##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050276_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1669328
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.434391563551323	31.0	31.0	34.0	27.0	34.0
2	30.591379285556823	31.0	31.0	34.0	27.0	34.0
3	30.665309633577102	31.0	31.0	34.0	27.0	34.0
4	34.30745785130304	37.0	35.0	37.0	30.0	37.0
5	34.28508178141144	37.0	35.0	37.0	30.0	37.0
6	34.27634053942664	37.0	35.0	37.0	30.0	37.0
7	34.1958219115716	37.0	35.0	37.0	30.0	37.0
8	34.13333149626676	37.0	35.0	37.0	30.0	37.0
9	35.68771445755418	39.0	37.0	39.0	30.0	39.0
10	35.54808761369845	39.0	37.0	39.0	30.0	39.0
11	35.427685871200865	39.0	37.0	39.0	30.0	39.0
12	35.33790243738798	39.0	35.0	39.0	30.0	39.0
13	35.39088723126911	39.0	37.0	39.0	30.0	39.0
14	36.543123939693096	40.0	37.0	41.0	30.0	41.0
15	36.50288918654692	40.0	37.0	41.0	30.0	41.0
16	36.49493868191272	40.0	37.0	41.0	30.0	41.0
17	36.43239075843693	40.0	37.0	41.0	30.0	41.0
18	36.419673066048134	40.0	37.0	41.0	30.0	41.0
19	36.41295239761149	40.0	37.0	41.0	30.0	41.0
20	36.34095516279605	40.0	37.0	41.0	29.0	41.0
21	36.25406930213835	40.0	37.0	41.0	29.0	41.0
22	36.16366885357461	40.0	37.0	41.0	29.0	41.0
23	36.0829573337295	40.0	37.0	41.0	29.0	41.0
24	36.00515416982163	40.0	37.0	41.0	28.0	41.0
25	35.81271385851073	40.0	36.0	41.0	27.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.8313037990891097
1101	2	0.8202481713271368
1101	3	0.661817817488842
1101	4	0.71490454165626
1101	5	0.7610437483127086
1101	6	0.8491197671083839
1101	7	0.8534561668766472
1101	8	0.9610720551127869
1101	9	1.0314692484638357
1101	10	1.0105835517825668
1101	11	0.8968065332253801
1101	12	1.0353527986398277
1101	13	1.0927373851161448
1101	14	1.2731099680277396
1101	15	1.2744654500996262
1101	16	1.3304164127763585
1101	17	1.3215341517295656
1101	18	1.2473916612518394
1101	19	1.2498013052534986
1101	20	1.198641570729606
1101	21	1.2659531904609551
1101	22	1.2720058637706089
1101	23	1.3865119763807314
1101	24	1.248374524578999
1101	25	1.3629406851703365
1102	1	0.48962389126134553
1102	2	0.4767198318031305
1102	3	0.4102897531058467
1102	4	0.43580614435263954
1102	5	0.5175348406225169
1102	6	0.41252790440375264
1102	7	0.4564304411922322
1102	8	0.41535686045052245
1102	9	0.5251325790457599
1102	10	0.4843758547128161
1102	11	0.4940718035514209
1102	12	0.6519377828192177
1102	13	0.7000081582623849
1102	14	0.720751493684638
1102	15	0.7455668739543526
1102	16	0.6359704779847775
1102	17	0.6826630896852635
1102	18	0.6536141471508614
1102	19	0.7236372868190486
1102	20	0.6958911924107198
1102	21	0.6642311516660726
1102	22	0.7646852213163484
1102	23	0.7819747291665351
1102	24	0.8812304882603854
1102	25	0.8792431339253284
1103	1	0.5670242424388618
1103	2	0.6588237049888797
1103	3	0.5878317747959017
1103	4	0.6233753955150476
1103	5	0.7338824182634482
1103	6	0.7029600790952486
1103	7	0.6637517333791081
1103	8	0.7578228761308523
1103	9	0.850046834670735
1103	10	0.7753967701733728
1103	11	0.8328430685291579
1103	12	0.8306422075068198
1103	13	0.822747647841922
1103	14	0.9205340238569732
1103	15	1.0238271085561124
1103	16	0.9879246229569816
1103	17	0.8955427724192191
1103	18	1.1044626793142385
1103	19	1.075562799506372
1103	20	1.0007064311565372
1103	21	1.0049228127926426
1103	22	0.8934529081055871
1103	23	0.897174948466116
1103	24	0.9754352799156152
1103	25	1.025342163002854
1104	1	0.2939354494691315
1104	2	0.34742786730976505
1104	3	0.39936667742369636
1104	4	0.4990834233066366
1104	5	0.5220684497242232
1104	6	0.506935999464524
1104	7	0.5815289138907644
1104	8	0.538044096372289
1104	9	0.6538348836429932
1104	10	0.605009447548035
1104	11	0.6368350348540446
1104	12	0.549447532624626
1104	13	0.6478527488512142
1104	14	0.6853317378431569
1104	15	0.6854874370703072
1104	16	0.6755874225483467
1104	17	0.607273836854425
1104	18	0.6538588165178538
1104	19	0.7183938676856343
1104	20	0.6503978139435063
1104	21	0.7435509103306615
1104	22	0.6886195988411856
1104	23	0.6018417809409939
1104	24	0.75306507832493
1104	25	0.873687156397267
1105	1	0.23332046575577436
1105	2	0.3482148379938046
1105	3	0.4327178174888431
1105	4	0.3951212083229265
1105	5	0.2623270816460419
1105	6	0.32480310044172
1105	7	0.2791895002099807
1105	8	0.3533887217794529
1105	9	0.381619248463835
1105	10	0.43663355178256325
1105	11	0.6058565332253778
1105	12	0.5943361319731579
1105	13	0.6444540517828088
1105	14	0.7008933013610772
1105	15	0.6977154500996221
1105	16	0.7001164127763602
1105	17	0.6729174850628965
1105	18	0.72310832791851
1105	19	0.6828179719201657
1105	20	0.69320823739627
1105	21	0.6440531904609514
1105	22	0.7219225304372756
1105	23	0.7690619763807334
1105	24	0.8195745245790036
1105	25	0.8937073518370084
1106	1	0.45862809627786305
1106	2	0.6119851191183017
1106	3	0.5706230383723749
1106	4	0.5639356661542507
1106	5	0.5819624230115039
1106	6	0.45991795987946915
1106	7	0.5615686166758422
1106	8	0.5238079988878894
1106	9	0.6708818990662451
1106	10	0.7182994152363804
1106	11	0.7769119549121299
1106	12	0.7176068147040837
1106	13	0.7291881883289975
1106	14	0.8965286427265369
1106	15	0.9012174581317538
1106	16	0.912836091491215
1106	17	0.8543504167898064
1106	18	0.865689854022925
1106	19	0.8971456827635365
1106	20	0.833812253460529
1106	21	0.7379712627501078
1106	22	0.8419305625657927
1106	23	0.8186153900353546
1106	24	0.753314283615147
1106	25	0.788051126937404
1107	1	0.4157668472031979
1107	2	0.4925655776979241
1107	3	0.4730463260854023
1107	4	0.46359989684751923
1107	5	0.5171894566960233
1107	6	0.47248658704707935
1107	7	0.4301241663435249
1107	8	0.2192286258178413
1107	9	0.21563508213036897
1107	10	0.3766436277521805
1107	11	0.5538402197507679
1107	12	0.5407598025049438
1107	13	0.6452142277124366
1107	14	0.7103642329884252
1107	15	0.7124007759692788
1107	16	0.7375945415248566
1107	17	0.6940383167302286
1107	18	0.6614518705014731
1107	19	0.6551619943112073
1107	20	0.7659349866965499
1107	21	0.7927994519563555
1107	22	0.5847845856152034
1107	23	0.7582907648653361
1107	24	0.7864273034674483
1107	25	0.7866335413611978
1108	1	0.27947173677650383
1108	2	0.26734209649986695
1108	3	0.2239228233550925
1108	4	0.27314874058729544
1108	5	0.2550590331517171
1108	6	0.20447255683593113
1108	7	0.14262055222405934
1108	8	0.2961103486859855
1108	9	0.2591300814712696
1108	10	0.3384780570621899
1108	11	0.27293138656914095
1108	12	0.11108200604433449
1108	13	0.06892350817702209
1108	14	0.17650831895982577
1108	15	0.23682221584072494
1108	16	0.2307263462921938
1108	17	0.23956683977544913
1108	18	0.3161628449306306
1108	19	0.28565207438398943
1108	20	0.2476498486594707
1108	21	0.32759671803232493
1108	22	0.14743132981154616
1108	23	0.18614778005691335
1108	24	0.4044910674026241
1108	25	0.344578998297699
1109	1	-0.017223872056124634
1109	2	-0.009471918244198463
1109	3	0.04019113802626961
1109	4	-0.07787456902832446
1109	5	0.034739365715140025
1109	6	-0.01860496097862807
1109	7	0.05700063264760047
1109	8	-0.1149844068193957
1109	9	-0.008504359981458265
1109	10	0.025250058500418504
1109	11	0.12917937391635803
1109	12	0.10089582487142934
1109	13	0.10688245869260271
1109	14	0.09940155472000356
1109	15	-0.01228838867196913
1109	16	0.11007572179747172
1109	17	0.13335356951586874
1109	18	0.09727569835996519
1109	19	0.2000548241274558
1109	20	0.23616716254022663
1109	21	0.09338601195807428
1109	22	0.16986494886337766
1109	23	0.20953260977804433
1109	24	0.09073536910874935
1109	25	0.13358527890034821
1110	1	-0.019911012171288434
1110	2	0.033138446439103575
1110	3	-0.09263035909465245
1110	4	0.026540018303734314
1110	5	0.08272401062876611
1110	6	-0.08271244658322985
1110	7	-0.009793993072165108
1110	8	0.03088891371803726
1110	9	0.1304591716884076
1110	10	-0.03770579562626608
1110	11	0.04511027602768536
1110	12	0.17460023945876202
1110	13	-0.0013709002325441588
1110	14	0.13970865644744634
1110	15	0.1715887706370509
1110	16	0.1711121901276087
1110	17	0.2657911894774827
1110	18	0.3141662933695599
1110	19	0.1973676840122991
1110	20	0.18741476330797724
1110	21	0.299911923666329
1110	22	0.17485535193439716
1110	23	0.09590497062257697
1110	24	0.2450539871509747
1110	25	0.22917069156829228
1111	1	-0.18663757301275297
1111	2	-0.18284665953565238
1111	3	-0.06638153636900768
1111	4	-0.06677875366871433
1111	5	-0.04561104074088007
1111	6	-0.02993334957728422
1111	7	-0.029105596711339388
1111	8	-0.08827087533190792
1111	9	-0.1355067112472952
1111	10	-0.06794980055494904
1111	11	-0.015639779963521505
1111	12	-0.16669252633166565
1111	13	-0.18866749895835255
1111	14	-0.22234090616458246
1111	15	-0.12654604742983366
1111	16	-0.034206354232381386
1111	17	-0.10973580266040273
1111	18	0.007575526702240154
1111	19	-0.08455397790879715
1111	20	0.07805719216631957
1111	21	0.06286216043434933
1111	22	0.04132883982533997
1111	23	-0.24917549986404453
1111	24	-0.07212338496109538
1111	25	-0.11530047788553333
1112	1	-0.08777169257133366
1112	2	-0.059636692999472274
1112	3	-0.08320272022587005
1112	4	-0.1230992547091887
1112	5	-0.12933744411945014
1112	6	-0.060395107175907015
1112	7	-0.12118660135835313
1112	8	-0.2392637054050084
1112	9	-0.24515416453093053
1112	10	-0.3181067021382731
1112	11	-0.25057304108380407
1112	12	-0.27459710178337815
1112	13	-0.3516573746548275
1112	14	-0.2870639801923218
1112	15	-0.2206624438510829
1112	16	-0.21234124219003547
1112	17	-0.37169431478773873
1112	18	-0.4516856974735788
1112	19	-0.3372838951448074
1112	20	-0.3283194698479406
1112	21	-0.43116592828437916
1112	22	-0.43371451213180734
1112	23	-0.45689261659910585
1112	24	-0.4281527345696148
1112	25	-0.4826573979762898
1113	1	-0.2681290451546836
1113	2	-0.2468379836766239
1113	3	-0.29296585444494383
1113	4	-0.2599195742203406
1113	5	-0.24071791459172687
1113	6	-0.37033941272607507
1113	7	-0.3780562484732428
1113	8	-0.4056162443605018
1113	9	-0.45170324965504705
1113	10	-0.5443665987065245
1113	11	-0.35564173615009054
1113	12	-0.47154618555882166
1113	13	-0.5589358654481913
1113	14	-0.49229164972997097
1113	15	-0.38015362439247724
1113	16	-0.2713725262755631
1113	17	-0.4564784517316838
1113	18	-0.4450791814870598
1113	19	-0.40238925155613003
1113	20	-0.45932870767521194
1113	21	-0.5360539577707968
1113	22	-0.4854348811503826
1113	23	-0.4733497617682545
1113	24	-0.5161627214706641
1113	25	-0.4960867790885004
1114	1	-0.5858822481530055
1114	2	-0.5035591537136952
1114	3	-0.5021390238228811
1114	4	-0.5631993344588295
1114	5	-0.5892174339966232
1114	6	-0.7654610533464421
1114	7	-0.5773492106833444
1114	8	-0.7170082627663419
1114	9	-0.7809662019696333
1114	10	-0.6332211033248569
1114	11	-0.6113309526396762
1114	12	-0.6899244032699627
1114	13	-0.6066263854580498
1114	14	-0.8079342900448836
1114	15	-0.6734176068170754
1114	16	-0.6733783478719673
1114	17	-0.5705994391738187
1114	18	-0.500422919725672
1114	19	-0.6694785980006799
1114	20	-0.5487409522004114
1114	21	-0.6815392709035422
1114	22	-0.6728177635695118
1114	23	-0.5960478615386009
1114	24	-0.7695259654323081
1114	25	-0.5827776839715142
1115	1	-0.3340612451586793
1115	2	-0.29798929179970557
1115	3	-0.3321571087648465
1115	4	-0.32345460288651395
1115	5	-0.28741864106782344
1115	6	-0.3637821502957408
1115	7	-0.42568837589621467
1115	8	-0.4553475614063842
1115	9	-0.45458901112318273
1115	10	-0.5321634983649233
1115	11	-0.3195393369811086
1115	12	-0.41886917776135135
1115	13	-0.5741837063292792
1115	14	-0.5935013889044143
1115	15	-0.634019063174712
1115	16	-0.8432635282265935
1115	17	-0.6996789751140966
1115	18	-0.685443884470871
1115	19	-0.8409767183453241
1115	20	-0.7383569543441411
1115	21	-0.6265763080641236
1115	22	-0.747540596406381
1115	23	-0.6701067551826867
1115	24	-0.6074366848605308
1115	25	-0.5372731791364487
1116	1	-0.6294798133789072
1116	2	-0.5684776951534403
1116	3	-0.6160101657630754
1116	4	-0.6163710456896325
1116	5	-0.7853516343344182
1116	6	-0.7751821054201073
1116	7	-0.7890374362868755
1116	8	-0.9228116969225653
1116	9	-0.7719963830783811
1116	10	-0.8036793581964972
1116	11	-0.7580530271374926
1116	12	-0.9681029468823894
1116	13	-0.8556020542883687
1116	14	-1.0704364962662822
1116	15	-1.0317165108215178
1116	16	-1.0524290163932193
1116	17	-0.953987190443037
1116	18	-1.0529645262336231
1116	19	-1.087015105539706
1116	20	-1.084323653741201
1116	21	-1.1838357139354159
1116	22	-1.3560300166667005
1116	23	-1.4429410941007745
1116	24	-1.6364056568585426
1116	25	-1.448834727716381
1201	1	0.600800782027104
1201	2	0.6045376012064558
1201	3	0.5435895611426069
1201	4	0.36901866982938003
1201	5	0.5374633280047618
1201	6	0.6836999793430962
1201	7	0.5957532954659115
1201	8	0.7261880559450802
1201	9	0.7433590736823135
1201	10	0.7917628068803637
1201	11	0.7860486264421951
1201	12	0.777214533970664
1201	13	0.7924289165352079
1201	14	0.9161993354850893
1201	15	0.9600475349935067
1201	16	0.8806900290893012
1201	17	0.9484687126950249
1201	18	0.946442327086217
1201	19	0.9247061117453867
1201	20	0.9024087367720526
1201	21	0.9418489457668215
1201	22	0.9908588600252912
1201	23	0.987981493650814
1201	24	1.0881622898723862
1201	25	1.0063936606176966
1202	1	0.5014785302719034
1202	2	0.5057051605744505
1202	3	0.46931781748884305
1202	4	0.4623373373551871
1202	5	0.5731270816460423
1202	6	0.47999342302236414
1202	7	0.4651765969841719
1202	8	0.477859689521388
1202	9	0.5777998936251265
1202	10	0.6272367775890189
1202	11	0.6356371783866734
1202	12	0.6362264545538068
1202	13	0.6992830840408786
1202	14	0.7316449142643009
1202	15	0.7341831920351041
1202	16	0.7490454450344188
1202	17	0.814262646353221
1202	18	0.7791792956604411
1202	19	0.6882018428879064
1202	20	0.8860340438478858
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2210	17	0.19889012831612973
2210	18	0.12770204326046297
2210	19	0.22057841554308766
2210	20	0.389462581204036
2210	21	0.2721315638435797
2210	22	0.2645066339492885
2210	23	0.26316400965244924
2210	24	0.2714244321575592
2210	25	0.23704265682776082
2211	1	-0.08252285768636014
2211	2	0.014444511584308373
2211	3	-0.0491539926001785
2211	4	-0.047393925208233156
2211	5	0.06333479677957143
2211	6	0.12074315978890127
2211	7	0.08392599872630058
2211	8	-0.06528427525318392
2211	9	0.02228156300390083
2211	10	0.11548340341461483
2211	11	-0.018471656685598248
2211	12	0.022042956898978616
2211	13	0.09164811706470744
2211	14	0.13880606100499193
2211	15	-0.012340929722334693
2211	16	0.04307961752413547
2211	17	-0.11330387992227031
2211	18	-0.19334152371354207
2211	19	-0.18804612303532764
2211	20	-0.1901887952446799
2211	21	-0.15349042971708826
2211	22	-0.1432377069514459
2211	23	-0.054011020651905994
2211	24	-0.09868778996106187
2211	25	-0.12192825647159822
2212	1	-0.33479870828552194
2212	2	-0.42335802336312867
2212	3	-0.3671866072899199
2212	4	-0.19697967663282867
2212	5	-0.3674333903303548
2212	6	-0.20412138333409047
2212	7	-0.2888889658667182
2212	8	-0.45977234016744717
2212	9	-0.5253854713001758
2212	10	-0.5550248552970771
2212	11	-0.5064862396359757
2212	12	-0.6504325995902605
2212	13	-0.5760273641463911
2212	14	-0.5463037487864142
2212	15	-0.5624851398708799
2212	16	-0.7363313748342648
2212	17	-0.5473928394208798
2212	18	-0.7909011116095144
2212	19	-0.7709177212951772
2212	20	-0.7114395502143509
2212	21	-0.607770998329606
2212	22	-0.5900922188252622
2212	23	-0.7265226843862322
2212	24	-0.6682773928251322
2212	25	-0.6600607897559172
2213	1	-0.3763545162197097
2213	2	-0.4305130639528443
2213	3	-0.41116163456595345
2213	4	-0.2852447613958944
2213	5	-0.2901029111441531
2213	6	-0.31591297742417623
2213	7	-0.4963683945268613
2213	8	-0.4712059429645947
2213	9	-0.5605702252203741
2213	10	-0.6500770466312744
2213	11	-0.5666118157291962
2213	12	-0.5860768456764447
2213	13	-0.6515341241364396
2213	14	-0.7975986957549992
2213	15	-0.6964907503329627
2213	16	-0.6975810637917732
2213	17	-0.7299099121973782
2213	18	-0.7900737917642644
2213	19	-0.6725974570632545
2213	20	-0.7484823177587359
2213	21	-0.8018928801951404
2213	22	-0.9482533888129012
2213	23	-0.8283537409948991
2213	24	-0.7921517912104719
2213	25	-0.8095725328061079
2214	1	-0.5378951977685169
2214	2	-0.49497965076616524
2214	3	-0.40111365459237547
2214	4	-0.640984085368153
2214	5	-0.5766916275635268
2214	6	-0.7201747095655335
2214	7	-0.5918036832563018
2214	8	-0.6197349910559353
2214	9	-0.7074348487080258
2214	10	-0.7131048093486854
2214	11	-0.8506127923728783
2214	12	-0.9232690892016038
2214	13	-0.7734177393701955
2214	14	-0.9176003897194178
2214	15	-0.897344738442797
2214	16	-0.7697666909219762
2214	17	-0.8088136244367377
2214	18	-0.7505401129806941
2214	19	-0.7900757191603276
2214	20	-0.8051540541193205
2214	21	-0.8604010517435441
2214	22	-0.7836452723183456
2214	23	-0.7872144558165104
2214	24	-0.7937357002215819
2214	25	-0.734005773616218
2215	1	-0.7527653816107609
2215	2	-0.696153058817373
2215	3	-0.6763020320274613
2215	4	-0.7918464735115407
2215	5	-0.8700474077842699
2215	6	-0.7616489239223085
2215	7	-0.7280152292776592
2215	8	-0.8682201639245903
2215	9	-0.9042218120879326
2215	10	-0.951086548539898
2215	11	-0.8509706971579973
2215	12	-0.9762014531217886
2215	13	-1.029050355239761
2215	14	-1.0719985653533612
2215	15	-0.9931308415521158
2215	16	-0.9690900365249675
2215	17	-0.9166377997812489
2215	18	-1.1414616470008738
2215	19	-1.1272040990221512
2215	20	-1.152770408250447
2215	21	-1.2362082212194494
2215	22	-1.2210233670904884
2215	23	-1.2664775435046138
2215	24	-1.3937000006807594
2215	25	-1.5030520892235444
2216	1	-1.2846617868039516
2216	2	-1.2998684384567056
2216	3	-1.305682182511159
2216	4	-1.2420678701753687
2216	5	-1.505371211869317
2216	6	-1.4392747152579375
2216	7	-1.3315040151483828
2216	8	-1.3059259539884636
2216	9	-1.548193720819441
2216	10	-1.517961670060437
2216	11	-1.4712438080715486
2216	12	-1.4297567007913443
2216	13	-1.5004210335414214
2216	14	-1.645071203758377
2216	15	-1.5954449594566924
2216	16	-1.8421689114557225
2216	17	-1.818899579783519
2216	18	-1.9438554946070923
2216	19	-1.8707888540184001
2216	20	-1.8683863018528726
2216	21	-1.927553635477615
2216	22	-1.909224227241907
2216	23	-1.9199086720834302
2216	24	-2.1007381034073447
2216	25	-2.2088141498694753
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	41831.0
3	4.0
4	406.0
5	2297.0
6	2979.0
7	3203.0
8	2624.0
9	2630.0
10	2692.0
11	3743.0
12	4015.0
13	3482.0
14	1944.0
15	2012.0
16	2146.0
17	2341.0
18	2689.0
19	3170.0
20	3880.0
21	4931.0
22	6150.0
23	7322.0
24	8670.0
25	10016.0
26	11183.0
27	12861.0
28	14835.0
29	17999.0
30	22759.0
31	29566.0
32	38061.0
33	50516.0
34	69595.0
35	97440.0
36	146435.0
37	269680.0
38	763221.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.99830168139543	17.105729034766924	11.790411881268927	26.105557402568724
2	17.130202722378073	20.334491324651793	36.83754528209411	25.69776067087602
3	18.572162190179835	24.868306017805278	27.895035506890753	28.664496285124137
4	13.028537250890121	15.533405130436398	35.78144674159216	35.65661087708133
5	16.12637440009971	35.34146003850627	31.82238457256299	16.709780988831024
6	33.89368234660836	35.37080262743025	16.27415860730372	14.461356418657681
7	30.24906317309612	30.279876100857788	21.098402943174126	18.37265778287197
8	28.103000411852975	30.98507197835104	20.09428855246569	20.817639057330293
9	28.638285476587495	14.442076215159357	18.27369271545725	38.6459455927959
10	17.24171202923661	25.16958898904646	29.193218478083033	28.3954805036339
11	37.46921867116287	21.523872446393018	21.378412771516185	19.62849611092792
12	25.956528575223782	23.427412383349992	27.02370560733307	23.59235343409316
13	29.612014762494397	19.075428926587428	24.90924004952952	26.40331626138866
14	25.162348220070964	19.934386268327405	23.783833585848246	31.11943192575339
15	26.186240299106128	25.82861864571125	21.80950052215893	26.175640533023692
16	26.71028816198439	25.096850798590083	22.884606352834354	25.308254686591177
17	25.427213141406813	25.053757289825707	23.849329050011892	25.66970051875559
18	26.841153886913883	23.051184041927993	25.41469165775504	24.692970413403085
19	27.445358251849495	24.362402526540965	23.677769667536307	24.51446955407323
20	26.79946453701903	23.55537302656419	23.49991177988449	26.145250656532294
21	28.164398972114974	24.015099093825523	22.824431480454347	24.996070453605153
22	26.93812569471457	23.412678819379632	23.394494308007086	26.254701177898713
23	25.16851009555078	22.749239273195016	24.103281799223524	27.978968832030677
24	25.54124830140241	24.729353271572716	23.299852070738723	26.42954635628615
25	25.683464803030276	23.79917619949917	23.810555990074896	26.706803007395656
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	131.0
1	131.0
2	334.5
3	538.0
4	538.0
5	538.0
6	1081.5
7	1625.0
8	1625.0
9	1625.0
10	2211.0
11	2797.0
12	2797.0
13	2797.0
14	4086.5
15	5376.0
16	5376.0
17	5376.0
18	8166.0
19	10956.0
20	10956.0
21	10956.0
22	16948.5
23	22941.0
24	22941.0
25	22941.0
26	33918.5
27	44896.0
28	44896.0
29	44896.0
30	53108.0
31	61320.0
32	61320.0
33	61320.0
34	81551.0
35	101782.0
36	101782.0
37	101782.0
38	113437.0
39	125092.0
40	125092.0
41	125092.0
42	142464.0
43	159836.0
44	159836.0
45	159836.0
46	181225.5
47	202615.0
48	202615.0
49	202615.0
50	215857.5
51	229100.0
52	229100.0
53	229100.0
54	206621.5
55	184143.0
56	184143.0
57	184143.0
58	172568.0
59	160993.0
60	160993.0
61	160993.0
62	145747.0
63	130501.0
64	130501.0
65	130501.0
66	110720.0
67	90939.0
68	90939.0
69	90939.0
70	71293.0
71	51647.0
72	51647.0
73	51647.0
74	43369.0
75	35091.0
76	35091.0
77	35091.0
78	30333.5
79	25576.0
80	25576.0
81	25576.0
82	19162.0
83	12748.0
84	12748.0
85	12748.0
86	9403.0
87	6058.0
88	6058.0
89	6058.0
90	4208.0
91	2358.0
92	2358.0
93	2358.0
94	1287.5
95	217.0
96	217.0
97	217.0
98	134.5
99	52.0
100	52.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1781555212636462
2	0.020726903280841154
3	0.029772459336930786
4	0.0441495020750865
5	0.030491311473838576
6	0.06481650101118534
7	0.07170550065655162
8	0.07529976134109054
9	0.05666951012623044
10	0.04402969338560187
11	0.6259404982124543
12	0.5595664842379688
13	0.5333882855855769
14	0.5211677992581446
15	0.5339873290330001
16	0.5107444432729817
17	0.5165551647129862
18	0.5137396605100975
19	0.531710963932792
20	0.5217069383608254
21	0.5290152684193878
22	0.5136198518206129
23	0.5238634947715487
24	0.5031365914907077
25	0.5085279825175161
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1669328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.021360364258925
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.38850640979643	40.21737945179593
2	6.881460720137484	6.333883672678797
3	1.940271139450342	2.678817519390464
4	0.9190372623292473	1.69181380151345
5	0.5564421622733421	1.2804112635924458
6	0.3906240019019799	1.0786228775075988
7	0.2515893619723741	0.8104939283801225
8	0.1953003456999172	0.7190390070976187
9	0.15720317361839706	0.6511233513147671
>10	1.023096541163564	9.167424707680384
>50	0.12475556305809542	4.080393102112366
>100	0.1334090925482389	13.378731028675912
>500	0.024399267278872008	7.711399529258189
>1k	0.013904958771830284	10.200466759001918
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	4453	0.2667540471375308	No Hit
GTATCTGATCGTCTTCGAACCTCCG	3121	0.18696145994076657	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2970	0.17791590388467696	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2921	0.17498059099230348	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2880	0.17252451285786854	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2816	0.16869063479436036	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2733	0.16371857418074817	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	2650	0.15874651356713598	No Hit
CTTCCGTACTCTGCGTTGATACCAC	2592	0.1552720615720817	No Hit
CTTTAATATACGCTATTGGAGCTGG	2526	0.15131837481908889	No Hit
GAACTACGACGGTATCTGATCGTCT	2457	0.14718497503186911	No Hit
GTACAAAGGGCAGGGACTTAATCAA	2375	0.14227281876299924	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2373	0.1421530100735146	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2350	0.14077521014444136	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	2275	0.13628238428876768	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	2238	0.13406592353330204	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	2156	0.12915376726443215	No Hit
GAGTACGGAAGCAGTGGTATCAACG	2114	0.1266377847852549	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2083	0.12478075009824312	No Hit
GCTTTGAACACTCTAATTTTTTCAA	2069	0.12394208927185071	No Hit
CTCTAGATAACCTCGGGCCGATCGC	2050	0.12280390672174671	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	2023	0.12118648941370419	No Hit
GAATAATGGAATAGGACCGCGGTTC	1940	0.11621442880009202	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1930	0.11561538535266885	No Hit
GAATAATTGCAATCCCCGATCCCCA	1929	0.11555548100792654	No Hit
CCATTATTCCTAGCTGCGGTATCCA	1926	0.11537576797369958	No Hit
TCCGTACTCTGCGTTGATACCACTG	1907	0.1142375854235956	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1886	0.11297959418400698	No Hit
TCGTAGTTCCGACCATAAACGATGC	1838	0.11010418563637585	No Hit
CTCCAATAGCGTATATTAAAGTTGC	1829	0.109565046533695	No Hit
CTTTAAATCCTTTAACGAGGATCCA	1790	0.10722877708874469	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	1786	0.10698915970977543	No Hit
AGATAACCTCGGGCCGATCGCACGC	1697	0.10165767302770935	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	1695	0.10153786433822472	No Hit
GTTCTATTTTGTTGGTTTTCGGAAC	1671	0.10010016006440917	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	5.990434474231547E-5
9	0.0	0.0	0.0	0.0	5.990434474231547E-5
10	0.0	0.0	0.0	0.0	5.990434474231547E-5
11	0.0	0.0	0.0	0.0	5.990434474231547E-5
12	0.0	0.0	0.0	0.0	5.990434474231547E-5
13	0.0	0.0	0.0	5.990434474231547E-5	5.990434474231547E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCTTAC	75	3.261448E-9	16.47494	18
TAGATCG	65	1.8850915E-7	16.09809	5
TCGACGC	85	1.702756E-8	14.538463	14
ACGCTTA	85	1.7054845E-8	14.536712	17
TAATACT	100	6.9849193E-10	14.196878	4
CTCGACG	90	3.6083293E-8	13.731184	13
AACCGCG	275	0.0	13.489653	7
CGTCGTA	345	0.0	12.958287	10
ATTACAC	75	1.5366082E-5	12.619448	3
ATCGCCA	480	0.0	12.475014	16
GACGCTT	100	1.4332363E-7	12.356206	16
TTACCCT	100	1.5145451E-7	12.303962	4
TCGCCAG	500	0.0	12.166109	17
CGACGCT	110	3.7811333E-8	12.098441	15
CCGACCA	365	0.0	11.987643	9
CCGTCGT	375	0.0	11.921624	9
CGTCAAT	295	0.0	11.921241	19
CGCCAGT	505	0.0	11.85744	18
CGCGCTA	185	0.0	11.816745	16
ATACCGT	405	0.0	11.743836	6
>>END_MODULE