FastQCFastQC Report
Thu 2 Feb 2017
SRR4050279_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050279_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5311136
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA114200.21501991287739575No Hit
GTATCTGATCGTCTTCGAACCTCCG70450.13264582191079272No Hit
GAATAGGACCGCGGTTCTATTTTGT69460.13078181390949128No Hit
GTCCTATTCCATTATTCCTAGCTGC65140.1226479608129033No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC61470.1157379513535334No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG61210.11524841389864618No Hit
GATTAAGAGGGACGGCCGGGGGCAT60810.11449527935266579No Hit
GTATCAACGCAGAGTACGGGCGGTG59930.11283838335150898No Hit
CTATTGGAGCTGGAATTACCGCGGC59400.111840480078085No Hit
GACTTAATCAACGCAAGCTTATGAC57930.10907271062160712No Hit
GGGTAGGCACACGCTGAGCCAGTCA56390.10617314261958272No Hit
GTCTTGCGCCGGTCCAAGAATTTCA56380.1061543142559332No Hit
CTTATGACCCGCACTTACTGGGAAT55960.1053635229826538No Hit
GAACTACGACGGTATCTGATCGTCT55890.10523172443710722No Hit
ATACAGGACTCTTTCGAGGCCCTGT55780.10502461243696265No Hit
CTCTTAATCATGGCCTCAGTTCCGA54740.10306646261741367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29250.015.8533781
GTATCAA100250.013.4140931
CGTCGTA9900.012.00916810
ACGCTTA2100.011.77238617
CGAACGA5350.011.55299916
ACCGTCC3150.011.4739038
ACGGTAT10800.011.3606749
ATACCGT11350.011.2287296
TACCGTC11100.011.224477
GCGTTAT4050.011.2159951
CGGTTCT13350.011.04062912
GTCGTAG11300.010.9421611
GTCCTAT14250.010.8913211
GGTTCTA13850.010.77967413
GCTCGTA5400.010.7442039
GCGCGCT5150.010.70917315
CCGTCGT12000.010.700179
AACCGCG5550.010.6246897
CTCGACG2350.010.52110213
ACTCTAA11550.010.4582710
Produced by FastQC (version 0.11.5)