FastQCFastQC Report
Thu 2 Feb 2017
SRR4050278_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050278_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745250
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA172542.3151962428715196No Hit
CTTCCGTACTCTGCGTTGATACCAC106151.4243542435424354No Hit
GAGTACGGAAGCAGTGGTATCAACG87481.1738342837973834No Hit
TCCGTACTCTGCGTTGATACCACTG83871.1253941630325395No Hit
GTACATGGGAAGCAGTGGTATCAAC64780.8692385105669239No Hit
GCTTCCGTACTCTGCGTTGATACCA56950.7641730962764173No Hit
CCCATGTACTCTGCGTTGATACCAC55720.7476685675947669No Hit
GCGTTGATACCACTGCTTCCGTACT42670.5725595437772559No Hit
GTATCAACGCAGAGTACGGAAGCAG36780.4935256625293526No Hit
ACTCTGCGTTGATACCACTGCTTCC27520.3692720563569272No Hit
TATCAACGCAGAGTACGGAAGCAGT26630.357329755115733No Hit
GAGTACATGGGAAGCAGTGGTATCA21020.2820530023482053No Hit
GTACATGGAAGCAGTGGTATCAACG20460.2745387453874539No Hit
CAGTGGTATCAACGCAGAGTACGGA20030.2687688695068769No Hit
CATGTACTCTGCGTTGATACCACTG18860.25306943978530694No Hit
GGTATCAACGCAGAGTACGGAAGCA18800.2522643408252264No Hit
ATACCACTGCTTCCGTACTCTGCGT18550.248909761824891No Hit
TCCATGTACTCTGCGTTGATACCAC18400.2468970144246897No Hit
ACGCAGAGTACGGAAGCAGTGGTAT18060.24233478698423347No Hit
GTATCAACGCAGAGTACATGGGAAG17280.23186850050318686No Hit
GCGTTGATACCACTGCTTCCCATGT15760.21147266018114724No Hit
GTATCAACGCAGAGTACTTTTTTTT14020.1881247903388125No Hit
GTACTCTGCGTTGATACCACTGCTT13310.17859778597785977No Hit
AAGCAGTGGTATCAACGCAGAGTAC13310.17859778597785977No Hit
GTATCTGATCGTCTTCGAACCTCCG13160.1765850385776585No Hit
GTGGTATCAACGCAGAGTACGGAAG13130.17618248909761824No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG12680.17014424689701443No Hit
TATCAACGCAGAGTACATGGGAAGC12670.170010063737001No Hit
GAAGCAGTGGTATCAACGCAGAGTA12300.16504528681650452No Hit
GATTAAGAGGGACGGCCGGGGGCAT11940.16021469305602146No Hit
GAATAGGACCGCGGTTCTATTTTGT11930.16008050989600806No Hit
CTATTGGAGCTGGAATTACCGCGGC11710.15712848037571284No Hit
GTCCTATTCCATTATTCCTAGCTGC11530.1547131834954713No Hit
ACGCAGAGTACATGGGAAGCAGTGG10880.14599127809459914No Hit
GTTGATACCACTGCTTCCGTACTCT10600.14223414961422343No Hit
CTCTTAATCATGGCCTCAGTTCCGA10070.13512244213351224No Hit
GTCTTGCGCCGGTCCAAGAATTTCA10070.13512244213351224No Hit
CTTTAATATACGCTATTGGAGCTGG10050.1348540758134854No Hit
ATACAGGACTCTTTCGAGGCCCTGT9800.13149949681314996No Hit
GATACCACTGCTTCCGTACTCTGCG9770.1310969473331097No Hit
GAATAACGCCGCCGCATCGCCAGTC9770.1310969473331097No Hit
ATCAACGCAGAGTACGGAAGCAGTG9670.12975511573297552No Hit
CCACTGCTTCCGTACTCTGCGTTGA9560.1282791009728279No Hit
GAACTACGACGGTATCTGATCGTCT9400.1261321704126132No Hit
GTCCTGTATTGTTATTTTTCGTCAC9330.1251928882925193No Hit
GCTTCCCATGTACTCTGCGTTGATA9240.12398523985239851No Hit
GCGCAAGACGGACCAGAGCGAAAGC8980.12049647769204964No Hit
GGTATCAACGCAGAGTACATGGGAA8940.11995974505199598No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC8720.11700771553170078No Hit
ATCAGATACCGTCGTAGTTCCGACC8540.11459241865145924No Hit
CTGCTTCCGTACTCTGCGTTGATAC8520.11432405233143239No Hit
GTACAAAGGGCAGGGACTTAATCAA8320.11164038913116403No Hit
CGGAAGCAGTGGTATCAACGCAGAG8290.1112378396511238No Hit
ATATTAAAGTTGCTGCAGTTAAAAA8210.11016437437101645No Hit
TCGTAGTTCCGACCATAAACGATGC8160.10949345857094933No Hit
CAGTGGTATCAACGCAGAGTACATG8060.10815162697081517No Hit
GGGTAGGCACACGCTGAGCCAGTCA7870.1056021469305602No Hit
GAATAATGGAATAGGACCGCGGTTC7800.10466286481046627No Hit
GAATAATTGCAATCCCCGATCCCCA7770.10426031533042604No Hit
CTCTAGATAACCTCGGGCCGATCGC7750.1039919490103992No Hit
TATCAACGCAGAGTACTTTTTTTTT7740.10385776585038577No Hit
GCTTTGAACACTCTAATTTTTTCAA7620.10224756793022476No Hit
GAGTACATGGAAGCAGTGGTATCAA7470.10023482053002349No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTTA350.002161320316.29409617
GAGGTTA704.3710133E-714.9453245
TTCGGAA1058.731149E-1114.4836418
CGCTTAC400.005257684314.25733418
AGAATTT1900.014.00720517
CGCCAGT1301.8189894E-1213.89176118
TGTACCG551.939437E-413.8336885
TAGAGTG707.1667837E-613.58665855
AAATCGC707.2118764E-613.57841314
GCTCGTA852.65607E-713.4268159
GGCGGGA500.001491702313.30774312
TCGCAGT500.001492460613.30684516
GCGCGCT1009.966243E-913.30684515
CGCGCTA1009.966243E-913.30684516
GGTTTTC1153.7289283E-1013.22419314
GTTTTCG1153.7289283E-1013.22419315
TTTTCGG1153.7289283E-1013.22419316
AAGCTCG905.3193435E-712.68088157
AAGAATT2250.012.67318516
CGCAGTT604.0698433E-412.67318517
Produced by FastQC (version 0.11.5)