##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050279_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5311136
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.994044965144933	31.0	31.0	34.0	26.0	34.0
2	30.104155871738175	31.0	31.0	34.0	26.0	34.0
3	30.231813306983668	31.0	31.0	34.0	26.0	34.0
4	33.75107641754984	37.0	35.0	37.0	30.0	37.0
5	33.739850947142	37.0	35.0	37.0	29.0	37.0
6	33.774364279129735	37.0	35.0	37.0	30.0	37.0
7	33.68935647665584	37.0	35.0	37.0	29.0	37.0
8	33.65369630903822	37.0	35.0	37.0	29.0	37.0
9	35.181246723864724	39.0	35.0	39.0	29.0	39.0
10	35.058147447175145	39.0	35.0	39.0	28.0	39.0
11	34.954983265350386	39.0	35.0	39.0	28.0	39.0
12	34.86730145867099	39.0	35.0	39.0	27.0	39.0
13	34.86779306724588	39.0	35.0	39.0	27.0	39.0
14	35.97950852698933	40.0	36.0	41.0	27.0	41.0
15	35.91997418254776	40.0	36.0	41.0	27.0	41.0
16	35.918469796292165	40.0	36.0	41.0	27.0	41.0
17	35.879835688636106	40.0	36.0	41.0	27.0	41.0
18	35.83345352105463	40.0	36.0	41.0	27.0	41.0
19	35.8490102682364	40.0	37.0	41.0	27.0	41.0
20	35.79772199393877	40.0	37.0	41.0	27.0	41.0
21	35.73910063685057	40.0	36.0	41.0	27.0	41.0
22	35.64392457658776	40.0	36.0	41.0	27.0	41.0
23	35.55513716839486	40.0	36.0	41.0	26.0	41.0
24	35.47806363836287	40.0	36.0	41.0	26.0	41.0
25	35.238216645177225	40.0	36.0	41.0	25.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5629616258259489
1101	2	0.5716986865568217
1101	3	0.5385484281093937
1101	4	0.6244583755572393
1101	5	0.6244335209035441
1101	6	0.5977765037141296
1101	7	0.6153509644088189
1101	8	0.5737038191699995
1101	9	0.6290407273788929
1101	10	0.6123237400899484
1101	11	0.5282222943563255
1101	12	0.5750137530585278
1101	13	0.5515308824273717
1101	14	0.6828496097134291
1101	15	0.7353099135738859
1101	16	0.7867153803369789
1101	17	0.7888548712228243
1101	18	0.7394826024459178
1101	19	0.7837854622737055
1101	20	0.7955967006120659
1101	21	0.7889644659462363
1101	22	0.819298001734289
1101	23	0.8246900264736823
1101	24	0.8764419509960817
1101	25	0.8895747427672518
1102	1	0.5478811186209924
1102	2	0.5596664140769754
1102	3	0.5374249086816505
1102	4	0.5029983541738545
1102	5	0.5200556728632932
1102	6	0.5115610045794838
1102	7	0.47734367941961864
1102	8	0.5385313560972662
1102	9	0.6177563223344364
1102	10	0.6561349141507975
1102	11	0.6156566694223073
1102	12	0.5028031521521825
1102	13	0.5606944721440001
1102	14	0.6414820234571295
1102	15	0.6196752767233349
1102	16	0.7160202048002091
1102	17	0.6368338799127145
1102	18	0.7118949907170418
1102	19	0.7560916580749151
1102	20	0.6784128920968371
1102	21	0.6887591615363888
1102	22	0.7004137979044387
1102	23	0.7233291126958363
1102	24	0.703264695206407
1102	25	0.8310960511377417
1103	1	0.5947125018799682
1103	2	0.5463268932313063
1103	3	0.49118938761834485
1103	4	0.46459704474399643
1103	5	0.5225329956748084
1103	6	0.4281359054181806
1103	7	0.44609550694003275
1103	8	0.5015851246126672
1103	9	0.5527347568502066
1103	10	0.5406016033039265
1103	11	0.44343558351907575
1103	12	0.44066516321598925
1103	13	0.4455268979292697
1103	14	0.6355485321696079
1103	15	0.6172256311961561
1103	16	0.5535965725654748
1103	17	0.42543273881869226
1103	18	0.5743650399687326
1103	19	0.5566890848377284
1103	20	0.6124067472517467
1103	21	0.5471981935036183
1103	22	0.6504506422408198
1103	23	0.6920110684163632
1103	24	0.7280697483862113
1103	25	0.7301942794667369
1104	1	0.5251039775266442
1104	2	0.5600178218260723
1104	3	0.5855144606336147
1104	4	0.5704490023856579
1104	5	0.6264137966689347
1104	6	0.5840131829819555
1104	7	0.5964585300978698
1104	8	0.5995725122335784
1104	9	0.678852156602197
1104	10	0.7062946108369488
1104	11	0.6022106462044619
1104	12	0.5732081789070946
1104	13	0.553373271851946
1104	14	0.6151609694997546
1104	15	0.5237531419916408
1104	16	0.6023559487684409
1104	17	0.6251983254186371
1104	18	0.6859780376255387
1104	19	0.7299462628781157
1104	20	0.7546991864923527
1104	21	0.699304476797245
1104	22	0.7491073056086748
1104	23	0.6644256669825523
1104	24	0.6573857578527367
1104	25	0.7794524206415474
1105	1	0.2809035791109302
1105	2	0.34159062795416517
1105	3	0.41896250384385425
1105	4	0.44309568494487905
1105	5	0.40573745967037667
1105	6	0.3071372919431212
1105	7	0.3788723779017005
1105	8	0.3961832078594085
1105	9	0.46090227435168174
1105	10	0.4034973386836853
1105	11	0.3974998594838155
1105	12	0.3320243741471458
1105	13	0.37790758925473256
1105	14	0.5251931096617426
1105	15	0.42983272257737326
1105	16	0.30807224964900115
1105	17	0.35380246717750197
1105	18	0.46492106785746756
1105	19	0.4291517577695103
1105	20	0.4392000795069535
1105	21	0.4009748730031717
1105	22	0.443220535874147
1105	23	0.4626670213497448
1105	24	0.4654084766225921
1105	25	0.49311590738012967
1106	1	0.29606745504058196
1106	2	0.29827132466115813
1106	3	0.3697006145548798
1106	4	0.37501137192122513
1106	5	0.35404784879598594
1106	6	0.3347787815125045
1106	7	0.3186251692083175
1106	8	0.2745179813433438
1106	9	0.3246494051901223
1106	10	0.4011363107654091
1106	11	0.3345631295234881
1106	12	0.36369499758685464
1106	13	0.30139074526077536
1106	14	0.4247589360516528
1106	15	0.4493258899301438
1106	16	0.3700592329486412
1106	17	0.4962723829981215
1106	18	0.5231967396487178
1106	19	0.5004632655894596
1106	20	0.46670702812185283
1106	21	0.44974630066901256
1106	22	0.4627894095517817
1106	23	0.2935717534622668
1106	24	0.46652815153704097
1106	25	0.5003726830745734
1107	1	0.36380988847102813
1107	2	0.3101097075966983
1107	3	0.24825708439061245
1107	4	0.31814633839063333
1107	5	0.36895684842415477
1107	6	0.23963003140687533
1107	7	0.37890636066119043
1107	8	0.38239841894100834
1107	9	0.447150106589163
1107	10	0.4630293715766456
1107	11	0.4449500252576186
1107	12	0.40047039587320654
1107	13	0.48260507440009803
1107	14	0.47675664123324424
1107	15	0.510974049679092
1107	16	0.43959675135474896
1107	17	0.4963203700791965
1107	18	0.5247443979262485
1107	19	0.5089698211555458
1107	20	0.4975041179386892
1107	21	0.514448724688684
1107	22	0.5414999089282091
1107	23	0.48564922764149543
1107	24	0.5141399830536741
1107	25	0.6090798597416054
1108	1	0.11670573885761826
1108	2	0.08951555326490634
1108	3	0.09330723038910804
1108	4	0.12680577522989012
1108	5	0.06823688062372213
1108	6	0.16192936073335318
1108	7	0.12781570592162694
1108	8	0.0011324303344650843
1108	9	0.010286730127106125
1108	10	-0.03528716100053941
1108	11	1.7469108914980325E-4
1108	12	6.519706670928826E-4
1108	13	-0.019058795909216997
1108	14	-0.12422803834641627
1108	15	0.05334187845458871
1108	16	0.030625720410910162
1108	17	-0.14700839561921697
1108	18	-0.15335153145178992
1108	19	0.032257975604913725
1108	20	0.06240485855882838
1108	21	0.09097922156595928
1108	22	0.07939394281312673
1108	23	0.016974459829604882
1108	24	0.017342980507557115
1108	25	-0.07806918233641369
1109	1	-0.13252785814879786
1109	2	-0.12304845754947635
1109	3	-0.061539772027387585
1109	4	-0.09678901561304087
1109	5	-0.06879671388907127
1109	6	-0.1616645253435891
1109	7	-0.04998396829641649
1109	8	-0.10479952993924257
1109	9	-0.0916330067581157
1109	10	-0.3181359590465149
1109	11	-0.233682104536463
1109	12	-0.13681709764571792
1109	13	-0.2515448434798273
1109	14	-0.20500179421870257
1109	15	-0.16336438827461564
1109	16	-0.13017428360537053
1109	17	-0.18358251244900714
1109	18	-0.12573716323829132
1109	19	-0.1810320547455717
1109	20	-0.2073231224437322
1109	21	-0.25793451307963977
1109	22	-0.1860791278754661
1109	23	-0.16612595694753907
1109	24	-0.18381846869364438
1109	25	-0.21542030005893764
1110	1	0.0049658642664738295
1110	2	-0.021580112330397583
1110	3	0.021019378369157238
1110	4	0.01004310622292337
1110	5	0.06254512618232155
1110	6	0.09234177237872387
1110	7	0.06671392761335682
1110	8	0.10487196436017143
1110	9	0.08121259328856922
1110	10	-0.029618301943877157
1110	11	0.0010179180121596687
1110	12	0.011849341982205885
1110	13	0.01655940949649448
1110	14	-0.0794629493600496
1110	15	-0.0019582257344197274
1110	16	0.09273538684779936
1110	17	0.07464741825303634
1110	18	0.011680721295796559
1110	19	-0.032813436710128485
1110	20	0.024811919397244253
1110	21	0.041112746139880585
1110	22	-0.06876250115976745
1110	23	0.016143349176942934
1110	24	-0.01233153935794462
1110	25	0.018569071130507098
1111	1	-0.10231673647440331
1111	2	-0.1228056565342257
1111	3	-0.19384274457530992
1111	4	-0.06290123519969626
1111	5	-0.17057058827345628
1111	6	-0.130726388519804
1111	7	-0.1465315370159459
1111	8	-0.1473538068604654
1111	9	-0.12468508491463126
1111	10	-0.10433402770382827
1111	11	-0.23333335178232772
1111	12	-0.29364538470914425
1111	13	-0.26587124873096
1111	14	-0.2627711882041339
1111	15	-0.295522332389929
1111	16	-0.3438458065887886
1111	17	-0.3507111330091064
1111	18	-0.29670583701599895
1111	19	-0.2836792932346981
1111	20	-0.24693623137551413
1111	21	-0.24544969808069794
1111	22	-0.2352576454927373
1111	23	-0.21757381986606816
1111	24	-0.3145742601897865
1111	25	-0.2960046351502612
1112	1	-0.06584405140991478
1112	2	-0.047076343897558104
1112	3	-0.08547176223836317
1112	4	-0.15821036983727055
1112	5	-0.03978957801675165
1112	6	-0.12062500473240334
1112	7	-0.13830298656207418
1112	8	-0.1041848381322481
1112	9	-0.10705547801427429
1112	10	-0.13609915103414494
1112	11	-0.13462615053202143
1112	12	-0.14124793691389925
1112	13	-0.15965234983169552
1112	14	-0.16152817619444448
1112	15	-0.2010802390225095
1112	16	-0.13920894807291972
1112	17	-0.0488331461498106
1112	18	-0.16470666210599205
1112	19	-0.2745702655327875
1112	20	-0.24577906763945379
1112	21	-0.3023000813676049
1112	22	-0.20716563191950854
1112	23	-0.17163313510710765
1112	24	-0.17356112172282678
1112	25	-0.15433937847247137
1113	1	-0.2928917474937833
1113	2	-0.23354973472207163
1113	3	-0.132225747342968
1113	4	-0.09729318964358669
1113	5	-0.06171011206731691
1113	6	-0.11003795092501178
1113	7	-0.12791599660261
1113	8	-0.14202606821987018
1113	9	-0.07211207073339665
1113	10	-0.18427325286369722
1113	11	-0.16820190262535561
1113	12	-0.37706478277375055
1113	13	-0.3145867847433621
1113	14	-0.2778042169708641
1113	15	-0.24895403470809185
1113	16	-0.13890054260387785
1113	17	-0.14977924198586123
1113	18	-0.15807176337648343
1113	19	-0.22131328499273195
1113	20	-0.12753991350351157
1113	21	-0.24126801172182866
1113	22	-0.21230517960901807
1113	23	-0.21650258082401308
1113	24	-0.22866225021125786
1113	25	-0.18332113027233277
1114	1	-0.48505053709120105
1114	2	-0.5173810809916652
1114	3	-0.57047534041509
1114	4	-0.5164116023775662
1114	5	-0.604339886151827
1114	6	-0.5160947069285626
1114	7	-0.6471832146043397
1114	8	-0.5592446546069638
1114	9	-0.6879330837764215
1114	10	-0.7114462856611112
1114	11	-0.774186369444358
1114	12	-0.8279738451443919
1114	13	-0.7455569077595499
1114	14	-0.9726323654928706
1114	15	-0.9566885376466701
1114	16	-0.9114031684165269
1114	17	-0.8667118451007241
1114	18	-0.8563357879496962
1114	19	-0.7401724031716057
1114	20	-0.8881376438511879
1114	21	-0.8320288092356805
1114	22	-0.9630771745856208
1114	23	-0.8496600409367048
1114	24	-0.8074324686789751
1114	25	-0.8462765535834649
1115	1	-0.5562597339392639
1115	2	-0.6007350311872166
1115	3	-0.6723862171903612
1115	4	-0.4858614633424949
1115	5	-0.5587310579632501
1115	6	-0.5706681281379744
1115	7	-0.6994367994719397
1115	8	-0.728414636493568
1115	9	-0.6090984501999799
1115	10	-0.6175668454719201
1115	11	-0.6941817348741566
1115	12	-0.7267538249355923
1115	13	-0.730742689741696
1115	14	-0.7591462465883794
1115	15	-0.727726086309687
1115	16	-0.8114096292939976
1115	17	-0.8680378380847387
1115	18	-0.7975071983673914
1115	19	-0.8621289767113893
1115	20	-0.8737687120600484
1115	21	-0.9204448386116297
1115	22	-1.1331164505054048
1115	23	-1.071928189987311
1115	24	-0.8878358565020292
1115	25	-1.0235374105004595
1116	1	-0.30967595291036787
1116	2	-0.495214301325273
1116	3	-0.4450010389318173
1116	4	-0.46278827606211337
1116	5	-0.43211442592575366
1116	6	-0.4850812381541587
1116	7	-0.2871740160301215
1116	8	-0.39662119744971847
1116	9	-0.4064358968748749
1116	10	-0.39818527215268773
1116	11	-0.424896285269476
1116	12	-0.5914208125885736
1116	13	-0.4876905408395231
1116	14	-0.5116053764003112
1116	15	-0.7174750553915885
1116	16	-0.5708953436810873
1116	17	-0.782324838879795
1116	18	-0.6449717821292182
1116	19	-0.7056172850769755
1116	20	-0.6469278330959938
1116	21	-0.7827024462012631
1116	22	-0.940672500758339
1116	23	-0.6777339308630772
1116	24	-0.8353364625254471
1116	25	-0.7205177703327976
1201	1	0.5328532580838043
1201	2	0.6007018638344981
1201	3	0.6663025937844935
1201	4	0.731268748361984
1201	5	0.6414098127820225
1201	6	0.7604147070080884
1201	7	0.729834435906028
1201	8	0.7524119162663752
1201	9	0.7999686551915204
1201	10	0.8177003747674405
1201	11	0.7150360231834156
1201	12	0.7446102000275303
1201	13	0.7786761724126166
1201	14	0.9362997033178928
1201	15	1.0477317746094599
1201	16	0.8905718135591556
1201	17	0.8815134239573581
1201	18	0.8847121972210559
1201	19	0.928945809719707
1201	20	0.8891497649949542
1201	21	0.9108102887463971
1201	22	0.9202532191773898
1201	23	0.9143389326224707
1201	24	0.9117162931479967
1201	25	0.8680564289199353
1202	1	0.4634769155854528
1202	2	0.44290405208543504
1202	3	0.549763707191623
1202	4	0.6490777751780001
1202	5	0.6152353694562791
1202	6	0.5333009606066028
1202	7	0.5182316290494384
1202	8	0.49634621744423413
1202	9	0.5155120015535317
1202	10	0.6580581314951388
1202	11	0.5864069573399888
1202	12	0.5746373296689669
1202	13	0.45489869775796166
1202	14	0.6095144614080539
1202	15	0.5699787919854202
1202	16	0.5965893143395533
1202	17	0.5766903650566988
1202	18	0.6519218383682244
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2210	14	-0.01215963428859368
2210	15	0.06241000207967318
2210	16	0.036221591536723
2210	17	0.02567890895926439
2210	18	0.11152755144165383
2210	19	0.09247910300911855
2210	20	-0.012828436181358427
2210	21	0.004932984074415003
2210	22	-0.009232222045802985
2210	23	-0.03696034521043856
2210	24	0.09142165669278768
2210	25	-0.006463373880045253
2211	1	-0.10501498487287364
2211	2	-0.15447230918583799
2211	3	-0.13101470794639525
2211	4	-0.16259981809080415
2211	5	-0.21327692543476218
2211	6	-0.17597579627993554
2211	7	-0.17283840452768828
2211	8	-0.1666744161852094
2211	9	-0.10407628443616801
2211	10	-0.15321576130173042
2211	11	-0.04636951197294792
2211	12	-0.12772803034450675
2211	13	0.011208250576544287
2211	14	-0.06587351658226481
2211	15	-0.01307992050907103
2211	16	-0.10574133079283854
2211	17	-0.23567167128686606
2211	18	-0.09660367505366452
2211	19	-0.17429336181648836
2211	20	-0.10343520427963426
2211	21	-0.06795855585478705
2211	22	-0.11675184549213924
2211	23	-0.0974717570472663
2211	24	-0.18908854770748462
2211	25	-0.11690957373370026
2212	1	-0.39778830648199204
2212	2	-0.30737159408332815
2212	3	-0.37384523334263875
2212	4	-0.3190933986316722
2212	5	-0.31944048787713797
2212	6	-0.37595945857245994
2212	7	-0.38621113499252147
2212	8	-0.42542256956495805
2212	9	-0.38266719552855477
2212	10	-0.40480596140746883
2212	11	-0.28900881921925503
2212	12	-0.2566456801903101
2212	13	-0.2566551221564879
2212	14	-0.3188290712437123
2212	15	-0.32217664415080804
2212	16	-0.3282457286563272
2212	17	-0.32011306230807435
2212	18	-0.43055312582238514
2212	19	-0.36530483346852805
2212	20	-0.28371476254621086
2212	21	-0.3213523957564348
2212	22	-0.37927363209576015
2212	23	-0.33083159542636054
2212	24	-0.4840939152615107
2212	25	-0.4689058532422834
2213	1	-0.5266070964988003
2213	2	-0.5502215959040306
2213	3	-0.6449033506008206
2213	4	-0.6050185510382207
2213	5	-0.6114558330789066
2213	6	-0.5460429897592931
2213	7	-0.5726832920990717
2213	8	-0.5450947911938471
2213	9	-0.5415368911673966
2213	10	-0.486011165731135
2213	11	-0.34876258491181034
2213	12	-0.5107727904717265
2213	13	-0.41889777424756147
2213	14	-0.5682008143522737
2213	15	-0.5665576138063528
2213	16	-0.4356684008995586
2213	17	-0.542849810068148
2213	18	-0.5273420045080428
2213	19	-0.5313413920053094
2213	20	-0.5796881827826539
2213	21	-0.5002136980964522
2213	22	-0.527621524560665
2213	23	-0.48824360937098277
2213	24	-0.5326351431814871
2213	25	-0.4904461004257499
2214	1	-0.48257888204512156
2214	2	-0.5922395967302947
2214	3	-0.6070681106374458
2214	4	-0.6834125947337952
2214	5	-0.5985709978663252
2214	6	-0.6344615474846123
2214	7	-0.6890636257510536
2214	8	-0.5984402626879728
2214	9	-0.5802413456076181
2214	10	-0.6328066341109846
2214	11	-0.5349983498792241
2214	12	-0.5501602797410072
2214	13	-0.6807844608756923
2214	14	-0.7814010341270077
2214	15	-0.6005804211517116
2214	16	-0.6840346086691227
2214	17	-0.721205323979639
2214	18	-0.7900883521523099
2214	19	-0.8650019501741468
2214	20	-0.84603904363221
2214	21	-0.8774048632966966
2214	22	-0.8013258420930285
2214	23	-0.7536947147590496
2214	24	-0.8415073293111845
2214	25	-0.7800515139130724
2215	1	-0.746069740540122
2215	2	-0.6670895788027593
2215	3	-0.8919039867816281
2215	4	-0.8225949026025532
2215	5	-0.8474449639603705
2215	6	-0.8172834019990916
2215	7	-0.788099541401877
2215	8	-0.8814940253622723
2215	9	-1.0791954491278162
2215	10	-1.0079936491796886
2215	11	-0.9139764964197568
2215	12	-0.8892340083752757
2215	13	-0.8943208308733261
2215	14	-1.0452075641736371
2215	15	-0.9896933977914273
2215	16	-1.0729126145396037
2215	17	-1.0900953349717426
2215	18	-1.1398075677166375
2215	19	-1.1583952717292405
2215	20	-1.1243179652024224
2215	21	-0.9450606580584378
2215	22	-1.0815311859572319
2215	23	-0.9539354420048483
2215	24	-1.0906934587838677
2215	25	-1.0627844708117635
2216	1	-0.3748345924082983
2216	2	-0.4802803008513763
2216	3	-0.4574458933474759
2216	4	-0.3813435608645648
2216	5	-0.33107259503242403
2216	6	-0.2689205048527228
2216	7	-0.3816848579862153
2216	8	-0.3313156874550529
2216	9	-0.29047223556764834
2216	10	-0.3344568451974794
2216	11	-0.11756032062165644
2216	12	-0.30153030624954624
2216	13	-0.16835927641638904
2216	14	-0.2644369897907026
2216	15	-0.24501760680778517
2216	16	-0.4412193899678769
2216	17	-0.6185362070065921
2216	18	-0.4705004652088718
2216	19	-0.41715648419483387
2216	20	-0.3494733578791198
2216	21	-0.42312580755339013
2216	22	-0.3404441738373336
2216	23	-0.5007259401123179
2216	24	-0.6684226606632393
2216	25	-0.5622378423574261
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	196715.0
3	4.0
4	1698.0
5	9183.0
6	11038.0
7	11011.0
8	9480.0
9	9013.0
10	9051.0
11	10160.0
12	15885.0
13	12387.0
14	6971.0
15	7138.0
16	7447.0
17	8067.0
18	8829.0
19	10467.0
20	13472.0
21	16506.0
22	20298.0
23	24020.0
24	28269.0
25	32718.0
26	36777.0
27	41883.0
28	47723.0
29	57743.0
30	73248.0
31	92903.0
32	120584.0
33	159496.0
34	219235.0
35	309474.0
36	462040.0
37	857377.0
38	2352826.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.40704682516712	17.38514781759406	12.420524297820469	25.787281059418344
2	17.12386517567583	20.06554028302223	37.37082271215776	25.43977182914417
3	18.873460719465708	23.87975081054472	27.947770554074978	29.29901791591459
4	13.013497450540253	16.06264338294038	36.66485126092097	34.2590079055984
5	15.93708885030402	34.95248936846463	32.6703002671913	16.44012151404004
6	33.296122267836395	36.24568799115074	15.995105849937477	14.463083891075392
7	29.843649978203608	30.23896154980824	20.878136504567067	19.03925196742108
8	27.24127725208172	32.09639281467991	19.442014668832208	21.22031526440616
9	27.351335443071367	14.933078999112945	18.46126917398405	39.254316383831636
10	16.683353461740637	25.647308853924283	30.121335672122374	27.548002012212695
11	36.964775699733345	21.870323404749023	21.75707088879528	19.407830006722353
12	25.48519882084786	23.514443400924616	27.624970343065357	23.37538743516217
13	29.651648205466767	19.876339585014307	24.427603002656337	26.044409206862586
14	24.15868744079417	20.009925829156355	24.445130421946278	31.386256308103196
15	25.3039408993016	26.38553977140832	22.3580587425468	25.952460586743275
16	25.77120716660713	26.122411850599665	22.918777757668387	25.187603225124818
17	25.38831973794991	25.14726200214926	23.89761233269644	25.56680592720439
18	26.889041259964763	23.837620949279383	24.660292097626648	24.6130456931292
19	27.40112592855374	24.417985672997858	24.002039838245604	24.178848560202802
20	26.42424878746208	24.301700964420597	23.983916721121584	25.29013352699574
21	26.862509344452878	24.356367350457962	23.842249528395293	24.938873776693868
22	25.92468153343013	24.145907736318662	24.552255578257824	25.377155151993385
23	24.863834845774228	23.592486389168783	24.77364544424154	26.77003332081545
24	25.18681764305158	25.170975923808154	24.277055752050565	25.365150681089705
25	25.203420561530375	24.368800819612957	24.472578796512742	25.95519982234393
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2318.0
1	2318.0
2	2466.0
3	2614.0
4	2614.0
5	2614.0
6	4595.5
7	6577.0
8	6577.0
9	6577.0
10	9520.5
11	12464.0
12	12464.0
13	12464.0
14	17724.0
15	22984.0
16	22984.0
17	22984.0
18	37249.5
19	51515.0
20	51515.0
21	51515.0
22	72915.0
23	94315.0
24	94315.0
25	94315.0
26	128615.5
27	162916.0
28	162916.0
29	162916.0
30	195985.5
31	229055.0
32	229055.0
33	229055.0
34	284785.0
35	340515.0
36	340515.0
37	340515.0
38	383000.0
39	425485.0
40	425485.0
41	425485.0
42	483043.0
43	540601.0
44	540601.0
45	540601.0
46	578320.0
47	616039.0
48	616039.0
49	616039.0
50	630923.0
51	645807.0
52	645807.0
53	645807.0
54	604659.0
55	563511.0
56	563511.0
57	563511.0
58	531393.0
59	499275.0
60	499275.0
61	499275.0
62	445593.5
63	391912.0
64	391912.0
65	391912.0
66	334570.0
67	277228.0
68	277228.0
69	277228.0
70	221360.0
71	165492.0
72	165492.0
73	165492.0
74	138060.0
75	110628.0
76	110628.0
77	110628.0
78	94334.5
79	78041.0
80	78041.0
81	78041.0
82	59951.5
83	41862.0
84	41862.0
85	41862.0
86	31219.0
87	20576.0
88	20576.0
89	20576.0
90	14558.0
91	8540.0
92	8540.0
93	8540.0
94	4640.5
95	741.0
96	741.0
97	741.0
98	433.0
99	125.0
100	125.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14066670482548366
2	0.15896787429280668
3	0.17220421393841168
4	0.19221876449784
5	0.1824656721273942
6	0.0673114000469956
7	0.05494116512926801
8	0.05925286040500563
9	0.0693636916847921
10	0.06676537750115982
11	0.6815491073849361
12	0.6424990811758539
13	0.6390723189916433
14	0.6400702222650673
15	0.6409363269929447
16	0.6484676724527483
17	0.639731311719376
18	0.6504069939086479
19	0.6448149699047435
20	0.6514613822730203
21	0.6390534906279938
22	0.642612051357751
23	0.6280012411657317
24	0.6388652069914986
25	0.6311832346224988
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	5311136.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.188970481572035
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.9873757830205	40.472651724326724
2	10.292503806817589	9.919703242564012
3	2.3306565577950926	3.3693582019881
4	0.938991445048306	1.8099612411152595
5	0.524698935416071	1.2642350755238656
6	0.3190646742168141	0.9225238900527871
7	0.22548728724427433	0.7606200160288863
8	0.16611534704884023	0.6403942044378125
9	0.1345734947998009	0.5836462351658634
>10	0.8392953926031084	7.994224032288868
>50	0.1115770687560938	3.743660836653743
>100	0.10134591941604833	9.9266744595256
>500	0.015080009884359305	5.025667831357711
>1k	0.01256667340841994	11.550795351320163
>5k	6.283336704209799E-4	1.7997690602796281
>10k+	3.9270854401311245E-5	0.2161145973712289
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAAGGGCAGGGACTTAATCAA	11420	0.21501991287739575	No Hit
GTATCTGATCGTCTTCGAACCTCCG	7045	0.13264582191079272	No Hit
GAATAGGACCGCGGTTCTATTTTGT	6946	0.13078181390949128	No Hit
GTCCTATTCCATTATTCCTAGCTGC	6514	0.1226479608129033	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	6147	0.1157379513535334	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	6121	0.11524841389864618	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	6081	0.11449527935266579	No Hit
GTATCAACGCAGAGTACGGGCGGTG	5993	0.11283838335150898	No Hit
CTATTGGAGCTGGAATTACCGCGGC	5940	0.111840480078085	No Hit
GACTTAATCAACGCAAGCTTATGAC	5793	0.10907271062160712	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	5639	0.10617314261958272	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	5638	0.1061543142559332	No Hit
CTTATGACCCGCACTTACTGGGAAT	5596	0.1053635229826538	No Hit
GAACTACGACGGTATCTGATCGTCT	5589	0.10523172443710722	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	5578	0.10502461243696265	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	5474	0.10306646261741367	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8828363649509258E-5
2	0.0	0.0	0.0	0.0	1.8828363649509258E-5
3	0.0	0.0	0.0	0.0	1.8828363649509258E-5
4	0.0	0.0	0.0	3.7656727299018516E-5	1.8828363649509258E-5
5	0.0	0.0	0.0	3.7656727299018516E-5	1.8828363649509258E-5
6	0.0	0.0	0.0	3.7656727299018516E-5	3.7656727299018516E-5
7	0.0	0.0	0.0	3.7656727299018516E-5	3.7656727299018516E-5
8	0.0	0.0	0.0	3.7656727299018516E-5	3.7656727299018516E-5
9	0.0	0.0	0.0	3.7656727299018516E-5	3.7656727299018516E-5
10	0.0	0.0	0.0	3.7656727299018516E-5	3.7656727299018516E-5
11	0.0	0.0	0.0	3.7656727299018516E-5	3.7656727299018516E-5
12	0.0	0.0	0.0	3.7656727299018516E-5	7.531345459803703E-5
13	0.0	0.0	0.0	3.7656727299018516E-5	9.414181824754629E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2925	0.0	15.853378	1
GTATCAA	10025	0.0	13.414093	1
CGTCGTA	990	0.0	12.009168	10
ACGCTTA	210	0.0	11.772386	17
CGAACGA	535	0.0	11.552999	16
ACCGTCC	315	0.0	11.473903	8
ACGGTAT	1080	0.0	11.360674	9
ATACCGT	1135	0.0	11.228729	6
TACCGTC	1110	0.0	11.22447	7
GCGTTAT	405	0.0	11.215995	1
CGGTTCT	1335	0.0	11.040629	12
GTCGTAG	1130	0.0	10.94216	11
GTCCTAT	1425	0.0	10.891321	1
GGTTCTA	1385	0.0	10.779674	13
GCTCGTA	540	0.0	10.744203	9
GCGCGCT	515	0.0	10.709173	15
CCGTCGT	1200	0.0	10.70017	9
AACCGCG	555	0.0	10.624689	7
CTCGACG	235	0.0	10.521102	13
ACTCTAA	1155	0.0	10.45827	10
>>END_MODULE