FastQCFastQC Report
Thu 2 Feb 2017
SRR4050280_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050280_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467600
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA364727.7998289136013685No Hit
CTTCCGTACTCTGCGTTGATACCAC211734.528015397775877No Hit
GAGTACGGAAGCAGTGGTATCAACG187864.0175363558597095No Hit
TCCGTACTCTGCGTTGATACCACTG168833.6105645851154833No Hit
GCTTCCGTACTCTGCGTTGATACCA156783.3528656971770743No Hit
GTACATGGGAAGCAGTGGTATCAAC106892.285928143712575No Hit
CCCATGTACTCTGCGTTGATACCAC98212.100299401197605No Hit
GCGTTGATACCACTGCTTCCGTACT83101.7771599657827202No Hit
GTACATGGAAGCAGTGGTATCAACG82381.7617621899059026No Hit
GTATCAACGCAGAGTACGGAAGCAG79841.7074422583404618No Hit
TCCATGTACTCTGCGTTGATACCAC63011.347519247219846No Hit
TATCAACGCAGAGTACGGAAGCAGT58611.253421727972626No Hit
ACTCTGCGTTGATACCACTGCTTCC54571.167023096663815No Hit
CAGTGGTATCAACGCAGAGTACGGA39300.8404619332763045No Hit
GGTATCAACGCAGAGTACGGAAGCA38760.8289136013686912No Hit
CATGTACTCTGCGTTGATACCACTG38510.8235671514114628No Hit
ATACCACTGCTTCCGTACTCTGCGT35790.7653977758768178No Hit
GAGTACATGGGAAGCAGTGGTATCA35240.7536355859709154No Hit
ACGCAGAGTACGGAAGCAGTGGTAT34890.7461505560307956No Hit
GTACGGGAAGCAGTGGTATCAACGC32890.7033789563729683No Hit
AAGCAGTGGTATCAACGCAGAGTAC27770.5938836612489307No Hit
GAGTACATGGAAGCAGTGGTATCAA26650.5699315654405475No Hit
GAAGCAGTGGTATCAACGCAGAGTA26540.567579127459367No Hit
GTATCAACGCAGAGTACATGGGAAG25500.5453378956372968No Hit
GTACTCTGCGTTGATACCACTGCTT25380.5427715996578272No Hit
GTGGTATCAACGCAGAGTACGGAAG25310.5412745936698032No Hit
GCGTTGATACCACTGCTTCCCATGT24790.5301539777587682No Hit
GATACCACTGCTTCCGTACTCTGCG20790.44461077844311375No Hit
GCTTCCCATGTACTCTGCGTTGATA20270.43349016253207867No Hit
CCCGTACTCTGCGTTGATACCACTG19920.4260051325919589No Hit
TTCCCGTACTCTGCGTTGATACCAC19620.4195893926432849No Hit
TATCAACGCAGAGTACATGGGAAGC19070.4078272027373824No Hit
GTTGATACCACTGCTTCCGTACTCT18790.4018391787852866No Hit
ATCAACGCAGAGTACGGAAGCAGTG18750.40098374679213No Hit
CCACTGCTTCCGTACTCTGCGTTGA18340.39221556886227543No Hit
CGGAAGCAGTGGTATCAACGCAGAG17690.3783147989734816No Hit
CTGCTTCCGTACTCTGCGTTGATAC16470.35222412318220697No Hit
GCTTCCATGTACTCTGCGTTGATAC16400.3507271171941831No Hit
CTTCCCGTACTCTGCGTTGATACCA16000.3421727972626176No Hit
CAGTGGTATCAACGCAGAGTACATG15710.33597091531223267No Hit
CATGGAAGCAGTGGTATCAACGCAG15550.3325491873396065No Hit
ACGCAGAGTACATGGGAAGCAGTGG15450.3304106073567151No Hit
GAGTACGGGAAGCAGTGGTATCAAC15290.32698887938408894No Hit
AAAAAGTACTCTGCGTTGATACCAC13910.2974764756201882No Hit
GCGTTGATACCACTGCTTCCATGTA13320.2848588537211291No Hit
GTATCAACGCAGAGTACGGGAAGCA12940.276732249786142No Hit
GTATCAACGCAGAGTACATGGAAGC12310.26325919589392643No Hit
GCAGTGGTATCAACGCAGAGTACGG11570.24743370402053036No Hit
GCAGAGTACGGAAGCAGTGGTATCA11460.24508126603934988No Hit
GGTATCAACGCAGAGTACATGGGAA11440.2446535500427716No Hit
GCTTCCCGTACTCTGCGTTGATACC11300.2416595380667237No Hit
CCATGTACTCTGCGTTGATACCACT11180.23909324208725405No Hit
CACTGCTTCCGTACTCTGCGTTGAT10710.22904191616766467No Hit
TGATACCACTGCTTCCGTACTCTGC10080.2155688622754491No Hit
GCGTTGATACCACTGCTTCCCGTAC10020.2142857142857143No Hit
TATCAACGCAGAGTACATGGAAGCA9840.21043627031650985No Hit
CCCCATGTACTCTGCGTTGATACCA9130.19525235243798117No Hit
AAAGTACTCTGCGTTGATACCACTG9120.19503849443969204No Hit
GGGAAGCAGTGGTATCAACGCAGAG9040.19332763045337895No Hit
GCCCATGTACTCTGCGTTGATACCA8980.19204448246364414No Hit
ATACCACTGCTTCCCATGTACTCTG8730.18669803250641573No Hit
CTGCGTTGATACCACTGCTTCCGTA8730.18669803250641573No Hit
TACCACTGCTTCCGTACTCTGCGTT8700.18605645851154834No Hit
ACGCAGAGTACATGGAAGCAGTGGT8540.18263473053892215No Hit
CTGCTTCCCATGTACTCTGCGTTGA8500.1817792985457656No Hit
TATCAACGCAGAGTACGGGAAGCAG7930.16958939264328485No Hit
TACGGAAGCAGTGGTATCAACGCAG7720.165098374679213No Hit
TCCCATGTACTCTGCGTTGATACCA7690.1644568006843456No Hit
GCAGTGGTATCAACGCAGAGTACAT7500.16039349871685202No Hit
CATGGGAAGCAGTGGTATCAACGCA7340.15697177074422583No Hit
GTGGTATCAACGCAGAGTACATGGG7220.1544054747647562No Hit
ACGGAAGCAGTGGTATCAACGCAGA7060.15098374679213003No Hit
TCAACGCAGAGTACGGAAGCAGTGG6960.14884516680923868No Hit
GTATCAACGCAGAGTACTTTTTTTT6700.14328485885372114No Hit
TACCACTGCTTCCCATGTACTCTGC6650.14221556886227546No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6630.14178785286569717No Hit
CTCTGCGTTGATACCACTGCTTCCG6480.13857998289136014No Hit
CTTCCCATGTACTCTGCGTTGATAC6290.13451668092386654No Hit
ATACCACTGCTTCCATGTACTCTGC6200.13259195893926431No Hit
GCAGAGTACATGGGAAGCAGTGGTA5590.11954662104362702No Hit
GGTATCAACGCAGAGTACATGGAAG5550.11869118905047048No Hit
AGCAGTGGTATCAACGCAGAGTACG5400.11548331907613345No Hit
CCACTGCTTCCCATGTACTCTGCGT5180.11077844311377245No Hit
GATACCACTGCTTCCCATGTACTCT5170.11056458511548332No Hit
GGTATCAACGCAGAGTACGGGAAGC5130.10970915312232678No Hit
GTTGATACCACTGCTTCCCATGTAC5020.1073567151411463No Hit
ACATGGGAAGCAGTGGTATCAACGC4930.10543199315654406No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGCAG250.00597279619.0291088
TACTTGG350.002239487116.205842
TCTTCGA456.699121E-414.79244412
GGGCATT456.7045976E-414.79085119
GGGACGG400.005218449514.2718318
TTAACTG400.005218449514.2718315
TACACAG400.005218449514.2718315
CGAACCT400.005240891614.26260516
CGGGGCA400.005446548614.1801094
TGTAGAA400.005446548614.1801092
GTACAAA955.0840754E-913.9567491
GAGTATG552.0075175E-413.7754931
CCACCCG552.0075175E-413.7754931
AAAAAGT2450.013.5295021
AAAAGTA2550.013.3459852
TGCCCGT500.00155329513.2347682
GCCCATG1900.012.9598381
AGTATGG604.255354E-412.6045422
ACACTGT853.8598664E-612.3129526
CGTCTTC550.003027953212.10943310
Produced by FastQC (version 0.11.5)