FastQCFastQC Report
Thu 2 Feb 2017
SRR4050275_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050275_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8230698
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAAGGGCAGGGACTTAATCAA185350.22519353765622308No Hit
GAATAGGACCGCGGTTCTATTTTGT134180.1630238407483788No Hit
GTATCTGATCGTCTTCGAACCTCCG133210.16184532587637163No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG123890.15052186339481777No Hit
CTATTGGAGCTGGAATTACCGCGGC123090.14954989236635824No Hit
GTCCTATTCCATTATTCCTAGCTGC122570.14891811119785953No Hit
GTATCAACGCAGAGTACTTTTTTTT119830.14558911042538555No Hit
GATTAAGAGGGACGGCCGGGGGCAT119800.1455526615118183No Hit
GAACTACGACGGTATCTGATCGTCT104230.12663567537042425No Hit
CTTTAATATACGCTATTGGAGCTGG103750.1260524927533485No Hit
ATACAGGACTCTTTCGAGGCCCTGT103230.1254207115848498No Hit
GTATCAACGCAGAGTACGGGCGGTG102760.12484967860562979No Hit
CTCTTAATCATGGCCTCAGTTCCGA102600.12465528439993791No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC99230.12056085644255202No Hit
GTCTTGCGCCGGTCCAAGAATTTCA98560.11974683070621714No Hit
ATCAGATACCGTCGTAGTTCCGACC95810.11640568029588742No Hit
TCGTAGTTCCGACCATAAACGATGC92740.11267574147417388No Hit
GGGTAGGCACACGCTGAGCCAGTCA91220.11082899652010071No Hit
GTCCTGTATTGTTATTTTTCGTCAC90560.11002712042162159No Hit
CTTATGACCCGCACTTACTGGGAAT89790.10909159830672928No Hit
ATATTAAAGTTGCTGCAGTTAAAAA89120.1082775725703944No Hit
GACTTAATCAACGCAAGCTTATGAC88430.10743924755834804No Hit
GCTTTGAACACTCTAATTTTTTCAA88320.10730560154193484No Hit
GAATAACGCCGCCGCATCGCCAGTC84890.10313827575741451No Hit
GAATAATTGCAATCCCCGATCCCCA84090.10216630472895494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49050.015.9948771
GTATCAA172050.013.7460211
ACCGTCC5000.011.9834998
AGGCCCG23300.011.67416710
AACCGCG10450.011.6488467
CGAACGA8300.011.45693916
CGGTCCA20900.011.28550910
ACGCTTA2800.011.20686117
TAGATCG2550.011.1885275
CGTCGTA17800.011.1137110
CGAGCCG24500.010.94535115
CGGTTCT25550.010.94175912
ACTCTAA18200.010.92171110
TCCATTA23200.010.6585588
CGCCAGT15800.010.65225518
CCGTCGT18400.010.6478649
CTCGACG2950.010.63773613
CCGTCCA4300.010.6166119
GTATTAG15600.010.6164391
ATACCGT19200.010.6000296
Produced by FastQC (version 0.11.5)