##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4050280_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 467600 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.56991017964072 31.0 31.0 34.0 27.0 34.0 2 30.387399486740804 31.0 30.0 34.0 26.0 34.0 3 30.76303892215569 31.0 31.0 34.0 27.0 34.0 4 34.28044910179641 37.0 35.0 37.0 30.0 37.0 5 34.17459153122327 37.0 35.0 37.0 30.0 37.0 6 34.206800684345595 37.0 35.0 37.0 30.0 37.0 7 34.08170230966638 37.0 35.0 37.0 30.0 37.0 8 34.10461505560308 37.0 35.0 37.0 30.0 37.0 9 35.66839178785287 39.0 37.0 39.0 30.0 39.0 10 35.62602651839179 39.0 37.0 39.0 30.0 39.0 11 35.628999144568006 39.0 37.0 39.0 30.0 39.0 12 35.59759195893926 39.0 37.0 39.0 30.0 39.0 13 35.458954234388365 39.0 35.0 39.0 30.0 39.0 14 36.63153336184773 40.0 37.0 41.0 30.0 41.0 15 36.56133019674936 40.0 37.0 41.0 30.0 41.0 16 36.36098160821215 39.0 36.0 41.0 30.0 41.0 17 36.41132164242943 40.0 37.0 41.0 30.0 41.0 18 36.2872754491018 39.0 36.0 41.0 30.0 41.0 19 36.411610350727116 40.0 37.0 41.0 30.0 41.0 20 36.4237254063302 40.0 37.0 41.0 30.0 41.0 21 36.39417664670659 40.0 37.0 41.0 30.0 41.0 22 36.38061804961506 40.0 37.0 41.0 30.0 41.0 23 36.27940119760479 40.0 37.0 41.0 30.0 41.0 24 36.15465141146279 40.0 37.0 41.0 30.0 41.0 25 35.902983319076135 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5166076558514554 1101 2 0.9697341427743602 1101 3 0.6815386428277996 1101 4 0.6216152976052598 1101 5 0.9356735621113472 1101 6 0.531269560994545 1101 7 0.8619085122216106 1101 8 0.8948014411264822 1101 9 0.9169751466844431 1101 10 0.8590859429126567 1101 11 0.7963960944910156 1101 12 0.9409870315415887 1101 13 1.013445529169779 1101 14 1.098006928902123 1101 15 1.0956806689040377 1101 16 0.9792587199662748 1101 17 1.037328357918767 1101 18 1.0053954136578866 1101 19 1.0276789730005262 1101 20 0.8774941888843415 1101 21 0.6416472836388465 1101 22 0.9594352601718796 1101 23 1.2515287666229895 1101 24 1.1681950621032016 1101 25 1.220638707185202 1102 1 0.6253345846951 1102 2 0.8649192576098166 1102 3 0.6397509035283306 1102 4 0.39928350942140867 1102 5 0.3827983312482317 1102 6 0.5794204333237047 1102 7 0.5250079985638081 1102 8 0.6091777975104264 1102 9 0.6014716529222923 1102 10 0.6269093836520625 1102 11 0.5302457798705547 1102 12 0.3515333840054069 1102 13 0.6365253744662382 1102 14 0.5598867305518027 1102 15 0.7327557696351263 1102 16 1.0182316468458126 1102 17 0.9261503397245363 1102 18 1.0925454817549323 1102 19 0.8573393768947923 1102 20 0.8239763805987295 1102 21 0.7162448644762947 1102 22 0.6417191177379209 1102 23 0.6057121881254872 1102 24 0.7079338281120684 1102 25 0.7342540740807451 1103 1 0.05176161537665891 1103 2 0.49111851069245915 1103 3 0.4623741287947425 1103 4 0.21374537478311595 1103 5 0.317660376900335 1103 6 0.07227723123748575 1103 7 0.19419524301827096 1103 8 -0.17349581248281254 1103 9 0.15812227589000116 1103 10 -0.2477606205332492 1103 11 -0.006806311176092095 1103 12 0.12041960788744888 1103 13 0.14116141322867293 1103 14 0.3483674620157018 1103 15 0.2760898541784442 1103 16 0.22279685723827214 1103 17 0.34501563704271376 1103 18 0.34036831424583625 1103 19 0.48435557083574565 1103 20 0.40074065090554 1103 21 0.53807127058041 1103 22 0.5330176915121072 1103 23 0.5589010745103451 1103 24 0.46949575464373083 1103 25 0.517177193104601 1104 1 0.6867017109753952 1104 2 1.1011268911009289 1104 3 0.8815031870851406 1104 4 0.8854040748886476 1104 5 0.7628462978141073 1104 6 0.710810038984711 1104 7 0.6739813874096043 1104 8 0.6031740615006811 1104 9 0.6676433510653723 1104 10 0.5781829513830701 1104 11 0.7102074699400518 1104 12 0.8467604732422203 1104 13 0.6193413126637708 1104 14 0.9478460143777312 1104 15 0.7369192042312065 1104 16 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2215 2 -1.9037604867146278 2215 3 -1.1243990360489384 2215 4 -0.8121390468781371 2215 5 -0.6510383981719343 2215 6 -0.4435820063865705 2215 7 -0.6741362027540916 2215 8 -0.7436795516417547 2215 9 -1.1407816969291176 2215 10 -0.6583163085492245 2215 11 -0.8882215348520646 2215 12 -0.7071032663711918 2215 13 -0.7823029565743269 2215 14 -0.9380003739453144 2215 15 -0.8356587226999324 2215 16 -1.2860101048813632 2215 17 -0.9187722507765557 2215 18 -1.0217788857064747 2215 19 -1.0302705430735912 2215 20 -1.1499962429638586 2215 21 -0.789641601591967 2215 22 -0.787434279995658 2215 23 -1.044577004946042 2215 24 -1.0070152875879614 2215 25 -1.2474899007469205 2216 1 -2.8735192834997427 2216 2 -3.97773757345767 2216 3 -2.732796801159836 2216 4 -2.9093002862257293 2216 5 -3.0983209761955273 2216 6 -3.050427765176572 2216 7 -3.059061988633651 2216 8 -2.9832077417202 2216 9 -3.021694841011744 2216 10 -3.2332076681067576 2216 11 -2.7969748505019325 2216 12 -3.194958111452742 2216 13 -3.218580398867907 2216 14 -3.459688956822454 2216 15 -3.3630925507940646 2216 16 -3.912512729182957 2216 17 -3.575812715814088 2216 18 -4.033376605668103 2216 19 -3.899089325171907 2216 20 -3.910310030423645 2216 21 -3.8971307375477267 2216 22 -3.943667701746321 2216 23 -3.793812289104288 2216 24 -4.225944121043579 2216 25 -4.032099641702953 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8639.0 3 1.0 4 140.0 5 1225.0 6 1795.0 7 1227.0 8 908.0 9 631.0 10 663.0 11 670.0 12 532.0 13 530.0 14 532.0 15 523.0 16 451.0 17 550.0 18 674.0 19 933.0 20 1163.0 21 1466.0 22 1786.0 23 2164.0 24 2468.0 25 2788.0 26 3346.0 27 3787.0 28 4175.0 29 5076.0 30 6578.0 31 8590.0 32 11461.0 33 15696.0 34 21456.0 35 30782.0 36 47465.0 37 88420.0 38 188309.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.953640568198125 13.909543303578124 14.804770259356612 22.33204586886714 2 8.526587974617215 22.757030633436067 37.95397819749722 30.762403194449494 3 22.643498716852008 30.320573139435414 27.010692899914456 20.025235243798118 4 14.393884221754794 14.699758813172314 30.548689323091814 40.357667641981074 5 17.15324953196042 32.009414281893555 28.835089596148705 22.002246589997327 6 38.76905168964389 27.566240930186776 12.50077565492022 21.163931725249117 7 25.14660294083039 32.04833350805166 22.365158455261767 20.439905095856183 8 25.805789189975442 34.25128130534854 25.565143360742006 14.377786143934014 9 27.73833790712249 23.11307134041611 14.142312932736424 35.00627781972497 10 12.940620381519876 25.593571610369775 30.329097788262672 31.136710219847675 11 29.588879358839172 26.429703525144006 16.668769932003926 27.3126471840129 12 26.37121009571681 23.413293406769693 24.78220416020534 25.433292337308167 13 33.837003387742534 18.75320808951853 27.522627724737365 19.887160798001574 14 33.97930965157487 18.537046503023134 22.76430241187781 24.719341433524182 15 37.098826553634865 15.917766794911003 26.967238883055277 20.01616776839886 16 29.091317365269457 18.977758768177928 33.792771599657826 18.13815226689478 17 24.99700598802395 28.983319076133444 29.860778443113773 16.15889649272883 18 28.023738237810093 15.97818648417451 40.783789563729684 15.214285714285714 19 24.075278015397775 36.703378956372966 18.12938408896493 21.09195893926433 20 17.530581693755344 29.834901625320786 31.063515825491873 21.571000855431993 21 18.64409679523509 35.38586567148219 21.040238673182415 24.929798860100302 22 17.66604288082126 32.99256803721328 16.240389242367534 33.100999839597925 23 22.801802399064155 24.669856009101814 17.462826053947932 35.06551553788609 24 16.419407476159822 30.572527817995564 18.978684454215525 34.029380251629085 25 27.413197678277417 28.558643620722506 17.701743004750924 26.326415696249146 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 315.0 1 315.0 2 403.5 3 492.0 4 492.0 5 492.0 6 1086.0 7 1680.0 8 1680.0 9 1680.0 10 1463.5 11 1247.0 12 1247.0 13 1247.0 14 1097.5 15 948.0 16 948.0 17 948.0 18 1412.0 19 1876.0 20 1876.0 21 1876.0 22 2689.5 23 3503.0 24 3503.0 25 3503.0 26 4906.5 27 6310.0 28 6310.0 29 6310.0 30 7447.0 31 8584.0 32 8584.0 33 8584.0 34 9560.0 35 10536.0 36 10536.0 37 10536.0 38 11885.0 39 13234.0 40 13234.0 41 13234.0 42 17822.0 43 22410.0 44 22410.0 45 22410.0 46 61323.0 47 100236.0 48 100236.0 49 100236.0 50 158445.5 51 216655.0 52 216655.0 53 216655.0 54 131408.5 55 46162.0 56 46162.0 57 46162.0 58 29388.0 59 12614.0 60 12614.0 61 12614.0 62 10406.5 63 8199.0 64 8199.0 65 8199.0 66 6749.5 67 5300.0 68 5300.0 69 5300.0 70 4148.0 71 2996.0 72 2996.0 73 2996.0 74 2446.0 75 1896.0 76 1896.0 77 1896.0 78 1637.0 79 1378.0 80 1378.0 81 1378.0 82 983.5 83 589.0 84 589.0 85 589.0 86 445.0 87 301.0 88 301.0 89 301.0 90 216.0 91 131.0 92 131.0 93 131.0 94 68.5 95 6.0 96 6.0 97 6.0 98 4.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1691616766467066 2 0.008340461933276304 3 0.0 4 0.15868263473053892 5 0.0481180496150556 6 0.054106073567151414 7 0.03870829769033362 8 0.02309666381522669 9 0.017322497861420018 10 0.010479041916167664 11 0.015611633875106928 12 0.0160393498716852 13 0.006843455945252352 14 0.008340461933276304 15 6.415739948674081E-4 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0032078699743370406 22 0.0053464499572284 23 2.13857998289136E-4 24 0.0014970059880239522 25 0.11398631308810948 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 467600.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.653142805258284 #Duplication Level Percentage of deduplicated Percentage of total 1 88.6245545801574 19.190001363765553 2 5.646208531376614 2.4451631927633097 3 1.5849972551880804 1.0296051573758973 4 0.7958606356017679 0.6893153598307482 5 0.5359008751458999 0.5801969089498531 6 0.3909274716109341 0.5078885021574067 7 0.30994248680171393 0.4697860249694076 8 0.22195881272708962 0.38448846950922017 9 0.186965306196819 0.36435478272377075 >10 1.3497495367066883 5.820150908309746 >50 0.14497309838700853 2.1748711510518692 >100 0.11697829318124135 5.412281383266647 >500 0.031994063092305326 5.1511929293378635 >1k 0.04599146569518891 20.31683834776167 >5k 0.00699870130144179 11.019404896330894 >10k+ 0.00599888682980725 24.444460621896148 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACGGAAGCAGTGGTATCAACGCA 31018 6.63344739093242 No Hit CTTCCGTACTCTGCGTTGATACCAC 20459 4.375320786997434 No Hit GAGTACGGAAGCAGTGGTATCAACG 18000 3.849443969204448 No Hit TCCGTACTCTGCGTTGATACCACTG 17627 3.7696749358426005 No Hit GCTTCCGTACTCTGCGTTGATACCA 15120 3.2335329341317367 No Hit CCCATGTACTCTGCGTTGATACCAC 10688 2.2857142857142856 No Hit GTACATGGGAAGCAGTGGTATCAAC 8971 1.918520102651839 No Hit GCGTTGATACCACTGCTTCCGTACT 8102 1.7326775021385799 No Hit GTATCAACGCAGAGTACGGAAGCAG 7590 1.6231822070145423 No Hit TCCATGTACTCTGCGTTGATACCAC 7067 1.5113344739093242 No Hit GTACATGGAAGCAGTGGTATCAACG 6784 1.4508126603934988 No Hit TATCAACGCAGAGTACGGAAGCAGT 6531 1.3967065868263473 No Hit ACTCTGCGTTGATACCACTGCTTCC 5855 1.2521385799828915 No Hit CAGTGGTATCAACGCAGAGTACGGA 4414 0.9439692044482464 No Hit ACGCAGAGTACGGAAGCAGTGGTAT 4291 0.9176646706586825 No Hit CATGTACTCTGCGTTGATACCACTG 4133 0.8838751069289991 No Hit GGTATCAACGCAGAGTACGGAAGCA 4036 0.8631308810949528 No Hit ATACCACTGCTTCCGTACTCTGCGT 3615 0.7730966638152267 No Hit GAGTACATGGGAAGCAGTGGTATCA 3324 0.7108639863130881 No Hit AAGCAGTGGTATCAACGCAGAGTAC 3315 0.7089392643284859 No Hit GTGGTATCAACGCAGAGTACGGAAG 2948 0.630453378956373 No Hit GAAGCAGTGGTATCAACGCAGAGTA 2883 0.6165526090675791 No Hit GTACGGGAAGCAGTGGTATCAACGC 2775 0.5934559452523525 No Hit GCGTTGATACCACTGCTTCCCATGT 2480 0.5303678357570573 No Hit GTATCAACGCAGAGTACATGGGAAG 2444 0.5226689478186484 No Hit GAGTACATGGAAGCAGTGGTATCAA 2420 0.5175363558597091 No Hit ATCAACGCAGAGTACGGAAGCAGTG 2285 0.4886655260906758 No Hit TTCCCGTACTCTGCGTTGATACCAC 2114 0.45209580838323354 No Hit GCTTCCCATGTACTCTGCGTTGATA 2114 0.45209580838323354 No Hit GATACCACTGCTTCCGTACTCTGCG 2100 0.4491017964071856 No Hit CCCGTACTCTGCGTTGATACCACTG 2018 0.4315654405474764 No Hit TATCAACGCAGAGTACATGGGAAGC 1985 0.424508126603935 No Hit CGGAAGCAGTGGTATCAACGCAGAG 1980 0.42343883661248927 No Hit CCACTGCTTCCGTACTCTGCGTTGA 1966 0.42044482463644134 No Hit GTACTCTGCGTTGATACCACTGCTT 1936 0.41402908468776733 No Hit GTTGATACCACTGCTTCCGTACTCT 1818 0.38879384088964924 No Hit CATGGAAGCAGTGGTATCAACGCAG 1733 0.3706159110350727 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1726 0.36911890504704875 No Hit GCTTCCATGTACTCTGCGTTGATAC 1643 0.35136869118905045 No Hit GAGTACGGGAAGCAGTGGTATCAAC 1604 0.34302822925577414 No Hit CAGTGGTATCAACGCAGAGTACATG 1602 0.3426005132591959 No Hit CTGCTTCCGTACTCTGCGTTGATAC 1600 0.3421727972626176 No Hit AAAAAGTACTCTGCGTTGATACCAC 1557 0.3329769033361848 No Hit CTTCCCGTACTCTGCGTTGATACCA 1440 0.30795551753635586 No Hit GCGTTGATACCACTGCTTCCATGTA 1419 0.303464499572284 No Hit GCAGTGGTATCAACGCAGAGTACGG 1367 0.2923438836612489 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1345 0.2876390076988879 No Hit GCAGAGTACGGAAGCAGTGGTATCA 1298 0.27758768177929855 No Hit GTATCAACGCAGAGTACGGGAAGCA 1256 0.2686056458511548 No Hit GTATCAACGCAGAGTACATGGAAGC 1182 0.25278015397775877 No Hit CACTGCTTCCGTACTCTGCGTTGAT 1156 0.24721984602224123 No Hit CCATGTACTCTGCGTTGATACCACT 1152 0.24636441402908468 No Hit GTATCAACGCAGAGTACTTTTTTTT 1107 0.23674080410607357 No Hit GGTATCAACGCAGAGTACATGGGAA 1091 0.2333190761334474 No Hit GCCCATGTACTCTGCGTTGATACCA 1055 0.22562018819503848 No Hit CCCCATGTACTCTGCGTTGATACCA 1040 0.22241231822070143 No Hit AAAGTACTCTGCGTTGATACCACTG 1039 0.22219846022241233 No Hit GCTTCCCGTACTCTGCGTTGATACC 1021 0.21834901625320788 No Hit TATCAACGCAGAGTACATGGAAGCA 1019 0.2179213002566296 No Hit TGATACCACTGCTTCCGTACTCTGC 1000 0.21385799828913601 No Hit GGGAAGCAGTGGTATCAACGCAGAG 974 0.20829769033361847 No Hit GCGTTGATACCACTGCTTCCCGTAC 974 0.20829769033361847 No Hit TACCACTGCTTCCGTACTCTGCGTT 955 0.2042343883661249 No Hit ATACCACTGCTTCCCATGTACTCTG 928 0.1984602224123182 No Hit CTGCGTTGATACCACTGCTTCCGTA 924 0.19760479041916168 No Hit TATCAACGCAGAGTACGGGAAGCAG 906 0.19375534644995723 No Hit ACGCAGAGTACATGGAAGCAGTGGT 905 0.1935414884516681 No Hit CATGGGAAGCAGTGGTATCAACGCA 882 0.18862275449101795 No Hit TATCAACGCAGAGTACTTTTTTTTT 869 0.18584260051325918 No Hit TACGGAAGCAGTGGTATCAACGCAG 862 0.18434559452523525 No Hit CTGCTTCCCATGTACTCTGCGTTGA 832 0.17792985457656116 No Hit TCCCATGTACTCTGCGTTGATACCA 827 0.17686056458511548 No Hit ACGGAAGCAGTGGTATCAACGCAGA 812 0.17365269461077845 No Hit TCAACGCAGAGTACGGAAGCAGTGG 802 0.17151411462788707 No Hit GTGGTATCAACGCAGAGTACATGGG 789 0.16873396065012833 No Hit GCAGTGGTATCAACGCAGAGTACAT 784 0.16766467065868262 No Hit TACCACTGCTTCCCATGTACTCTGC 730 0.1561163387510693 No Hit GCAGAGTACATGGGAAGCAGTGGTA 681 0.14563729683490162 No Hit CTTCCCATGTACTCTGCGTTGATAC 652 0.1394354148845167 No Hit CTCTGCGTTGATACCACTGCTTCCG 624 0.13344739093242086 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 608 0.1300256629597947 No Hit ATACCACTGCTTCCATGTACTCTGC 608 0.1300256629597947 No Hit AGCAGTGGTATCAACGCAGAGTACG 604 0.12917023096663816 No Hit CCACTGCTTCCCATGTACTCTGCGT 575 0.12296834901625321 No Hit ACATGGGAAGCAGTGGTATCAACGC 561 0.11997433704020531 No Hit GATACCACTGCTTCCCATGTACTCT 543 0.11612489307100085 No Hit GGTATCAACGCAGAGTACATGGAAG 529 0.11313088109495295 No Hit GGTATCAACGCAGAGTACGGGAAGC 525 0.1122754491017964 No Hit GTTGATACCACTGCTTCCCATGTAC 519 0.1109923011120616 No Hit ATCAACGCAGAGTACATGGGAAGCA 508 0.10863986313088109 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 502 0.1073567151411463 No Hit GGTATCAACGCAGAGTACTTTTTTT 491 0.10500427715996578 No Hit AACGCAGAGTACGGAAGCAGTGGTA 481 0.10286569717707442 No Hit CAACGCAGAGTACGGAAGCAGTGGT 474 0.10136869118905047 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 2.13857998289136E-4 0.0 11 0.0 0.0 0.0 0.00106928999144568 0.0 12 0.0 0.0 0.0 0.00213857998289136 0.0 13 0.0 0.0 0.0 0.002780153977758768 2.13857998289136E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCCGT 25 0.005882208 19.079536 1 GGACGGA 60 1.3991466E-6 15.899613 1 GACGGAA 60 1.4324396E-6 15.863807 2 AAAAAGT 310 0.0 15.078987 1 AAAAGTA 325 0.0 14.350644 2 ATAGGAC 40 0.0052049505 14.277426 3 GACCGCG 40 0.005294498 14.240774 7 CCGCGGT 70 1.0954459E-4 12.205073 9 GGACCGC 55 0.0030778227 12.08308 6 CTTCTAT 85 5.0830786E-5 11.223256 1 AGGACCG 60 0.0058842083 11.078527 5 GGGGGCA 60 0.005907684 11.072605 18 ACCGCGG 80 3.7863659E-4 10.679439 8 AAGTACT 445 0.0 10.480807 4 AAAGTAC 450 0.0 10.152837 3 AGTACTC 460 0.0 10.115178 5 TTCTATA 95 1.6067448E-4 10.019247 2 TCCATGT 1340 0.0 9.682153 1 TCTATAG 80 0.004434547 9.518284 3 GCCCATG 160 6.2959225E-7 8.943532 1 >>END_MODULE