FastQCFastQC Report
Thu 2 Feb 2017
SRR4050277_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050277_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9420167
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA129610.13758779435651194No Hit
GTCCTAAAGTGTGTATTTCTCATTT106150.11268377726212284No Hit
CTTTAGGACGTGAAATATGGCGAGG101680.10793863845513565No Hit
CTGTAGGACGTGGAATATGGCAAGA100890.10710001213354287No Hit
GTCCTACAGTGGACATTTCTAAATT96730.10268395454135792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41800.015.3757331
GTATCAA141550.013.5478971
AACCGCG6700.011.4969787
TAGATCG3050.010.9129965
CGCTTAC3050.010.59932618
CGTCGTA10450.010.28414310
CTCGACG2900.010.16461213
TCGACGC2900.010.1643414
AGGACCT47050.010.1465855
CGACCAT10100.010.075541510
ACGGTAT11550.010.0457849
TGGCGAG68200.010.03800418
AGGACGT64750.09.9872275
ACGCTTA3150.09.96093917
GGACGTG64400.09.8939436
CCGACCA11350.09.8876289
GACGTGA36800.09.8716347
TGTAGGA68550.09.7344732
TTAGGAC47200.09.6858093
GTCCTAA38550.09.674181
Produced by FastQC (version 0.11.5)