FastQCFastQC Report
Thu 2 Feb 2017
SRR4050279_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050279_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5311136
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA109200.2056057310526411No Hit
GTATCAACGCAGAGTACTTTTTTTT72840.13714580082302544No Hit
GAATAGGACCGCGGTTCTATTTTGT69620.13108306772788347No Hit
GATTAAGAGGGACGGCCGGGGGCAT65320.12298687135859447No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG61810.11637811571761673No Hit
ATACAGGACTCTTTCGAGGCCCTGT60250.11344089098829328No Hit
CTATTGGAGCTGGAATTACCGCGGC59950.112876040078808No Hit
GTATCTGATCGTCTTCGAACCTCCG59640.11229236080567322No Hit
CTTATGACCCGCACTTACTGGGAAT58820.11074843498641346No Hit
GTCCTATTCCATTATTCCTAGCTGC58590.11031538262247474No Hit
GACTTAATCAACGCAAGCTTATGAC57560.10837606116657528No Hit
ATCAGATACCGTCGTAGTTCCGACC56330.10606017243768565No Hit
GGGTAGGCACACGCTGAGCCAGTCA56170.1057589186192935No Hit
GAACTACGACGGTATCTGATCGTCT55970.1053823513463033No Hit
TCGTAGTTCCGACCATAAACGATGC53990.10165433534370048No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC53360.10046814843378139No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28700.015.6859071
GTATCAA93800.013.3125551
CTCGACG2100.012.65712613
CGCTTAC2000.012.34058318
CGACCAT10200.011.822638510
ACGCTTA2100.011.75293517
TAGATCG1406.8030204E-1011.5365835
CGCCAGT8900.011.41263918
AACCGCG7250.011.3958137
GCATCGC9200.011.24685214
TCGACGC2450.011.23632314
CGAACGA4650.011.2279916
CGTCGTA8350.011.14424110
ATCGCCA9200.011.1436716
AGAACCG8450.011.1310165
AACCGTC600.005894925411.0792627
CCGACCA11050.010.9997579
ATACCGT9100.010.8558146
CGGTTCT12950.010.70477712
TCGCGTA5950.010.6928459
Produced by FastQC (version 0.11.5)