##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050278_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	745250
Sequences flagged as poor quality	0
Sequence length	25
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.302228782287823	31.0	31.0	34.0	27.0	34.0
2	30.415255283461924	31.0	31.0	34.0	27.0	34.0
3	30.54250117410265	31.0	31.0	34.0	27.0	34.0
4	34.12405501509561	37.0	35.0	37.0	30.0	37.0
5	34.10916470982892	37.0	35.0	37.0	30.0	37.0
6	34.10016236162362	37.0	35.0	37.0	30.0	37.0
7	33.97369339147937	37.0	35.0	37.0	30.0	37.0
8	33.909706809795374	37.0	35.0	37.0	30.0	37.0
9	35.41466487755787	39.0	37.0	39.0	30.0	39.0
10	35.31647500838645	39.0	35.0	39.0	30.0	39.0
11	35.166248909761826	39.0	35.0	39.0	29.0	39.0
12	35.08536464273734	39.0	35.0	39.0	28.0	39.0
13	35.087867158671585	39.0	35.0	39.0	28.0	39.0
14	36.22649043944985	40.0	37.0	41.0	28.0	41.0
15	36.17929419657833	40.0	37.0	41.0	28.0	41.0
16	36.126749412948676	40.0	37.0	41.0	28.0	41.0
17	36.1250063737001	40.0	37.0	41.0	28.0	41.0
18	36.07885541764509	40.0	37.0	41.0	27.0	41.0
19	36.08755048641395	40.0	37.0	41.0	27.0	41.0
20	36.03765984568937	40.0	37.0	41.0	27.0	41.0
21	35.94987856424019	40.0	37.0	41.0	27.0	41.0
22	35.889913451861794	40.0	36.0	41.0	27.0	41.0
23	35.79966454209997	40.0	36.0	41.0	27.0	41.0
24	35.71464072458907	40.0	36.0	41.0	27.0	41.0
25	35.5080214693056	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.6901333200697763
1101	2	0.6388611598955265
1101	3	0.6533745422381081
1101	4	0.6421323953708296
1101	5	0.8444896772568597
1101	6	0.751331946966225
1101	7	0.8602711641285339
1101	8	0.9015833556329085
1101	9	1.0441733200780305
1101	10	1.0177667305715232
1101	11	0.7296438874084004
1101	12	0.8035823359498266
1101	13	0.9568343729520876
1101	14	1.054166959389235
1101	15	1.1311383127593686
1101	16	1.1205652731147495
1101	17	1.015172690462407
1101	18	1.1058878191741854
1101	19	1.2065867498863128
1101	20	1.2198634224473324
1101	21	1.265004996338206
1101	22	1.2412223735131107
1101	23	1.3381577186895228
1101	24	1.2391788227889293
1101	25	1.345632383154701
1102	1	0.5141388369106572
1102	2	0.7028605469132074
1102	3	0.6105561140538285
1102	4	0.5321391704385761
1102	5	0.6410174550346355
1102	6	0.7063777593291078
1102	7	0.6732825204325792
1102	8	0.6205946796747241
1102	9	0.7524259978429058
1102	10	0.48232990001274345
1102	11	0.6439118574690568
1102	12	0.8664928727932875
1102	13	0.8782524591567622
1102	14	0.7739566096666906
1102	15	0.9354735495641151
1102	16	0.9176648988729141
1102	17	1.0080370923207127
1102	18	1.068416048623142
1102	19	0.9356404987571381
1102	20	1.0683172551999434
1102	21	1.116589878258452
1102	22	1.056790511337347
1102	23	1.0039178167666876
1102	24	1.1286879529992788
1102	25	1.1870248208886025
1103	1	0.834825377109393
1103	2	0.7724179091534964
1103	3	0.8083880923736118
1103	4	0.936836550745717
1103	5	0.9942243204366221
1103	6	0.9231799273507946
1103	7	0.941575203359406
1103	8	1.0785892596205215
1103	9	1.146283269748956
1103	10	1.0424021674020807
1103	11	1.1190699423832413
1103	12	1.0453546614111744
1103	13	1.2350726940257744
1103	14	1.3611291480587369
1103	15	1.347397668805307
1103	16	1.389209614320066
1103	17	1.3946676252927048
1103	18	1.440593103727032
1103	19	1.3430479061645428
1103	20	1.5199884385784443
1103	21	1.5438708177345148
1103	22	1.50123495578179
1103	23	1.4013884581397704
1103	24	1.5342886756731673
1103	25	1.4173771244116367
1104	1	0.528624774933931
1104	2	0.5009393807544207
1104	3	0.4787908437280315
1104	4	0.538243263030779
1104	5	0.6406279331727234
1104	6	0.49528462006000495
1104	7	0.5589784384493086
1104	8	0.6485761251333457
1104	9	0.6508779651262344
1104	10	0.8092654159352364
1104	11	0.8010395929298753
1104	12	0.6644006970365908
1104	13	0.7073766604192215
1104	14	0.6613974589510221
1104	15	0.8321845441353517
1104	16	0.7888606280665442
1104	17	0.7580353109707332
1104	18	0.8797101241698044
1104	19	0.80644433095555
1104	20	0.8129999255148164
1104	21	0.9027186247343479
1104	22	0.9581702262738432
1104	23	1.0031664829314124
1104	24	0.9337504704663999
1104	25	1.030601708308076
1105	1	0.6553180121985562
1105	2	0.5282669929228234
1105	3	0.707873256891066
1105	4	0.7132442205636309
1105	5	0.61988063698265
1105	6	0.5699939619007353
1105	7	0.6308091736768304
1105	8	0.5720440913410769
1105	9	0.6904274515506685
1105	10	0.8237282925742164
1105	11	0.8635540967363511
1105	12	0.7426653020411464
1105	13	0.8380246961250606
1105	14	0.9506842809708189
1105	15	0.924707752103231
1105	16	1.0098081424990042
1105	17	0.8287155047603605
1105	18	1.0329604412821567
1105	19	1.0920301946163917
1105	20	1.1524186617667098
1105	21	1.0098398904500954
1105	22	0.9184885627651624
1105	23	0.9326582695525403
1105	24	0.9967906785852279
1105	25	1.0951672099388858
1106	1	0.5428348835277781
1106	2	0.5341976099261814
1106	3	0.5563788340836027
1106	4	0.5542032108214698
1106	5	0.525900621462867
1106	6	0.6063595374015378
1106	7	0.6061172708115521
1106	8	0.6398765806482416
1106	9	0.7584283783245027
1106	10	0.831684572386358
1106	11	1.0181708647229328
1106	12	0.8153427590031654
1106	13	0.8654218959441167
1106	14	0.9947862113818786
1106	15	0.9000415929555672
1106	16	0.883026953065702
1106	17	0.7042093244293
1106	18	0.8426099221784753
1106	19	1.0073914709163532
1106	20	0.9767648632811756
1106	21	0.9316473936404748
1106	22	0.9868462239116411
1106	23	0.8644146162983475
1106	24	1.0684361802383435
1106	25	1.1151296241111694
1107	1	0.20894385275742877
1107	2	0.3457164867720408
1107	3	0.29066267783132815
1107	4	0.2003951072352379
1107	5	0.24544306708736485
1107	6	0.40060555471441006
1107	7	0.1646900503270814
1107	8	0.14521229025761073
1107	9	0.31463336850418244
1107	10	0.2934325901356871
1107	11	0.39759182929702064
1107	12	0.4809279775963162
1107	13	0.5300032591506323
1107	14	0.3943606639897155
1107	15	0.630033591209731
1107	16	0.6471391229936856
1107	17	0.6213621577747546
1107	18	0.6901039208690989
1107	19	0.5898494617507168
1107	20	0.4088918728105284
1107	21	0.5595116549338499
1107	22	0.6611678940942269
1107	23	0.5328913748638513
1107	24	0.6548113893748848
1107	25	0.6102812935663238
1108	1	0.18057678006247002
1108	2	0.36652146314726153
1108	3	0.3723856249320576
1108	4	0.5008387039812305
1108	5	0.35343575568823127
1108	6	0.20759184222868043
1108	7	0.1928707256922948
1108	8	0.29832324724172565
1108	9	0.2295039740787601
1108	10	0.24929760379037447
1108	11	0.4736260454890271
1108	12	0.2942358799761351
1108	13	0.2892154775678506
1108	14	0.46633965455425397
1108	15	0.35199524248778147
1108	16	0.4293309532998677
1108	17	0.41281533447653374
1108	18	0.4406970689610574
1108	19	0.3024413960926182
1108	20	0.3697332616873794
1108	21	0.4670289580968259
1108	22	0.31754224928950947
1108	23	0.34571160981118254
1108	24	0.42696883009620024
1108	25	0.36305413446392265
1109	1	-0.1821704281621912
1109	2	-0.24809958975086843
1109	3	-0.19514030924704073
1109	4	-0.2815062184905557
1109	5	-0.05507715442631422
1109	6	0.007508278885133279
1109	7	-0.1041442059798996
1109	8	-0.23741063305167387
1109	9	-1.4539750801390028E-4
1109	10	0.04975635810570367
1109	11	0.17222403828063193
1109	12	0.16959777677962506
1109	13	-0.04658677273391021
1109	14	-0.062122526702232506
1109	15	-0.06616543547259823
1109	16	0.11975197749947597
1109	17	0.08128831988248919
1109	18	-0.049908385446279624
1109	19	0.011248466057928397
1109	20	0.05656486044827602
1109	21	0.03304188931321761
1109	22	0.1125271825654437
1109	23	0.1152793594250241
1109	24	0.1279724342929356
1109	25	0.2005593044182561
1110	1	-0.36485823201598677
1110	2	-0.2697105599832881
1110	3	-0.22362382644051948
1110	4	-0.12089778407452201
1110	5	-0.15796750055717013
1110	6	-0.2948461001090479
1110	7	-0.21372673845826995
1110	8	-0.16958158260992207
1110	9	-0.07998188849806098
1110	10	-0.3059107338889788
1110	11	-0.19826627339327274
1110	12	-0.12131716300146422
1110	13	-0.10371532408822759
1110	14	-0.019277459161223476
1110	15	-0.0066020158353623515
1110	16	0.016457838950216797
1110	17	0.11947093096578243
1110	18	0.2082226478854423
1110	19	0.11105249213753865
1110	20	0.02051158138464615
1110	21	-0.02704376851846746
1110	22	0.02347884053711624
1110	23	0.16337911230406377
1110	24	0.0039058677667895836
1110	25	0.007028328155868735
1111	1	-0.14829966548399653
1111	2	-0.20400601506434413
1111	3	-0.25128035342802235
1111	4	-0.0683210556725129
1111	5	-0.18571698405935422
1111	6	-0.1883951798395529
1111	7	-0.33286687760500655
1111	8	-0.261408217400799
1111	9	-0.4120867120247027
1111	10	-0.3750101554637908
1111	11	-0.16861052671679744
1111	12	-0.3832856415782615
1111	13	-0.23882172656637124
1111	14	-0.14799997480017169
1111	15	-0.2136750901298754
1111	16	-0.29424211051286164
1111	17	-0.3342354154765985
1111	18	-0.317396691259205
1111	19	-0.31618612330790796
1111	20	-0.2555679579700012
1111	21	-0.2552297548656455
1111	22	-0.2690304354756563
1111	23	-0.34430616574065454
1111	24	-0.36512494928170014
1111	25	-0.34915092342635745
1112	1	-0.11940720962524765
1112	2	-0.05312319002421617
1112	3	-0.03940234379720309
1112	4	-0.059492805271226246
1112	5	-0.02440077218134462
1112	6	-0.18438233874805832
1112	7	-0.08968870746054591
1112	8	-0.2758544453301681
1112	9	-0.3599197778352945
1112	10	-0.10936007520697189
1112	11	-0.10279993281631761
1112	12	-0.28376718250924426
1112	13	-0.3319196398890014
1112	14	-0.11991008715972384
1112	15	-0.19922886220050628
1112	16	-0.41783761613083925
1112	17	-0.3149737782374302
1112	18	-0.2676374617246893
1112	19	-0.36835305749731617
1112	20	-0.3109451650325994
1112	21	-0.45587180944028205
1112	22	-0.6237655879636179
1112	23	-0.5922997451949144
1112	24	-0.578608095349118
1112	25	-0.4791669747907221
1113	1	-0.4974168404438686
1113	2	-0.40464806963898425
1113	3	-0.4262402250171071
1113	4	-0.23204497220541498
1113	5	-0.440131109259994
1113	6	-0.7180902039166028
1113	7	-0.4981959305745107
1113	8	-0.3657420857796083
1113	9	-0.3438684134693233
1113	10	-0.5226827076307217
1113	11	-0.2793649408420009
1113	12	-0.44829366726044384
1113	13	-0.5181154665342689
1113	14	-0.4465553520072376
1113	15	-0.33406032287466303
1113	16	-0.3889451602720868
1113	17	-0.5083927188473822
1113	18	-0.6512650700714033
1113	19	-0.4895408584603942
1113	20	-0.4570125807629424
1113	21	-0.7159039121690185
1113	22	-0.8436692797774228
1113	23	-0.7672596167839956
1113	24	-0.8129580150922919
1113	25	-0.6653628014359896
1114	1	-0.5742816139332625
1114	2	-0.43122338046190833
1114	3	-0.438694141326895
1114	4	-0.4552613086357127
1114	5	-0.5357791289573726
1114	6	-0.9387918703448364
1114	7	-0.9559039322387122
1114	8	-0.9411985915695169
1114	9	-1.324446000094845
1114	10	-1.0352527764954118
1114	11	-0.7877942039360661
1114	12	-0.9145208354086591
1114	13	-0.9466823015018306
1114	14	-0.955779308570115
1114	15	-1.05925781502944
1114	16	-0.8613264451424669
1114	17	-0.8978653599989883
1114	18	-0.929235852125899
1114	19	-0.9570988592714968
1114	20	-1.0447792886845448
1114	21	-1.1531697204004914
1114	22	-1.1664067001932352
1114	23	-0.9756753615581175
1114	24	-1.1226502567578578
1114	25	-1.2091450961117403
1115	1	-0.4266790533366347
1115	2	-0.5389112397232019
1115	3	-0.5491275428327853
1115	4	-0.6282700233114085
1115	5	-0.42533413425273636
1115	6	-0.5933541009308314
1115	7	-0.8393684164743433
1115	8	-0.8194468183215733
1115	9	-0.9572266560925868
1115	10	-0.857093271811415
1115	11	-0.8987949902277208
1115	12	-0.842578899603609
1115	13	-0.9456982435140162
1115	14	-0.9876476480217065
1115	15	-1.1105072549756514
1115	16	-1.1551615230248942
1115	17	-0.9904711496424454
1115	18	-0.9627579272811957
1115	19	-1.0425270949844148
1115	20	-1.1476757470987167
1115	21	-1.2524552383812022
1115	22	-1.044135960813115
1115	23	-1.0255888558368724
1115	24	-1.0377982712179872
1115	25	-1.0024970103875361
1116	1	-0.43322517281191253
1116	2	-0.22127731989501598
1116	3	-0.3060756295831979
1116	4	-0.1407857031400539
1116	5	-0.05199514519446069
1116	6	-0.4462871006528246
1116	7	-0.568058896418016
1116	8	-0.5635195326014184
1116	9	-0.6274434365902763
1116	10	-0.4894988560755067
1116	11	-0.5225918350592806
1116	12	-0.5363978227803301
1116	13	-0.40606756617408024
1116	14	-0.860683310615677
1116	15	-0.8750666864224357
1116	16	-1.0221650492547454
1116	17	-0.6676257467819084
1116	18	-1.4486869230938595
1116	19	-1.0085478434699269
1116	20	-0.9421875921165679
1116	21	-1.0942334670884009
1116	22	-0.9891961340961188
1116	23	-0.8108318083927912
1116	24	-0.950917928970199
1116	25	-0.9831108667225692
1201	1	0.6515379426748922
1201	2	0.6186870889169995
1201	3	0.5775869634802326
1201	4	0.5693038625175433
1201	5	0.686822035492682
1201	6	0.7205295810153416
1201	7	0.7636175701977166
1201	8	0.776090717083342
1201	9	0.8616157786095968
1201	10	0.6918955720271001
1201	11	0.7722840442812355
1201	12	0.8909449114930936
1201	13	0.7765455404974162
1201	14	0.9989335789083285
1201	15	1.0663728183527823
1201	16	1.0110661089126154
1201	17	1.122692460296534
1201	18	1.0507516805603245
1201	19	0.9930966508764101
1201	20	1.0427634803102634
1201	21	1.1469782829525812
1201	22	1.0560465345006378
1201	23	1.153983229812063
1201	24	1.1642760646559722
1201	25	1.2564398924770614
1202	1	0.6731368593233142
1202	2	0.6484573632417288
1202	3	0.5254466143101801
1202	4	0.7681459088843425
1202	5	0.7028793168964995
1202	6	0.7924516380859146
1202	7	0.6651939440513175
1202	8	0.6217812320807852
1202	9	0.9015092274139391
1202	10	0.9135196263244154
1202	11	0.8749624202269359
1202	12	0.9044430180605119
1202	13	0.8160927384604548
1202	14	0.9189674741897491
1202	15	0.8576103204813776
1202	16	0.7688599964094749
1202	17	0.9193313133710319
1202	18	0.8337594407958022
1202	19	0.690483146282709
1202	20	0.8518373606712686
1202	21	0.7659782910615007
1202	22	0.8325833773741138
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2210	21	-0.3543607672085898
2210	22	-0.41052228986948336
2210	23	-0.26040549970128524
2210	24	-0.47234621826197554
2210	25	-0.23573050682887953
2211	1	0.17026797101753388
2211	2	0.1659155981756939
2211	3	-0.003745198862215915
2211	4	-0.05556177938645135
2211	5	0.0032073147973079585
2211	6	0.07960286698472174
2211	7	0.15461929172909095
2211	8	-0.15073691713639192
2211	9	0.07260605239425644
2211	10	-0.016306316122886244
2211	11	0.0298692688231057
2211	12	0.02697866049906139
2211	13	-0.02618400430172585
2211	14	0.08574347349001954
2211	15	0.13659658830259502
2211	16	0.10014224953638262
2211	17	0.18689888093859963
2211	18	0.12815723664991197
2211	19	0.1270964586241803
2211	20	0.024963399331291214
2211	21	0.0860954956447273
2211	22	0.05322537859863985
2211	23	0.07698111212456382
2211	24	0.036748748817593935
2211	25	-0.09928208748792144
2212	1	-0.4604669624160991
2212	2	-0.6224433195233594
2212	3	-0.4489660550177419
2212	4	-0.5763603407066569
2212	5	-0.5072203711692964
2212	6	-0.42045982058825615
2212	7	-0.5616214912386965
2212	8	-0.5699208816552215
2212	9	-0.600903354659664
2212	10	-0.49643827346398695
2212	11	-0.45172213357626134
2212	12	-0.7037370748653444
2212	13	-0.513096014457112
2212	14	-0.5461962367366766
2212	15	-0.505075367628038
2212	16	-0.45734029589251435
2212	17	-0.2755708527175784
2212	18	-0.5874586295748045
2212	19	-0.5966315713404811
2212	20	-0.47637850652764513
2212	21	-0.5798345920395178
2212	22	-0.6472259719267583
2212	23	-0.5587551142402631
2212	24	-0.5018472062667669
2212	25	-0.5523095054651534
2213	1	-0.6813137635483599
2213	2	-0.7253604737340034
2213	3	-0.8281246603137262
2213	4	-0.7602001405143426
2213	5	-0.7492005141307061
2213	6	-0.7198118215459672
2213	7	-0.8952483163909406
2213	8	-0.7729168152488413
2213	9	-0.9073532235537769
2213	10	-0.7725271860381824
2213	11	-0.6329301613498544
2213	12	-0.8272005872673063
2213	13	-0.9302540868337417
2213	14	-0.922900709783697
2213	15	-0.9377170643196919
2213	16	-1.1353643236043212
2213	17	-1.020437642187403
2213	18	-0.9810041265993377
2213	19	-0.8212242548983752
2213	20	-0.9096264955294231
2213	21	-0.9031583096540459
2213	22	-1.0529591029052057
2213	23	-1.1973913813332615
2213	24	-1.188867144401776
2213	25	-1.1294302734869035
2214	1	-0.391165761810349
2214	2	-0.37296252066805025
2214	3	-0.251556743129715
2214	4	-0.4113336515168058
2214	5	-0.35503033082721913
2214	6	-0.27706047710121595
2214	7	-0.0023255560470047953
2214	8	-0.12712304810886366
2214	9	-0.227711424118759
2214	10	-0.19762765677112526
2214	11	-0.2508345165643391
2214	12	-0.08306089652505477
2214	13	-0.06344727042474574
2214	14	-0.19907821903783685
2214	15	-0.16360054276667313
2214	16	-0.1126631597296921
2214	17	0.0015041086400273684
2214	18	-0.18810470305702154
2214	19	0.012114235739183243
2214	20	-0.042986646844731524
2214	21	-0.37795391924095867
2214	22	-0.2369549564326121
2214	23	-0.3521962482812597
2214	24	-0.3502952793013847
2214	25	-0.1748145506713712
2215	1	-1.165586687157397
2215	2	-1.2003477656645813
2215	3	-1.1882780715898846
2215	4	-1.4253001510035403
2215	5	-1.360200457650393
2215	6	-1.216663716729272
2215	7	-1.2466970363050933
2215	8	-1.4150858204692298
2215	9	-1.4268577567709215
2215	10	-1.3664539391466164
2215	11	-1.4064468136274897
2215	12	-1.5447358403932228
2215	13	-1.4749757330464632
2215	14	-1.7892804026921922
2215	15	-1.5858380182452052
2215	16	-1.5971473262588987
2215	17	-1.6152371505397625
2215	18	-1.751437222984329
2215	19	-1.8858457964880557
2215	20	-1.6515034168684934
2215	21	-2.091428694338738
2215	22	-2.0794443333045223
2215	23	-2.2319398481618435
2215	24	-2.139749447507384
2215	25	-2.2732425867320742
2216	1	-0.6491680951035619
2216	2	-0.6549223353314026
2216	3	-0.8150897016071426
2216	4	-0.5826477922024438
2216	5	-0.7475457858146584
2216	6	-0.7225668974484449
2216	7	-0.7047975010866665
2216	8	-0.6461253614998981
2216	9	-0.7718122769743871
2216	10	-0.7269828926071753
2216	11	-0.6192361159411206
2216	12	-0.8094211391799675
2216	13	-0.8054866369290039
2216	14	-0.8553373112521996
2216	15	-0.903620312261566
2216	16	-1.0850493642994223
2216	17	-1.2812527407886378
2216	18	-1.324673435222067
2216	19	-1.1211535783669078
2216	20	-1.0902050334227056
2216	21	-1.2056332970799843
2216	22	-1.074526663499114
2216	23	-0.8728580240671349
2216	24	-1.159926226616534
2216	25	-1.081590862532785
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20998.0
3	1.0
4	195.0
5	1143.0
6	1762.0
7	1846.0
8	1547.0
9	1415.0
10	1351.0
11	1906.0
12	2044.0
13	1730.0
14	920.0
15	993.0
16	1037.0
17	1056.0
18	1212.0
19	1528.0
20	1919.0
21	2468.0
22	3045.0
23	3441.0
24	4037.0
25	4709.0
26	5244.0
27	5754.0
28	6702.0
29	8002.0
30	10001.0
31	12848.0
32	16871.0
33	22255.0
34	30968.0
35	43963.0
36	66711.0
37	124282.0
38	329346.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.39418037425514	15.642310913243298	11.954872158842688	25.008636553658874
2	15.382385389069182	20.536823937102564	37.430175845472874	26.650614828355383
3	20.011516608479607	26.89315996676559	26.88926740678997	26.206056017964833
4	13.437937135692287	14.639028280078293	33.99878642405888	37.92424816017054
5	16.87368794059693	34.06898143922514	30.49548195196856	18.56184866820937
6	35.82771311298221	32.837706654707894	14.863026849503411	16.47155338280649
7	28.916311390110444	30.980697572929607	20.975125046157977	19.127865990801972
8	28.004243290967562	31.119302274054828	20.95406906090331	19.9223853740743
9	29.54792004554096	15.948868457514795	16.665816353930595	37.83739514301365
10	16.54472963539709	24.569081243623476	28.315389572034583	30.570799548944855
11	35.657263999351855	23.40872571127645	19.155110252913296	21.778900036458403
12	27.461463620010985	23.19118875438059	25.29454708367688	24.05280054193155
13	30.693185512756	18.859400020775464	25.314903427816727	25.132511038651806
14	28.148483879713975	19.83332726318672	22.551538917058867	29.46664994004044
15	30.265509886637897	22.491915715686815	22.83768160128863	24.404892796386658
16	27.16367584481445	23.50118351574986	25.771510651641748	23.56362998779394
17	25.72549633188515	26.30805150872214	24.302626556369194	23.663825603023515
18	27.658469606314505	20.593765342512317	29.210524896008117	22.537240155165065
19	26.79312428838921	26.905363184562837	22.03996891845952	24.261543608588436
20	24.937041298480224	24.80633539216493	24.799591021354026	25.457032288000818
21	26.267361298468643	26.687431876800883	21.941335808251406	25.10387101647907
22	24.99028880028918	25.25775142565807	20.851699190193845	28.900260583858906
23	24.9713025473626	22.936245118735286	22.220663514780366	29.871788819121743
24	23.31311758176995	26.120531192810258	21.744542488816602	28.821808736603188
25	26.190036265845233	24.738323517234033	21.925933518461324	27.14570669845941
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	198.0
1	198.0
2	268.0
3	338.0
4	338.0
5	338.0
6	760.0
7	1182.0
8	1182.0
9	1182.0
10	1350.0
11	1518.0
12	1518.0
13	1518.0
14	1860.5
15	2203.0
16	2203.0
17	2203.0
18	3381.0
19	4559.0
20	4559.0
21	4559.0
22	6359.0
23	8159.0
24	8159.0
25	8159.0
26	12149.5
27	16140.0
28	16140.0
29	16140.0
30	19278.0
31	22416.0
32	22416.0
33	22416.0
34	28921.0
35	35426.0
36	35426.0
37	35426.0
38	39161.5
39	42897.0
40	42897.0
41	42897.0
42	50216.0
43	57535.0
44	57535.0
45	57535.0
46	82263.0
47	106991.0
48	106991.0
49	106991.0
50	137169.5
51	167348.0
52	167348.0
53	167348.0
54	121379.5
55	75411.0
56	75411.0
57	75411.0
58	66908.0
59	58405.0
60	58405.0
61	58405.0
62	54129.0
63	49853.0
64	49853.0
65	49853.0
66	43376.0
67	36899.0
68	36899.0
69	36899.0
70	29201.0
71	21503.0
72	21503.0
73	21503.0
74	18443.0
75	15383.0
76	15383.0
77	15383.0
78	13389.5
79	11396.0
80	11396.0
81	11396.0
82	8532.5
83	5669.0
84	5669.0
85	5669.0
86	4178.5
87	2688.0
88	2688.0
89	2688.0
90	1862.5
91	1037.0
92	1037.0
93	1037.0
94	556.5
95	76.0
96	76.0
97	76.0
98	48.0
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17698758805769876
2	0.022006038242200605
3	0.03206977524320698
4	0.0461590070446159
5	0.03193559208319356
6	0.06293190204629319
7	0.07044615900704461
8	0.07312982220731298
9	0.057162026165716195
10	0.04428044280442805
11	0.6279771888627977
12	0.5631667225763167
13	0.5355249916135525
14	0.5255954377725596
15	0.536330090573633
16	0.5119087554511909
17	0.5180811808118082
18	0.5160684334116069
19	0.5332438778933244
20	0.5221066756122107
21	0.5294867494129486
22	0.5149949681314995
23	0.5226434082522644
24	0.5030526668903053
25	0.5073465280107347
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	745250.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.284243023282485
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.19873607173186	32.13854623998236
2	7.653426114133016	5.707043984001436
3	1.9350358946624786	2.164390456661121
4	0.9079455959280561	1.3540825700200272
5	0.5483400705679485	1.022222222522963
6	0.3746845916388952	0.8381898823046355
7	0.2623685306730157	0.6847548441491984
8	0.20855331313819875	0.6220601928284265
9	0.17861235280152357	0.5993483731931047
>10	1.2668211578389112	9.625310388954981
>50	0.21013343775112733	5.402818147025664
>100	0.21447354305539346	17.398757328256604
>500	0.027125658902452797	6.912440995185132
>1k	0.011211939013013823	7.068460078531716
>5k	0.0018083772601635202	4.703496652661037
>10k+	7.233509040654079E-4	3.7580776437216294
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	17254	2.3151962428715196	No Hit
CTTCCGTACTCTGCGTTGATACCAC	10615	1.4243542435424354	No Hit
GAGTACGGAAGCAGTGGTATCAACG	8748	1.1738342837973834	No Hit
TCCGTACTCTGCGTTGATACCACTG	8387	1.1253941630325395	No Hit
GTACATGGGAAGCAGTGGTATCAAC	6478	0.8692385105669239	No Hit
GCTTCCGTACTCTGCGTTGATACCA	5695	0.7641730962764173	No Hit
CCCATGTACTCTGCGTTGATACCAC	5572	0.7476685675947669	No Hit
GCGTTGATACCACTGCTTCCGTACT	4267	0.5725595437772559	No Hit
GTATCAACGCAGAGTACGGAAGCAG	3678	0.4935256625293526	No Hit
ACTCTGCGTTGATACCACTGCTTCC	2752	0.3692720563569272	No Hit
TATCAACGCAGAGTACGGAAGCAGT	2663	0.357329755115733	No Hit
GAGTACATGGGAAGCAGTGGTATCA	2102	0.2820530023482053	No Hit
GTACATGGAAGCAGTGGTATCAACG	2046	0.2745387453874539	No Hit
CAGTGGTATCAACGCAGAGTACGGA	2003	0.2687688695068769	No Hit
CATGTACTCTGCGTTGATACCACTG	1886	0.25306943978530694	No Hit
GGTATCAACGCAGAGTACGGAAGCA	1880	0.2522643408252264	No Hit
ATACCACTGCTTCCGTACTCTGCGT	1855	0.248909761824891	No Hit
TCCATGTACTCTGCGTTGATACCAC	1840	0.2468970144246897	No Hit
ACGCAGAGTACGGAAGCAGTGGTAT	1806	0.24233478698423347	No Hit
GTATCAACGCAGAGTACATGGGAAG	1728	0.23186850050318686	No Hit
GCGTTGATACCACTGCTTCCCATGT	1576	0.21147266018114724	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1402	0.1881247903388125	No Hit
GTACTCTGCGTTGATACCACTGCTT	1331	0.17859778597785977	No Hit
AAGCAGTGGTATCAACGCAGAGTAC	1331	0.17859778597785977	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1316	0.1765850385776585	No Hit
GTGGTATCAACGCAGAGTACGGAAG	1313	0.17618248909761824	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1268	0.17014424689701443	No Hit
TATCAACGCAGAGTACATGGGAAGC	1267	0.170010063737001	No Hit
GAAGCAGTGGTATCAACGCAGAGTA	1230	0.16504528681650452	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1194	0.16021469305602146	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1193	0.16008050989600806	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1171	0.15712848037571284	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1153	0.1547131834954713	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	1088	0.14599127809459914	No Hit
GTTGATACCACTGCTTCCGTACTCT	1060	0.14223414961422343	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1007	0.13512244213351224	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1007	0.13512244213351224	No Hit
CTTTAATATACGCTATTGGAGCTGG	1005	0.1348540758134854	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	980	0.13149949681314996	No Hit
GATACCACTGCTTCCGTACTCTGCG	977	0.1310969473331097	No Hit
GAATAACGCCGCCGCATCGCCAGTC	977	0.1310969473331097	No Hit
ATCAACGCAGAGTACGGAAGCAGTG	967	0.12975511573297552	No Hit
CCACTGCTTCCGTACTCTGCGTTGA	956	0.1282791009728279	No Hit
GAACTACGACGGTATCTGATCGTCT	940	0.1261321704126132	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	933	0.1251928882925193	No Hit
GCTTCCCATGTACTCTGCGTTGATA	924	0.12398523985239851	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	898	0.12049647769204964	No Hit
GGTATCAACGCAGAGTACATGGGAA	894	0.11995974505199598	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	872	0.11700771553170078	No Hit
ATCAGATACCGTCGTAGTTCCGACC	854	0.11459241865145924	No Hit
CTGCTTCCGTACTCTGCGTTGATAC	852	0.11432405233143239	No Hit
GTACAAAGGGCAGGGACTTAATCAA	832	0.11164038913116403	No Hit
CGGAAGCAGTGGTATCAACGCAGAG	829	0.1112378396511238	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	821	0.11016437437101645	No Hit
TCGTAGTTCCGACCATAAACGATGC	816	0.10949345857094933	No Hit
CAGTGGTATCAACGCAGAGTACATG	806	0.10815162697081517	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	787	0.1056021469305602	No Hit
GAATAATGGAATAGGACCGCGGTTC	780	0.10466286481046627	No Hit
GAATAATTGCAATCCCCGATCCCCA	777	0.10426031533042604	No Hit
CTCTAGATAACCTCGGGCCGATCGC	775	0.1039919490103992	No Hit
TATCAACGCAGAGTACTTTTTTTTT	774	0.10385776585038577	No Hit
GCTTTGAACACTCTAATTTTTTCAA	762	0.10224756793022476	No Hit
GAGTACATGGAAGCAGTGGTATCAA	747	0.10023482053002349	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.3418316001341833E-4	0.0	0.0	0.0
5	0.0	1.3418316001341833E-4	0.0	0.0	0.0
6	0.0	1.3418316001341833E-4	0.0	1.3418316001341833E-4	0.0
7	0.0	1.3418316001341833E-4	0.0	2.6836632002683665E-4	0.0
8	0.0	1.3418316001341833E-4	0.0	2.6836632002683665E-4	0.0
9	0.0	1.3418316001341833E-4	0.0	2.6836632002683665E-4	0.0
10	0.0	1.3418316001341833E-4	0.0	5.367326400536733E-4	0.0
11	0.0	1.3418316001341833E-4	0.0	5.367326400536733E-4	0.0
12	0.0	1.3418316001341833E-4	0.0	6.709158000670916E-4	2.6836632002683665E-4
13	0.0	1.3418316001341833E-4	0.0	8.050989600805099E-4	2.6836632002683665E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCTTA	35	0.0021613203	16.294096	17
GAGGTTA	70	4.3710133E-7	14.945324	5
TTCGGAA	105	8.731149E-11	14.48364	18
CGCTTAC	40	0.0052576843	14.257334	18
AGAATTT	190	0.0	14.007205	17
CGCCAGT	130	1.8189894E-12	13.891761	18
TGTACCG	55	1.939437E-4	13.833688	5
TAGAGTG	70	7.1667837E-6	13.5866585	5
AAATCGC	70	7.2118764E-6	13.578413	14
GCTCGTA	85	2.65607E-7	13.426815	9
GGCGGGA	50	0.0014917023	13.307743	12
TCGCAGT	50	0.0014924606	13.306845	16
GCGCGCT	100	9.966243E-9	13.306845	15
CGCGCTA	100	9.966243E-9	13.306845	16
GGTTTTC	115	3.7289283E-10	13.224193	14
GTTTTCG	115	3.7289283E-10	13.224193	15
TTTTCGG	115	3.7289283E-10	13.224193	16
AAGCTCG	90	5.3193435E-7	12.6808815	7
AAGAATT	225	0.0	12.673185	16
CGCAGTT	60	4.0698433E-4	12.673185	17
>>END_MODULE