.45852709169519557
2213	5	-0.5423235297984661
2213	6	-0.359649351197028
2213	7	-0.16788007181745712
2213	8	-0.414881120489639
2213	9	-0.554641799511721
2213	10	-0.3303238692099484
2213	11	-0.5322744899689837
2213	12	-0.3420752796703965
2213	13	-0.3718708177406356
2213	14	-0.6680461400656483
2213	15	-0.6369119182273053
2213	16	-0.6110352927644556
2213	17	-1.4478482693710504
2213	18	-0.9769465748810546
2213	19	-0.8092087139463402
2213	20	-0.672300607016524
2213	21	-0.5122399240779814
2213	22	-0.49902920044493726
2213	23	-0.5173905676399073
2213	24	-0.38435882705717717
2213	25	-0.31280324697588213
2214	1	-0.5837146295269804
2214	2	-1.788657634025462
2214	3	-0.8305720405688568
2214	4	-0.5758384758762105
2214	5	-0.4437001067354842
2214	6	-0.6042449426499914
2214	7	-0.370893060129454
2214	8	-0.3765419012887108
2214	9	-0.6123094728568717
2214	10	-0.3492152877400301
2214	11	-0.5204068986887833
2214	12	-0.47291203521801606
2214	13	-0.4365503836829987
2214	14	-0.3246384443742869
2214	15	-0.40371221511600197
2214	16	-0.46303025520440144
2214	17	-0.4623630802750043
2214	18	-0.49534526275124335
2214	19	-0.4015391651557749
2214	20	-0.4298134708671455
2214	21	-0.5340627206285262
2214	22	-0.4996310479291708
2214	23	-0.5691682057380447
2214	24	-0.6242822861514412
2214	25	-0.6161326740784574
2215	1	-0.928633066291713
2215	2	-2.095036779290183
2215	3	-1.0809511238694682
2215	4	-1.0283897586226018
2215	5	-0.8247567258427111
2215	6	-0.868596336335969
2215	7	-1.0642738039489785
2215	8	-1.0053119554656007
2215	9	-0.9153699635291872
2215	10	-1.0864421913638012
2215	11	-1.0728103612907631
2215	12	-1.0480610916655309
2215	13	-1.003433189894423
2215	14	-1.026722166224559
2215	15	-0.9029016173470623
2215	16	-0.9992348702196168
2215	17	-1.1660197371565033
2215	18	-1.3058471064813375
2215	19	-1.2830036153345503
2215	20	-1.2358544946810213
2215	21	-1.2618377146555417
2215	22	-1.3252813284657137
2215	23	-1.444355215201277
2215	24	-1.3407756419904544
2215	25	-1.2247232825838665
2216	1	-3.1264508901315793
2216	2	-4.6104186601182064
2216	3	-3.1709863996495002
2216	4	-2.880669011541741
2216	5	-3.062526421486563
2216	6	-2.8717000580674927
2216	7	-3.166341128732263
2216	8	-3.0172619677711054
2216	9	-3.5974914325329834
2216	10	-3.4558671141125785
2216	11	-3.395816185896301
2216	12	-3.333876946624649
2216	13	-3.420165941678725
2216	14	-3.970280918172932
2216	15	-3.879151993348593
2216	16	-3.609560555909681
2216	17	-3.6294997338750363
2216	18	-3.8037141523398503
2216	19	-4.100608690671585
2216	20	-4.3335465289176724
2216	21	-4.466232147098321
2216	22	-4.5292382318538245
2216	23	-4.561995839774003
2216	24	-4.406897220376521
2216	25	-4.285471457267338
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	13510.0
3	0.0
4	218.0
5	1947.0
6	2782.0
7	1890.0
8	1431.0
9	1161.0
10	1107.0
11	1054.0
12	950.0
13	898.0
14	821.0
15	862.0
16	933.0
17	1004.0
18	1203.0
19	1488.0
20	1940.0
21	2422.0
22	3041.0
23	3568.0
24	4404.0
25	5036.0
26	5698.0
27	6694.0
28	7534.0
29	9315.0
30	12011.0
31	15702.0
32	20533.0
33	27559.0
34	38032.0
35	54434.0
36	82094.0
37	149182.0
38	312429.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.56397249123656	16.25237198823645	14.194128809356407	23.989526711170583
2	11.926623973025	22.08238446924422	37.57036272819919	28.420628829531587
3	20.649979179430535	28.451088016284075	27.908117757618378	22.99081504666701
4	13.929150190406341	15.847201114967124	32.898586885022794	37.32506180960374
5	15.962815939909431	34.32842395668405	30.8963493066376	18.812410796768926
6	36.02863713084948	31.977500116426448	14.523601844194978	17.470260908529088
7	26.090491268518846	31.985436624397806	22.4346712039829	19.489400903100442
8	26.24393963606988	33.77231780529575	23.183337884242487	16.800404674391885
9	27.740666690992636	19.16538431050341	16.176435431532248	36.91751356697171
10	14.096916853819492	26.523109441938587	30.740244462478216	28.6397292417637
11	32.859053613153336	24.717363595298643	18.68111818951644	23.742464602031585
12	25.666748872604284	23.986853760669995	26.472660653889513	23.873736712836205
13	31.94791306262385	19.485169691441513	26.41663259208002	22.15028465385462
14	29.615104917628525	19.600686449911553	23.425606494097988	27.35860213836193
15	32.06780940399434	20.94920584997641	25.413429784557316	21.56955496147193
16	27.20673708364994	22.28677391921337	29.70936639811595	20.79712259902074
17	24.530027538505472	28.100220534491065	27.49434825327374	19.875403673729725
18	27.02031861132463	19.454337534769092	34.70908443590095	18.816259418005327
19	24.77282934555478	32.20118079676734	20.86120417115892	22.164785686518965
20	20.967760197361386	27.139203433947216	29.12778797489454	22.765248393796856
21	22.53835295538552	30.74954645758844	22.0573841233736	24.654716463652445
22	20.821150526649525	29.062976513393956	20.2219074123467	29.89396554760982
23	23.463482630126233	24.503133798475748	20.958330922073966	31.075052649324054
24	19.783537747539544	28.718675813620543	21.62773172569706	29.870054713142853
25	26.441159578005504	26.676566609231884	21.12000342593432	25.76227038682829
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	335.0
1	335.0
2	422.5
3	510.0
4	510.0
5	510.0
6	1197.5
7	1885.0
8	1885.0
9	1885.0
10	1900.5
11	1916.0
12	1916.0
13	1916.0
14	2069.0
15	2222.0
16	2222.0
17	2222.0
18	3643.5
19	5065.0
20	5065.0
21	5065.0
22	7566.5
23	10068.0
24	10068.0
25	10068.0
26	14790.5
27	19513.0
28	19513.0
29	19513.0
30	22939.5
31	26366.0
32	26366.0
33	26366.0
34	30774.0
35	35182.0
36	35182.0
37	35182.0
38	38534.5
39	41887.0
40	41887.0
41	41887.0
42	50301.5
43	58716.0
44	58716.0
45	58716.0
46	105238.0
47	151760.0
48	151760.0
49	151760.0
50	197610.0
51	243460.0
52	243460.0
53	243460.0
54	159217.0
55	74974.0
56	74974.0
57	74974.0
58	57956.5
59	40939.0
60	40939.0
61	40939.0
62	35646.5
63	30354.0
64	30354.0
65	30354.0
66	25467.0
67	20580.0
68	20580.0
69	20580.0
70	15966.5
71	11353.0
72	11353.0
73	11353.0
74	9425.5
75	7498.0
76	7498.0
77	7498.0
78	6613.0
79	5728.0
80	5728.0
81	5728.0
82	4269.0
83	2810.0
84	2810.0
85	2810.0
86	2025.5
87	1241.0
88	1241.0
89	1241.0
90	860.0
91	479.0
92	479.0
93	479.0
94	258.0
95	37.0
96	37.0
97	37.0
98	23.0
99	9.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15712925233397956
2	0.009686911472951502
3	0.0
4	0.16618714358141473
5	0.04440882792145298
6	0.04956679377068691
7	0.03660897712505048
8	0.021889903847968326
9	0.015851309683011548
10	0.010315931698467833
11	0.017360958224250742
12	0.016983546088940944
13	0.007799850796402507
14	0.009183695292538437
15	0.001509648541239195
16	3.774121353097988E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.004151533488407786
22	0.006416006300266578
23	6.290202255163313E-4
24	0.0017612566314457274
25	0.11863321453238007
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	794887.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.827958287348814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.02764979989972	32.78706573251405
2	6.596119352511747	4.858432167453538
3	1.4629085421827641	1.6162780430913928
4	0.6126884475688538	0.90256258360825
5	0.37571306967557105	0.6918372629011855
6	0.257201767451778	0.5683329577887881
7	0.19004212848038768	0.4899204506360291
8	0.1417755460115087	0.4177043115742356
9	0.11832852684389843	0.3922018245729473
>10	0.8895190160361851	6.746849105565535
>50	0.14172198831542676	3.7177310721312447
>100	0.14995764855373042	10.891721133044944
>500	0.017843930720352362	4.662644905768907
>1k	0.014069253067970132	11.036042598890942
>5k	0.0024020675969705103	6.850972841486332
>10k+	0.0020589150831175802	13.369703008971676
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	28591	3.5968634535474853	No Hit
CTTCCGTACTCTGCGTTGATACCAC	20771	2.6130758208399434	No Hit
TCCGTACTCTGCGTTGATACCACTG	17115	2.153136231942402	No Hit
GAGTACGGAAGCAGTGGTATCAACG	16092	2.0244386938017604	No Hit
GCTTCCGTACTCTGCGTTGATACCA	13065	1.6436298492741734	No Hit
CCCATGTACTCTGCGTTGATACCAC	10159	1.2780432942040818	No Hit
GTACATGGGAAGCAGTGGTATCAAC	9894	1.2447052222517163	No Hit
TCCATGTACTCTGCGTTGATACCAC	8951	1.1260720077193362	No Hit
GTACATGGAAGCAGTGGTATCAACG	8637	1.0865695375569104	No Hit
GCGTTGATACCACTGCTTCCGTACT	8045	1.012093542855777	No Hit
GTATCAACGCAGAGTACGGAAGCAG	6825	0.8586126078297921	No Hit
TATCAACGCAGAGTACGGAAGCAGT	6008	0.7558307029804237	No Hit
ACTCTGCGTTGATACCACTGCTTCC	5851	0.7360794678992109	No Hit
CATGTACTCTGCGTTGATACCACTG	4395	0.5529087782288552	No Hit
CAGTGGTATCAACGCAGAGTACGGA	4204	0.5288802056141313	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	3965	0.4988130388344507	No Hit
GGTATCAACGCAGAGTACGGAAGCA	3701	0.4656007709271884	No Hit
ATACCACTGCTTCCGTACTCTGCGT	3642	0.45817833226609567	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3499	0.4401883538163286	No Hit
GAGTACATGGAAGCAGTGGTATCAA	3144	0.3955279178046691	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	3098	0.38974093172991886	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	2888	0.3633220822582329	No Hit
GTGGTATCAACGCAGAGTACGGAAG	2714	0.34143217841026463	No Hit
GTATCAACGCAGAGTACATGGGAAG	2548	0.3205487069231224	No Hit
GCGTTGATACCACTGCTTCCCATGT	2369	0.29802978284963777	No Hit
CATGGAAGCAGTGGTATCAACGCAG	2321	0.291991188684681	No Hit
TATCAACGCAGAGTACATGGGAAGC	2287	0.28771385115116993	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	2068	0.26016276527355464	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	2063	0.2595337450480383	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2000	0.2516080902065325	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1971	0.24795977289853777	No Hit
GTTGATACCACTGCTTCCGTACTCT	1957	0.24619851626709205	No Hit
GTACTCTGCGTTGATACCACTGCTT	1931	0.24292761109440714	No Hit
GATACCACTGCTTCCGTACTCTGCG	1920	0.2415437665982712	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1916	0.24104055041785813	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	1882	0.23676321288434707	No Hit
CAGTGGTATCAACGCAGAGTACATG	1878	0.236259996703934	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	1738	0.21864743038947673	No Hit
GCGTTGATACCACTGCTTCCATGTA	1700	0.2138668766755526	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	1551	0.19512207395516595	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1494	0.18795124338427976	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1451	0.18254166944483935	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1399	0.1759998590994695	No Hit
GTATCAACGCAGAGTACATGGAAGC	1357	0.17071608920513232	No Hit
CCATGTACTCTGCGTTGATACCACT	1324	0.16656455571672452	No Hit
GGTATCAACGCAGAGTACATGGGAA	1318	0.16580973144610492	No Hit
CCCGTACTCTGCGTTGATACCACTG	1287	0.16190980604790367	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	1268	0.15951952919094162	No Hit
TATCAACGCAGAGTACATGGAAGCA	1265	0.1591421170556318	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	1227	0.1543615633417077	No Hit
GCAGTGGTATCAACGCAGAGTACGG	1222	0.15373254311619136	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	1142	0.14366821950793005	No Hit
GTACGGGAAGCAGTGGTATCAACGC	1138	0.143165003327517	No Hit
TGATACCACTGCTTCCGTACTCTGC	1085	0.13649738893704388	No Hit
CATGGGAAGCAGTGGTATCAACGCA	984	0.12379118038161399	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	977	0.12291055206589113	No Hit
TACCACTGCTTCCGTACTCTGCGTT	956	0.12026866711872253	No Hit
AAAGTACTCTGCGTTGATACCACTG	949	0.11938803880299967	No Hit
ATACCACTGCTTCCCATGTACTCTG	928	0.11674615385583109	No Hit
TTCCCGTACTCTGCGTTGATACCAC	918	0.11548811340479842	No Hit
GGTATCAACGCAGAGTACTTTTTTT	907	0.11410426890866249	No Hit
CTGCGTTGATACCACTGCTTCCGTA	897	0.11284622845762983	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	878	0.11045595160066776	No Hit
ATACCACTGCTTCCATGTACTCTGC	832	0.10466896552591752	No Hit
GCAGTGGTATCAACGCAGAGTACAT	821	0.10328512102978159	No Hit
TACCACTGCTTCCCATGTACTCTGC	815	0.10253029675916199	No Hit
CCCCATGTACTCTGCGTTGATACCA	803	0.1010206482179228	No Hit
GTGGTATCAACGCAGAGTACATGGG	803	0.1010206482179228	No Hit
TACGGAAGCAGTGGTATCAACGCAG	803	0.1010206482179228	No Hit
TCAACGCAGAGTACGGAAGCAGTGG	802	0.10089484417281955	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	1.2580404510326626E-4	0.0
7	0.0	0.0	0.0	1.2580404510326626E-4	0.0
8	0.0	0.0	0.0	1.2580404510326626E-4	0.0
9	0.0	0.0	0.0	1.2580404510326626E-4	0.0
10	0.0	0.0	0.0	2.516080902065325E-4	0.0
11	0.0	0.0	0.0	5.03216180413065E-4	0.0
12	0.0	0.0	0.0	8.806283157228637E-4	0.0
13	0.0	0.0	0.0	0.0011322364059293962	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGAA	40	2.6911582E-4	16.679325	1
TTTAAGG	50	8.585791E-5	15.224668	2
CGACCAT	90	2.3028406E-9	14.768236	10
GACCATA	90	2.3028406E-9	14.768236	11
TTTTCGG	110	1.2732926E-11	14.670492	16
TTCGGAA	105	8.913048E-11	14.465024	18
CTCTTGA	40	0.005163108	14.296564	1
TCCTTAT	40	0.005163108	14.296564	1
CCCTATA	40	0.0052196323	14.273126	2
CGAGGTT	40	0.0052218167	14.272225	4
CTCGTAG	40	0.005298756	14.240799	10
GCTCGTA	40	0.005298756	14.240799	9
CGGTCCA	60	2.5808165E-5	14.240798	10
CCGACCA	95	4.9312803E-9	13.99096	9
AGCCCGT	55	1.9047911E-4	13.863336	1
AAAGCTC	55	1.9637648E-4	13.813605	6
GTTTTCG	120	5.2750693E-11	13.447951	15
CGCCAGT	120	5.2750693E-11	13.447951	18
GCACGCC	85	2.7188253E-7	13.401419	15
ATACAGT	50	0.0015021672	13.295595	6
>>END_MODULE