FastQCFastQC Report
Thu 2 Feb 2017
SRR4050276_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050276_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1669328
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCA39050.2339264662187419No Hit
GAATAGGACCGCGGTTCTATTTTGT30680.18378652966942385No Hit
GATTAAGAGGGACGGCCGGGGGCAT30660.18366672097993925No Hit
CTATTGGAGCTGGAATTACCGCGGC30040.17995265160591567No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG27190.16287991335435578No Hit
CTCTTAATCATGGCCTCAGTTCCGA26990.16168182645950946No Hit
GTATCTGATCGTCTTCGAACCTCCG26820.1606634525988901No Hit
CTTTAATATACGCTATTGGAGCTGG26750.1602441221856939No Hit
GAATAACGCCGCCGCATCGCCAGTC24910.14922172275310783No Hit
GTCCTATTCCATTATTCCTAGCTGC24840.14880239233991163No Hit
ATATTAAAGTTGCTGCAGTTAAAAA24700.14796373151351921No Hit
CTTCCGTACTCTGCGTTGATACCAC24610.14742459241083836No Hit
GAACTACGACGGTATCTGATCGTCT24560.1471250706871268No Hit
ATACAGGACTCTTTCGAGGCCCTGT24270.14538784468959964No Hit
ATCAGATACCGTCGTAGTTCCGACC22450.13448525394649824No Hit
GAGTACGGAAGCAGTGGTATCAACG21240.12723682823267804No Hit
GTCTTGCGCCGGTCCAAGAATTTCA21200.1269972108537088No Hit
TCGTAGTTCCGACCATAAACGATGC20240.12124639375844651No Hit
GCGCAAGACGGACCAGAGCGAAAGC20140.12064735031102336No Hit
CTCTAGATAACCTCGGGCCGATCGC20050.12010821120834253No Hit
CCATTATTCCTAGCTGCGGTATCCA20040.12004830686360021No Hit
GAATAATTGCAATCCCCGATCCCCA20010.11986859382937326No Hit
TCCGTACTCTGCGTTGATACCACTG19620.11753232438442296No Hit
GAATAATGGAATAGGACCGCGGTTC19240.11525595928421495No Hit
GCTTTGAACACTCTAATTTTTTCAA19200.1150163419052457No Hit
GTACAAAGGGCAGGGACTTAATCAA18410.11028389867060277No Hit
AAATAGAACCGCGGTCCTATTCCAT18340.10986456825740658No Hit
CTTTAAATCCTTTAACGAGGATCCA18300.10962495087843731No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC18110.10848676832833332No Hit
CTCCAATAGCGTATATTAAAGTTGC18010.10788772488091015No Hit
CTCCCGACCCGGGGAGGTAGTGACG17850.10692925536503312No Hit
GGGTAGGCACACGCTGAGCCAGTCA17570.10525193371224828No Hit
GTCCTGTATTGTTATTTTTCGTCAC17530.10501231633327902No Hit
AGATAACCTCGGGCCGATCGCACGC17220.10315528164626725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT902.1809683E-914.8284271
CGAACGA1600.014.23973316
GTCCTAC852.5892587E-713.4577321
CGCGCCA500.001507454813.29081510
CGTTTCG500.001507795113.29041815
GTATTAG3200.012.8093781
ATCGCCA3900.012.41412616
TATTAGC2550.012.31292252
GGTTATA550.002990961512.1323491
CGTCTTA550.003085217412.08219815
CCGGTAG802.8869936E-511.8667999
CATCGCC4000.011.86644515
CGTCGTA3200.011.57012910
TAGATCG752.0722878E-411.4010085
CGCCAGT4350.011.3481418
ATTAGCT2700.011.2764813
TCGCCAG4300.011.25932417
CGGTTCT5650.011.25770712
TAGAGTG3550.011.240435
GAGCGTT855.3444244E-511.1720997
Produced by FastQC (version 0.11.5)