##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050283_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9753336 Sequences flagged as poor quality 0 Sequence length 25 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.523582085145023 31.0 31.0 34.0 27.0 34.0 2 30.67185186688944 31.0 31.0 34.0 27.0 34.0 3 30.778644660657644 31.0 31.0 34.0 27.0 34.0 4 34.318859413845686 37.0 35.0 37.0 31.0 37.0 5 34.34020882701057 37.0 35.0 37.0 31.0 37.0 6 34.37257365069757 37.0 35.0 37.0 31.0 37.0 7 34.283948794545786 37.0 35.0 37.0 30.0 37.0 8 34.278725556055896 37.0 35.0 37.0 30.0 37.0 9 35.87289190078143 39.0 37.0 39.0 31.0 39.0 10 35.75376548085701 39.0 37.0 39.0 30.0 39.0 11 35.63711277864312 39.0 37.0 39.0 30.0 39.0 12 35.565140378635576 39.0 37.0 39.0 30.0 39.0 13 35.56548159522034 39.0 37.0 39.0 30.0 39.0 14 36.740793406481636 40.0 37.0 41.0 30.0 41.0 15 36.69261009771426 40.0 37.0 41.0 30.0 41.0 16 36.693841368737836 40.0 37.0 41.0 30.0 41.0 17 36.661817351519524 40.0 37.0 41.0 30.0 41.0 18 36.625989815177086 40.0 37.0 41.0 30.0 41.0 19 36.63911865642689 40.0 37.0 41.0 30.0 41.0 20 36.59953086820755 40.0 37.0 41.0 30.0 41.0 21 36.5458190920522 40.0 37.0 41.0 30.0 41.0 22 36.46425581975234 40.0 37.0 41.0 30.0 41.0 23 36.3835774754402 40.0 37.0 41.0 30.0 41.0 24 36.31468781553306 40.0 37.0 41.0 30.0 41.0 25 36.08148842611389 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5581517340901705 1101 2 0.5744369388471249 1101 3 0.5793107820334562 1101 4 0.5713853899893806 1101 5 0.5929298875793094 1101 6 0.5957586807981272 1101 7 0.5686469326448602 1101 8 0.5584699186222437 1101 9 0.6402193866770745 1101 10 0.6641473084433756 1101 11 0.5704884780019626 1101 12 0.5817680404714096 1101 13 0.5255170034279004 1101 14 0.6555671299142247 1101 15 0.6831065857418537 1101 16 0.7057724798007357 1101 17 0.6865606098769135 1101 18 0.7187498643866803 1101 19 0.7277865754036057 1101 20 0.7549045713181783 1101 21 0.7643457054135894 1101 22 0.7570359703922094 1101 23 0.7311317145997975 1101 24 0.824858203693978 1101 25 0.8376898239069419 1102 1 0.479245498540287 1102 2 0.532162540403128 1102 3 0.5196513615613796 1102 4 0.5388475554974761 1102 5 0.5088880981332125 1102 6 0.5122783429296334 1102 7 0.506700806517344 1102 8 0.5263574851174511 1102 9 0.6287917501500502 1102 10 0.6574654870592127 1102 11 0.5908677664323321 1102 12 0.5282828104759787 1102 13 0.5703766696285584 1102 14 0.6972769438658446 1102 15 0.666062376002202 1102 16 0.6877959655430175 1102 17 0.6817958050102249 1102 18 0.7537661202137471 1102 19 0.7755434032354458 1102 20 0.7551757944517448 1102 21 0.7537391055727412 1102 22 0.7765257604152254 1102 23 0.795775831068994 1102 24 0.7274712572353366 1102 25 0.8502170044104247 1103 1 0.3752677033007963 1103 2 0.36507780972041814 1103 3 0.35725118300713277 1103 4 0.3965482159529188 1103 5 0.434454506461023 1103 6 0.3657993938407813 1103 7 0.37599504430856 1103 8 0.4316883251055472 1103 9 0.40615052031037635 1103 10 0.4361177330328445 1103 11 0.36631352147697527 1103 12 0.3529825757192455 1103 13 0.3391272302803543 1103 14 0.4528485208692459 1103 15 0.417727092295614 1103 16 0.4469245616221045 1103 17 0.32405583027794904 1103 18 0.38728090809768645 1103 19 0.47125002078782785 1103 20 0.4751899244025424 1103 21 0.38348424877719367 1103 22 0.45195149980941807 1103 23 0.4571683874031862 1103 24 0.5006310988838791 1103 25 0.5166973905985657 1104 1 0.49767177576247335 1104 2 0.5529259342498456 1104 3 0.500969527462928 1104 4 0.5214478025411466 1104 5 0.5491242513195616 1104 6 0.49224025836938523 1104 7 0.4912714195647254 1104 8 0.4922389957139828 1104 9 0.5795750054724138 1104 10 0.5434638695496332 1104 11 0.503419970068272 1104 12 0.48604689662322187 1104 13 0.48526317892402204 1104 14 0.5253663951976009 1104 15 0.43350405249385915 1104 16 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-0.9240620369795138 2215 19 -0.9428027196308264 2215 20 -0.9314948394456763 2215 21 -0.8257787657057492 2215 22 -0.9580121471426892 2215 23 -0.8345622922081759 2215 24 -0.9514752495251528 2215 25 -0.9103223874320108 2216 1 -0.4621008380353402 2216 2 -0.6367946497129182 2216 3 -0.6289382036812405 2216 4 -0.5257596742378965 2216 5 -0.5517537770781189 2216 6 -0.5287565324814523 2216 7 -0.5718868898180034 2216 8 -0.49992823207178105 2216 9 -0.5327440269694463 2216 10 -0.6175862791402835 2216 11 -0.493248809105431 2216 12 -0.5227494015198317 2216 13 -0.5296098097066704 2216 14 -0.5321974021825611 2216 15 -0.5035109684118169 2216 16 -0.704516370606818 2216 17 -0.7453472468736067 2216 18 -0.7243593414956564 2216 19 -0.688763350554737 2216 20 -0.6306292406075471 2216 21 -0.6848845783480257 2216 22 -0.700789265496617 2216 23 -0.8655195235501765 2216 24 -0.9551183574159694 2216 25 -0.9133821188797668 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 246856.0 3 20.0 4 2089.0 5 11992.0 6 15985.0 7 16382.0 8 13561.0 9 13027.0 10 13564.0 11 16081.0 12 26490.0 13 22946.0 14 10682.0 15 10734.0 16 11343.0 17 12264.0 18 13418.0 19 16012.0 20 20204.0 21 25678.0 22 31876.0 23 38662.0 24 45568.0 25 53092.0 26 60486.0 27 68937.0 28 80493.0 29 98052.0 30 123661.0 31 160801.0 32 208768.0 33 277747.0 34 383167.0 35 542665.0 36 813580.0 37 1522375.0 38 4724078.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.22473494808211 17.342203033835137 12.615532818631136 25.817529199451624 2 17.142989179996547 20.383988941742867 37.03358760752224 25.439434270738342 3 18.68802151795323 23.94693969192631 28.121784565338455 29.243254224782007 4 13.237855711686361 16.250484883948367 36.50697411048451 34.00468529388077 5 16.037446587161114 34.82841175262728 32.64975239986059 16.484389260351016 6 33.19892692385077 36.190598138276286 16.07867966530547 14.531795272567477 7 29.66969644712325 30.36392552938404 20.985636643214857 18.98074138027785 8 27.24924605876166 31.67650924693694 19.779605812559684 21.294638881741715 9 27.727406664445077 14.746672948407102 18.61501166335343 38.91090872379439 10 16.90023448095004 25.52507870922413 30.118428577496598 27.456258232329233 11 36.73907422521668 21.947643680629675 21.893999910783027 19.41928218337062 12 25.48153695885378 23.611797362612908 27.592681110960527 23.31398456757279 13 29.56258228127695 20.062589248252966 24.332891403422487 26.0419370670476 14 24.39162270895387 20.076806697605136 24.450827054569356 31.080743538871637 15 25.35094370470957 26.418643259203417 22.43120727194207 25.79920576414494 16 25.727116044071703 26.07501900078233 23.03363450721679 25.164230447929185 17 25.338448652506646 25.002619072316847 24.05532512879901 25.6036071463775 18 26.88027116183444 23.746355999623844 24.813101916191165 24.560270922350547 19 27.447519243528962 24.369328769029345 24.076283772429452 24.106868215012245 20 26.55645332031022 24.20147505113089 23.968894417724147 25.273177210834742 21 26.950215233534525 24.33050325611294 23.90130572740223 24.8179757829503 22 26.021393835144536 24.13953730812562 24.577642838453166 25.261426018276673 23 24.84575386585437 23.605592655630076 24.840232543643214 26.70842093487234 24 25.142038637234144 25.105862611401257 24.419250930229605 25.332847821134997 25 25.238573474850725 24.301463367612506 24.546918099776015 25.913045057760765 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 5521.0 1 5521.0 2 5285.0 3 5049.0 4 5049.0 5 5049.0 6 8831.5 7 12614.0 8 12614.0 9 12614.0 10 17677.0 11 22740.0 12 22740.0 13 22740.0 14 32025.5 15 41311.0 16 41311.0 17 41311.0 18 65626.5 19 89942.0 20 89942.0 21 89942.0 22 129782.5 23 169623.0 24 169623.0 25 169623.0 26 234255.5 27 298888.0 28 298888.0 29 298888.0 30 360479.0 31 422070.0 32 422070.0 33 422070.0 34 525712.0 35 629354.0 36 629354.0 37 629354.0 38 707235.0 39 785116.0 40 785116.0 41 785116.0 42 887418.0 43 989720.0 44 989720.0 45 989720.0 46 1062009.0 47 1134298.0 48 1134298.0 49 1134298.0 50 1165042.5 51 1195787.0 52 1195787.0 53 1195787.0 54 1117968.0 55 1040149.0 56 1040149.0 57 1040149.0 58 980243.0 59 920337.0 60 920337.0 61 920337.0 62 821377.5 63 722418.0 64 722418.0 65 722418.0 66 615103.5 67 507789.0 68 507789.0 69 507789.0 70 404403.0 71 301017.0 72 301017.0 73 301017.0 74 250218.5 75 199420.0 76 199420.0 77 199420.0 78 168394.5 79 137369.0 80 137369.0 81 137369.0 82 105005.5 83 72642.0 84 72642.0 85 72642.0 86 53725.5 87 34809.0 88 34809.0 89 34809.0 90 24339.5 91 13870.0 92 13870.0 93 13870.0 94 7544.5 95 1219.0 96 1219.0 97 1219.0 98 741.5 99 264.0 100 264.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14468895565578793 2 0.16215990098157185 3 0.17526310997590977 4 0.1952972808483169 5 0.18540323023835126 6 0.06948391811786245 7 0.05685234262410318 8 0.06041009968281622 9 0.06734106156088542 10 0.06871495045387548 11 0.7082704830429302 12 0.6667974936985663 13 0.6640907275213321 14 0.664982730011557 15 0.6667462291876339 16 0.6723545666836455 17 0.6642957855650621 18 0.6750203212521336 19 0.6705808146053822 20 0.6761071288839019 21 0.6642855326628756 22 0.6667564820898203 23 0.6523716603221708 24 0.6623272283452555 25 0.6559089115765108 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 9753336.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.815346202018894 #Duplication Level Percentage of deduplicated Percentage of total 1 81.76967758509605 36.64536409803544 2 11.720271302130286 10.50496031973111 3 2.806849347463872 3.773697757305128 4 1.0730453538030607 1.9235559608460808 5 0.5848129452810732 1.3104297303096812 6 0.34746435194667996 0.9343041135210362 7 0.2506087186933387 0.7861781542640436 8 0.17328156745818077 0.621253874885349 9 0.13248201088556857 0.5343504465038753 >10 0.9077893089704301 7.88303978947737 >50 0.10781116347271878 3.3849748044227543 >100 0.09623083477559247 8.79497707702539 >500 0.01451658103832364 4.459582322675491 >1k 0.013456712402189187 12.629681131251186 >5k 0.0014491843875114165 4.4152561006980475 >10k+ 2.530321946448505E-4 1.3983943190481634 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAAGGGCAGGGACTTAATCAA 20158 0.20667800227532404 No Hit GAATAGGACCGCGGTTCTATTTTGT 13156 0.13488718116550072 No Hit GATTAAGAGGGACGGCCGGGGGCAT 12881 0.13206763306421515 No Hit GTATCTGATCGTCTTCGAACCTCCG 12825 0.13149347054177155 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 12507 0.1282330476464668 No Hit CTATTGGAGCTGGAATTACCGCGGC 11409 0.1169753610456976 No Hit GTATCAACGCAGAGTACTTTTTTTT 11244 0.11528363218492627 No Hit GTATCAACGCAGAGTACGGGCGGTG 10825 0.11098766616878573 No Hit GTCCTATTCCATTATTCCTAGCTGC 10535 0.10801432453470278 No Hit ATCAGATACCGTCGTAGTTCCGACC 10082 0.10336975984422152 No Hit GAACTACGACGGTATCTGATCGTCT 10028 0.10281610312615089 No Hit GTGTAGCGCGCGTGCAGCCCCGGAC 9943 0.10194460644029898 No Hit GAATAATTGCAATCCCCGATCCCCA 9833 0.10081678719978478 No Hit CTTATGACCCGCACTTACTGGGAAT 9806 0.10053995884074945 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.1264510932464546E-5 2 0.0 0.0 0.0 0.0 5.1264510932464546E-5 3 0.0 0.0 0.0 1.025290218649291E-5 5.1264510932464546E-5 4 0.0 0.0 0.0 3.075870655947872E-5 5.1264510932464546E-5 5 0.0 0.0 0.0 5.1264510932464546E-5 5.1264510932464546E-5 6 0.0 0.0 0.0 5.1264510932464546E-5 6.151741311895745E-5 7 0.0 0.0 0.0 5.1264510932464546E-5 6.151741311895745E-5 8 0.0 0.0 0.0 5.1264510932464546E-5 6.151741311895745E-5 9 0.0 0.0 0.0 6.151741311895745E-5 6.151741311895745E-5 10 1.025290218649291E-5 0.0 0.0 8.202321749194328E-5 6.151741311895745E-5 11 1.025290218649291E-5 0.0 0.0 8.202321749194328E-5 6.151741311895745E-5 12 1.025290218649291E-5 1.025290218649291E-5 0.0 8.202321749194328E-5 1.0252902186492909E-4 13 1.025290218649291E-5 2.050580437298582E-5 0.0 9.227611967843618E-5 1.12781924051422E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5415 0.0 15.36652 1 GTATCAA 18185 0.0 13.529386 1 CTCGACG 470 0.0 12.745864 13 ACGCTTA 480 0.0 12.281337 17 CGACCAT 1860 0.0 12.220024 10 TCGACGC 515 0.0 11.631846 14 GCGCGCT 1040 0.0 11.520002 15 CGCTTAC 530 0.0 11.481636 18 CCGACCA 2090 0.0 11.421273 9 CGTCGTA 1585 0.0 11.400172 10 CGAACGA 1005 0.0 11.258849 16 CGGTTCT 2480 0.0 10.965249 12 CGGTCCA 1835 0.0 10.93537 10 AACCGCG 1130 0.0 10.687564 7 GTGCGAG 525 0.0 10.685439 18 GACCATA 2160 0.0 10.43485 11 GTCGTAG 1725 0.0 10.364786 11 GCGTTAT 880 0.0 10.326988 1 ACGGTAT 2115 0.0 10.16214 9 CGCGCTA 1170 0.0 10.15868 16 >>END_MODULE