FastQCFastQC Report
Thu 2 Feb 2017
SRR4050281_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050281_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9748836
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA148320.1521412402465279No Hit
GATTAAGAGGGACGGCCGGGGGCAT111090.11395206566199288No Hit
GAATAGGACCGCGGTTCTATTTTGT110650.11350072972814397No Hit
CTATTGGAGCTGGAATTACCGCGGC107020.10977720827389033No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52100.015.5467481
GTATCAA166500.013.3824271
TAGATCG2900.011.7948785
CGCTTAC3300.010.93043418
CGTCGTA16950.010.92378210
AACCGCG9600.010.8813567
ACGCTTA3350.010.76729417
CGGTAGC4650.010.61838510
GCTCGTA9750.010.5180189
CCGTCGT17700.010.4610719
CGGTTCT22950.010.38391812
ATACCGT18400.010.2738926
CGACCAT17100.010.27267910
CCGACCA17950.010.1566759
TACCGTC18300.010.1191767
ACCGTCG19500.010.0310738
GGTAGCG4850.09.984735511
CTCGTAG10200.09.96074210
ACGGTAT16750.09.9206039
GAACCGC11600.09.9089396
Produced by FastQC (version 0.11.5)