FastQCFastQC Report
Thu 2 Feb 2017
SRR4050283_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050283_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9753336
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA198520.20354061420625721No Hit
GTATCAACGCAGAGTACTTTTTTTT179330.18386529491037734No Hit
GAATAGGACCGCGGTTCTATTTTGT139380.14290495067533815No Hit
GATTAAGAGGGACGGCCGGGGGCAT137470.14094664635771803No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG126360.1295556720285244No Hit
CTATTGGAGCTGGAATTACCGCGGC116220.11915922921142058No Hit
GTATCTGATCGTCTTCGAACCTCCG114780.11768281129656562No Hit
ATCAGATACCGTCGTAGTTCCGACC112850.11570400117457247No Hit
TCGTAGTTCCGACCATAAACGATGC107530.11024945721135825No Hit
TATCAACGCAGAGTACTTTTTTTTT106840.10954200696049023No Hit
GTATCAACGCAGAGTACGGGCGGTG102710.10530755835746868No Hit
CTTATGACCCGCACTTACTGGGAAT101210.10376962302949472No Hit
GAACTACGACGGTATCTGATCGTCT101070.10362608239888384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57400.015.3716211
GTATCAA191700.013.5249861
CTCGACG5250.011.93283513
ACGCTTA5250.011.75179317
AACCGCG12050.011.3483487
CGCTTAC5500.010.87246318
AGAACCG14150.010.8111475
CGTCGTA20650.010.75968510
GCGTTAT8350.010.7150261
CGAGCCG23700.010.6934415
CCGACCA18900.010.5003529
CGACCAT17950.010.47380510
AGGCCCG24300.010.47206410
ATACCGT22300.010.4367556
CGGTTCT27350.010.41402612
CGAACGA10400.010.4046716
TCGACGC5800.010.310214
AAAGGGC43350.010.2579155
GAGCGTT5000.010.2560697
TACCGTC22800.010.1627727
Produced by FastQC (version 0.11.5)