FastQCFastQC Report
Thu 2 Feb 2017
SRR4050281_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050281_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9748836
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA154280.15825479062320874No Hit
GTATCTGATCGTCTTCGAACCTCCG112960.1158702433808508No Hit
CTATTGGAGCTGGAATTACCGCGGC108290.11107992790113609No Hit
GAATAGGACCGCGGTTCTATTTTGT106510.10925406889602No Hit
GATTAAGAGGGACGGCCGGGGGCAT103380.10604342918477652No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG99130.10168393436919033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47100.015.6936261
GTATCAA172300.014.0016021
TAGATCG3050.011.537095
ACCGTCC5850.010.7301298
CTCGACG3700.010.5371413
CGATACT909.895945E-510.5159474
GGTAGCG5100.010.44328111
CGAACGA8600.010.28275316
ACGCTTA3450.010.19729817
GTATTAG17450.010.1966051
CGTCGTA15700.010.11663110
CGCTTAC3600.010.03663318
CGCCAGT12950.09.91222118
GGGTAGG21400.09.9066631
AGGCCCG20550.09.85795610
CCGGTTT5800.09.83672112
CGCGTAT2350.09.7126517
ACGCATC7950.09.69029410
CACTCTA17000.09.6786349
ACTCTAA17600.09.67296110
Produced by FastQC (version 0.11.5)