##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050282_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1442754 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.297895552533557 31.0 31.0 34.0 28.0 34.0 2 31.114964851942883 31.0 31.0 34.0 27.0 34.0 3 31.449436286435525 33.0 31.0 34.0 28.0 34.0 4 35.101710340085695 37.0 35.0 37.0 32.0 37.0 5 35.03013888715609 37.0 35.0 37.0 32.0 37.0 6 35.06099307297017 37.0 35.0 37.0 32.0 37.0 7 35.00020516318097 37.0 35.0 37.0 32.0 37.0 8 34.99030534658022 37.0 35.0 37.0 32.0 37.0 9 36.65667189278283 39.0 37.0 39.0 32.0 39.0 10 36.54699761705738 39.0 37.0 39.0 32.0 39.0 11 36.56778633086444 39.0 37.0 39.0 32.0 39.0 12 36.462811400973415 39.0 37.0 39.0 32.0 39.0 13 36.476576048307614 39.0 37.0 39.0 32.0 39.0 14 37.730493902633434 40.0 38.0 41.0 32.0 41.0 15 37.72107095180468 40.0 38.0 41.0 32.0 41.0 16 37.6967743634743 40.0 38.0 41.0 32.0 41.0 17 37.64134980738227 40.0 38.0 41.0 32.0 41.0 18 37.62085636220728 40.0 38.0 41.0 32.0 41.0 19 37.65692626740248 40.0 38.0 41.0 32.0 41.0 20 37.586038229663544 40.0 38.0 41.0 32.0 41.0 21 37.52454403176148 40.0 38.0 41.0 32.0 41.0 22 37.4641470410063 40.0 38.0 41.0 32.0 41.0 23 37.404751606996065 40.0 38.0 41.0 32.0 41.0 24 37.345355479866974 40.0 38.0 41.0 32.0 41.0 25 37.1719953644211 40.0 37.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.38727436474415455 1101 2 0.660295731909109 1101 3 0.44639083568648985 1101 4 0.4578868545571737 1101 5 0.5198490515578484 1101 6 0.44236924204660255 1101 7 0.4120860223059992 1101 8 0.37543994450894047 1101 9 0.4997874907710269 1101 10 0.584487848424601 1101 11 0.5414001738712955 1101 12 0.5739339817906028 1101 13 0.5117355708114601 1101 14 0.6019386075899789 1101 15 0.6047498201058374 1101 16 0.6244951071581397 1101 17 0.6558505480690044 1101 18 0.6176929817128922 1101 19 0.708376572757139 1101 20 0.7264456911168224 1101 21 0.4183529482802726 1101 22 0.6571460216690923 1101 23 0.6545825271081398 1101 24 0.7147993415217186 1101 25 0.7953053361843558 1102 1 0.37634545282996257 1102 2 0.7376022860526703 1102 3 0.5941833413950768 1102 4 0.42955614606146497 1102 5 0.4758492900731568 1102 6 0.4790631859791361 1102 7 0.4943036534981857 1102 8 0.6114412586815448 1102 9 0.6182657630783481 1102 10 0.6794078230297345 1102 11 0.6439783607106264 1102 12 0.6368716252359334 1102 13 0.7077757489496577 1102 14 0.6989135552978354 1102 15 0.6322356699510934 1102 16 0.6646451847340771 1102 17 0.7526431017144901 1102 18 0.7881537956346989 1102 19 0.7500896271444759 1102 20 0.749515500105808 1102 21 0.7677509894148073 1102 22 0.8397486680198654 1102 23 0.8569361120283645 1102 24 0.7443974549123666 1102 25 0.7020998657655184 1103 1 0.2924148960275552 1103 2 0.731496081788201 1103 3 0.4922290429743974 1103 4 0.36339313133171913 1103 5 0.316203826048131 1103 6 0.41729101251846146 1103 7 0.3936814546017118 1103 8 0.41769616456907244 1103 9 0.40296768077683254 1103 10 0.40040375560298713 1103 11 0.3267262101767088 1103 12 0.40425716987289206 1103 13 0.5335777824328432 1103 14 0.7715158567201712 1103 15 0.6952098865628642 1103 16 0.5834359136712735 1103 17 0.6175166017501681 1103 18 0.6162775080190599 1103 19 0.5497496390682315 1103 20 0.6764588070365463 1103 21 0.7562208589210755 1103 22 0.7796043860116129 1103 23 0.8010673469654179 1103 24 0.7458706598466804 1103 25 0.5945872111653188 1104 1 0.2893228880115508 1104 2 0.5539815776257875 1104 3 0.39207779594396897 1104 4 0.379754013698161 1104 5 0.4527020428572328 1104 6 0.33796230156294627 1104 7 0.4084590113962179 1104 8 0.31367550691632573 1104 9 0.3513063921289614 1104 10 0.3096129796219387 1104 11 0.3764752978594288 1104 12 0.37790970544423885 1104 13 0.32107236245050075 1104 14 0.531104379236325 1104 15 0.48147898239171383 1104 16 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2214 17 -0.8040920591580516 2214 18 -0.7861698638805947 2214 19 -0.8381288353767928 2214 20 -0.7311903733057221 2214 21 -0.8024435037290942 2214 22 -0.877249377497094 2214 23 -1.0112444316732834 2214 24 -0.866123271543195 2214 25 -0.8518238385293131 2215 1 -0.845042992632667 2215 2 -2.110483534191129 2215 3 -1.255320088896287 2215 4 -0.8155147141313108 2215 5 -0.7633931212449738 2215 6 -0.8937824732501554 2215 7 -0.9095963989005824 2215 8 -0.9058389609870829 2215 9 -0.9335139671289312 2215 10 -0.981536817063855 2215 11 -1.0028470457860053 2215 12 -1.021724006575404 2215 13 -0.9654492935989509 2215 14 -1.1561406916425199 2215 15 -1.06126850773353 2215 16 -1.1910652653672713 2215 17 -1.094907763677547 2215 18 -1.1011259853465774 2215 19 -1.1120597783935438 2215 20 -1.1829331470717648 2215 21 -1.1768230979733403 2215 22 -1.0834032599704173 2215 23 -1.2299130160805305 2215 24 -1.1274794364190868 2215 25 -1.1468862790256793 2216 1 -0.8838096278764773 2216 2 -1.9848686818873418 2216 3 -1.1825476448122991 2216 4 -0.8712365270371549 2216 5 -0.8575794096436198 2216 6 -0.8331062937080205 2216 7 -0.8370273695509312 2216 8 -0.916633107909135 2216 9 -0.9348634823567323 2216 10 -1.103627130572697 2216 11 -1.1811818624889199 2216 12 -1.0697548992830193 2216 13 -1.0642087928695574 2216 14 -1.1922102713310636 2216 15 -1.0847059665897731 2216 16 -1.1656851628831078 2216 17 -1.0805067904466625 2216 18 -1.0432679885801477 2216 19 -1.0515231584749642 2216 20 -1.1371773395481881 2216 21 -1.263321757674312 2216 22 -1.084513153957765 2216 23 -1.3178704688277492 2216 24 -1.3238134947602802 2216 25 -1.3760662214112571 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11641.0 3 0.0 4 189.0 5 1714.0 6 2488.0 7 1821.0 8 1510.0 9 1238.0 10 1194.0 11 1188.0 12 1068.0 13 1090.0 14 1052.0 15 1104.0 16 1156.0 17 1327.0 18 1476.0 19 1757.0 20 2315.0 21 3049.0 22 3915.0 23 4749.0 24 5831.0 25 7047.0 26 8274.0 27 9552.0 28 11021.0 29 13970.0 30 18028.0 31 24051.0 32 31904.0 33 43252.0 34 60374.0 35 85748.0 36 128461.0 37 230988.0 38 717212.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.55938935156446 17.82833185434538 12.421068565326134 26.19121022876402 2 15.440091333759872 21.385727782972797 37.429719933371274 25.74446094989606 3 19.323529860253377 25.590225360664398 28.222482834911567 26.863761944170662 4 13.152338947232495 16.676883643788273 35.86145084777769 34.309326561201544 5 14.906849096576666 36.36356071192047 32.872126850275485 15.85746334122738 6 33.304480990745056 35.81817198215594 16.60854584193355 14.268801185165458 7 28.314698368758716 30.872232839513707 21.866358040957167 18.94671075077041 8 27.070983558593227 32.862291258474116 20.613533342485166 19.4531918404475 9 28.001220142049494 14.779317205736053 18.408546540446668 38.81091611176778 10 16.061036486159548 26.963558206422388 30.637199342551753 26.338205964866308 11 36.26445467402457 22.237042788608193 21.464601647208898 20.033900890158343 12 25.369254073840413 24.23152516404552 28.19010908637131 22.209111675742754 13 29.92921793127256 19.692194008444496 24.98674265924579 25.391845401037155 14 24.427430116285116 20.75004765783408 24.882172504029253 29.94034972185155 15 26.889793496666403 25.888848764535737 22.633369768710445 24.58798797008741 16 25.19741798827379 25.71933757107082 25.02885113231597 24.054393308339417 17 24.66366407578839 25.777921946499543 25.29883819417586 24.259575783536206 18 26.16752405468985 23.82776273709863 26.94042088949329 23.06429231871823 19 26.32326786132633 25.59729517298167 24.869173816187654 23.21026314950435 20 25.466521296438128 24.85657628159498 25.162657779952628 24.514244642014262 21 26.41560308562691 25.191292094138102 24.535780060706085 23.857324759528908 22 24.972827443173312 24.904688097865566 24.04355372679935 26.07893073216177 23 24.17594756672683 23.958238029094478 24.90719099939837 26.958623404780326 24 24.24187183889443 26.59691931482723 24.231336264348656 24.929872581929686 25 25.03858431644691 24.326995142262316 24.393962526023593 26.240458015267176 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 348.0 1 348.0 2 410.5 3 473.0 4 473.0 5 473.0 6 1054.5 7 1636.0 8 1636.0 9 1636.0 10 1935.5 11 2235.0 12 2235.0 13 2235.0 14 3087.0 15 3939.0 16 3939.0 17 3939.0 18 7234.5 19 10530.0 20 10530.0 21 10530.0 22 17390.0 23 24250.0 24 24250.0 25 24250.0 26 36213.0 27 48176.0 28 48176.0 29 48176.0 30 59580.0 31 70984.0 32 70984.0 33 70984.0 34 83424.0 35 95864.0 36 95864.0 37 95864.0 38 104116.5 39 112369.0 40 112369.0 41 112369.0 42 125963.5 43 139558.0 44 139558.0 45 139558.0 46 170443.0 47 201328.0 48 201328.0 49 201328.0 50 209227.0 51 217126.0 52 217126.0 53 217126.0 54 182814.0 55 148502.0 56 148502.0 57 148502.0 58 134179.0 59 119856.0 60 119856.0 61 119856.0 62 107024.0 63 94192.0 64 94192.0 65 94192.0 66 78913.0 67 63634.0 68 63634.0 69 63634.0 70 49104.0 71 34574.0 72 34574.0 73 34574.0 74 28550.5 75 22527.0 76 22527.0 77 22527.0 78 19717.0 79 16907.0 80 16907.0 81 16907.0 82 12512.0 83 8117.0 84 8117.0 85 8117.0 86 6076.5 87 4036.0 88 4036.0 89 4036.0 90 2737.0 91 1438.0 92 1438.0 93 1438.0 94 785.5 95 133.0 96 133.0 97 133.0 98 77.5 99 22.0 100 22.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1788246644958184 2 0.009218480766644903 3 0.0 4 0.162259123870043 5 0.05122148335752318 6 0.06446005348105083 7 0.045884468176834024 8 0.029110991894668113 9 0.020724253753585158 10 0.01434756029094357 11 0.02176393203553759 12 0.022110491462855066 13 0.010881966017768795 14 0.012406827497965695 15 0.0011783020528794237 16 2.0793565639048655E-4 17 0.0 18 0.0 19 0.0 20 6.931188546349551E-5 21 0.004505272555127208 22 0.008456050026546452 23 4.158713127809731E-4 24 0.0015941733656603967 25 0.12157304710297112 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1442754.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.80523440300728 #Duplication Level Percentage of deduplicated Percentage of total 1 86.42610114964371 43.908983274456666 2 8.63701977573433 8.776116284991842 3 1.9770670621864235 3.0133606657453864 4 0.8223888083166762 1.6712662470775426 5 0.4639456650529223 1.1785434131636412 6 0.2945577359883635 0.8979044893264765 7 0.1994178524723887 0.7092029517302825 8 0.15148764486421928 0.6157092245188946 9 0.11008719529570596 0.5033705185591184 >10 0.6750490729583156 6.615828300322002 >50 0.09924969839121825 3.6242349190921375 >100 0.11487516823669534 12.744500978573905 >500 0.019948372127119304 7.043348490587337 >1k 0.008529648771595838 7.367243269840902 >5k 1.3757498018702967E-4 0.6301060498426599 >10k+ 1.3757498018702967E-4 0.7002809221712268 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCAC 10019 0.6944357804587615 No Hit GTACATGGGAAGCAGTGGTATCAAC 9015 0.624846647453412 No Hit GTACGGAAGCAGTGGTATCAACGCA 4874 0.3378261297490771 No Hit GTATCAACGCAGAGTACTTTTTTTT 3494 0.24217572780945332 No Hit CTTCCGTACTCTGCGTTGATACCAC 3461 0.23988843558915796 No Hit GAGTACATGGGAAGCAGTGGTATCA 3355 0.23254137573002742 No Hit CATGTACTCTGCGTTGATACCACTG 3102 0.21500546870776308 No Hit GAGTACGGAAGCAGTGGTATCAACG 2961 0.20523249285741021 No Hit TCCGTACTCTGCGTTGATACCACTG 2753 0.19081562068100313 No Hit GCGTTGATACCACTGCTTCCCATGT 2736 0.18963731862812372 No Hit GTATCAACGCAGAGTACATGGGAAG 2476 0.1716162284076149 No Hit ACTCTGCGTTGATACCACTGCTTCC 2360 0.1635760496938494 No Hit TATCAACGCAGAGTACTTTTTTTTT 2261 0.15671417303296337 No Hit TATCAACGCAGAGTACATGGGAAGC 2131 0.14770362792270894 No Hit GCTTCCGTACTCTGCGTTGATACCA 2055 0.14243592462748328 No Hit GAATAGGACCGCGGTTCTATTTTGT 2054 0.14236661274201978 No Hit GATTAAGAGGGACGGCCGGGGGCAT 2042 0.14153487011645782 No Hit CTATTGGAGCTGGAATTACCGCGGC 2028 0.1405645037199689 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1872 0.1297518495876636 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 1835 0.12718730982551424 No Hit CTTTAATATACGCTATTGGAGCTGG 1829 0.1267714385127333 No Hit GTATCTGATCGTCTTCGAACCTCCG 1754 0.12157304710297112 No Hit CTCTTAATCATGGCCTCAGTTCCGA 1718 0.11907781922628527 No Hit GAACTACGACGGTATCTGATCGTCT 1686 0.11685983889145343 No Hit GTCCTATTCCATTATTCCTAGCTGC 1654 0.11464185855662157 No Hit GAATAACGCCGCCGCATCGCCAGTC 1648 0.11422598724384062 No Hit ATACAGGACTCTTTCGAGGCCCTGT 1613 0.11180007125261826 No Hit ATATTAAAGTTGCTGCAGTTAAAAA 1597 0.11069108108520233 No Hit GTCTTGCGCCGGTCCAAGAATTTCA 1550 0.10743342246841804 No Hit GCGCAAGACGGACCAGAGCGAAAGC 1491 0.1033440212260718 No Hit CTCTAGATAACCTCGGGCCGATCGC 1473 0.1020964072877289 No Hit GCTTCCCATGTACTCTGCGTTGATA 1468 0.10174984786041141 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.3862377092699102E-4 2 0.0 0.0 0.0 0.0 1.3862377092699102E-4 3 0.0 0.0 0.0 0.0 1.3862377092699102E-4 4 0.0 0.0 0.0 6.931188546349551E-5 1.3862377092699102E-4 5 0.0 0.0 0.0 6.931188546349551E-5 1.3862377092699102E-4 6 0.0 0.0 0.0 6.931188546349551E-5 1.3862377092699102E-4 7 0.0 0.0 0.0 6.931188546349551E-5 1.3862377092699102E-4 8 0.0 0.0 0.0 6.931188546349551E-5 1.3862377092699102E-4 9 0.0 0.0 0.0 2.7724754185398204E-4 1.3862377092699102E-4 10 0.0 0.0 0.0 4.1587131278097305E-4 1.3862377092699102E-4 11 0.0 0.0 0.0 4.8518319824446856E-4 1.3862377092699102E-4 12 0.0 0.0 0.0 6.238069691714596E-4 2.0793565639048653E-4 13 0.0 0.0 0.0 8.317426255619461E-4 2.0793565639048653E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGCTC 45 6.765937E-4 14.776617 5 GTGCTAC 40 0.005158851 14.299473 1 CGTCTTA 60 2.5876148E-5 14.23851 15 CGGTTCT 425 0.0 12.956497 12 GGTTCTA 440 0.0 12.728806 13 ATAGGGT 90 5.296515E-7 12.687675 3 TTCTCGT 75 1.4880656E-5 12.658647 11 GTTATTC 185 0.0 12.344766 3 TCTCGTC 70 1.0972631E-4 12.2065525 12 GTGTTAC 55 0.0029896314 12.132886 1 CGCCCGA 80 2.8897804E-5 11.865425 16 CGCAAGA 305 0.0 11.855698 2 AAGACGG 310 0.0 11.644247 5 CCAACGT 90 7.4371474E-6 11.614227 19 GAACTAT 90 7.4774653E-6 11.608588 6 CGTCGTA 230 0.0 11.557895 10 GCGTTAT 175 1.8189894E-12 11.439578 1 CACTAAG 75 2.0079187E-4 11.439578 1 CGAGGTT 150 1.70985E-10 11.418909 4 AGACTGT 125 1.8366336E-8 11.397524 6 >>END_MODULE