##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050276_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1669328
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.075460904028446	31.0	31.0	34.0	28.0	34.0
2	30.888577319735845	31.0	31.0	34.0	27.0	34.0
3	31.222874114613784	31.0	31.0	34.0	27.0	34.0
4	34.91654006881811	37.0	35.0	37.0	32.0	37.0
5	34.81543591193582	37.0	35.0	37.0	32.0	37.0
6	34.83896034811613	37.0	35.0	37.0	32.0	37.0
7	34.76564701484669	37.0	35.0	37.0	32.0	37.0
8	34.743677695455894	37.0	35.0	37.0	32.0	37.0
9	36.3738618174499	39.0	37.0	39.0	32.0	39.0
10	36.2499532746111	39.0	37.0	39.0	32.0	39.0
11	36.26589262266014	39.0	37.0	39.0	32.0	39.0
12	36.145595712765854	39.0	37.0	39.0	32.0	39.0
13	36.160392684960655	39.0	37.0	39.0	32.0	39.0
14	37.363503757200505	40.0	38.0	41.0	32.0	41.0
15	37.35097596158454	40.0	38.0	41.0	32.0	41.0
16	37.32757253218061	40.0	38.0	41.0	32.0	41.0
17	37.24347162450998	40.0	37.0	41.0	32.0	41.0
18	37.21798052869178	40.0	37.0	41.0	32.0	41.0
19	37.25472525471327	40.0	37.0	41.0	32.0	41.0
20	37.16713072565727	40.0	37.0	41.0	31.0	41.0
21	37.097116324652795	40.0	37.0	41.0	31.0	41.0
22	37.01724406467752	40.0	37.0	41.0	31.0	41.0
23	36.94383009210892	40.0	37.0	41.0	31.0	41.0
24	36.87776398646641	40.0	37.0	41.0	31.0	41.0
25	36.687318489835434	40.0	37.0	41.0	31.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4934711474460691
1101	2	0.8272946951165672
1101	3	0.653510482795923
1101	4	0.6437388654957346
1101	5	0.6121400388627265
1101	6	0.6243396875318084
1101	7	0.7240526580066842
1101	8	0.6908920035647199
1101	9	0.7016088414556307
1101	10	0.7663555567909413
1101	11	0.7240886494938721
1101	12	0.7246230104078322
1101	13	0.8033002375206166
1101	14	0.9040009743730693
1101	15	0.8774307558382262
1101	16	0.8098378743204293
1101	17	0.8566841138513226
1101	18	0.8097336773187749
1101	19	0.8899732035905714
1101	20	0.9050464419637123
1101	21	0.5051008136757602
1101	22	0.8021493514404412
1101	23	0.7569164380193456
1101	24	0.8917595200984465
1101	25	0.7862551324528297
1102	1	0.4556554231373582
1102	2	0.6847823137711266
1102	3	0.5786473654482833
1102	4	0.37825272572410285
1102	5	0.5277787099316456
1102	6	0.49923994891576484
1102	7	0.5975249375499558
1102	8	0.47484406024376824
1102	9	0.6181449881057901
1102	10	0.638947729029212
1102	11	0.5564051648329809
1102	12	0.5787656022361389
1102	13	0.658132573467789
1102	14	0.5920442059042301
1102	15	0.6891871872593285
1102	16	0.7277572923971931
1102	17	0.684170872690224
1102	18	0.6457105998665824
1102	19	0.7589949053377154
1102	20	0.7126030178338425
1102	21	0.8141737604359705
1102	22	0.7075340329647233
1102	23	0.7509310892781116
1102	24	0.5268523801892471
1102	25	0.5810339878662276
1103	1	0.4424325595971368
1103	2	0.7164203108801139
1103	3	0.5443479212195683
1103	4	0.536277863853698
1103	5	0.5377188565967188
1103	6	0.4628454346582416
1103	7	0.5803252360034037
1103	8	0.562234368096739
1103	9	0.5901576920303455
1103	10	0.40342041721787325
1103	11	0.4394150041736751
1103	12	0.5879440284702255
1103	13	0.6569147695403146
1103	14	0.7456450630430211
1103	15	0.7302345325213153
1103	16	0.7190845902481797
1103	17	0.7151837033422908
1103	18	0.6766322815880699
1103	19	0.6687806748549434
1103	20	0.812441762160752
1103	21	0.7579353785361818
1103	22	0.8238509113747625
1103	23	0.8711077352278807
1103	24	0.8612012278160179
1103	25	0.7424907646367345
1104	1	0.2588701702473699
1104	2	0.6106253140090736
1104	3	0.48549202459831164
1104	4	0.3708086266249424
1104	5	0.46216446883015294
1104	6	0.45818876462193003
1104	7	0.4169733963345905
1104	8	0.32056355622487587
1104	9	0.3712193191972162
1104	10	0.3804733635227535
1104	11	0.5544130251724795
1104	12	0.5508852253915393
1104	13	0.5152831365434167
1104	14	0.5954789874023874
1104	15	0.5944964453061985
1104	16	0.5828631186201036
1104	17	0.5914954602139701
1104	18	0.5770477381222463
1104	19	0.6148849842637532
1104	20	0.5094533366434035
1104	21	0.6018556996692439
1104	22	0.6454406109409874
1104	23	0.6249213240128313
1104	24	0.5561569142352525
1104	25	0.5470884657861603
1105	1	0.22097114744606827
1105	2	0.5307946951165654
1105	3	0.4044438161292554
1105	4	0.2746555321623987
1105	5	0.12924003886272573
1105	6	0.10340635419847644
1105	7	0.23071932467335188
1105	8	0.2726586702313867
1105	9	0.24372550812230287
1105	10	0.34712222345761035
1105	11	0.2943719828272009
1105	12	0.4899230104078285
1105	13	0.4277502375206126
1105	14	0.5544509743730686
1105	15	0.4531974225048927
1105	16	0.4982878743204253
1105	17	0.4426674471846539
1105	18	0.5116503439854441
1105	19	0.4003898702572428
1105	20	0.4002631086303765
1105	21	0.40295081367575847
1105	22	0.4726660181071054
1105	23	0.5286164380193412
1105	24	0.6005595200984502
1105	25	0.5478884657861585
1106	1	0.2869149225464689
1106	2	0.6547143738314247
1106	3	0.5086108844023478
1106	4	0.38713745987324444
1106	5	0.40582477781855175
1106	6	0.45626739837518215
1106	7	0.3528478387295806
1106	8	0.39208678268119
1106	9	0.37641305832310223
1106	10	0.3752812596021826
1106	11	0.46779848885130093
1106	12	0.41382983771706705
1106	13	0.43904822948848476
1106	14	0.4570582032887316
1106	15	0.5465371815410407
1106	16	0.6073529345613906
1106	17	0.6571911419637715
1106	18	0.6255619905717893
1106	19	0.46304047266687576
1106	20	0.5211257592327883
1106	21	0.6503869582540673
1106	22	0.658238708870158
1106	23	0.6615047914329963
1106	24	0.6531651425884064
1106	25	0.6380523212078444
1107	1	0.31880401429932803
1107	2	0.6477655067918953
1107	3	0.3630053515151026
1107	4	0.3525411779041008
1107	5	0.44792472498827607
1107	6	0.47927104832082534
1107	7	0.4618228832499227
1107	8	0.42698190093910426
1107	9	0.4241976392698419
1107	10	0.4404402562444929
1107	11	0.41169177491036635
1107	12	0.4868631143662441
1107	13	0.5262655513950634
1107	14	0.4593093510224122
1107	15	0.5952039798819442
1107	16	0.5023433721213095
1107	17	0.49476005014346924
1107	18	0.36020532199423627
1107	19	0.39851062195656084
1107	20	0.6043435564512478
1107	21	0.577476363455844
1107	22	0.5092483451762817
1107	23	0.17456237964269405
1107	24	0.28408658927077823
1107	25	0.3562647952543756
1108	1	0.0300286366912772
1108	2	0.40330466774073415
1108	3	0.1747436988042672
1108	4	0.22152451925665417
1108	5	0.21008978465857808
1108	6	0.26106892752659405
1108	7	0.0795382531051132
1108	8	0.036388353453915556
1108	9	0.08594905133700337
1108	10	0.028834699014900878
1108	11	-0.004119374231848383
1108	12	-0.013092085407578224
1108	13	0.13054554452100575
1108	14	0.040135604799353075
1108	15	0.15011912762808777
1108	16	0.06424000572441457
1108	17	0.11617749802548616
1108	18	0.029621403821188608
1108	19	-0.02937156892930659
1108	20	0.22744199013213517
1108	21	0.2804786979151004
1108	22	0.1312764209229087
1108	23	0.15635034494152222
1108	24	0.06656202303157244
1108	25	0.01707282245413211
1109	1	-0.11899046483799935
1109	2	0.2951401845599442
1109	3	0.09659429597570579
1109	4	0.13114689492631726
1109	5	0.006856929457732974
1109	6	5.073138913758157E-4
1109	7	-0.009990848071367964
1109	8	0.027765004204518107
1109	9	-0.04576239974718277
1109	10	-0.04221290130247013
1109	11	0.022880236186125558
1109	12	0.017161398123761273
1109	13	0.00917595729028875
1109	14	0.11043331602374451
1109	15	-0.049822922984553486
1109	16	-0.10324993757976841
1109	17	-0.1013717082855976
1109	18	-0.19937460802991325
1109	19	-0.14989803761223897
1109	20	-0.015266257972307073
1109	21	-0.003466844673567948
1109	22	0.016121680295206886
1109	23	0.11887900999631285
1109	24	0.13592458727695345
1109	25	-0.01922631348447368
1110	1	0.0330248902483703
1110	2	0.3028177277461239
1110	3	0.03670945912349666
1110	4	0.19717376632746664
1110	5	0.19764387763432012
1110	6	0.22622708356507815
1110	7	0.21726443023957387
1110	8	0.16941567598954776
1110	9	0.11277886704744589
1110	10	0.09559899888947143
1110	11	-0.01589135690408483
1110	12	0.2317487301775003
1110	13	0.1677172240849103
1110	14	0.07166172293353412
1110	15	0.0062231422745639975
1110	16	0.05605908353347644
1110	17	0.14430967367217562
1110	18	0.04438738813131238
1110	19	0.05969889137048057
1110	20	0.0799352775363289
1110	21	0.042982291602818634
1110	22	0.14203338854856895
1110	23	0.16340876047615893
1110	24	0.2303583684669661
1110	25	0.18806735254239726
1111	1	0.003593724413001098
1111	2	0.23899081826365887
1111	3	0.09409581156825197
1111	4	0.235792742348643
1111	5	0.1733230491249813
1111	6	0.1398661033432873
1111	7	0.19753422395119458
1111	8	0.05831978767722745
1111	9	0.16020775821731803
1111	10	0.19276281638805415
1111	11	0.08099334352655774
1111	12	0.288662349062335
1111	13	0.32006144998735664
1111	14	0.2513931256463522
1111	15	0.13345223056266775
1111	16	0.11619239731548703
1111	17	0.19681096675896015
1111	18	0.0961870220546146
1111	19	0.1637118010820231
1111	20	0.2942506418876931
1111	21	0.31058304476659515
1111	22	0.20603903217020303
1111	23	0.22033624037586463
1111	24	0.3127059283082545
1111	25	0.2651614418408954
1112	1	-0.2823746329870893
1112	2	0.08525025897018779
1112	3	-0.10432870067432987
1112	4	-0.10586127142236279
1112	5	-0.0939073845876095
1112	6	-0.09162994154461757
1112	7	-0.28152115329528016
1112	8	-0.1954865127409775
1112	9	-0.17889495490981489
1112	10	-0.15768166003753947
1112	11	-0.3108593838643614
1112	12	-0.2681794541179343
1112	13	-0.09732429571314327
1112	14	-0.13634723324455678
1112	15	-0.16472819362655144
1112	16	-0.17519293225164034
1112	17	-0.1867273250334165
1112	18	-0.18438849096601473
1112	19	-0.22754142175172376
1112	20	-0.15308804894990402
1112	21	-0.1385222259060228
1112	22	-0.04165541579879317
1112	23	-0.05393681067371148
1112	24	-0.0625421976013314
1112	25	-0.0778181809651457
1113	1	-0.31422825059757287
1113	2	-0.33152584664415485
1113	3	-0.15302074369015628
1113	4	0.026775923434030346
1113	5	-0.03900555933140737
1113	6	-0.12385444339370366
1113	7	-0.11675245862235784
1113	8	-0.20850792119072992
1113	9	-0.10653543995896086
1113	10	-0.17606543643704953
1113	11	-0.1895052180757304
1113	12	-0.22119151178931418
1113	13	-0.15685683546659845
1113	14	-0.12349244172926177
1113	15	-0.14461025243867454
1113	16	-0.20616569979544863
1113	17	-0.1382923722284417
1113	18	-0.09283122110109332
1113	19	-0.2111977896374171
1113	20	-0.2780583360648805
1113	21	-0.24145580784418286
1113	22	-0.18429440326234214
1113	23	-0.35900342654047535
1113	24	-0.29816523535678385
1113	25	-0.20592131600466246
1114	1	-0.6101483399267167
1114	2	-0.7842328209030356
1114	3	-0.48419274625295117
1114	4	-0.35965430575693347
1114	5	-0.40726535126166397
1114	6	-0.5894874264272332
1114	7	-0.5124129783797926
1114	8	-0.4935504515175779
1114	9	-0.400751536732578
1114	10	-0.5592384248650433
1114	11	-0.5134793552806016
1114	12	-0.5948408041417395
1114	13	-0.6317369844921998
1114	14	-0.8037200018802295
1114	15	-0.8132046883130499
1114	16	-0.8421386616765574
1114	17	-0.7923906756951027
1114	18	-0.7182373303455023
1114	19	-0.7507974648351095
1114	20	-0.6502474077661518
1114	21	-0.6834701437309505
1114	22	-0.7933207138943956
1114	23	-0.8230034639783952
1114	24	-0.7953381052313659
1114	25	-0.8284298154049452
1115	1	-0.6543385870672047
1115	2	-1.0325061898391859
1115	3	-0.6771089862306248
1115	4	-0.46579284541871857
1115	5	-0.4930148283939104
1115	6	-0.5179169496363372
1115	7	-0.4216110588074713
1115	8	-0.4614752530724431
1115	9	-0.5448107750635387
1115	10	-0.5786370685777911
1115	11	-0.6903766897391677
1115	12	-0.43094483619984203
1115	13	-0.5836798509749599
1115	14	-0.7037460757744185
1115	15	-0.6719571497664916
1115	16	-0.6339858719922589
1115	17	-0.6513136737592973
1115	18	-0.5915826943626428
1115	19	-0.7066897757604593
1115	20	-0.7273540005141683
1115	21	-0.7436049385366346
1115	22	-0.6319103830728352
1115	23	-0.7714242699452569
1115	24	-0.6604086214944758
1115	25	-0.5904484073701823
1116	1	-0.777675049343884
1116	2	-1.1342073983935528
1116	3	-0.726526735161741
1116	4	-0.5359264636505827
1116	5	-0.5497109730005008
1116	6	-0.6409196751106663
1116	7	-0.6013323152429066
1116	8	-0.5329106249535371
1116	9	-0.6815875414241077
1116	10	-0.6681763110853112
1116	11	-0.6527498594617
1116	12	-0.7965127188315293
1116	13	-0.8504881434982252
1116	14	-1.0782800793603542
1116	15	-1.004243470726088
1116	16	-0.9573071012552887
1116	17	-1.083572050372915
1116	18	-0.9302980160982983
1116	19	-0.8788145958976799
1116	20	-0.7978167238888147
1116	21	-0.8455647480827935
1116	22	-0.8597769686339944
1116	23	-0.8890552995940553
1116	24	-0.9177799076754525
1116	25	-0.9869310736416139
1201	1	0.5593148844414912
1201	2	0.7289769672763668
1201	3	0.5654289180851464
1201	4	0.41497013890397483
1201	5	0.5304265557166588
1201	6	0.5005569159962278
1201	7	0.4658296034914997
1201	8	0.44021888662756226
1201	9	0.6095192659250444
1201	10	0.5615729933286033
1201	11	0.632936443917508
1201	12	0.5749507257636353
1201	13	0.5527940993599785
1201	14	0.7234365757047385
1201	15	0.7901242639530821
1201	16	0.6419201672875516
1201	17	0.6114110177214798
1201	18	0.6390361117757024
1201	19	0.7170357295997292
1201	20	0.657731106965791
1201	21	0.7157369976125025
1201	22	0.7137135420355349
1201	23	0.6578101125927915
1201	24	0.6265202358704016
1201	25	0.6399931682414248
1202	1	0.461858244220263
1202	2	0.6977785660843061
1202	3	0.5269244612905482
1202	4	0.15213940313014263
1202	5	0.2539948775724028
1202	6	0.33253861226299364
1202	7	0.24854190531851827
1202	8	0.28546189603783745
1202	9	0.47267066941262215
1202	10	0.4661673847479335
1202	11	0.3902042408917197
1202	12	0.4576875265368585
1202	13	0.3718083020367402
1202	14	0.47581549050210015
1202	15	0.47732322895650725
1202	16	0.5600394872236549
1202	17	0.5653803504104573
1202	18	0.5190213117273785
1202	19	0.4915189025153026
1202	20	0.5292534312110249
1202	21	0.3844959749660788
1202	22	0.2549853729458178
1202	23	0.120464825116116
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2210	23	0.2127990627882923
2210	24	0.2204286513368956
2210	25	0.21353948242202136
2211	1	-0.16542351130764033
2211	2	-0.42557622476473966
2211	3	-0.3077110800131777
2211	4	-0.23304773193255812
2211	5	-0.1582382994162046
2211	6	-0.1108482452080537
2211	7	-0.19508186227026414
2211	8	-0.11063865914546511
2211	9	-0.29940624261953985
2211	10	-0.14429973499936466
2211	11	-0.14862030203926935
2211	12	-0.33943485309365684
2211	13	-0.1446485755357685
2211	14	-0.1900012511462137
2211	15	-0.20887735494317639
2211	16	-0.19719105743031662
2211	17	-0.18953373975896426
2211	18	-0.22564342456055186
2211	19	-0.13455078256175312
2211	20	-0.024284665850338172
2211	21	-0.019257494929583174
2211	22	-0.08649184539437726
2211	23	0.09579625997780283
2211	24	0.061700172917440455
2211	25	0.11756324323422973
2212	1	-0.2926129233503936
2212	2	-1.0741728565058537
2212	3	-0.5701768328382961
2212	4	-0.2666778011709354
2212	5	-0.36432751275969366
2212	6	-0.3210142947690784
2212	7	-0.3231597313738419
2212	8	-0.37961684599279977
2212	9	-0.2435570877479094
2212	10	-0.26374031341554627
2212	11	-0.23093716171557332
2212	12	-0.1824197624535273
2212	13	-0.3145058096770299
2212	14	-0.3128906185472857
2212	15	-0.26967101407327476
2212	16	-0.2800035711073008
2212	17	-1.393570310927437
2212	18	-0.899104818256447
2212	19	-0.5975452329875992
2212	20	-0.563268454791455
2212	21	-0.4228084783596415
2212	22	-0.5305092031318281
2212	23	-0.5354360693552849
2212	24	-0.46645581913458756
2212	25	-0.2674395578126578
2213	1	-0.3419992923520567
2213	2	-0.8819464728718991
2213	3	-0.5590393850243132
2213	4	-0.48092918305028576
2213	5	-0.39154957901759246
2213	6	-0.40585738048068976
2213	7	-0.3939165801572173
2213	8	-0.3792167443324175
2213	9	-0.44522214869096643
2213	10	-0.45445614712638616
2213	11	-0.4099527468050965
2213	12	-0.3250976096354279
2213	13	-0.36677636666107105
2213	14	-0.4715334524474031
2213	15	-0.4850139689875377
2213	16	-0.4072178214257889
2213	17	-1.4240569940554337
2213	18	-1.2166611196050425
2213	19	-0.9546209444507596
2213	20	-0.711840424174234
2213	21	-0.6738979246083119
2213	22	-0.5900889927075994
2213	23	-0.5878366261479258
2213	24	-0.3766110638957869
2213	25	-0.4606620749492407
2214	1	-0.6650187002696697
2214	2	-2.3062785463627655
2214	3	-1.0025542984465226
2214	4	-0.707818724545362
2214	5	-0.6275245731749806
2214	6	-0.4732068437710666
2214	7	-0.5573807478429629
2214	8	-0.4150501042428658
2214	9	-0.5887304744737847
2214	10	-0.44540598538938525
2214	11	-0.6035488293555389
2214	12	-0.5914484181636013
2214	13	-0.5153990010580642
2214	14	-0.5626242975631115
2214	15	-0.612115412883071
2214	16	-0.6061927638231523
2214	17	-0.3698166717421074
2214	18	-0.48440186776220173
2214	19	-0.5182468229987407
2214	20	-0.3566108580121181
2214	21	-0.5093018331697863
2214	22	-0.5139042502032609
2214	23	-0.4801629673468639
2214	24	-0.4558909512576079
2214	25	-0.44112763283602874
2215	1	-0.9858077848362683
2215	2	-2.3691322128017447
2215	3	-1.3227298133942114
2215	4	-0.8899908311482392
2215	5	-0.7690593520365923
2215	6	-0.8249556665826105
2215	7	-0.8896353868995561
2215	8	-0.8408997676045118
2215	9	-0.8533748860016672
2215	10	-0.9903824702005792
2215	11	-1.0656105324003136
2215	12	-1.0066605797032437
2215	13	-1.0295916471085533
2215	14	-1.0489948156663402
2215	15	-1.0723868125363083
2215	16	-0.9694459845115304
2215	17	-1.0687880884656522
2215	18	-1.1484442457673367
2215	19	-1.2283095206420782
2215	20	-1.1809216996820524
2215	21	-1.10076670692618
2215	22	-1.103009940330729
2215	23	-1.1827261631988577
2215	24	-1.005012246293525
2215	25	-1.1776517706882217
2216	1	-0.9744554737143432
2216	2	-1.9525090591496443
2216	3	-1.135886559297365
2216	4	-0.8060407135713916
2216	5	-0.8424077427072376
2216	6	-0.8299834068936747
2216	7	-0.849246545633811
2216	8	-0.8536785311338022
2216	9	-1.1395017956319649
2216	10	-1.1341023499212355
2216	11	-1.1345618055687012
2216	12	-1.0925698223566727
2216	13	-1.1636067590664183
2216	14	-1.2800732577088425
2216	15	-1.2249213488261645
2216	16	-1.3285366990584109
2216	17	-1.2785107098119326
2216	18	-1.3431346389497136
2216	19	-1.3299855563639227
2216	20	-1.360748495465188
2216	21	-1.453700380863495
2216	22	-1.4175278385481818
2216	23	-1.4646791251205897
2216	24	-1.5634281932121326
2216	25	-1.4517169949646913
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16717.0
3	0.0
4	352.0
5	2682.0
6	3233.0
7	2541.0
8	2161.0
9	1881.0
10	1803.0
11	1679.0
12	1549.0
13	1445.0
14	1558.0
15	1610.0
16	1687.0
17	1802.0
18	2103.0
19	2281.0
20	3162.0
21	3789.0
22	4980.0
23	6105.0
24	7658.0
25	9094.0
26	10477.0
27	12474.0
28	14314.0
29	17973.0
30	23289.0
31	30552.0
32	40512.0
33	54031.0
34	74783.0
35	105948.0
36	157313.0
37	278827.0
38	766963.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.92020210506103	18.014173757545937	12.467806007944986	26.597818129448054
2	16.852687263729877	20.998759862686246	36.01598399204395	26.132568881539925
3	18.674280908245713	24.562758187725837	28.151927002961667	28.61103390106678
4	13.52009868448683	16.138585466357398	35.585513155731654	34.755802693424116
5	16.01583663762724	35.60067028820475	31.99109878360431	16.392394290563704
6	33.9805813604316	35.69797835886782	16.04827867707746	14.27316160362312
7	30.070457268089395	30.428167086262057	21.116745002460043	18.3846306431885
8	28.018466866594565	31.23962287326948	19.945833720306663	20.796076539829293
9	28.276145112489143	14.383751235732904	18.372127857164255	38.9679757946137
10	17.1563097425562	25.365502375173815	29.270564919320773	28.20762296294921
11	37.60031203528731	21.34308183976197	21.418514333372077	19.638091791578642
12	25.958579583854597	23.51057379367202	26.963653183402116	23.56719343907127
13	29.77480734702338	18.92261709700436	24.85272823454208	26.449847321430177
14	25.102895204454416	19.812673813514948	23.856350506298323	31.228080475732305
15	26.286893215224726	25.933573791821896	21.728845014499996	26.050687978453386
16	26.840330194330626	25.03738049025951	22.718412287638078	25.403877027771788
17	25.571367640152204	25.141853488349803	23.835938772967324	25.450840098530662
18	26.985589410828787	22.990508755619025	25.381291154284842	24.642610679267346
19	27.51077080118467	24.2812676717817	23.66317464273049	24.544786884303143
20	26.84762970488724	23.41073773398637	23.600215176406316	26.14141738472008
21	28.31170589479246	23.90272774708208	22.823682209946604	24.961884148178857
22	26.8588161783251	23.466994484253433	23.40918308600239	26.265006251419077
23	25.18362322269913	22.667261721125133	24.328002803545168	27.82111225263057
24	25.674577144380446	24.578432341603243	23.447302919478467	26.299687594537847
25	25.765230846737918	23.63141290918469	23.944486427483298	26.658869816594095
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	67.0
1	67.0
2	149.0
3	231.0
4	231.0
5	231.0
6	576.5
7	922.0
8	922.0
9	922.0
10	1307.0
11	1692.0
12	1692.0
13	1692.0
14	2669.5
15	3647.0
16	3647.0
17	3647.0
18	6638.5
19	9630.0
20	9630.0
21	9630.0
22	16268.0
23	22906.0
24	22906.0
25	22906.0
26	34257.0
27	45608.0
28	45608.0
29	45608.0
30	53935.5
31	62263.0
32	62263.0
33	62263.0
34	82364.5
35	102466.0
36	102466.0
37	102466.0
38	114548.5
39	126631.0
40	126631.0
41	126631.0
42	143495.5
43	160360.0
44	160360.0
45	160360.0
46	181784.0
47	203208.0
48	203208.0
49	203208.0
50	215919.0
51	228630.0
52	228630.0
53	228630.0
54	206364.5
55	184099.0
56	184099.0
57	184099.0
58	172883.0
59	161667.0
60	161667.0
61	161667.0
62	146398.0
63	131129.0
64	131129.0
65	131129.0
66	110817.0
67	90505.0
68	90505.0
69	90505.0
70	71115.5
71	51726.0
72	51726.0
73	51726.0
74	43298.5
75	34871.0
76	34871.0
77	34871.0
78	30307.0
79	25743.0
80	25743.0
81	25743.0
82	19273.5
83	12804.0
84	12804.0
85	12804.0
86	9340.0
87	5876.0
88	5876.0
89	5876.0
90	4136.5
91	2397.0
92	2397.0
93	2397.0
94	1305.0
95	213.0
96	213.0
97	213.0
98	125.0
99	37.0
100	37.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17180566072096076
2	0.009464886469285845
3	0.0
4	0.15587110501950485
5	0.04744424103591385
6	0.0557709449550957
7	0.03935715449570126
8	0.02354240748372998
9	0.0166534078383637
10	0.011202112466812993
11	0.017132642596302226
12	0.017731686043725377
13	0.008087086540212588
14	0.009225269090316582
15	0.001018373860619363
16	4.792347579385238E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.00449282585567366
22	0.0064097648874277555
23	7.787564816501011E-4
24	0.0013777999290732557
25	0.11908983734772315
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1669328.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.55090270964892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.1161082658618	39.682173720615125
2	7.04901775613076	6.4217824401620005
3	1.984462826992595	2.711822194897637
4	0.9820841291793846	1.7893927448376172
5	0.5488677801323211	1.2500711426634166
6	0.3617272867192763	0.9886202666865022
7	0.2769322841344272	0.8830160872237702
8	0.19414605390120537	0.707482241017285
9	0.14695538341138775	0.6024555335188151
>10	1.0356928734974618	9.13144832833708
>50	0.12558290493560587	4.01215437824372
>100	0.1384632998547472	13.60542844590163
>500	0.02588716037738527	8.043180580224012
>1k	0.01407199487180943	10.170971895671405
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACGGAAGCAGTGGTATCAACGCA	3905	0.2339264662187419	No Hit
GAATAGGACCGCGGTTCTATTTTGT	3068	0.18378652966942385	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	3066	0.18366672097993925	No Hit
CTATTGGAGCTGGAATTACCGCGGC	3004	0.17995265160591567	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2719	0.16287991335435578	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	2699	0.16168182645950946	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2682	0.1606634525988901	No Hit
CTTTAATATACGCTATTGGAGCTGG	2675	0.1602441221856939	No Hit
GAATAACGCCGCCGCATCGCCAGTC	2491	0.14922172275310783	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2484	0.14880239233991163	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	2470	0.14796373151351921	No Hit
CTTCCGTACTCTGCGTTGATACCAC	2461	0.14742459241083836	No Hit
GAACTACGACGGTATCTGATCGTCT	2456	0.1471250706871268	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	2427	0.14538784468959964	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2245	0.13448525394649824	No Hit
GAGTACGGAAGCAGTGGTATCAACG	2124	0.12723682823267804	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2120	0.1269972108537088	No Hit
TCGTAGTTCCGACCATAAACGATGC	2024	0.12124639375844651	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2014	0.12064735031102336	No Hit
CTCTAGATAACCTCGGGCCGATCGC	2005	0.12010821120834253	No Hit
CCATTATTCCTAGCTGCGGTATCCA	2004	0.12004830686360021	No Hit
GAATAATTGCAATCCCCGATCCCCA	2001	0.11986859382937326	No Hit
TCCGTACTCTGCGTTGATACCACTG	1962	0.11753232438442296	No Hit
GAATAATGGAATAGGACCGCGGTTC	1924	0.11525595928421495	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1920	0.1150163419052457	No Hit
GTACAAAGGGCAGGGACTTAATCAA	1841	0.11028389867060277	No Hit
AAATAGAACCGCGGTCCTATTCCAT	1834	0.10986456825740658	No Hit
CTTTAAATCCTTTAACGAGGATCCA	1830	0.10962495087843731	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1811	0.10848676832833332	No Hit
CTCCAATAGCGTATATTAAAGTTGC	1801	0.10788772488091015	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	1785	0.10692925536503312	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1757	0.10525193371224828	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	1753	0.10501231633327902	No Hit
AGATAACCTCGGGCCGATCGCACGC	1722	0.10315528164626725	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	5.990434474231547E-5	5.990434474231547E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAT	90	2.1809683E-9	14.828427	1
CGAACGA	160	0.0	14.239733	16
GTCCTAC	85	2.5892587E-7	13.457732	1
CGCGCCA	50	0.0015074548	13.290815	10
CGTTTCG	50	0.0015077951	13.290418	15
GTATTAG	320	0.0	12.809378	1
ATCGCCA	390	0.0	12.414126	16
TATTAGC	255	0.0	12.3129225	2
GGTTATA	55	0.0029909615	12.132349	1
CGTCTTA	55	0.0030852174	12.082198	15
CCGGTAG	80	2.8869936E-5	11.866799	9
CATCGCC	400	0.0	11.866445	15
CGTCGTA	320	0.0	11.570129	10
TAGATCG	75	2.0722878E-4	11.401008	5
CGCCAGT	435	0.0	11.34814	18
ATTAGCT	270	0.0	11.276481	3
TCGCCAG	430	0.0	11.259324	17
CGGTTCT	565	0.0	11.257707	12
TAGAGTG	355	0.0	11.24043	5
GAGCGTT	85	5.3444244E-5	11.172099	7
>>END_MODULE