##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050280_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	467600
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.59325491873396	31.0	31.0	34.0	27.0	34.0
2	30.68113130881095	31.0	31.0	34.0	27.0	34.0
3	30.828954234388366	31.0	31.0	34.0	27.0	34.0
4	34.288378956372966	37.0	35.0	37.0	30.0	37.0
5	34.30461719418306	37.0	35.0	37.0	30.0	37.0
6	34.30498930710009	37.0	35.0	37.0	30.0	37.0
7	34.171849871685204	37.0	35.0	37.0	30.0	37.0
8	34.10205731394354	37.0	35.0	37.0	30.0	37.0
9	35.537010265183916	39.0	37.0	39.0	30.0	39.0
10	35.550930282292555	39.0	37.0	39.0	30.0	39.0
11	35.37686911890505	39.0	37.0	39.0	30.0	39.0
12	35.34421514114628	39.0	37.0	39.0	30.0	39.0
13	35.28686056458512	39.0	37.0	39.0	30.0	39.0
14	36.47108212147134	40.0	37.0	41.0	30.0	41.0
15	36.39624465355004	40.0	37.0	41.0	29.0	41.0
16	36.224253635585974	40.0	37.0	41.0	29.0	41.0
17	36.32607142857143	40.0	37.0	41.0	30.0	41.0
18	36.213830196749356	40.0	37.0	41.0	29.0	41.0
19	36.31693541488452	40.0	37.0	41.0	29.0	41.0
20	36.351565440547475	40.0	37.0	41.0	30.0	41.0
21	36.30165953806672	40.0	37.0	41.0	29.0	41.0
22	36.28731394354149	40.0	37.0	41.0	30.0	41.0
23	36.18210650128315	40.0	37.0	41.0	29.0	41.0
24	36.082258340461934	40.0	37.0	41.0	29.0	41.0
25	35.827097946963214	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.7086403870092006
1101	2	0.6824606956120292
1101	3	0.5451176479951023
1101	4	0.5007718937309278
1101	5	0.6571563707878028
1101	6	0.7490395230107154
1101	7	0.6794320831950813
1101	8	0.7405787207383625
1101	9	0.7234399416981177
1101	10	0.8956454058291783
1101	11	0.7856001795867584
1101	12	0.7197648635122604
1101	13	0.8416799460426176
1101	14	0.7167794743814895
1101	15	0.9932952709257563
1101	16	0.8676220423550802
1101	17	0.7949746303181584
1101	18	0.7865385785311574
1101	19	0.8251690460063941
1101	20	0.7648826135837155
1101	21	0.7699875836542915
1101	22	0.8002389793307572
1101	23	0.9810406774972691
1101	24	0.8308992923771967
1101	25	0.8366153800217262
1102	1	0.5911760217665751
1102	2	0.524427063352217
1102	3	0.5677331401730363
1102	4	0.6105515987919361
1102	5	0.6975033625959455
1102	6	0.603463405875722
1102	7	0.5575646481297554
1102	8	0.43504123907607095
1102	9	0.5476242927151276
1102	10	0.39497251815090095
1102	11	0.6970538190402067
1102	12	0.5596842786455625
1102	13	0.35592980762577753
1102	14	0.6330645686059668
1102	15	0.7163504638808433
1102	16	0.7455020218249331
1102	17	0.819119780071027
1102	18	0.8075173168970764
1102	19	0.9056771267205832
1102	20	0.7185382324989291
1102	21	0.7733196911061739
1102	22	0.7112258408452163
1102	23	0.6923880289871249
1102	24	0.6887199445730801
1102	25	0.6171691281879532
1103	1	0.4097346644374511
1103	2	0.38329348748537484
1103	3	0.4664204007248536
1103	4	0.4876362064993458
1103	5	0.6911851500464508
1103	6	0.6323179179648335
1103	7	0.4021325950728638
1103	8	0.32571604027371137
1103	9	0.5343640950395852
1103	10	0.38688762069106275
1103	11	0.45789538885443903
1103	12	0.5707874305080267
1103	13	0.6432837241126919
1103	14	0.8285387967377105
1103	15	0.6407572762822511
1103	16	0.8785667975887534
1103	17	0.8985803576441853
1103	18	0.8330235787688736
1103	19	0.9440934687572238
1103	20	0.9121049238482115
1103	21	1.0128403118202485
1103	22	0.9291196626796747
1103	23	1.0605144576275407
1103	24	1.0603073049285072
1103	25	1.0531016480681927
1104	1	0.16153490033043028
1104	2	0.25699762802792847
1104	3	0.24655107301984458
1104	4	0.2414306692220478
1104	5	0.2015520257060146
1104	6	0.1170935092492087
1104	7	0.3366334951908101
1104	8	0.250369960442562
1104	9	0.1404150603384906
1104	10	0.16749939050917817
1104	11	0.3137368439416619
1104	12	0.3076658835466972
1104	13	0.4186267000888577
1104	14	0.42515404287340886
1104	15	0.3219758497505012
1104	16	0.39381448521678664
1104	17	0.3127044497666063
1104	18	0.23610570677185194
1104	19	0.6115930031789745
1104	20	0.5136552994146655
1104	21	0.544235805022808
1104	22	0.6480500742508468
1104	23	0.5182321930549421
1104	24	0.6826845474637366
1104	25	0.796133493620367
1105	1	0.4502089334796011
1105	2	0.5732959705693652
1105	3	0.6056670951192906
1105	4	0.6834196194982951
1105	5	0.5206127991866794
1105	6	0.6972040064867784
1105	7	0.5300036609366714
1105	8	0.5680495184536127
1105	9	0.7301940658633512
1105	10	0.7973336446528236
1105	11	0.9380462362068513
1105	12	0.9319752758118938
1105	13	1.0249802912490793
1105	14	1.0688004517131873
1105	15	0.9269482254411088
1105	16	1.1631515017913685
1105	17	1.094472405567707
1105	18	1.0524040493132887
1105	19	1.008001842957981
1105	20	1.0302298850500193
1105	21	0.9820811088902133
1105	22	1.0306467593337132
1105	23	1.1314918615632266
1105	24	1.1964967021598696
1105	25	1.1980671952778223
1106	1	0.09467942886007208
1106	2	0.23053013495334795
1106	3	0.06791774123409056
1106	4	0.07651276836004683
1106	5	0.1310790851485777
1106	6	0.056915847024100685
1106	7	0.18214628594247273
1106	8	0.1831972349987936
1106	9	0.17857065406769124
1106	10	-0.048415152103444825
1106	11	0.0803578569877601
1106	12	0.22659975543800215
1106	13	-0.04407661328069423
1106	14	0.2213841371210563
1106	15	0.43352157301217886
1106	16	0.41195599596473187
1106	17	0.2762241117750577
1106	18	0.2377461419514475
1106	19	0.3258090763485768
1106	20	0.4227310844247967
1106	21	0.39162890590957034
1106	22	0.31369146893026567
1106	23	0.16074499928240016
1106	24	0.41432250651475755
1106	25	0.4933339207525478
1107	1	0.38281776111605126
1107	2	0.433315095091249
1107	3	0.5210259075775525
1107	4	0.3566786808789004
1107	5	0.5437595419466845
1107	6	0.5365807899243364
1107	7	0.4370751805772457
1107	8	0.4368871725122574
1107	9	0.30594083290846186
1107	10	0.6953361945890748
1107	11	0.5423811278345596
1107	12	0.7672160019365961
1107	13	0.700698219729432
1107	14	0.8009736652399937
1107	15	0.772995824251133
1107	16	0.6515325958354481
1107	17	0.6748181647808735
1107	18	0.9316159975860145
1107	19	0.837444499748706
1107	20	0.8924574365040954
1107	21	0.9120716984111894
1107	22	0.831144604030456
1107	23	0.7919936525898805
1107	24	0.8819210844074519
1107	25	0.81629772165752
1108	1	0.060352490537574965
1108	2	0.04219442331020318
1108	3	0.18210303799430605
1108	4	-0.06510236826063931
1108	5	0.14571890334000415
1108	6	0.16256565411674728
1108	7	-0.15589081569063268
1108	8	0.012540292976702005
1108	9	0.05384151712989649
1108	10	0.12935029369751305
1108	11	0.1352175367087085
1108	12	-0.05623413283429102
1108	13	-4.2141535246287276E-4
1108	14	-0.0146234734703512
1108	15	-0.07958555663213218
1108	16	-0.07884787275256855
1108	17	0.22548683573256056
1108	18	0.24350693683544478
1108	19	0.03327696916608147
1108	20	0.3916280771841656
1108	21	0.2929290486081584
1108	22	-0.01998539804294097
1108	23	0.06653750496391808
1108	24	-0.045484057620328144
1108	25	-0.021173769115854668
1109	1	0.30395396436234634
1109	2	0.14756300314649806
1109	3	0.35693426527490146
1109	4	0.20936040282667534
1109	5	0.35979442449532684
1109	6	0.48966325791506193
1109	7	0.5577148619967502
1109	8	0.455021561059489
1109	9	0.6183337152554671
1109	10	0.4903508129981873
1109	11	0.6442311127120703
1109	12	0.5372028957767014
1109	13	0.5800207320794684
1109	14	0.47340788239898046
1109	15	0.4518444985126564
1109	16	0.5927439074596066
1109	17	0.621725615128689
1109	18	0.5398174291819373
1109	19	0.4688589568512356
1109	20	0.5732720347498841
1109	21	0.6735269862426208
1109	22	0.7147014141927741
1109	23	0.6606422058714614
1109	24	0.5623450182717562
1109	25	0.48600989022290264
1110	1	0.1837269035200393
1110	2	0.23079725210948965
1110	3	0.08241426149167808
1110	4	-0.002503374179440243
1110	5	0.06450554851743107
1110	6	-0.018180170764459547
1110	7	0.04872126645013708
1110	8	0.043152041663724106
1110	9	-0.039306703060724146
1110	10	0.003127629957830891
1110	11	-0.027900394766547265
1110	12	-0.041325971553831664
1110	13	-0.16832579749046062
1110	14	-0.08031386166609167
1110	15	0.08931223979435288
1110	16	-0.0674687636298259
1110	17	0.20580264421896288
1110	18	0.02645286175277306
1110	19	-0.1751871984500042
1110	20	-0.09461348226786015
1110	21	-0.053446031281801254
1110	22	-0.05709602132357361
1110	23	-0.11723371797769744
1110	24	0.20790952895259807
1110	25	0.10519825411475381
1111	1	-0.38235352991730664
1111	2	-0.5165694711726267
1111	3	-0.46952712176858213
1111	4	-0.581004644735458
1111	5	-0.6217247183651651
1111	6	-0.5710234513005972
1111	7	-0.9299146303780788
1111	8	-0.7789125131150101
1111	9	-0.5822340509560817
1111	10	-0.7384244891716065
1111	11	-0.7393508694146789
1111	12	-0.6132802578700094
1111	13	-0.668366942439178
1111	14	-0.6900425025003116
1111	15	-0.7625675906226235
1111	16	-0.7570449377420871
1111	17	-0.8662520357720851
1111	18	-0.7008170227780397
1111	19	-0.5414167411815001
1111	20	-0.5970377426462576
1111	21	-0.7513301934389816
1111	22	-0.6899843308915834
1111	23	-0.9515627590699793
1111	24	-0.716546699405157
1111	25	-0.4493665310756967
1112	1	0.09880866676213174
1112	2	0.12387022961190297
1112	3	0.14185766202999517
1112	4	0.2485884952025046
1112	5	0.2567189962854499
1112	6	0.16328598128470162
1112	7	0.25371974291146415
1112	8	0.16817743397493246
1112	9	0.0801450769402905
1112	10	0.2830588167944583
1112	11	0.44177483757309943
1112	12	0.4679549181927527
1112	13	0.19037233871389958
1112	14	0.2787588111285899
1112	15	0.6440644108369611
1112	16	0.47092441091691484
1112	17	0.33932176462929675
1112	18	0.2056781423378098
1112	19	0.33479197985480624
1112	20	0.44086592456775264
1112	21	0.28347184998375496
1112	22	0.2249490754211365
1112	23	0.15439418309385644
1112	24	0.13954707910241382
1112	25	0.20740230672762294
1113	1	-0.5029496972320509
1113	2	-0.3318834160750015
1113	3	-0.30399181663371877
1113	4	-0.1630838146900473
1113	5	-0.32127058921248164
1113	6	-0.2638889016743846
1113	7	-0.12181351137596153
1113	8	-0.31156195900138783
1113	9	-0.264291354259214
1113	10	-0.21967621235881296
1113	11	-0.0897528672026624
1113	12	-0.11190487718427988
1113	13	-0.2629671471617385
1113	14	-0.20607170874086478
1113	15	-0.16621013108385085
1113	16	-0.38511221097064663
1113	17	-0.2639889958004815
1113	18	-0.128912183909911
1113	19	-0.15564305785498078
1113	20	-0.33281728877063443
1113	21	-0.42960678439632716
1113	22	-0.34574302447007454
1113	23	-0.3296387472683264
1113	24	-0.4049009645113699
1113	25	-0.40556509294722787
1114	1	-0.5428169490014163
1114	2	-0.46110513984622514
1114	3	-0.5613443084133536
1114	4	-0.698455839772862
1114	5	-0.5486997229263721
1114	6	-0.8046943142818037
1114	7	-0.7438172029013401
1114	8	-0.6729555709511743
1114	9	-0.793007632419318
1114	10	-0.6910791142400257
1114	11	-0.37965373385546997
1114	12	-0.49017316062497684
1114	13	-0.5953419470003851
1114	14	-0.9096648341916094
1114	15	-0.7660341113305265
1114	16	-0.7027209092801243
1114	17	-0.7912201072918847
1114	18	-0.8805287542139126
1114	19	-0.8893459511796777
1114	20	-1.006178410407614
1114	21	-0.9012572140556543
1114	22	-0.8009129935443795
1114	23	-0.7791033538519514
1114	24	-1.2446893740995577
1114	25	-1.2802824223364127
1115	1	-0.6257506189846929
1115	2	-0.655865374373235
1115	3	-0.4423579674641829
1115	4	-0.5582000335374602
1115	5	-0.41595346631321917
1115	6	-0.62968243826095
1115	7	-0.8436798965780952
1115	8	-0.7258884600454039
1115	9	-0.8326393781618435
1115	10	-0.7764400476627245
1115	11	-0.7008106209714526
1115	12	-0.8273301388446797
1115	13	-0.6736830133043767
1115	14	-0.6766654393806135
1115	15	-0.8998524635244962
1115	16	-1.1143031206277456
1115	17	-1.1808821124847952
1115	18	-0.8917214971517495
1115	19	-1.0615059553444226
1115	20	-1.1318722628586286
1115	21	-1.2569895903660893
1115	22	-1.0594588479996787
1115	23	-1.2813565441090589
1115	24	-1.1262789388189915
1115	25	-1.474862076272423
1116	1	-0.44181115902435053
1116	2	-0.39852608043468507
1116	3	-0.4065446950974341
1116	4	-0.12685500881345035
1116	5	-0.6508136301838974
1116	6	-0.5805846646628297
1116	7	-0.4294307039348979
1116	8	-0.8008878395320664
1116	9	-0.6989256471667886
1116	10	-0.5224653149660838
1116	11	-0.24367154600516727
1116	12	-0.6069265569658739
1116	13	-0.6159642409831321
1116	14	-0.5530233294146214
1116	15	-0.6179629038683458
1116	16	-0.466625081560224
1116	17	-0.24502112953650368
1116	18	-0.5609796113979044
1116	19	-0.47550142697593145
1116	20	-0.5949820551107621
1116	21	-0.7022203248056798
1116	22	-0.7630878858179031
1116	23	-0.9191863469512853
1116	24	-0.7806916160444928
1116	25	-0.892536502573364
1201	1	0.6350155633138534
1201	2	0.4950283818045378
1201	3	0.5729989345505793
1201	4	0.41726809146830846
1201	5	0.6815717297588719
1201	6	0.7697226480661925
1201	7	0.6879992565013282
1201	8	0.68496409558918
1201	9	0.5678539198215447
1201	10	0.47680472605760826
1201	11	0.6560174914709265
1201	12	0.7377454724660666
1201	13	0.6297691488003636
1201	14	0.7337658730322048
1201	15	0.6604084116637239
1201	16	0.6768322961351458
1201	17	0.8824355473982521
1201	18	1.0301674461374617
1201	19	0.7809010432052474
1201	20	0.7545952986419522
1201	21	0.6648439261834156
1201	22	0.8443314172172691
1201	23	0.7728877584839893
1201	24	1.0304475579339254
1201	25	1.0719670523268547
1202	1	0.6408979162550317
1202	2	0.7571407792110527
1202	3	0.5721194219063612
1202	4	0.6437396591163491
1202	5	0.6899026145603457
1202	6	0.5961315332986103
1202	7	0.46926113188084884
1202	8	0.4703120809371697
1202	9	0.6379544075690902
1202	10	0.6448621588209207
1202	11	0.7750357281362241
1202	12	0.7111935929730109
1202	13	0.765447196243116
1202	14	0.9020564060286205
1202	15	0.7514487438805233
1202	16	0.7158775645921835
1202	17	0.5003137476293986
1202	18	0.7755612680018729
1202	19	0.7977978718667842
1202	20	0.8050840256012677
1202	21	0.7417689619319816
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2210	18	-0.33186661862742284
2210	19	-0.41997558341910235
2210	20	-0.2657471677343253
2210	21	-0.2843390105157795
2210	22	-0.326859940189955
2210	23	-0.2143993367354895
2210	24	-0.3202449225374835
2210	25	-0.10517336252487297
2211	1	-0.024754551628674903
2211	2	0.045371780423799635
2211	3	-0.07744538485925645
2211	4	0.04096214964512512
2211	5	-0.1632317426308063
2211	6	-0.08698769140276141
2211	7	-0.1061598411060487
2211	8	-0.01007470979445202
2211	9	0.16361524661735416
2211	10	0.05212468068791054
2211	11	0.22744351117280814
2211	12	0.29093430151696964
2211	13	0.1438935939592909
2211	14	0.1962921079722335
2211	15	0.3843034183173515
2211	16	0.4230316906104079
2211	17	0.31893314941309825
2211	18	0.3540999631734252
2211	19	0.1891068858938496
2211	20	0.11816021393100584
2211	21	0.3138039061778741
2211	22	0.4185725018031832
2211	23	0.14039126391852363
2211	24	0.28856185742753837
2211	25	0.3233026898693865
2212	1	-0.24401256967847473
2212	2	-0.2043742615878763
2212	3	-0.08291650550808427
2212	4	-0.03790691255579759
2212	5	-0.26795879087139696
2212	6	-0.16473803191878034
2212	7	-0.0076600713830643485
2212	8	-0.34122122803859867
2212	9	-0.02737259279626869
2212	10	-0.014923494570865614
2212	11	-0.010983027191997508
2212	12	-0.4205808559369828
2212	13	-0.3287557023076033
2212	14	-0.4755468091705026
2212	15	-0.09047063256570453
2212	16	-0.16952007272361413
2212	17	-0.19820044065461673
2212	18	-0.2142694468927857
2212	19	-0.10824280527364039
2212	20	-0.2253020418691989
2212	21	-0.20720193077292492
2212	22	-0.17900126033535457
2212	23	-0.22495710764732735
2212	24	-0.37212250627296584
2212	25	-0.2775831417246195
2213	1	-0.3243829329267456
2213	2	-0.26099279000610665
2213	3	-0.2702069823395483
2213	4	-0.1505991290304891
2213	5	-0.28547701097407696
2213	6	-0.26366501690041844
2213	7	-0.5465135990641627
2213	8	-0.4632718789625798
2213	9	-0.7282552341823418
2213	10	-0.5989090338666756
2213	11	-0.3468130113595791
2213	12	-0.5372952762588881
2213	13	-0.35337928171648514
2213	14	-0.5296847114710133
2213	15	-0.6418875380006455
2213	16	-0.4571284799308941
2213	17	-0.3164340041028737
2213	18	-0.2624749991365647
2213	19	-0.5979709619825471
2213	20	-0.6214234194820278
2213	21	-0.7170250820013848
2213	22	-0.6170913705213081
2213	23	-0.5682097405082516
2213	24	-0.3908521949447703
2213	25	-0.5045283820633202
2214	1	-0.39344695256924567
2214	2	-0.4422957933849503
2214	3	-0.415293332267737
2214	4	-0.4448035091039273
2214	5	-0.4483826868537406
2214	6	-0.4094742668359288
2214	7	-0.29181076828964336
2214	8	-0.26061307184313165
2214	9	-0.2081637093091686
2214	10	-0.3319583025275321
2214	11	-0.2658243261777571
2214	12	-0.1415696215885447
2214	13	-0.02453461604977747
2214	14	-0.09149425438802439
2214	15	-0.1543328826201389
2214	16	-0.14558086884472488
2214	17	-0.04625888396454059
2214	18	-0.17085618080738385
2214	19	-0.20973703536167676
2214	20	0.11098097141898933
2214	21	-0.043621433107865926
2214	22	-0.05257015643144314
2214	23	-0.2194439250802276
2214	24	-0.12361007439562144
2214	25	-0.16771270882686196
2215	1	-0.9549048610893287
2215	2	-1.012639812070553
2215	3	-1.0947850056471538
2215	4	-1.008895400212424
2215	5	-1.1327277638509656
2215	6	-0.9891391797611533
2215	7	-0.9366932923291529
2215	8	-0.8160969123683799
2215	9	-0.8419845176031018
2215	10	-0.9631518690714032
2215	11	-1.1532422143405014
2215	12	-1.0031515615344162
2215	13	-1.2456829852724596
2215	14	-1.1352237580263917
2215	15	-1.3012747327780403
2215	16	-1.307590177942501
2215	17	-1.2520954388568697
2215	18	-1.382612876987693
2215	19	-1.4067767961967306
2215	20	-1.6166369174178499
2215	21	-1.6707721818464876
2215	22	-1.6660134103689401
2215	23	-1.6439195721164452
2215	24	-1.7233744820950108
2215	25	-2.016072494333386
2216	1	-0.6139206068989118
2216	2	-0.7042871962344464
2216	3	-0.760731165136967
2216	4	-0.6259844007203483
2216	5	-0.8794459759396887
2216	6	-0.6457958759629676
2216	7	-0.5372350836266335
2216	8	-0.15988042159575855
2216	9	-0.6221922042671295
2216	10	-0.5396065222685351
2216	11	-0.44428008329473556
2216	12	-0.5914466120451678
2216	13	-0.872016299793593
2216	14	-0.4448236225785678
2216	15	-0.7445164507079483
2216	16	-1.1376595949524884
2216	17	-0.8762164389133034
2216	18	-0.9916596629236878
2216	19	-0.7621580253857374
2216	20	-1.0815897928622888
2216	21	-0.9766528748878542
2216	22	-1.1064050803277397
2216	23	-1.2954800439263678
2216	24	-1.1583933883538293
2216	25	-1.079721684468268
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	11373.0
3	0.0
4	94.0
5	678.0
6	1243.0
7	1366.0
8	1237.0
9	1093.0
10	901.0
11	1269.0
12	1385.0
13	1160.0
14	561.0
15	498.0
16	503.0
17	589.0
18	678.0
19	916.0
20	1188.0
21	1521.0
22	1946.0
23	2187.0
24	2314.0
25	2777.0
26	2996.0
27	3313.0
28	3644.0
29	4320.0
30	5416.0
31	6991.0
32	9408.0
33	12590.0
34	17598.0
35	25870.0
36	40933.0
37	78971.0
38	218073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.394602770600606	12.43920832637041	13.664897013013167	21.50129189001581
2	7.979370814108447	21.903001756176028	40.967458336274554	29.15016909344097
3	22.238646962682797	32.28588175898274	26.527215628556515	18.948255649777945
4	13.503654320142408	13.460863686156374	31.38008352731754	41.65539846638368
5	18.268652757187382	31.780443297800197	27.72697031346601	22.22393363154641
6	38.92844226740366	26.452329440767304	13.16196627013424	21.457262021694795
7	26.323076857229683	32.925299925954775	21.288215681323056	19.463407535492486
8	26.189467286439474	34.51894844072353	25.026751243397793	14.264833029439208
9	29.84445780631798	23.306935311651436	13.21520200799002	33.633404874040565
10	13.231700793803626	25.365770160686395	29.012773605494573	32.389755440015406
11	29.366587404912792	27.85250858071249	16.374043748184334	26.406860266190378
12	27.775268720079488	22.77399985375778	23.95017441535371	25.500557010809022
13	32.71547470334401	19.4822199909267	28.005529981659897	19.796775324069397
14	35.67859937570405	18.309026493881728	21.900018058147232	24.112356072266987
15	38.15349552242015	15.442006471796086	26.90439587611133	19.500102129672435
16	28.967709761875078	19.299554187499194	34.25404863956253	17.478687411063202
17	25.087439834729576	29.7706034477199	28.986385827330242	16.15557089022028
18	27.728467724426427	16.00676702558271	41.48070602346963	14.784059226521236
19	23.646007576767445	36.329997892953436	17.846469406973895	22.177525123305227
20	17.12536385486222	31.38954935742264	30.20995016746998	21.275136620245163
21	18.186215911729548	36.43048185828911	20.629047377428428	24.75425485255291
22	17.112436262328348	33.453141525576754	15.417422633429926	34.01699957866497
23	23.450839426494017	25.805834384990295	16.253421020383154	34.48990516813254
24	15.964276886371698	30.637406097862417	17.77149673827768	35.6268202774882
25	27.159525211836172	30.175960517434834	16.636071691121153	26.02844257960784
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	204.0
1	204.0
2	252.5
3	301.0
4	301.0
5	301.0
6	698.5
7	1096.0
8	1096.0
9	1096.0
10	1102.5
11	1109.0
12	1109.0
13	1109.0
14	1221.0
15	1333.0
16	1333.0
17	1333.0
18	2032.0
19	2731.0
20	2731.0
21	2731.0
22	3078.5
23	3426.0
24	3426.0
25	3426.0
26	4614.0
27	5802.0
28	5802.0
29	5802.0
30	7003.5
31	8205.0
32	8205.0
33	8205.0
34	9308.0
35	10411.0
36	10411.0
37	10411.0
38	12093.5
39	13776.0
40	13776.0
41	13776.0
42	18576.5
43	23377.0
44	23377.0
45	23377.0
46	62473.0
47	101569.0
48	101569.0
49	101569.0
50	159609.5
51	217650.0
52	217650.0
53	217650.0
54	130677.0
55	43704.0
56	43704.0
57	43704.0
58	28038.5
59	12373.0
60	12373.0
61	12373.0
62	10221.0
63	8069.0
64	8069.0
65	8069.0
66	6621.0
67	5173.0
68	5173.0
69	5173.0
70	4080.0
71	2987.0
72	2987.0
73	2987.0
74	2453.5
75	1920.0
76	1920.0
77	1920.0
78	1625.5
79	1331.0
80	1331.0
81	1331.0
82	975.0
83	619.0
84	619.0
85	619.0
86	464.5
87	310.0
88	310.0
89	310.0
90	210.0
91	110.0
92	110.0
93	110.0
94	60.0
95	10.0
96	10.0
97	10.0
98	7.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.18006843455945254
2	0.02288280581693755
3	0.03165098374679213
4	0.04448246364414029
5	0.031864841745081265
6	0.06394354148845166
7	0.06800684345594525
8	0.07100085543199315
9	0.057100085543199314
10	0.04918733960650128
11	0.6191189050470488
12	0.5598802395209581
13	0.5344311377245509
14	0.5209580838323353
15	0.5357142857142857
16	0.5094097519247219
17	0.5181779298545766
18	0.5139007698887939
19	0.5333618477331052
20	0.5222412318220702
21	0.5312232677502139
22	0.5158254918733961
23	0.5263045337895637
24	0.5036355859709153
25	0.5094097519247219
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	467600.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.630321453441624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.61669291636278	19.168075539218503
2	5.516039243086726	2.386274039555294
3	1.5939971551755767	1.0343601258695758
4	0.8978534278523995	0.7768343305008744
5	0.5579039249061096	0.6033820617927954
6	0.39393196620520277	0.5112525035882903
7	0.2559557882406954	0.3875484184260692
8	0.2059644230347052	0.3564061342570648
9	0.19696597735141427	0.3834393664952154
>10	1.4037575238579414	6.097399604575486
>50	0.15997236739121362	2.3877913397927553
>100	0.1149801390624348	5.289646624399149
>500	0.03099464618204764	4.859926364262549
>1k	0.041992746440193573	18.634980198887995
>5k	0.0069987910733655965	11.239769179576948
>10k+	0.005998963777170511	25.88291416880144
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	36472	7.7998289136013685	No Hit
CTTCCGTACTCTGCGTTGATACCAC	21173	4.528015397775877	No Hit
GAGTACGGAAGCAGTGGTATCAACG	18786	4.0175363558597095	No Hit
TCCGTACTCTGCGTTGATACCACTG	16883	3.6105645851154833	No Hit
GCTTCCGTACTCTGCGTTGATACCA	15678	3.3528656971770743	No Hit
GTACATGGGAAGCAGTGGTATCAAC	10689	2.285928143712575	No Hit
CCCATGTACTCTGCGTTGATACCAC	9821	2.100299401197605	No Hit
GCGTTGATACCACTGCTTCCGTACT	8310	1.7771599657827202	No Hit
GTACATGGAAGCAGTGGTATCAACG	8238	1.7617621899059026	No Hit
GTATCAACGCAGAGTACGGAAGCAG	7984	1.7074422583404618	No Hit
TCCATGTACTCTGCGTTGATACCAC	6301	1.347519247219846	No Hit
TATCAACGCAGAGTACGGAAGCAGT	5861	1.253421727972626	No Hit
ACTCTGCGTTGATACCACTGCTTCC	5457	1.167023096663815	No Hit
CAGTGGTATCAACGCAGAGTACGGA	3930	0.8404619332763045	No Hit
GGTATCAACGCAGAGTACGGAAGCA	3876	0.8289136013686912	No Hit
CATGTACTCTGCGTTGATACCACTG	3851	0.8235671514114628	No Hit
ATACCACTGCTTCCGTACTCTGCGT	3579	0.7653977758768178	No Hit
GAGTACATGGGAAGCAGTGGTATCA	3524	0.7536355859709154	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	3489	0.7461505560307956	No Hit
GTACGGGAAGCAGTGGTATCAACGC	3289	0.7033789563729683	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	2777	0.5938836612489307	No Hit
GAGTACATGGAAGCAGTGGTATCAA	2665	0.5699315654405475	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	2654	0.567579127459367	No Hit
GTATCAACGCAGAGTACATGGGAAG	2550	0.5453378956372968	No Hit
GTACTCTGCGTTGATACCACTGCTT	2538	0.5427715996578272	No Hit
GTGGTATCAACGCAGAGTACGGAAG	2531	0.5412745936698032	No Hit
GCGTTGATACCACTGCTTCCCATGT	2479	0.5301539777587682	No Hit
GATACCACTGCTTCCGTACTCTGCG	2079	0.44461077844311375	No Hit
GCTTCCCATGTACTCTGCGTTGATA	2027	0.43349016253207867	No Hit
CCCGTACTCTGCGTTGATACCACTG	1992	0.4260051325919589	No Hit
TTCCCGTACTCTGCGTTGATACCAC	1962	0.4195893926432849	No Hit
TATCAACGCAGAGTACATGGGAAGC	1907	0.4078272027373824	No Hit
GTTGATACCACTGCTTCCGTACTCT	1879	0.4018391787852866	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	1875	0.40098374679213	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	1834	0.39221556886227543	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	1769	0.3783147989734816	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	1647	0.35222412318220697	No Hit
GCTTCCATGTACTCTGCGTTGATAC	1640	0.3507271171941831	No Hit
CTTCCCGTACTCTGCGTTGATACCA	1600	0.3421727972626176	No Hit
CAGTGGTATCAACGCAGAGTACATG	1571	0.33597091531223267	No Hit
CATGGAAGCAGTGGTATCAACGCAG	1555	0.3325491873396065	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1545	0.3304106073567151	No Hit
GAGTACGGGAAGCAGTGGTATCAAC	1529	0.32698887938408894	No Hit
AAAAAGTACTCTGCGTTGATACCAC	1391	0.2974764756201882	No Hit
GCGTTGATACCACTGCTTCCATGTA	1332	0.2848588537211291	No Hit
GTATCAACGCAGAGTACGGGAAGCA	1294	0.276732249786142	No Hit
GTATCAACGCAGAGTACATGGAAGC	1231	0.26325919589392643	No Hit
GCAGTGGTATCAACGCAGAGTACGG	1157	0.24743370402053036	No Hit
GCAGAGTACGGAAGCAGTGGTATCA	1146	0.24508126603934988	No Hit
GGTATCAACGCAGAGTACATGGGAA	1144	0.2446535500427716	No Hit
GCTTCCCGTACTCTGCGTTGATACC	1130	0.2416595380667237	No Hit
CCATGTACTCTGCGTTGATACCACT	1118	0.23909324208725405	No Hit
CACTGCTTCCGTACTCTGCGTTGAT	1071	0.22904191616766467	No Hit
TGATACCACTGCTTCCGTACTCTGC	1008	0.2155688622754491	No Hit
GCGTTGATACCACTGCTTCCCGTAC	1002	0.2142857142857143	No Hit
TATCAACGCAGAGTACATGGAAGCA	984	0.21043627031650985	No Hit
CCCCATGTACTCTGCGTTGATACCA	913	0.19525235243798117	No Hit
AAAGTACTCTGCGTTGATACCACTG	912	0.19503849443969204	No Hit
GGGAAGCAGTGGTATCAACGCAGAG	904	0.19332763045337895	No Hit
GCCCATGTACTCTGCGTTGATACCA	898	0.19204448246364414	No Hit
ATACCACTGCTTCCCATGTACTCTG	873	0.18669803250641573	No Hit
CTGCGTTGATACCACTGCTTCCGTA	873	0.18669803250641573	No Hit
TACCACTGCTTCCGTACTCTGCGTT	870	0.18605645851154834	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	854	0.18263473053892215	No Hit
CTGCTTCCCATGTACTCTGCGTTGA	850	0.1817792985457656	No Hit
TATCAACGCAGAGTACGGGAAGCAG	793	0.16958939264328485	No Hit
TACGGAAGCAGTGGTATCAACGCAG	772	0.165098374679213	No Hit
TCCCATGTACTCTGCGTTGATACCA	769	0.1644568006843456	No Hit
GCAGTGGTATCAACGCAGAGTACAT	750	0.16039349871685202	No Hit
CATGGGAAGCAGTGGTATCAACGCA	734	0.15697177074422583	No Hit
GTGGTATCAACGCAGAGTACATGGG	722	0.1544054747647562	No Hit
ACGGAAGCAGTGGTATCAACGCAGA	706	0.15098374679213003	No Hit
TCAACGCAGAGTACGGAAGCAGTGG	696	0.14884516680923868	No Hit
GTATCAACGCAGAGTACTTTTTTTT	670	0.14328485885372114	No Hit
TACCACTGCTTCCCATGTACTCTGC	665	0.14221556886227546	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	663	0.14178785286569717	No Hit
CTCTGCGTTGATACCACTGCTTCCG	648	0.13857998289136014	No Hit
CTTCCCATGTACTCTGCGTTGATAC	629	0.13451668092386654	No Hit
ATACCACTGCTTCCATGTACTCTGC	620	0.13259195893926431	No Hit
GCAGAGTACATGGGAAGCAGTGGTA	559	0.11954662104362702	No Hit
GGTATCAACGCAGAGTACATGGAAG	555	0.11869118905047048	No Hit
AGCAGTGGTATCAACGCAGAGTACG	540	0.11548331907613345	No Hit
CCACTGCTTCCCATGTACTCTGCGT	518	0.11077844311377245	No Hit
GATACCACTGCTTCCCATGTACTCT	517	0.11056458511548332	No Hit
GGTATCAACGCAGAGTACGGGAAGC	513	0.10970915312232678	No Hit
GTTGATACCACTGCTTCCCATGTAC	502	0.1073567151411463	No Hit
ACATGGGAAGCAGTGGTATCAACGC	493	0.10543199315654406	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.13857998289136E-4	0.0
10	0.0	0.0	0.0	4.27715996578272E-4	0.0
11	0.0	0.0	0.0	0.0014970059880239522	0.0
12	0.0	0.0	0.0	0.0025662959794696323	0.0
13	0.0	0.0	0.0	0.003421727972626176	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTGCAG	25	0.005972796	19.029108	8
TACTTGG	35	0.0022394871	16.20584	2
TCTTCGA	45	6.699121E-4	14.792444	12
GGGCATT	45	6.7045976E-4	14.790851	19
GGGACGG	40	0.0052184495	14.271831	8
TTAACTG	40	0.0052184495	14.271831	5
TACACAG	40	0.0052184495	14.271831	5
CGAACCT	40	0.0052408916	14.262605	16
CGGGGCA	40	0.0054465486	14.180109	4
TGTAGAA	40	0.0054465486	14.180109	2
GTACAAA	95	5.0840754E-9	13.956749	1
GAGTATG	55	2.0075175E-4	13.775493	1
CCACCCG	55	2.0075175E-4	13.775493	1
AAAAAGT	245	0.0	13.529502	1
AAAAGTA	255	0.0	13.345985	2
TGCCCGT	50	0.001553295	13.234768	2
GCCCATG	190	0.0	12.959838	1
AGTATGG	60	4.255354E-4	12.604542	2
ACACTGT	85	3.8598664E-6	12.312952	6
CGTCTTC	55	0.0030279532	12.109433	10
>>END_MODULE