##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050281_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9748836 Sequences flagged as poor quality 0 Sequence length 25 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.513292150980895 31.0 31.0 34.0 27.0 34.0 2 30.65388001193168 31.0 31.0 34.0 27.0 34.0 3 30.7553930541041 31.0 31.0 34.0 27.0 34.0 4 34.29830504893097 37.0 35.0 37.0 31.0 37.0 5 34.307185186005796 37.0 35.0 37.0 31.0 37.0 6 34.34641366415437 37.0 35.0 37.0 31.0 37.0 7 34.27019543666547 37.0 35.0 37.0 30.0 37.0 8 34.24906388824266 37.0 35.0 37.0 30.0 37.0 9 35.813722068973156 39.0 37.0 39.0 31.0 39.0 10 35.698671410617635 39.0 37.0 39.0 30.0 39.0 11 35.600759106010194 39.0 37.0 39.0 30.0 39.0 12 35.5306491975042 39.0 37.0 39.0 30.0 39.0 13 35.53973889805921 39.0 37.0 39.0 30.0 39.0 14 36.70430439080112 40.0 37.0 41.0 30.0 41.0 15 36.660636100556005 40.0 37.0 41.0 30.0 41.0 16 36.67073002356384 40.0 37.0 41.0 30.0 41.0 17 36.64101980995475 40.0 37.0 41.0 30.0 41.0 18 36.604455855037465 40.0 37.0 41.0 30.0 41.0 19 36.62496138000475 40.0 37.0 41.0 30.0 41.0 20 36.57918955657886 40.0 37.0 41.0 30.0 41.0 21 36.53012677616076 40.0 37.0 41.0 30.0 41.0 22 36.45089906118023 40.0 37.0 41.0 30.0 41.0 23 36.37480515622583 40.0 37.0 41.0 30.0 41.0 24 36.31489513209577 40.0 37.0 41.0 30.0 41.0 25 36.08400808055444 40.0 37.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.586775343555562 1101 2 0.6171116829587433 1101 3 0.5872242450374792 1101 4 0.6005922276557811 1101 5 0.6186772407007695 1101 6 0.6674018235463279 1101 7 0.6265386960148192 1101 8 0.6353682644316123 1101 9 0.6780423859441527 1101 10 0.6574765924764492 1101 11 0.5495106826494336 1101 12 0.640989332246015 1101 13 0.5406058384504604 1101 14 0.6718939083501496 1101 15 0.6679168751075437 1101 16 0.7460115761763149 1101 17 0.704997624311865 1101 18 0.7506705344454829 1101 19 0.7879691458901732 1101 20 0.7302527335892677 1101 21 0.7190619139151124 1101 22 0.7908534562586453 1101 23 0.8059093839164362 1101 24 0.8476502036945348 1101 25 0.8803299517861944 1102 1 0.576730764895796 1102 2 0.6016153568711822 1102 3 0.6034871060504052 1102 4 0.5843720202096137 1102 5 0.5676584258158641 1102 6 0.5674493710242245 1102 7 0.58058340919915 1102 8 0.5931283143448667 1102 9 0.6819516468012168 1102 10 0.7338584613474666 1102 11 0.638697806328544 1102 12 0.6294612026942161 1102 13 0.6522751260268791 1102 14 0.8214622236386333 1102 15 0.7825181721099597 1102 16 0.7623670503986233 1102 17 0.7673362644076107 1102 18 0.837598039617717 1102 19 0.8524984722467863 1102 20 0.8518006475306521 1102 21 0.8614147950603339 1102 22 0.8847781874414409 1102 23 0.8514492681325265 1102 24 0.8310345172017293 1102 25 0.8911987949676501 1103 1 0.498848234123372 1103 2 0.5153359154797172 1103 3 0.4956683194852758 1103 4 0.4912766609769932 1103 5 0.4888350799449057 1103 6 0.43372780287391777 1103 7 0.45003124364620817 1103 8 0.4732616974761541 1103 9 0.482373607791466 1103 10 0.4954043788440927 1103 11 0.5043983270208869 1103 12 0.3958760279868301 1103 13 0.4314967693205176 1103 14 0.5721813710634365 1103 15 0.609890686270731 1103 16 0.6187199102004044 1103 17 0.5412234798201325 1103 18 0.5866867702127934 1103 19 0.5857646065728446 1103 20 0.5773655347979201 1103 21 0.5642300499461683 1103 22 0.6436911604767204 1103 23 0.6264922142913321 1103 24 0.6616257710217042 1103 25 0.6591771463630494 1104 1 0.44033002927971765 1104 2 0.4654306731663205 1104 3 0.4752275273629323 1104 4 0.46634586685456725 1104 5 0.47325526639565396 1104 6 0.4594715525044393 1104 7 0.4831531500668049 1104 8 0.4573210434974655 1104 9 0.5416556489029745 1104 10 0.5476152729998063 1104 11 0.4368889365614024 1104 12 0.4174721295874235 1104 13 0.4696082069983234 1104 14 0.5041941729364936 1104 15 0.3945678120705267 1104 16 0.533578359224606 1104 17 0.536533725345663 1104 18 0.5698800031815594 1104 19 0.5806361262416857 1104 20 0.6002539610516919 1104 21 0.5932725646520467 1104 22 0.6075284423928693 1104 23 0.6111408969684504 1104 24 0.6223305179249152 1104 25 0.7063037841057351 1105 1 0.34885979264445055 1105 2 0.4177389552901829 1105 3 0.4034984923106819 1105 4 0.48766443064709364 1105 5 0.48185132033850664 1105 6 0.36774090438145635 1105 7 0.433381543205293 1105 8 0.4459627716486452 1105 9 0.4598030482131108 1105 10 0.5011322975251105 1105 11 0.4930172574431566 1105 12 0.4163656956448776 1105 13 0.49213986627358963 1105 14 0.6214214811191852 1105 15 0.5983416241986106 1105 16 0.47066623622465187 1105 17 0.5310567756845188 1105 18 0.6104886390761379 1105 19 0.5981336790145093 1105 20 0.5291614702274146 1105 21 0.5640829227758459 1105 22 0.5251496049970257 1105 23 0.5286079339784848 1105 24 0.5435023143774842 1105 25 0.5845861510329229 1106 1 0.2938536548630317 1106 2 0.31297623574545597 1106 3 0.33622123935769466 1106 4 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9 -0.5115900597902225 2214 10 -0.5540979880815016 2214 11 -0.43324185184483355 2214 12 -0.4262564697455673 2214 13 -0.5159607898575302 2214 14 -0.5618290460901108 2214 15 -0.4347139725116307 2214 16 -0.4835613548932045 2214 17 -0.5303837314130178 2214 18 -0.6183640712152325 2214 19 -0.6000262469910922 2214 20 -0.6553697338327638 2214 21 -0.6929992322983196 2214 22 -0.5816086898246411 2214 23 -0.5643712386879827 2214 24 -0.6408938838337122 2214 25 -0.5805544049508242 2215 1 -0.57423513599716 2215 2 -0.5526796039989073 2215 3 -0.7157086065959533 2215 4 -0.6776687133734285 2215 5 -0.6681926503924416 2215 6 -0.6230998750533416 2215 7 -0.5924379122453232 2215 8 -0.7381138773852314 2215 9 -0.7970210514689811 2215 10 -0.7305305323211044 2215 11 -0.6222528025342129 2215 12 -0.6940050459799494 2215 13 -0.6802995667750054 2215 14 -0.8004729292583974 2215 15 -0.7671158069155979 2215 16 -0.8285984574627818 2215 17 -0.8135401235496929 2215 18 -0.8250952918263081 2215 19 -0.9071698504962455 2215 20 -0.8660400164444368 2215 21 -0.8394126087542446 2215 22 -0.8925788718964967 2215 23 -0.8132801942882253 2215 24 -0.9108792051328578 2215 25 -0.8691261800670844 2216 1 -0.03321431305410982 2216 2 -0.12837638058981682 2216 3 -0.01731629875124341 2216 4 0.026814863698852776 2216 5 0.033281941637433476 2216 6 0.12458447068713241 2216 7 0.011135322970027062 2216 8 0.0714662172330165 2216 9 0.17949567064182048 2216 10 0.1439809932118692 2216 11 0.35827203928288753 2216 12 0.16742056212498824 2216 13 0.23886213334320416 2216 14 0.22113714039752352 2216 15 0.2390818298088604 2216 16 0.07322412198001871 2216 17 -0.08001986611825629 2216 18 0.08165435835469026 2216 19 0.020446144951726808 2216 20 0.16590310142528608 2216 21 0.0976623470845368 2216 22 0.15020847107692958 2216 23 0.07301327937275914 2216 24 -0.02973401809639853 2216 25 0.11518321372584239 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 265512.0 3 9.0 4 2290.0 5 12490.0 6 16235.0 7 15953.0 8 13693.0 9 13025.0 10 13052.0 11 15398.0 12 25655.0 13 21832.0 14 10151.0 15 10267.0 16 10946.0 17 11714.0 18 12990.0 19 15622.0 20 19598.0 21 24646.0 22 30544.0 23 36838.0 24 43207.0 25 49875.0 26 57635.0 27 64974.0 28 76357.0 29 93108.0 30 118813.0 31 155088.0 32 202921.0 33 272724.0 34 381091.0 35 546490.0 36 832344.0 37 1560841.0 38 4694908.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.227146268075344 17.939742481934413 13.157513586939773 25.67559766305047 2 16.27398842754765 20.69294781916941 38.34896679816387 24.684096955119067 3 18.36660249307024 24.487994988869634 28.735669860549844 28.409732657510283 4 12.94504491828932 16.863544001691263 37.18323358856798 33.00817749145144 5 15.567497342745678 35.444540689877996 33.07549171408344 15.912470253292884 6 32.406254846728174 36.66351309641765 16.697348712026415 14.232883344827767 7 29.222200076481492 30.508837963720346 21.491103741209297 18.77785821858886 8 26.66010144448573 32.93147262825789 19.999681831337764 20.408744095918614 9 27.45755888627226 15.072218998620562 18.9759566988809 38.494265416226284 10 16.58396524826284 26.276366882877937 30.78605163758662 26.353616231272603 11 36.04319609721755 21.827006143957245 23.00504208554937 19.124755673275832 12 25.465861210222705 23.513347569336503 28.594574453597964 22.426216766842824 13 29.500072838054525 20.55224563696956 24.682065247410378 25.265616277565538 14 23.771081223864478 20.812382153647228 25.045161422208217 30.37137520028008 15 24.716699820179148 27.856342786429263 22.563846623767223 24.86311076962437 16 24.892954191724904 26.28755947534286 24.349821261636166 24.46966507129607 17 24.669503237394995 25.661298092459184 24.88660290232972 24.7825957678161 18 26.089526650289198 24.28640077779228 26.03095061481605 23.593121957102476 19 27.220170184980713 24.561471255291305 24.329953684095756 23.888404875632226 20 26.203501811454473 24.953661727669218 24.499690699582715 24.343145761293595 21 26.2010843558211 24.777587295392753 24.252134667432728 24.76919368135343 22 25.41387248192986 25.144647843931484 24.92244149867143 24.51903817546723 23 24.260153027184682 24.519695754233208 25.32837440385538 25.89177681472673 24 24.98082814168633 25.404839030723704 25.060881232350944 24.55345159523902 25 24.88421227804084 24.958293295585325 25.1434442222381 25.014050204135735 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3927.0 1 3927.0 2 4139.0 3 4351.0 4 4351.0 5 4351.0 6 7112.5 7 9874.0 8 9874.0 9 9874.0 10 13837.5 11 17801.0 12 17801.0 13 17801.0 14 25216.0 15 32631.0 16 32631.0 17 32631.0 18 54452.5 19 76274.0 20 76274.0 21 76274.0 22 123949.5 23 171625.0 24 171625.0 25 171625.0 26 258039.0 27 344453.0 28 344453.0 29 344453.0 30 438814.0 31 533175.0 32 533175.0 33 533175.0 34 636443.0 35 739711.0 36 739711.0 37 739711.0 38 807581.5 39 875452.0 40 875452.0 41 875452.0 42 963142.0 43 1050832.0 44 1050832.0 45 1050832.0 46 1103996.5 47 1157161.0 48 1157161.0 49 1157161.0 50 1162908.5 51 1168656.0 52 1168656.0 53 1168656.0 54 1077865.0 55 987074.0 56 987074.0 57 987074.0 58 917027.5 59 846981.0 60 846981.0 61 846981.0 62 746806.0 63 646631.0 64 646631.0 65 646631.0 66 543319.0 67 440007.0 68 440007.0 69 440007.0 70 348990.0 71 257973.0 72 257973.0 73 257973.0 74 211780.5 75 165588.0 76 165588.0 77 165588.0 78 140662.0 79 115736.0 80 115736.0 81 115736.0 82 88286.0 83 60836.0 84 60836.0 85 60836.0 86 44876.0 87 28916.0 88 28916.0 89 28916.0 90 20458.0 91 12000.0 92 12000.0 93 12000.0 94 6499.0 95 998.0 96 998.0 97 998.0 98 585.5 99 173.0 100 173.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14106299459750887 2 0.1594959644412933 3 0.17335402913742728 4 0.19320255259191968 5 0.18388862013885554 6 0.06604891086484582 7 0.05438598002879523 8 0.057196571980490794 9 0.06621303302260906 10 0.06522830007602959 11 0.6885745128956934 12 0.6468977424586895 13 0.6458719789726691 14 0.6464874370642814 15 0.6471028951558935 16 0.6537190696407242 17 0.6458412060680885 18 0.6563655394346567 19 0.6524881534575 20 0.6572784689372146 21 0.6452154903416162 22 0.6487338590986658 23 0.6339115767256727 24 0.6438101943657684 25 0.6374504607524426 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 9748836.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.07990616255325 #Duplication Level Percentage of deduplicated Percentage of total 1 82.70241938679371 38.10919724759578 2 10.754919243393996 9.911713390428668 3 2.726089021946933 3.768537789662437 4 1.0597645217973466 1.9533539887529938 5 0.5833236366265603 1.3439749219075603 6 0.36011933592847745 0.9956559124143138 7 0.2540760480303874 0.8195460315974836 8 0.19256572449575277 0.7098728411910695 9 0.1463362569343477 0.6068844888942622 >10 0.9800611990895023 8.787720816490776 >50 0.11614793772248523 3.7373422403863445 >100 0.09696535078692936 9.042227898294437 >500 0.013158886342029177 4.152666735288082 >1k 0.012843105157128628 12.060893318668557 >5k 0.0010982759773944643 3.3957756207061873 >10k+ 1.1206897728514944E-4 0.6046367577212515 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACAAAGGGCAGGGACTTAATCAA 15428 0.15825479062320874 No Hit GTATCTGATCGTCTTCGAACCTCCG 11296 0.1158702433808508 No Hit CTATTGGAGCTGGAATTACCGCGGC 10829 0.11107992790113609 No Hit GAATAGGACCGCGGTTCTATTTTGT 10651 0.10925406889602 No Hit GATTAAGAGGGACGGCCGGGGGCAT 10338 0.10604342918477652 No Hit GTTCAAAGCAGGCCCGAGCCGCCTG 9913 0.10168393436919033 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0257634860202798E-5 2 0.0 1.0257634860202798E-5 0.0 1.0257634860202798E-5 1.0257634860202798E-5 3 0.0 1.0257634860202798E-5 0.0 1.0257634860202798E-5 1.0257634860202798E-5 4 0.0 1.0257634860202798E-5 0.0 4.103053944081119E-5 2.0515269720405596E-5 5 0.0 3.077290458060839E-5 0.0 4.103053944081119E-5 2.0515269720405596E-5 6 0.0 3.077290458060839E-5 0.0 4.103053944081119E-5 4.103053944081119E-5 7 0.0 3.077290458060839E-5 0.0 5.1288174301013985E-5 4.103053944081119E-5 8 0.0 3.077290458060839E-5 0.0 5.1288174301013985E-5 4.103053944081119E-5 9 0.0 3.077290458060839E-5 0.0 5.1288174301013985E-5 4.103053944081119E-5 10 0.0 3.077290458060839E-5 0.0 8.206107888162238E-5 4.103053944081119E-5 11 0.0 3.077290458060839E-5 0.0 8.206107888162238E-5 4.103053944081119E-5 12 0.0 3.077290458060839E-5 0.0 8.206107888162238E-5 6.154580916121678E-5 13 0.0 4.103053944081119E-5 0.0 8.206107888162238E-5 9.231871374182518E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4710 0.0 15.693626 1 GTATCAA 17230 0.0 14.001602 1 TAGATCG 305 0.0 11.53709 5 ACCGTCC 585 0.0 10.730129 8 CTCGACG 370 0.0 10.53714 13 CGATACT 90 9.895945E-5 10.515947 4 GGTAGCG 510 0.0 10.443281 11 CGAACGA 860 0.0 10.282753 16 ACGCTTA 345 0.0 10.197298 17 GTATTAG 1745 0.0 10.196605 1 CGTCGTA 1570 0.0 10.116631 10 CGCTTAC 360 0.0 10.036633 18 CGCCAGT 1295 0.0 9.912221 18 GGGTAGG 2140 0.0 9.906663 1 AGGCCCG 2055 0.0 9.857956 10 CCGGTTT 580 0.0 9.836721 12 CGCGTAT 235 0.0 9.712651 7 ACGCATC 795 0.0 9.690294 10 CACTCTA 1700 0.0 9.678634 9 ACTCTAA 1760 0.0 9.672961 10 >>END_MODULE