FastQCFastQC Report
Thu 2 Feb 2017
SRR4050277_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050277_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9420167
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT136910.14533712618895184No Hit
GTACAAAGGGCAGGGACTTAATCAA123660.1312715581369205No Hit
CTTTAGGACGTGAAATATGGCGAGG101550.1078006366553799No Hit
GTCCTAAAGTGTGTATTTCTCATTT101390.1076307882864497No Hit
CTGTAGGACGTGGAATATGGCAAGA99320.10543337501341538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45800.015.4605241
GTATCAA143550.013.147291
AACCGCG6900.010.8727957
TCGCGTA5700.010.4950659
GCGTTAT4950.010.3825811
AGAACCG8150.010.3759675
CGACCAT12500.010.25490610
CGTCGTA10000.010.25490510
GGTAGCG3650.010.14564411
CTCGACG3500.010.03499213
CGGTTCT14800.010.00779712
ACGGTAT11150.09.9639249
AGGACGT61000.09.8598455
ATACCGT11500.09.8301986
CGCGTAA6200.09.80158110
GGACGTG61050.09.7565226
CCGTCCA4200.09.721629
CGAGCCG14850.09.65226615
TGTAGGA70350.09.5907012
TACCGTC11350.09.5383287
Produced by FastQC (version 0.11.5)