FastQCFastQC Report
Thu 2 Feb 2017
SRR4050282_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050282_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1442754
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCAC100190.6944357804587615No Hit
GTACATGGGAAGCAGTGGTATCAAC90150.624846647453412No Hit
GTACGGAAGCAGTGGTATCAACGCA48740.3378261297490771No Hit
GTATCAACGCAGAGTACTTTTTTTT34940.24217572780945332No Hit
CTTCCGTACTCTGCGTTGATACCAC34610.23988843558915796No Hit
GAGTACATGGGAAGCAGTGGTATCA33550.23254137573002742No Hit
CATGTACTCTGCGTTGATACCACTG31020.21500546870776308No Hit
GAGTACGGAAGCAGTGGTATCAACG29610.20523249285741021No Hit
TCCGTACTCTGCGTTGATACCACTG27530.19081562068100313No Hit
GCGTTGATACCACTGCTTCCCATGT27360.18963731862812372No Hit
GTATCAACGCAGAGTACATGGGAAG24760.1716162284076149No Hit
ACTCTGCGTTGATACCACTGCTTCC23600.1635760496938494No Hit
TATCAACGCAGAGTACTTTTTTTTT22610.15671417303296337No Hit
TATCAACGCAGAGTACATGGGAAGC21310.14770362792270894No Hit
GCTTCCGTACTCTGCGTTGATACCA20550.14243592462748328No Hit
GAATAGGACCGCGGTTCTATTTTGT20540.14236661274201978No Hit
GATTAAGAGGGACGGCCGGGGGCAT20420.14153487011645782No Hit
CTATTGGAGCTGGAATTACCGCGGC20280.1405645037199689No Hit
ACGCAGAGTACATGGGAAGCAGTGG18720.1297518495876636No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG18350.12718730982551424No Hit
CTTTAATATACGCTATTGGAGCTGG18290.1267714385127333No Hit
GTATCTGATCGTCTTCGAACCTCCG17540.12157304710297112No Hit
CTCTTAATCATGGCCTCAGTTCCGA17180.11907781922628527No Hit
GAACTACGACGGTATCTGATCGTCT16860.11685983889145343No Hit
GTCCTATTCCATTATTCCTAGCTGC16540.11464185855662157No Hit
GAATAACGCCGCCGCATCGCCAGTC16480.11422598724384062No Hit
ATACAGGACTCTTTCGAGGCCCTGT16130.11180007125261826No Hit
ATATTAAAGTTGCTGCAGTTAAAAA15970.11069108108520233No Hit
GTCTTGCGCCGGTCCAAGAATTTCA15500.10743342246841804No Hit
GCGCAAGACGGACCAGAGCGAAAGC14910.1033440212260718No Hit
CTCTAGATAACCTCGGGCCGATCGC14730.1020964072877289No Hit
GCTTCCCATGTACTCTGCGTTGATA14680.10174984786041141No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGCTC456.765937E-414.7766175
GTGCTAC400.00515885114.2994731
CGTCTTA602.5876148E-514.2385115
CGGTTCT4250.012.95649712
GGTTCTA4400.012.72880613
ATAGGGT905.296515E-712.6876753
TTCTCGT751.4880656E-512.65864711
GTTATTC1850.012.3447663
TCTCGTC701.0972631E-412.206552512
GTGTTAC550.002989631412.1328861
CGCCCGA802.8897804E-511.86542516
CGCAAGA3050.011.8556982
AAGACGG3100.011.6442475
CCAACGT907.4371474E-611.61422719
GAACTAT907.4774653E-611.6085886
CGTCGTA2300.011.55789510
GCGTTAT1751.8189894E-1211.4395781
CACTAAG752.0079187E-411.4395781
CGAGGTT1501.70985E-1011.4189094
AGACTGT1251.8366336E-811.3975246
Produced by FastQC (version 0.11.5)