FastQCFastQC Report
Thu 2 Feb 2017
SRR4050282_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050282_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442754
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC106090.7353297928822239No Hit
CCCATGTACTCTGCGTTGATACCAC92920.6440460397268003No Hit
GTACGGAAGCAGTGGTATCAACGCA55540.38495821186425405No Hit
GAGTACATGGGAAGCAGTGGTATCA36770.254859802849273No Hit
CTTCCGTACTCTGCGTTGATACCAC35890.2487603569284854No Hit
GAGTACGGAAGCAGTGGTATCAACG27940.19365740798500644No Hit
GCGTTGATACCACTGCTTCCCATGT27870.19317222478676196No Hit
GTATCAACGCAGAGTACATGGGAAG27340.18949869485719673No Hit
CATGTACTCTGCGTTGATACCACTG26540.18395374402011708No Hit
TCCGTACTCTGCGTTGATACCACTG26280.1821516349980662No Hit
GTATCAACGCAGAGTACTTTTTTTT22540.1562289898347189No Hit
GCTTCCGTACTCTGCGTTGATACCA21740.15068403899763924No Hit
GTATCTGATCGTCTTCGAACCTCCG21080.14610945455704852No Hit
ACTCTGCGTTGATACCACTGCTTCC20960.1452777119314866No Hit
TATCAACGCAGAGTACATGGGAAGC20640.14305973159665472No Hit
CTATTGGAGCTGGAATTACCGCGGC19870.13772271641596556No Hit
GAATAGGACCGCGGTTCTATTTTGT19860.1376534045305021No Hit
GATTAAGAGGGACGGCCGGGGGCAT19480.13501955288288925No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG19340.13404918648640032No Hit
GTCCTATTCCATTATTCCTAGCTGC19150.13273226066259391No Hit
CTTTAATATACGCTATTGGAGCTGG17370.1203947450500917No Hit
CTCTTAATCATGGCCTCAGTTCCGA17250.11956300242452975No Hit
GAACTACGACGGTATCTGATCGTCT16770.11623603192228198No Hit
ACGCAGAGTACATGGGAAGCAGTGG16540.11464185855662157No Hit
GAATAACGCCGCCGCATCGCCAGTC16140.11186938313808176No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15720.10895828394861494No Hit
GGTATCAACGCAGAGTACATGGGAA15100.10466094704987823No Hit
GTCCTGTATTGTTATTTTTCGTCAC15020.10410645196617026No Hit
ATACAGGACTCTTTCGAGGCCCTGT15000.10396782819524326No Hit
GCGTTGATACCACTGCTTCCGTACT15000.10396782819524326No Hit
ATATTAAAGTTGCTGCAGTTAAAAA14710.1019577835168019No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTA400.005230183214.2699048
CCGCTAC400.005230183214.2699049
CGAACGA1203.6379788E-1214.25399716
GTCATAC500.001524397513.2709091
GTACTAG500.001524397513.2709091
CCAACGT751.4741399E-512.6702219
ATCGCCA2700.012.31826916
CGGAATT853.927318E-612.29842315
CGCGGTA550.003061811412.09430117
ATACCGT2700.011.9796726
GGTTCTA3050.011.84103613
AAGCTTA1850.011.81494715
AAGCTCG1052.6702946E-711.7783347
GGACTAT657.929367E-411.7086386
TACCGTC2550.011.5651517
ACGGTAT3800.011.5160639
GCATCGC2900.011.46953214
TAGAGTG2500.011.4159245
ACCGTCG2500.011.4159248
CCGGTAG752.0468935E-411.4159239
Produced by FastQC (version 0.11.5)