FastQCFastQC Report
Thu 2 Feb 2017
SRR4050280_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050280_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467600
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA310186.63344739093242No Hit
CTTCCGTACTCTGCGTTGATACCAC204594.375320786997434No Hit
GAGTACGGAAGCAGTGGTATCAACG180003.849443969204448No Hit
TCCGTACTCTGCGTTGATACCACTG176273.7696749358426005No Hit
GCTTCCGTACTCTGCGTTGATACCA151203.2335329341317367No Hit
CCCATGTACTCTGCGTTGATACCAC106882.2857142857142856No Hit
GTACATGGGAAGCAGTGGTATCAAC89711.918520102651839No Hit
GCGTTGATACCACTGCTTCCGTACT81021.7326775021385799No Hit
GTATCAACGCAGAGTACGGAAGCAG75901.6231822070145423No Hit
TCCATGTACTCTGCGTTGATACCAC70671.5113344739093242No Hit
GTACATGGAAGCAGTGGTATCAACG67841.4508126603934988No Hit
TATCAACGCAGAGTACGGAAGCAGT65311.3967065868263473No Hit
ACTCTGCGTTGATACCACTGCTTCC58551.2521385799828915No Hit
CAGTGGTATCAACGCAGAGTACGGA44140.9439692044482464No Hit
ACGCAGAGTACGGAAGCAGTGGTAT42910.9176646706586825No Hit
CATGTACTCTGCGTTGATACCACTG41330.8838751069289991No Hit
GGTATCAACGCAGAGTACGGAAGCA40360.8631308810949528No Hit
ATACCACTGCTTCCGTACTCTGCGT36150.7730966638152267No Hit
GAGTACATGGGAAGCAGTGGTATCA33240.7108639863130881No Hit
AAGCAGTGGTATCAACGCAGAGTAC33150.7089392643284859No Hit
GTGGTATCAACGCAGAGTACGGAAG29480.630453378956373No Hit
GAAGCAGTGGTATCAACGCAGAGTA28830.6165526090675791No Hit
GTACGGGAAGCAGTGGTATCAACGC27750.5934559452523525No Hit
GCGTTGATACCACTGCTTCCCATGT24800.5303678357570573No Hit
GTATCAACGCAGAGTACATGGGAAG24440.5226689478186484No Hit
GAGTACATGGAAGCAGTGGTATCAA24200.5175363558597091No Hit
ATCAACGCAGAGTACGGAAGCAGTG22850.4886655260906758No Hit
TTCCCGTACTCTGCGTTGATACCAC21140.45209580838323354No Hit
GCTTCCCATGTACTCTGCGTTGATA21140.45209580838323354No Hit
GATACCACTGCTTCCGTACTCTGCG21000.4491017964071856No Hit
CCCGTACTCTGCGTTGATACCACTG20180.4315654405474764No Hit
TATCAACGCAGAGTACATGGGAAGC19850.424508126603935No Hit
CGGAAGCAGTGGTATCAACGCAGAG19800.42343883661248927No Hit
CCACTGCTTCCGTACTCTGCGTTGA19660.42044482463644134No Hit
GTACTCTGCGTTGATACCACTGCTT19360.41402908468776733No Hit
GTTGATACCACTGCTTCCGTACTCT18180.38879384088964924No Hit
CATGGAAGCAGTGGTATCAACGCAG17330.3706159110350727No Hit
ACGCAGAGTACATGGGAAGCAGTGG17260.36911890504704875No Hit
GCTTCCATGTACTCTGCGTTGATAC16430.35136869118905045No Hit
GAGTACGGGAAGCAGTGGTATCAAC16040.34302822925577414No Hit
CAGTGGTATCAACGCAGAGTACATG16020.3426005132591959No Hit
CTGCTTCCGTACTCTGCGTTGATAC16000.3421727972626176No Hit
AAAAAGTACTCTGCGTTGATACCAC15570.3329769033361848No Hit
CTTCCCGTACTCTGCGTTGATACCA14400.30795551753635586No Hit
GCGTTGATACCACTGCTTCCATGTA14190.303464499572284No Hit
GCAGTGGTATCAACGCAGAGTACGG13670.2923438836612489No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13450.2876390076988879No Hit
GCAGAGTACGGAAGCAGTGGTATCA12980.27758768177929855No Hit
GTATCAACGCAGAGTACGGGAAGCA12560.2686056458511548No Hit
GTATCAACGCAGAGTACATGGAAGC11820.25278015397775877No Hit
CACTGCTTCCGTACTCTGCGTTGAT11560.24721984602224123No Hit
CCATGTACTCTGCGTTGATACCACT11520.24636441402908468No Hit
GTATCAACGCAGAGTACTTTTTTTT11070.23674080410607357No Hit
GGTATCAACGCAGAGTACATGGGAA10910.2333190761334474No Hit
GCCCATGTACTCTGCGTTGATACCA10550.22562018819503848No Hit
CCCCATGTACTCTGCGTTGATACCA10400.22241231822070143No Hit
AAAGTACTCTGCGTTGATACCACTG10390.22219846022241233No Hit
GCTTCCCGTACTCTGCGTTGATACC10210.21834901625320788No Hit
TATCAACGCAGAGTACATGGAAGCA10190.2179213002566296No Hit
TGATACCACTGCTTCCGTACTCTGC10000.21385799828913601No Hit
GGGAAGCAGTGGTATCAACGCAGAG9740.20829769033361847No Hit
GCGTTGATACCACTGCTTCCCGTAC9740.20829769033361847No Hit
TACCACTGCTTCCGTACTCTGCGTT9550.2042343883661249No Hit
ATACCACTGCTTCCCATGTACTCTG9280.1984602224123182No Hit
CTGCGTTGATACCACTGCTTCCGTA9240.19760479041916168No Hit
TATCAACGCAGAGTACGGGAAGCAG9060.19375534644995723No Hit
ACGCAGAGTACATGGAAGCAGTGGT9050.1935414884516681No Hit
CATGGGAAGCAGTGGTATCAACGCA8820.18862275449101795No Hit
TATCAACGCAGAGTACTTTTTTTTT8690.18584260051325918No Hit
TACGGAAGCAGTGGTATCAACGCAG8620.18434559452523525No Hit
CTGCTTCCCATGTACTCTGCGTTGA8320.17792985457656116No Hit
TCCCATGTACTCTGCGTTGATACCA8270.17686056458511548No Hit
ACGGAAGCAGTGGTATCAACGCAGA8120.17365269461077845No Hit
TCAACGCAGAGTACGGAAGCAGTGG8020.17151411462788707No Hit
GTGGTATCAACGCAGAGTACATGGG7890.16873396065012833No Hit
GCAGTGGTATCAACGCAGAGTACAT7840.16766467065868262No Hit
TACCACTGCTTCCCATGTACTCTGC7300.1561163387510693No Hit
GCAGAGTACATGGGAAGCAGTGGTA6810.14563729683490162No Hit
CTTCCCATGTACTCTGCGTTGATAC6520.1394354148845167No Hit
CTCTGCGTTGATACCACTGCTTCCG6240.13344739093242086No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6080.1300256629597947No Hit
ATACCACTGCTTCCATGTACTCTGC6080.1300256629597947No Hit
AGCAGTGGTATCAACGCAGAGTACG6040.12917023096663816No Hit
CCACTGCTTCCCATGTACTCTGCGT5750.12296834901625321No Hit
ACATGGGAAGCAGTGGTATCAACGC5610.11997433704020531No Hit
GATACCACTGCTTCCCATGTACTCT5430.11612489307100085No Hit
GGTATCAACGCAGAGTACATGGAAG5290.11313088109495295No Hit
GGTATCAACGCAGAGTACGGGAAGC5250.1122754491017964No Hit
GTTGATACCACTGCTTCCCATGTAC5190.1109923011120616No Hit
ATCAACGCAGAGTACATGGGAAGCA5080.10863986313088109No Hit
ACGCAGAGTACTTTTTTTTTTTTTT5020.1073567151411463No Hit
GGTATCAACGCAGAGTACTTTTTTT4910.10500427715996578No Hit
AACGCAGAGTACGGAAGCAGTGGTA4810.10286569717707442No Hit
CAACGCAGAGTACGGAAGCAGTGGT4740.10136869118905047No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGT250.00588220819.0795361
GGACGGA601.3991466E-615.8996131
GACGGAA601.4324396E-615.8638072
AAAAAGT3100.015.0789871
AAAAGTA3250.014.3506442
ATAGGAC400.005204950514.2774263
GACCGCG400.00529449814.2407747
CCGCGGT701.0954459E-412.2050739
GGACCGC550.003077822712.083086
CTTCTAT855.0830786E-511.2232561
AGGACCG600.005884208311.0785275
GGGGGCA600.00590768411.07260518
ACCGCGG803.7863659E-410.6794398
AAGTACT4450.010.4808074
AAAGTAC4500.010.1528373
AGTACTC4600.010.1151785
TTCTATA951.6067448E-410.0192472
TCCATGT13400.09.6821531
TCTATAG800.0044345479.5182843
GCCCATG1606.2959225E-78.9435321
Produced by FastQC (version 0.11.5)