FastQCFastQC Report
Thu 2 Feb 2017
SRR4050278_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050278_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences745250
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA141521.8989600805098958No Hit
CTTCCGTACTCTGCGTTGATACCAC104301.399530358939953No Hit
TCCGTACTCTGCGTTGATACCACTG89341.1987923515598793No Hit
GAGTACGGAAGCAGTGGTATCAACG82921.1126467628312646No Hit
CCCATGTACTCTGCGTTGATACCAC62480.8383763837638376No Hit
GCTTCCGTACTCTGCGTTGATACCA56590.7593425025159343No Hit
GTACATGGGAAGCAGTGGTATCAAC52010.6978866152297887No Hit
GCGTTGATACCACTGCTTCCGTACT42760.5737671922173767No Hit
GTATCAACGCAGAGTACGGAAGCAG35760.47983898020798393No Hit
ACTCTGCGTTGATACCACTGCTTCC29830.4002683663200268No Hit
TATCAACGCAGAGTACGGAAGCAGT29500.3958403220395841No Hit
CAGTGGTATCAACGCAGAGTACGGA21720.29144582354914456No Hit
CATGTACTCTGCGTTGATACCACTG21610.289969808788997No Hit
ACGCAGAGTACGGAAGCAGTGGTAT20970.28138208654813823No Hit
GTATCAACGCAGAGTACTTTTTTTT20330.27279436430727944No Hit
ATACCACTGCTTCCGTACTCTGCGT19930.2674270379067427No Hit
TCCATGTACTCTGCGTTGATACCAC19750.26501174102650116No Hit
GAGTACATGGGAAGCAGTGGTATCA19280.2587051325058705No Hit
GGTATCAACGCAGAGTACGGAAGCA19250.25830258302583026No Hit
GTACATGGAAGCAGTGGTATCAACG16710.224220060382422No Hit
GCGTTGATACCACTGCTTCCCATGT15950.2140221402214022No Hit
AAGCAGTGGTATCAACGCAGAGTAC15610.209459912780946No Hit
GTATCAACGCAGAGTACATGGGAAG15050.20194565582019458No Hit
TATCAACGCAGAGTACTTTTTTTTT14870.19953035893995305No Hit
GTGGTATCAACGCAGAGTACGGAAG13790.18503857765850387No Hit
GAAGCAGTGGTATCAACGCAGAGTA13750.18450184501845018No Hit
TATCAACGCAGAGTACATGGGAAGC12690.17027843005702784No Hit
GAATAGGACCGCGGTTCTATTTTGT12470.16732640053673264No Hit
GATTAAGAGGGACGGCCGGGGGCAT12470.16732640053673264No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG11760.15779939617577995No Hit
CTATTGGAGCTGGAATTACCGCGGC11710.15712848037571284No Hit
ACGCAGAGTACATGGGAAGCAGTGG11610.15578664877557866No Hit
GTACTCTGCGTTGATACCACTGCTT11210.15041932237504194No Hit
ATCAACGCAGAGTACGGAAGCAGTG11070.14854075813485407No Hit
GATACCACTGCTTCCGTACTCTGCG10950.14693056021469306No Hit
CTCTTAATCATGGCCTCAGTTCCGA10700.14357598121435758No Hit
CCACTGCTTCCGTACTCTGCGTTGA10370.1391479369339148No Hit
GAATAACGCCGCCGCATCGCCAGTC10340.13874538745387452No Hit
GCTTCCCATGTACTCTGCGTTGATA10290.13807447165380746No Hit
GTATCTGATCGTCTTCGAACCTCCG10270.1378061053337806No Hit
GTTGATACCACTGCTTCCGTACTCT10230.13726937269372694No Hit
GTCCTATTCCATTATTCCTAGCTGC10230.13726937269372694No Hit
GTACTTTTTTTTTTTTTTTTTTTTT10150.1361959074136196No Hit
CTTTAATATACGCTATTGGAGCTGG10070.13512244213351224No Hit
GAACTACGACGGTATCTGATCGTCT9710.13029184837302918No Hit
ATACAGGACTCTTTCGAGGCCCTGT9690.13002348205300235No Hit
GCGCAAGACGGACCAGAGCGAAAGC9660.1296209325729621No Hit
ATATTAAAGTTGCTGCAGTTAAAAA9380.12586380409258638No Hit
ATCAGATACCGTCGTAGTTCCGACC9360.12559543777255955No Hit
CGGAAGCAGTGGTATCAACGCAGAG9180.123180140892318No Hit
GTCTTGCGCCGGTCCAAGAATTTCA9130.12250922509225093No Hit
CTGCTTCCGTACTCTGCGTTGATAC8600.11539751761153974No Hit
GGTATCAACGCAGAGTACATGGGAA8570.11499496813149951No Hit
GGTATCAACGCAGAGTACTTTTTTT8320.11164038913116403No Hit
CTCTAGATAACCTCGGGCCGATCGC8170.10962764173096276No Hit
AAAAAGTACTCTGCGTTGATACCAC8120.10895672593089567No Hit
TCGTAGTTCCGACCATAAACGATGC8090.10855417645085541No Hit
CCATTATTCCTAGCTGCGGTATCCA7940.10654142905065414No Hit
GAATAATGGAATAGGACCGCGGTTC7910.1061388795706139No Hit
CAGTGGTATCAACGCAGAGTACATG7810.1047970479704797No Hit
GCTTTGAACACTCTAATTTTTTCAA7760.10412613217041261No Hit
GTCCTGTATTGTTATTTTTCGTCAC7580.10171083529017108No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTGT250.006039784318.9942286
GCGTTCC453.554338E-516.8780929
CCGCGCA350.002178057616.2753039
ATCGCCA1750.015.18722516
CGCCAGT1750.015.18722518
TTAAACT456.676138E-414.8011064
CCGCGCT456.7893235E-414.7683319
GCCGCCT2000.014.71262518
GCGTTAT1006.220944E-1014.3023151
GTATGGA400.00514894414.3023151
GAGCGTT400.005291045614.2437587
AGCGTTC400.005298118614.24089058
GCGTAAC602.5818676E-514.23993511
CATCGCC1800.014.23802415
TCGCCAG2050.013.89075617
CGAACGA551.9720115E-413.80656916
GTTATCA706.981001E-613.6212521
CGCGTAA707.3035226E-613.56184310
CCGCCTG2200.013.39398519
CCGACCA1351.8189894E-1213.3618239
Produced by FastQC (version 0.11.5)