##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050282_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1442754
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.505789621792765	31.0	31.0	34.0	27.0	34.0
2	30.65546032102493	31.0	31.0	34.0	27.0	34.0
3	30.748708373014388	31.0	31.0	34.0	27.0	34.0
4	34.34194880069644	37.0	35.0	37.0	30.0	37.0
5	34.35084082248256	37.0	35.0	37.0	30.0	37.0
6	34.33250436318319	37.0	35.0	37.0	30.0	37.0
7	34.22997822220559	37.0	35.0	37.0	30.0	37.0
8	34.19267387233028	37.0	35.0	37.0	30.0	37.0
9	35.754055091859044	39.0	37.0	39.0	30.0	39.0
10	35.624228385435075	39.0	37.0	39.0	30.0	39.0
11	35.476226716404874	39.0	37.0	39.0	30.0	39.0
12	35.398477495123906	39.0	37.0	39.0	30.0	39.0
13	35.44622991861399	39.0	37.0	39.0	30.0	39.0
14	36.61457046731459	40.0	37.0	41.0	30.0	41.0
15	36.578559477222036	40.0	37.0	41.0	30.0	41.0
16	36.5730214575735	40.0	37.0	41.0	30.0	41.0
17	36.53291898688203	40.0	37.0	41.0	30.0	41.0
18	36.52297342443688	40.0	37.0	41.0	30.0	41.0
19	36.512135818025804	40.0	37.0	41.0	30.0	41.0
20	36.45979009588606	40.0	37.0	41.0	30.0	41.0
21	36.37377335290701	40.0	37.0	41.0	30.0	41.0
22	36.31205458449604	40.0	37.0	41.0	30.0	41.0
23	36.24079919376415	40.0	37.0	41.0	29.0	41.0
24	36.167198981947024	40.0	37.0	41.0	29.0	41.0
25	35.9927472043051	40.0	37.0	41.0	28.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.629361331576856
1101	2	0.5992875747262971
1101	3	0.5353032126873387
1101	4	0.5523124304055855
1101	5	0.6046737652867975
1101	6	0.605699965706691
1101	7	0.7065997194765004
1101	8	0.7029162401420237
1101	9	0.957760550701586
1101	10	0.7979060117813859
1101	11	0.6651075541689195
1101	12	0.7787175138803235
1101	13	0.9285624354163602
1101	14	1.0222326193006879
1101	15	1.0624035991439271
1101	16	1.052359268333639
1101	17	0.9749390040361519
1101	18	0.9255001841634822
1101	19	0.9932141760961883
1101	20	0.9926337937391807
1101	21	1.115643911252974
1101	22	1.1621345699517178
1101	23	1.2023338782388535
1101	24	1.1248238953554406
1101	25	1.0640056910306512
1102	1	0.44248084294784107
1102	2	0.5084464458005868
1102	3	0.4349242773308326
1102	4	0.4634322714889123
1102	5	0.4596131952585836
1102	6	0.4693802803598359
1102	7	0.38909398166328657
1102	8	0.5054898671297963
1102	9	0.45884497693780446
1102	10	0.3990225908170544
1102	11	0.4435738305590604
1102	12	0.5085703943619322
1102	13	0.6321854128591227
1102	14	0.774479281547805
1102	15	0.7548266458029005
1102	16	0.8166376250566714
1102	17	0.818457759640971
1102	18	0.6286603951326128
1102	19	0.7333632013986815
1102	20	0.6990300962841616
1102	21	0.6762612917702455
1102	22	0.837952741778615
1102	23	0.8593200969644101
1102	24	0.8773219340346685
1102	25	0.9025453225712567
1103	1	0.5858292075752694
1103	2	0.4818359911096941
1103	3	0.5145841511416229
1103	4	0.5326160468988235
1103	5	0.6313509095350938
1103	6	0.5581328210628769
1103	7	0.5159049303382091
1103	8	0.500192084073035
1103	9	0.5903193097946442
1103	10	0.5607558624599349
1103	11	0.5602081557736511
1103	12	0.50428382837962
1103	13	0.5484192104822014
1103	14	0.5967794608644681
1103	15	0.711652763884878
1103	16	0.6185564744836185
1103	17	0.6745382979132231
1103	18	0.7442213524395953
1103	19	0.7153012485274317
1103	20	0.6994552540206769
1103	21	0.7497086143353755
1103	22	0.7230360814421246
1103	23	0.8090670049604611
1103	24	0.851105561663708
1103	25	0.9302351300648084
1104	1	0.47891484752979707
1104	2	0.3902310479966715
1104	3	0.5058765289419114
1104	4	0.5194715034897186
1104	5	0.48586833391492235
1104	6	0.45300878200959005
1104	7	0.44347279876666335
1104	8	0.4802971281213928
1104	9	0.6246217263157803
1104	10	0.6687279267679926
1104	11	0.6669658766582671
1104	12	0.6577420779158274
1104	13	0.7175095135154734
1104	14	0.8666760879309123
1104	15	0.8229559170334184
1104	16	0.8155234233978632
1104	17	0.8474325038685393
1104	18	0.817928210382803
1104	19	0.8109202093970538
1104	20	0.734126875505595
1104	21	0.8080080166110264
1104	22	0.7755650128956688
1104	23	0.8709363540857566
1104	24	0.8744505898297916
1104	25	0.8451869729924937
1105	1	0.41342812147903985
1105	2	0.5335696252189486
1105	3	0.4384234538755756
1105	4	0.5279098348746345
1105	5	0.5364690194254749
1105	6	0.4507288493098116
1105	7	0.5191063843227468
1105	8	0.5198206995990091
1105	9	0.716542419821188
1105	10	0.7738987336577381
1105	11	0.8473156383652807
1105	12	0.7705416717003075
1105	13	0.72804102692632
1105	14	1.0443509851852397
1105	15	0.8815621709504811
1105	16	0.924882343760288
1105	17	0.9007931194197525
1105	18	0.991574410137801
1105	19	0.9535124399567252
1105	20	0.9690990225391687
1105	21	0.8247001154830471
1105	22	0.9857389009528532
1105	23	0.9395707255691477
1105	24	1.031723522659469
1105	25	1.0458190824747504
1106	1	0.35289279025885634
1106	2	0.4137841419160324
1106	3	0.36338807462926326
1106	4	0.4224623914654231
1106	5	0.3729381386263171
1106	6	0.4839117007810074
1106	7	0.29065954924445947
1106	8	0.336848202560617
1106	9	0.3721464284018552
1106	10	0.44120062588711306
1106	11	0.5186472427580711
1106	12	0.48698133487707906
1106	13	0.4683528765725242
1106	14	0.5546554708254305
1106	15	0.5993203113788681
1106	16	0.7489171787104993
1106	17	0.752153539488269
1106	18	0.7267122451200265
1106	19	0.7205601138913451
1106	20	0.8804043726496289
1106	21	0.6061043994135034
1106	22	0.757399004048942
1106	23	0.8272670723818152
1106	24	0.6906586233542455
1106	25	0.6277068294090782
1107	1	0.259402370543679
1107	2	0.3681238098830484
1107	3	0.19880311585921717
1107	4	0.23574036856047087
1107	5	0.3861705773819537
1107	6	0.23961522112442424
1107	7	0.19765125103100445
1107	8	0.17429889111911478
1107	9	0.2696918492398197
1107	10	0.25900152098704154
1107	11	0.35511238138366963
1107	12	0.38100108556511714
1107	13	0.42111081836491593
1107	14	0.33397855803239196
1107	15	0.35675366194575275
1107	16	0.3496884060000838
1107	17	0.35795153099683574
1107	18	0.3306078620548618
1107	19	0.40664950899387975
1107	20	0.5220552418992384
1107	21	0.6284484749904351
1107	22	0.49966014971418105
1107	23	0.54314397264136
1107	24	0.6020192901358001
1107	25	0.6091447338098988
1108	1	0.3340442959539729
1108	2	0.42131209739530817
1108	3	0.3822220060798287
1108	4	0.44649422170414965
1108	5	0.4238842040551418
1108	6	0.33132939464901057
1108	7	0.1963349016135254
1108	8	0.22167266815587539
1108	9	0.3482935132304803
1108	10	0.43454020044372754
1108	11	0.32456600283504144
1108	12	0.15561710748851
1108	13	0.23609927486658933
1108	14	0.38255581929372795
1108	15	0.3800526149103618
1108	16	0.4105846255300776
1108	17	0.3411658659042516
1108	18	0.3551337357321884
1108	19	0.39976123731153024
1108	20	0.5221477543524387
1108	21	0.6148109058887741
1108	22	0.4867774822306643
1108	23	0.5271900972946995
1108	24	0.5711610418282689
1108	25	0.5676662614577594
1109	1	-0.027684141751116442
1109	2	0.03435137065011773
1109	3	0.053777392767681675
1109	4	-0.01667112664306103
1109	5	0.15257405932784707
1109	6	0.0490697144997867
1109	7	0.14183937468872188
1109	8	-0.022173831101490293
1109	9	0.03851146770528402
1109	10	-0.06892081990099541
1109	11	0.11501883693404125
1109	12	0.05746851158508548
1109	13	0.08669165509525101
1109	14	0.16739917530906467
1109	15	0.0744984026206339
1109	16	0.056323978229464444
1109	17	0.11529802023397906
1109	18	0.03939680091128395
1109	19	0.16517866676129245
1109	20	0.17297156009317405
1109	21	0.09122277421801783
1109	22	0.21426185237331907
1109	23	0.13498602835061746
1109	24	0.20875905225968694
1109	25	0.29555130860639167
1110	1	-0.14445835583550704
1110	2	0.005610154901514619
1110	3	-0.08088473323786616
1110	4	-0.016344010059633263
1110	5	0.09992590644548471
1110	6	-0.04116401370236389
1110	7	-0.09358486889421869
1110	8	-0.10453233766413916
1110	9	-0.013123504620935478
1110	10	-0.015011446409332052
1110	11	-0.06844311754550603
1110	12	0.08357497742272813
1110	13	-0.05626429767583829
1110	14	-0.03398392325372157
1110	15	0.00244687114847153
1110	16	0.07760177087718745
1110	17	0.08074117612024878
1110	18	0.054910214793238765
1110	19	-0.009923997597269363
1110	20	-0.012517155886150988
1110	21	-9.089909042643285E-4
1110	22	-0.18284686944765838
1110	23	-0.13401651230822864
1110	24	-0.13430916916500735
1110	25	-0.06916466351142248
1111	1	-0.005220360957743253
1111	2	-0.020835950635095912
1111	3	-0.13439666936237415
1111	4	0.08622116143108371
1111	5	0.08332476349570328
1111	6	-0.016371923640548403
1111	7	-0.08648032237817205
1111	8	-0.12267709972330465
1111	9	-0.0833454360555308
1111	10	-0.1949420819797325
1111	11	0.03176860415529603
1111	12	-0.08868368975346641
1111	13	0.03393281387659641
1111	14	-0.12735975532773125
1111	15	-0.07530575221270652
1111	16	-0.03601191518411184
1111	17	0.021563899243460583
1111	18	0.06375060905494223
1111	19	-0.009190595169712878
1111	20	0.005378249347643305
1111	21	-0.011674373537680083
1111	22	-0.177170334658328
1111	23	-0.2659050428592664
1111	24	-0.18982503092251335
1111	25	-0.08810150019235152
1112	1	-0.3003620894472405
1112	2	-0.2985919177804064
1112	3	-0.35889763396728114
1112	4	-0.35490831685733326
1112	5	-0.32878594952449447
1112	6	-0.22281012236977915
1112	7	-0.31898347679557304
1112	8	-0.3556480265876871
1112	9	-0.39097678262147895
1112	10	-0.2445559587915298
1112	11	-0.2841643440860153
1112	12	-0.3115857912610238
1112	13	-0.33051591770075817
1112	14	-0.3099066872466807
1112	15	-0.31804571760316946
1112	16	-0.24798688982690464
1112	17	-0.3822632105602608
1112	18	-0.38726733503359156
1112	19	-0.35631149794241423
1112	20	-0.27852794118479096
1112	21	-0.3371939384249387
1112	22	-0.3386457583251783
1112	23	-0.5140655441163489
1112	24	-0.21753928288968893
1112	25	-0.239711518584528
1113	1	-0.2929304353747675
1113	2	-0.25977867960803636
1113	3	-0.2834648217537925
1113	4	-0.14469132990436862
1113	5	-0.21061332258739895
1113	6	-0.12830088088672653
1113	7	-0.2017377738893984
1113	8	-0.15180567507544396
1113	9	-0.11863009097764632
1113	10	-0.1440533936288091
1113	11	-0.16614928862664868
1113	12	-0.1237433933932266
1113	13	-0.26577161060124155
1113	14	-0.13620478743560227
1113	15	-0.05052972260284605
1113	16	-0.028609448567145535
1113	17	0.0863276350041744
1113	18	-0.1456568012588093
1113	19	-0.18505803085587758
1113	20	-0.19066156179790994
1113	21	-0.0650309867130261
1113	22	-0.020533617900767354
1113	23	-0.2676235660279431
1113	24	-0.02972772641432897
1113	25	0.044169853831263595
1114	1	-0.2998823785769602
1114	2	-0.24088974033869093
1114	3	-0.27053234518676916
1114	4	-0.34840750432598355
1114	5	-0.32839555250476593
1114	6	-0.4902165398976166
1114	7	-0.3080326999616787
1114	8	-0.5546746741333308
1114	9	-0.5585022291111557
1114	10	-0.5789994426907867
1114	11	-0.26936951410344534
1114	12	-0.37011781982572245
1114	13	-0.3847717414038314
1114	14	-0.5586167387247869
1114	15	-0.3621800538730042
1114	16	-0.4284240858063981
1114	17	-0.38642987956743724
1114	18	-0.25133745895141146
1114	19	-0.3799184406057279
1114	20	-0.29353304255975843
1114	21	-0.35846467791553493
1114	22	-0.49859694090984163
1114	23	-0.43364918803531793
1114	24	-0.27457357248039216
1114	25	-0.3919481939223175
1115	1	-0.6237843795217621
1115	2	-0.5548946846938279
1115	3	-0.5986492336149389
1115	4	-0.6658477906378621
1115	5	-0.6095811294101878
1115	6	-0.671892379161001
1115	7	-0.8274303633282116
1115	8	-0.7650935421397449
1115	9	-0.7724332251433168
1115	10	-0.6467965338341841
1115	11	-0.7733482009615145
1115	12	-0.5600264610962356
1115	13	-0.8915264115826247
1115	14	-0.8255501535181295
1115	15	-0.8092318861388463
1115	16	-0.979617858790057
1115	17	-0.9504769727974107
1115	18	-0.8519898066854736
1115	19	-1.0033795893587083
1115	20	-0.8807962368268463
1115	21	-0.8524645143278207
1115	22	-0.9567577142457111
1115	23	-0.910669287623513
1115	24	-1.0170080581055387
1115	25	-1.0514570635479146
1116	1	-0.7392075488005005
1116	2	-0.7475872898711344
1116	3	-0.9012042803560334
1116	4	-0.9231288520804952
1116	5	-0.8936345481025327
1116	6	-0.9038146318910876
1116	7	-1.0812500161686742
1116	8	-1.0606160273807106
1116	9	-1.1611790263738229
1116	10	-1.0216494423468205
1116	11	-0.9996577418365362
1116	12	-1.2306607253657518
1116	13	-1.12020464393283
1116	14	-1.3837358965263888
1116	15	-1.3945192192841915
1116	16	-1.4152528293087485
1116	17	-1.2964335256083075
1116	18	-1.582736115155157
1116	19	-1.4519465336416815
1116	20	-1.4776676317398554
1116	21	-1.7611320557982992
1116	22	-1.6429425877010857
1116	23	-1.6700803860798956
1116	24	-1.920179458216964
1116	25	-1.9026579759483369
1201	1	0.5734677759251667
1201	2	0.5366945631899576
1201	3	0.4502070879254667
1201	4	0.4218824913917061
1201	5	0.5210703907900225
1201	6	0.47080661436934435
1201	7	0.4241108283555022
1201	8	0.547335547961552
1201	9	0.6122556374552843
1201	10	0.6366586213549397
1201	11	0.7066943118600975
1201	12	0.7661404251656663
1201	13	0.6610183965441294
1201	14	0.894171128673463
1201	15	0.7870463058638109
1201	16	0.7543470528470664
1201	17	0.9533723125245572
1201	18	0.8622832719581552
1201	19	0.847843265664622
1201	20	0.8302828775776874
1201	21	0.7844973881922712
1201	22	0.8330033396483643
1201	23	0.9034382029685375
1201	24	0.9026202909764933
1201	25	1.0079345302854819
1202	1	0.5992370931430777
1202	2	0.5758947556533869
1202	3	0.5336230427372222
1202	4	0.6541865293964548
1202	5	0.5870467191434798
1202	6	0.47634287463773006
1202	7	0.5607354670630116
1202	8	0.6450437196380321
1202	9	0.6487509506770905
1202	10	0.7320206367090947
1202	11	0.5822870348449101
1202	12	0.5737237553929262
1202	13	0.549382604219403
1202	14	0.6321841244729285
1202	15	0.719881086626053
1202	16	0.6919030609477019
1202	17	0.7812325235340509
1202	18	0.7656575941288324
1202	19	0.6846049103233725
1202	20	0.7378297255936985
1202	21	0.7727727057738747
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2210	19	0.317640860153368
2210	20	0.4033884328326849
2210	21	0.3680503709915328
2210	22	0.309032847634235
2210	23	0.2854676068374076
2210	24	0.2983002516161761
2210	25	0.3898306235498268
2211	1	-0.09081274957012297
2211	2	-0.01663363090746728
2211	3	-0.018155998081297753
2211	4	-0.13356336426581805
2211	5	-0.18374920889515778
2211	6	-0.074943665240788
2211	7	-2.016641301381128E-4
2211	8	-0.10773678695228028
2211	9	-0.08200475206916025
2211	10	-0.1299075843633517
2211	11	-9.59478764201549E-4
2211	12	-0.0410788204982353
2211	13	-0.07198085277718036
2211	14	-0.11777426071458308
2211	15	-0.06767079536464848
2211	16	-0.11408130078221035
2211	17	-0.03547197569968574
2211	18	-0.0874966266244499
2211	19	-0.14547781567641493
2211	20	-0.08049365557998556
2211	21	-0.15560815137192918
2211	22	-0.2236540083644627
2211	23	-0.24374149819465885
2211	24	-0.23547347010561737
2211	25	-0.27067537038850986
2212	1	-0.39051612708826156
2212	2	-0.38929247990934
2212	3	-0.3178874545567396
2212	4	-0.3079088709442601
2212	5	-0.24656527831938035
2212	6	-0.24494925204751894
2212	7	-0.3176167044515168
2212	8	-0.33207443152267757
2212	9	-0.18803469909232007
2212	10	-0.17951585573578654
2212	11	-0.14813397481032098
2212	12	-0.4677824161264681
2212	13	-0.33256607621684253
2212	14	-0.2610765779712949
2212	15	-0.2859147627365388
2212	16	-0.3098159078978142
2212	17	-0.3785220171116208
2212	18	-0.21852517875746713
2212	19	-0.21954738772411986
2212	20	-0.21161622693133353
2212	21	-0.18384743046372165
2212	22	-0.027606185008998807
2212	23	-0.11978123481041791
2212	24	-0.2172083801116571
2212	25	-0.27014313828168923
2213	1	-0.08293549492287866
2213	2	-0.1991997039988469
2213	3	-0.17847682906753448
2213	4	-0.2284148219548996
2213	5	-0.11866387286793412
2213	6	-0.12464200378562396
2213	7	-0.23838596178380556
2213	8	-0.232925213096415
2213	9	-0.2938162152763084
2213	10	-0.33075728029493234
2213	11	-0.11587823099154093
2213	12	-0.2265657682605422
2213	13	-0.24237502805624445
2213	14	-0.28738922473220185
2213	15	-0.272847287254244
2213	16	-0.2956772217244179
2213	17	-0.1434132126086496
2213	18	-0.30059689591755756
2213	19	-0.10626595787007176
2213	20	-0.18326835179895795
2213	21	-0.2527085083218168
2213	22	-0.4131873581753567
2213	23	-0.4026657483699694
2213	24	-0.22506656303904293
2213	25	-0.2925284921193452
2214	1	-0.6470722757518281
2214	2	-0.6662672886793501
2214	3	-0.7143920644274218
2214	4	-0.6995492240860273
2214	5	-0.7071967453879822
2214	6	-0.6967086686459254
2214	7	-0.722133665798502
2214	8	-0.6617812205918838
2214	9	-0.7710085960276771
2214	10	-0.7470957113811494
2214	11	-0.804507684632604
2214	12	-0.7441601817432542
2214	13	-0.8102144417100803
2214	14	-0.809271497729128
2214	15	-0.8787214444422489
2214	16	-0.7601306274834911
2214	17	-0.9971060054965193
2214	18	-0.9465383140663945
2214	19	-1.0929273171504192
2214	20	-0.9330665617860632
2214	21	-0.9838753679843464
2214	22	-0.8800160748193946
2214	23	-0.8449876740313869
2214	24	-0.8696307465239244
2214	25	-0.8394799655429566
2215	1	-0.7854901468201838
2215	2	-0.7820573947446867
2215	3	-0.7983972764347698
2215	4	-0.7665146392179025
2215	5	-0.939015500580787
2215	6	-0.8230251479899309
2215	7	-0.6519722316931151
2215	8	-0.8601692403039252
2215	9	-0.8376914239574447
2215	10	-0.8948477689471872
2215	11	-0.7824762772849141
2215	12	-0.7629066009546719
2215	13	-0.7882531526332386
2215	14	-0.8602168259143497
2215	15	-0.9109293384206012
2215	16	-0.8345433781563045
2215	17	-0.9873824688014068
2215	18	-1.0078532183919506
2215	19	-1.0391629878299184
2215	20	-1.0478822352984878
2215	21	-1.065212738344826
2215	22	-1.1826708441655143
2215	23	-1.1961123147192936
2215	24	-1.1896873555761545
2215	25	-1.3189830560321738
2216	1	-1.0399251146688044
2216	2	-1.074263648069099
2216	3	-1.0408492073606546
2216	4	-0.9061447008874595
2216	5	-1.0770136362748772
2216	6	-1.0540410893854215
2216	7	-0.974867119597775
2216	8	-1.1005356764777758
2216	9	-1.267692398241607
2216	10	-1.2697812806359678
2216	11	-1.059101225279207
2216	12	-1.210176103753767
2216	13	-1.095507488197775
2216	14	-1.3145854371117736
2216	15	-1.3537098927619624
2216	16	-1.5921046132326424
2216	17	-1.5566237910893435
2216	18	-1.5973306860957521
2216	19	-1.63557498890426
2216	20	-1.5352751298704064
2216	21	-1.6531662412913803
2216	22	-1.7231615674003535
2216	23	-1.6848376087161228
2216	24	-1.8707244942352261
2216	25	-1.8285343323444252
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34047.0
3	4.0
4	331.0
5	1918.0
6	2745.0
7	2863.0
8	2425.0
9	2198.0
10	2359.0
11	3220.0
12	3436.0
13	3111.0
14	1665.0
15	1774.0
16	1733.0
17	1947.0
18	2197.0
19	2600.0
20	3284.0
21	4312.0
22	5182.0
23	6348.0
24	7551.0
25	8639.0
26	9997.0
27	11059.0
28	12846.0
29	15380.0
30	19532.0
31	24975.0
32	32349.0
33	42842.0
34	58614.0
35	82156.0
36	122322.0
37	224711.0
38	680082.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.611619393616195	16.98705129487051	11.800833805508338	25.600495506004954
2	15.71200288399211	20.78762101847904	38.267594258359935	25.23278183916891
3	19.201718892565054	26.086025054010054	27.925694920142767	26.78656113328212
4	12.694855266223609	16.196979951404067	36.04974980861191	35.058414973760414
5	14.999213067380152	36.20715117218294	32.768359623353945	16.02527613708297
6	33.21975240142872	35.38467940493116	17.026944550403993	14.36862364323612
7	28.56568147972946	30.79253088145226	21.83484622219386	18.806941416624415
8	27.24735915920374	32.49248620187129	20.717551247467366	19.54260339145761
9	28.483916225530002	14.814840500749348	18.255463725771005	38.44577954794964
10	16.175291732197195	26.74678727057381	30.524327427958024	26.553593569270966
11	36.13354633033603	22.572845672510134	21.3452993003188	19.948308696835042
12	25.488222365202528	24.17365554557172	28.251127015540163	22.08699507368559
13	29.65528392052079	19.804222062860525	24.97430118385299	25.566192832765694
14	24.56654165673695	21.008078958334202	24.656849833389778	29.768529551539068
15	26.93174548085085	25.727126881846324	22.650941463271852	24.690186174030973
16	24.943389022277902	25.821155295930836	25.238253831779346	23.997201850011916
17	24.76360223441266	25.715324901911735	25.173862360793404	24.347210502882202
18	26.22927289475041	24.00184504159008	26.78090409824986	22.98797796540965
19	26.16736262878551	25.57486563935596	24.887103608224432	23.370668123634093
20	25.557155907493616	25.110776175335392	24.79038060735795	24.54168730981304
21	26.496460286145744	25.25130609483046	24.429635338741452	23.822598280282342
22	24.99390331422806	24.976902270475456	23.839643925680356	26.189550489616124
23	24.186222822413523	24.231794623084973	24.53595503582334	27.046027518678166
24	24.144972529042022	26.80957881580176	23.95832607602133	25.087122579134892
25	24.970615714862003	24.539204941715653	24.254175634070023	26.236003709352318
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	345.0
1	345.0
2	500.5
3	656.0
4	656.0
5	656.0
6	1377.0
7	2098.0
8	2098.0
9	2098.0
10	2636.5
11	3175.0
12	3175.0
13	3175.0
14	4340.5
15	5506.0
16	5506.0
17	5506.0
18	8725.0
19	11944.0
20	11944.0
21	11944.0
22	17951.0
23	23958.0
24	23958.0
25	23958.0
26	35375.0
27	46792.0
28	46792.0
29	46792.0
30	58701.0
31	70610.0
32	70610.0
33	70610.0
34	82785.5
35	94961.0
36	94961.0
37	94961.0
38	103106.0
39	111251.0
40	111251.0
41	111251.0
42	125486.5
43	139722.0
44	139722.0
45	139722.0
46	170953.5
47	202185.0
48	202185.0
49	202185.0
50	208944.5
51	215704.0
52	215704.0
53	215704.0
54	182005.5
55	148307.0
56	148307.0
57	148307.0
58	134092.5
59	119878.0
60	119878.0
61	119878.0
62	107031.5
63	94185.0
64	94185.0
65	94185.0
66	78695.0
67	63205.0
68	63205.0
69	63205.0
70	49110.0
71	35015.0
72	35015.0
73	35015.0
74	28813.5
75	22612.0
76	22612.0
77	22612.0
78	19750.5
79	16889.0
80	16889.0
81	16889.0
82	12491.5
83	8094.0
84	8094.0
85	8094.0
86	6059.0
87	4024.0
88	4024.0
89	4024.0
90	2749.0
91	1474.0
92	1474.0
93	1474.0
94	802.0
95	130.0
96	130.0
97	130.0
98	82.0
99	34.0
100	34.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18090402105972328
2	0.021417372608220114
3	0.029804110749303066
4	0.045607220634980045
5	0.0308437890312555
6	0.06265794445899994
7	0.07146055391286388
8	0.07437165310233068
9	0.05787542436201875
10	0.04699345834424995
11	0.6407190692245525
12	0.5692585153116886
13	0.5449993553994652
14	0.5314835377340836
15	0.5447914197430747
16	0.5212253786854862
17	0.5244830373022705
18	0.5237206065621721
19	0.5413258254698999
20	0.5304438594521311
21	0.5372364242275537
22	0.5231661114784641
23	0.5306517951085216
24	0.5142941901391367
25	0.517274601214067
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1442754.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.491246572521355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.59688014814749	44.58981308119342
2	8.473290355306728	8.726005659313318
3	2.0045122242478723	3.0964449958914124
4	0.8211740070590177	1.6913309310568507
5	0.46372514622518707	1.1938892923079814
6	0.28574005696100746	0.882786703717532
7	0.19776029075517124	0.7128046725469626
8	0.141092913555764	0.5812040001228228
9	0.11347998177376718	0.5258903150302446
>10	0.6674029357254577	6.666757108918635
>50	0.09496052825149316	3.5031006254907857
>100	0.11277033854196361	12.662891983577119
>500	0.018682229532217244	6.642132907017715
>1k	0.008122708492268366	6.750528947007352
>5k	2.707569497422789E-4	1.0348859226277483
>10k+	1.3537847487113944E-4	0.7395328541801012
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAAC	10609	0.7353297928822239	No Hit
CCCATGTACTCTGCGTTGATACCAC	9292	0.6440460397268003	No Hit
GTACGGAAGCAGTGGTATCAACGCA	5554	0.38495821186425405	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3677	0.254859802849273	No Hit
CTTCCGTACTCTGCGTTGATACCAC	3589	0.2487603569284854	No Hit
GAGTACGGAAGCAGTGGTATCAACG	2794	0.19365740798500644	No Hit
GCGTTGATACCACTGCTTCCCATGT	2787	0.19317222478676196	No Hit
GTATCAACGCAGAGTACATGGGAAG	2734	0.18949869485719673	No Hit
CATGTACTCTGCGTTGATACCACTG	2654	0.18395374402011708	No Hit
TCCGTACTCTGCGTTGATACCACTG	2628	0.1821516349980662	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2254	0.1562289898347189	No Hit
GCTTCCGTACTCTGCGTTGATACCA	2174	0.15068403899763924	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2108	0.14610945455704852	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2096	0.1452777119314866	No Hit
TATCAACGCAGAGTACATGGGAAGC	2064	0.14305973159665472	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1987	0.13772271641596556	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1986	0.1376534045305021	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1948	0.13501955288288925	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1934	0.13404918648640032	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1915	0.13273226066259391	No Hit
CTTTAATATACGCTATTGGAGCTGG	1737	0.1203947450500917	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1725	0.11956300242452975	No Hit
GAACTACGACGGTATCTGATCGTCT	1677	0.11623603192228198	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1654	0.11464185855662157	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1614	0.11186938313808176	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1572	0.10895828394861494	No Hit
GGTATCAACGCAGAGTACATGGGAA	1510	0.10466094704987823	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	1502	0.10410645196617026	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	1500	0.10396782819524326	No Hit
GCGTTGATACCACTGCTTCCGTACT	1500	0.10396782819524326	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	1471	0.1019577835168019	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	6.931188546349551E-5	6.931188546349551E-5
10	0.0	0.0	0.0	1.3862377092699102E-4	6.931188546349551E-5
11	0.0	0.0	0.0	2.0793565639048653E-4	1.3862377092699102E-4
12	0.0	0.0	0.0	2.7724754185398204E-4	1.3862377092699102E-4
13	0.0	0.0	0.0	5.544950837079641E-4	1.3862377092699102E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGCTA	40	0.0052301832	14.269904	8
CCGCTAC	40	0.0052301832	14.269904	9
CGAACGA	120	3.6379788E-12	14.253997	16
GTCATAC	50	0.0015243975	13.270909	1
GTACTAG	50	0.0015243975	13.270909	1
CCAACGT	75	1.4741399E-5	12.67022	19
ATCGCCA	270	0.0	12.318269	16
CGGAATT	85	3.927318E-6	12.298423	15
CGCGGTA	55	0.0030618114	12.094301	17
ATACCGT	270	0.0	11.979672	6
GGTTCTA	305	0.0	11.841036	13
AAGCTTA	185	0.0	11.814947	15
AAGCTCG	105	2.6702946E-7	11.778334	7
GGACTAT	65	7.929367E-4	11.708638	6
TACCGTC	255	0.0	11.565151	7
ACGGTAT	380	0.0	11.516063	9
GCATCGC	290	0.0	11.469532	14
TAGAGTG	250	0.0	11.415924	5
ACCGTCG	250	0.0	11.415924	8
CCGGTAG	75	2.0468935E-4	11.415923	9
>>END_MODULE