FastQCFastQC Report
Thu 2 Feb 2017
SRR4050283_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050283_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9753336
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA201580.20667800227532404No Hit
GAATAGGACCGCGGTTCTATTTTGT131560.13488718116550072No Hit
GATTAAGAGGGACGGCCGGGGGCAT128810.13206763306421515No Hit
GTATCTGATCGTCTTCGAACCTCCG128250.13149347054177155No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG125070.1282330476464668No Hit
CTATTGGAGCTGGAATTACCGCGGC114090.1169753610456976No Hit
GTATCAACGCAGAGTACTTTTTTTT112440.11528363218492627No Hit
GTATCAACGCAGAGTACGGGCGGTG108250.11098766616878573No Hit
GTCCTATTCCATTATTCCTAGCTGC105350.10801432453470278No Hit
ATCAGATACCGTCGTAGTTCCGACC100820.10336975984422152No Hit
GAACTACGACGGTATCTGATCGTCT100280.10281610312615089No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC99430.10194460644029898No Hit
GAATAATTGCAATCCCCGATCCCCA98330.10081678719978478No Hit
CTTATGACCCGCACTTACTGGGAAT98060.10053995884074945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54150.015.366521
GTATCAA181850.013.5293861
CTCGACG4700.012.74586413
ACGCTTA4800.012.28133717
CGACCAT18600.012.22002410
TCGACGC5150.011.63184614
GCGCGCT10400.011.52000215
CGCTTAC5300.011.48163618
CCGACCA20900.011.4212739
CGTCGTA15850.011.40017210
CGAACGA10050.011.25884916
CGGTTCT24800.010.96524912
CGGTCCA18350.010.9353710
AACCGCG11300.010.6875647
GTGCGAG5250.010.68543918
GACCATA21600.010.4348511
GTCGTAG17250.010.36478611
GCGTTAT8800.010.3269881
ACGGTAT21150.010.162149
CGCGCTA11700.010.1586816
Produced by FastQC (version 0.11.5)