FastQCFastQC Report
Thu 2 Feb 2017
SRR4050276_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050276_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669328
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA44530.2667540471375308No Hit
GTATCTGATCGTCTTCGAACCTCCG31210.18696145994076657No Hit
GAATAGGACCGCGGTTCTATTTTGT29700.17791590388467696No Hit
CTATTGGAGCTGGAATTACCGCGGC29210.17498059099230348No Hit
GATTAAGAGGGACGGCCGGGGGCAT28800.17252451285786854No Hit
GTCCTATTCCATTATTCCTAGCTGC28160.16869063479436036No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG27330.16371857418074817No Hit
CTCTTAATCATGGCCTCAGTTCCGA26500.15874651356713598No Hit
CTTCCGTACTCTGCGTTGATACCAC25920.1552720615720817No Hit
CTTTAATATACGCTATTGGAGCTGG25260.15131837481908889No Hit
GAACTACGACGGTATCTGATCGTCT24570.14718497503186911No Hit
GTACAAAGGGCAGGGACTTAATCAA23750.14227281876299924No Hit
GAATAACGCCGCCGCATCGCCAGTC23730.1421530100735146No Hit
GTCTTGCGCCGGTCCAAGAATTTCA23500.14077521014444136No Hit
ATATTAAAGTTGCTGCAGTTAAAAA22750.13628238428876768No Hit
ATACAGGACTCTTTCGAGGCCCTGT22380.13406592353330204No Hit
GTCCTGTATTGTTATTTTTCGTCAC21560.12915376726443215No Hit
GAGTACGGAAGCAGTGGTATCAACG21140.1266377847852549No Hit
ATCAGATACCGTCGTAGTTCCGACC20830.12478075009824312No Hit
GCTTTGAACACTCTAATTTTTTCAA20690.12394208927185071No Hit
CTCTAGATAACCTCGGGCCGATCGC20500.12280390672174671No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC20230.12118648941370419No Hit
GAATAATGGAATAGGACCGCGGTTC19400.11621442880009202No Hit
GCGCAAGACGGACCAGAGCGAAAGC19300.11561538535266885No Hit
GAATAATTGCAATCCCCGATCCCCA19290.11555548100792654No Hit
CCATTATTCCTAGCTGCGGTATCCA19260.11537576797369958No Hit
TCCGTACTCTGCGTTGATACCACTG19070.1142375854235956No Hit
GGGTAGGCACACGCTGAGCCAGTCA18860.11297959418400698No Hit
TCGTAGTTCCGACCATAAACGATGC18380.11010418563637585No Hit
CTCCAATAGCGTATATTAAAGTTGC18290.109565046533695No Hit
CTTTAAATCCTTTAACGAGGATCCA17900.10722877708874469No Hit
CTCCCGACCCGGGGAGGTAGTGACG17860.10698915970977543No Hit
AGATAACCTCGGGCCGATCGCACGC16970.10165767302770935No Hit
GTGCATGGCCGTTCTTAGTTGGTGG16950.10153786433822472No Hit
GTTCTATTTTGTTGGTTTTCGGAAC16710.10010016006440917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAC753.261448E-916.4749418
TAGATCG651.8850915E-716.098095
TCGACGC851.702756E-814.53846314
ACGCTTA851.7054845E-814.53671217
TAATACT1006.9849193E-1014.1968784
CTCGACG903.6083293E-813.73118413
AACCGCG2750.013.4896537
CGTCGTA3450.012.95828710
ATTACAC751.5366082E-512.6194483
ATCGCCA4800.012.47501416
GACGCTT1001.4332363E-712.35620616
TTACCCT1001.5145451E-712.3039624
TCGCCAG5000.012.16610917
CGACGCT1103.7811333E-812.09844115
CCGACCA3650.011.9876439
CCGTCGT3750.011.9216249
CGTCAAT2950.011.92124119
CGCCAGT5050.011.8574418
CGCGCTA1850.011.81674516
ATACCGT4050.011.7438366
Produced by FastQC (version 0.11.5)