##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4050275_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8230698
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.489423618750195	31.0	31.0	34.0	27.0	34.0
2	30.61443452305017	31.0	31.0	34.0	27.0	34.0
3	30.719109120514446	31.0	31.0	34.0	27.0	34.0
4	34.23633852195768	37.0	35.0	37.0	30.0	37.0
5	34.23944068899139	37.0	35.0	37.0	30.0	37.0
6	34.26923718984708	37.0	35.0	37.0	30.0	37.0
7	34.19565594558323	37.0	35.0	37.0	30.0	37.0
8	34.163879296749805	37.0	35.0	37.0	30.0	37.0
9	35.73031497450155	39.0	37.0	39.0	30.0	39.0
10	35.62376228601754	39.0	37.0	39.0	30.0	39.0
11	35.50457263770339	39.0	37.0	39.0	30.0	39.0
12	35.428991198559345	39.0	37.0	39.0	30.0	39.0
13	35.429888449314994	39.0	37.0	39.0	30.0	39.0
14	36.599155502971925	40.0	37.0	41.0	30.0	41.0
15	36.54672592774027	40.0	37.0	41.0	30.0	41.0
16	36.552653128568195	40.0	37.0	41.0	30.0	41.0
17	36.522903160825486	40.0	37.0	41.0	30.0	41.0
18	36.48952433924802	40.0	37.0	41.0	30.0	41.0
19	36.5066877681577	40.0	37.0	41.0	30.0	41.0
20	36.46261750825993	40.0	37.0	41.0	30.0	41.0
21	36.413822497192825	40.0	37.0	41.0	30.0	41.0
22	36.33020358662169	40.0	37.0	41.0	30.0	41.0
23	36.25173563651588	40.0	37.0	41.0	29.0	41.0
24	36.18391380658117	40.0	37.0	41.0	29.0	41.0
25	35.95986634426388	40.0	37.0	41.0	28.0	41.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.573579143369038
1101	2	0.5867766825874554
1101	3	0.5510664241488712
1101	4	0.58017538836868
1101	5	0.5710275225434316
1101	6	0.5816520657012703
1101	7	0.5548286932831914
1101	8	0.6011273372651047
1101	9	0.6625549456330972
1101	10	0.6556946195830093
1101	11	0.5636661257000668
1101	12	0.5738372073047131
1101	13	0.5062200737089597
1101	14	0.6399631358374904
1101	15	0.7088641863665828
1101	16	0.7192581984589168
1101	17	0.7030854609780874
1101	18	0.7084509212049284
1101	19	0.7825219955433127
1101	20	0.75100434473012
1101	21	0.7548521427672412
1101	22	0.7279517189667786
1101	23	0.7572418308165467
1101	24	0.8035147948213535
1101	25	0.8518913863824693
1102	1	0.5383628459103349
1102	2	0.5257995915998279
1102	3	0.5504264107742429
1102	4	0.5208147457183543
1102	5	0.47941914174258216
1102	6	0.45719700096556437
1102	7	0.5252397621587761
1102	8	0.5026819843399224
1102	9	0.576839239364233
1102	10	0.6874380965516593
1102	11	0.6158095944309352
1102	12	0.5559768958613276
1102	13	0.544013528428799
1102	14	0.7145769439777467
1102	15	0.6291519364711817
1102	16	0.6484771607780644
1102	17	0.6963526390610539
1102	18	0.7418642993383173
1102	19	0.7335748745452193
1102	20	0.6769804593541693
1102	21	0.7358130637034961
1102	22	0.7795096265355781
1102	23	0.7046983581880824
1102	24	0.6773682993098618
1102	25	0.7535636682253468
1103	1	0.5882147160058011
1103	2	0.5572757452935377
1103	3	0.5486052723718373
1103	4	0.5574615862081203
1103	5	0.556716679707904
1103	6	0.5795188132705746
1103	7	0.5740704568735566
1103	8	0.5891435348291907
1103	9	0.6379785900037973
1103	10	0.6661877453349092
1103	11	0.5704330054592148
1103	12	0.566788931368869
1103	13	0.5590260323745255
1103	14	0.6716464039871468
1103	15	0.6668713289364803
1103	16	0.6277525892555076
1103	17	0.5213621178924583
1103	18	0.6307065854296567
1103	19	0.6106055716935117
1103	20	0.6481498821195615
1103	21	0.5781491610374943
1103	22	0.631205613594858
1103	23	0.7011922884493345
1103	24	0.7474563381085275
1103	25	0.7219012595154837
1104	1	0.46289387419011163
1104	2	0.5072158395635284
1104	3	0.5393390517654097
1104	4	0.4886320766569625
1104	5	0.5162700129725764
1104	6	0.49302827059446486
1104	7	0.4357777644571499
1104	8	0.44528023890404
1104	9	0.5465259418501134
1104	10	0.5255611897598982
1104	11	0.4664373447625252
1104	12	0.4833723479522902
1104	13	0.46431633199471634
1104	14	0.5188879367956929
1104	15	0.4054600597932563
1104	16	0.5417077635199661
1104	17	0.531074913687732
1104	18	0.5694948876200172
1104	19	0.601410871777162
1104	20	0.6191888660451426
1104	21	0.5108164364882697
1104	22	0.5580416641422516
1104	23	0.549203956048629
1104	24	0.5800201088166261
1104	25	0.658402616119929
1105	1	0.3205467299833842
1105	2	0.3865350935402283
1105	3	0.40080054545318333
1105	4	0.42788125093158413
1105	5	0.422660862107783
1105	6	0.3332060542995876
1105	7	0.4021316362005578
1105	8	0.4356904478318029
1105	9	0.42181922194568955
1105	10	0.4378380359725398
1105	11	0.37595343439736695
1105	12	0.3642783617696921
1105	13	0.43992466960227716
1105	14	0.5230218218182614
1105	15	0.5301662618516687
1105	16	0.39630576603653367
1105	17	0.38299310294116395
1105	18	0.5756276980722888
1105	19	0.46773999844819514
1105	20	0.4581489547768598
1105	21	0.508781819518255
1105	22	0.4912443090765919
1105	23	0.4694904459880007
1105	24	0.46465369771095055
1105	25	0.5289834178634649
1106	1	0.21974757111916077
1106	2	0.24997120596390587
1106	3	0.27234193997210454
1106	4	0.2901243847789132
1106	5	0.31300737041230065
1106	6	0.2665953820827127
1106	7	0.23773536683893326
1106	8	0.24161892121964712
1106	9	0.25312758234436217
1106	10	0.3066821900355663
1106	11	0.26061631418844655
1106	12	0.3310595650298893
1106	13	0.2414219566475566
1106	14	0.2656138273739046
1106	15	0.36066227071680856
1106	16	0.3249790799917349
1106	17	0.36962514916176303
1106	18	0.34523717574438706
1106	19	0.26783446151046775
1106	20	0.3359277227444366
1106	21	0.3372050078768396
1106	22	0.3615921376921776
1106	23	0.12767275667019362
1106	24	0.33417740214984093
1106	25	0.34024116581581865
1107	1	0.2567675115248882
1107	2	0.1979090819942897
1107	3	0.1835755455443575
1107	4	0.24494956639272658
1107	5	0.28198766340585735
1107	6	0.1746081808318536
1107	7	0.24820194336459167
1107	8	0.2614575504016372
1107	9	0.36885875852441075
1107	10	0.37885971755744663
1107	11	0.262026804918186
1107	12	0.2787174522861591
1107	13	0.2838931128595803
1107	14	0.309835555659447
1107	15	0.2948435439873478
1107	16	0.300949616946923
1107	17	0.3291294441991113
1107	18	0.3347989350276208
1107	19	0.38922470536882514
1107	20	0.3866422885894991
1107	21	0.3506901032413552
1107	22	0.3293772921036151
1107	23	0.23880467606172573
1107	24	0.2880342994730043
1107	25	0.3528184696461736
1108	1	0.2353099164028869
1108	2	0.1762577852661451
1108	3	0.21241096353601918
1108	4	0.25618880238816644
1108	5	0.23859219413058952
1108	6	0.1943653660888316
1108	7	0.19438229911936133
1108	8	0.17047760955949087
1108	9	0.08812090052346377
1108	10	0.07049130341205512
1108	11	0.06999696351373785
1108	12	0.09520972441230668
1108	13	0.15188553136609784
1108	14	0.09761304740662524
1108	15	0.22826331745110906
1108	16	0.18484714564252158
1108	17	0.08972410182047952
1108	18	0.03205594361863717
1108	19	0.17874223063206074
1108	20	0.16933482924790866
1108	21	0.26780777286591473
1108	22	0.26793123074500613
1108	23	0.14455490067987142
1108	24	0.20340903321216786
1108	25	0.14754032171114773
1109	1	-0.006258892491626256
1109	2	-0.014290784906648923
1109	3	0.03798189908785332
1109	4	0.04403014665633975
1109	5	0.06628934354255733
1109	6	-0.00994251929636647
1109	7	0.044217749255253125
1109	8	0.05059632000494929
1109	9	0.0379233125746552
1109	10	-0.09594190397744029
1109	11	-0.054664476187937794
1109	12	-0.027696730258753632
1109	13	-0.07055252653653099
1109	14	-0.03859240661204666
1109	15	-0.03387718088441716
1109	16	-0.07012357093653776
1109	17	-0.14245883604131393
1109	18	-0.006533303063761764
1109	19	-0.057369824893605426
1109	20	-0.06032450153501401
1109	21	-0.07093804132454551
1109	22	-0.05947493222514311
1109	23	-0.01802479969598636
1109	24	-0.03772557493299189
1109	25	-0.045153580557219186
1110	1	0.02960901818227768
1110	2	-0.027442719995200093
1110	3	0.062038681177636334
1110	4	0.01070876491494488
1110	5	0.0025307337332378665
1110	6	4.732613362747884E-4
1110	7	0.020210869903962703
1110	8	-0.0298168649040349
1110	9	-0.011272413485286847
1110	10	-0.12899912347302234
1110	11	-0.05013519231371788
1110	12	-0.07561353995226483
1110	13	-9.713609042663052E-4
1110	14	-0.02977204875570294
1110	15	-0.020893020560492914
1110	16	0.023022868991354528
1110	17	0.0396525165973145
1110	18	0.007586120146520159
1110	19	-0.02241285892541356
1110	20	-0.02260955476639026
1110	21	-0.009464923226637723
1110	22	-0.010676914389257774
1110	23	0.07733025145236638
1110	24	0.05873480722432589
1110	25	0.08454417388790603
1111	1	0.04077527598598607
1111	2	0.020949772997337135
1111	3	-0.00416189191992089
1111	4	0.12586955231807195
1111	5	0.053344549906711336
1111	6	0.060484987687992486
1111	7	0.028809246051842763
1111	8	0.021710884357332816
1111	9	0.016132329662944755
1111	10	0.056216991517764825
1111	11	-0.10166153086458252
1111	12	-0.12716025609677217
1111	13	-0.06543763281268866
1111	14	-0.05041637427910928
1111	15	-0.010185399087021096
1111	16	-0.08753252845038872
1111	17	-0.055308934781820085
1111	18	-0.022846880465557717
1111	19	-0.024636306048172685
1111	20	-0.04234520937683328
1111	21	-0.03371570623671971
1111	22	-0.0310908995801924
1111	23	0.005658596330050614
1111	24	-0.05561977705996668
1111	25	-0.04891827492332368
1112	1	-0.09003901750829613
1112	2	-0.06974247205411643
1112	3	-0.06535604084558955
1112	4	-0.08956257277140622
1112	5	-0.04048057928531534
1112	6	-0.11696544989806767
1112	7	-0.1155186469025864
1112	8	-0.08103847940170539
1112	9	-0.08911489745101164
1112	10	-0.14231503420931801
1112	11	-0.08364777030062243
1112	12	-0.17337579232401623
1112	13	-0.15465233672361478
1112	14	-0.12808114441202179
1112	15	-0.16225871565583816
1112	16	-0.2012242256405372
1112	17	-0.1194459758911961
1112	18	-0.18352213660990913
1112	19	-0.2836930096401389
1112	20	-0.15385815235393352
1112	21	-0.23183548768254525
1112	22	-0.12213385600391291
1112	23	-0.11584083782927479
1112	24	-0.11502867953058171
1112	25	-0.14373142519265514
1113	1	-0.3219845495003888
1113	2	-0.337649131261216
1113	3	-0.20784357562524391
1113	4	-0.17720401026576127
1113	5	-0.22100965101207493
1113	6	-0.22686097768738023
1113	7	-0.2833435986808084
1113	8	-0.27328443131816016
1113	9	-0.24594111391893136
1113	10	-0.3679718003910111
1113	11	-0.2863111726487375
1113	12	-0.4681806348979407
1113	13	-0.342103253620202
1113	14	-0.33510443009964064
1113	15	-0.32583740371782
1113	16	-0.26874764471070733
1113	17	-0.29812428724559226
1113	18	-0.2749392000921489
1113	19	-0.3347020689573412
1113	20	-0.2610071839773269
1113	21	-0.35307526920417587
1113	22	-0.3922493610243123
1113	23	-0.3113529981358667
1113	24	-0.3443680757600376
1113	25	-0.30967310302349915
1114	1	-0.35320241155262977
1114	2	-0.4207337150528794
1114	3	-0.41666142047638033
1114	4	-0.39703560741951094
1114	5	-0.3125312221486922
1114	6	-0.3548820817281424
1114	7	-0.43570554610897005
1114	8	-0.4216855364618368
1114	9	-0.45284106739195096
1114	10	-0.4354900907179271
1114	11	-0.5525229184477993
1114	12	-0.5258150226481249
1114	13	-0.4754085849405314
1114	14	-0.7172508551546741
1114	15	-0.7055784585948288
1114	16	-0.6130359096851592
1114	17	-0.6481228355598887
1114	18	-0.7268991994859135
1114	19	-0.5228588654394031
1114	20	-0.6884142934391804
1114	21	-0.6560312117298395
1114	22	-0.8527773418554858
1114	23	-0.674588181081134
1114	24	-0.6319703191713941
1114	25	-0.7234243237177083
1115	1	-0.39233633795043943
1115	2	-0.4854611808544007
1115	3	-0.5179625660427831
1115	4	-0.3616265836082775
1115	5	-0.37474107508221266
1115	6	-0.42069977579159
1115	7	-0.4631408025922923
1115	8	-0.5232880427203597
1115	9	-0.4721475446635566
1115	10	-0.5142108200767623
1115	11	-0.5917499179383867
1115	12	-0.6078847782596881
1115	13	-0.49067094508242803
1115	14	-0.5724355651166633
1115	15	-0.5094377004165338
1115	16	-0.5990345327398856
1115	17	-0.5233992184505851
1115	18	-0.527711193509532
1115	19	-0.5957180347903019
1115	20	-0.5812877353168915
1115	21	-0.6158425448971201
1115	22	-0.8534845918206599
1115	23	-0.6867768644484542
1115	24	-0.6140515830411104
1115	25	-0.6341764185109255
1116	1	-0.33793103414402026
1116	2	-0.431978917848884
1116	3	-0.4555214286871134
1116	4	-0.49118231617233477
1116	5	-0.4746163131691432
1116	6	-0.45280039897012614
1116	7	-0.37735090455071685
1116	8	-0.4999252426664569
1116	9	-0.5154438332645128
1116	10	-0.5174144429197938
1116	11	-0.5638637097476504
1116	12	-0.5833206275804699
1116	13	-0.6186783026429836
1116	14	-0.6223857730051918
1116	15	-0.6896765043487747
1116	16	-0.657585727559983
1116	17	-0.7848364329993203
1116	18	-0.6390380878480215
1116	19	-0.7029568472774557
1116	20	-0.6761162123558933
1116	21	-0.760547835481546
1116	22	-0.8039658350979622
1116	23	-0.7180328597668009
1116	24	-0.8224779439513767
1116	25	-0.8266933803120722
1201	1	0.5033450516968827
1201	2	0.6035265392078841
1201	3	0.594277374118704
1201	4	0.5924999460128362
1201	5	0.5869615253733116
1201	6	0.6646219941681508
1201	7	0.6326345458114773
1201	8	0.6411809349974362
1201	9	0.7246082607456188
1201	10	0.7919448500639774
1201	11	0.6361802160662364
1201	12	0.7030706844730545
1201	13	0.7669361444902449
1201	14	0.8741574149093765
1201	15	0.8870851683091985
1201	16	0.8131033797464866
1201	17	0.8029063503712734
1201	18	0.8170401012024229
1201	19	0.8650974383349137
1201	20	0.8313349928747655
1201	21	0.883802735382659
1201	22	0.9181400734265139
1201	23	0.9202616336869056
1201	24	0.9071927455084818
1201	25	0.7727181070613014
1202	1	0.3508367543400972
1202	2	0.3463764710639943
1202	3	0.35159029525826924
1202	4	0.4488850943289293
1202	5	0.4648518160784789
1202	6	0.42736533692544043
1202	7	0.4152956741326932
1202	8	0.37674673982315454
1202	9	0.47008915078843216
1202	10	0.5222539018647652
1202	11	0.43559320243493715
1202	12	0.4524531877407867
1202	13	0.3776500588062177
1202	14	0.5307401745605773
1202	15	0.45850194885267115
1202	16	0.4301363321731344
1202	17	0.4455426314496975
1202	18	0.5048614708427905
1202	19	0.4772281712511983
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2210	11	0.0019626386522091366
2210	12	0.09340919040176487
2210	13	0.09619806477774517
2210	14	0.029432623058276874
2210	15	0.017771471573233555
2210	16	0.07906241936131408
2210	17	0.01144100233270251
2210	18	0.02598045800399973
2210	19	0.013197943460312445
2210	20	-0.012024637394191018
2210	21	0.0046630180030504675
2210	22	-0.0022481700191576692
2210	23	8.849372429864388E-5
2210	24	0.11234202467893795
2210	25	0.03448946738065928
2211	1	-0.14413713550174734
2211	2	-0.1575271549383217
2211	3	-0.1388309107520449
2211	4	-0.19031760539767362
2211	5	-0.2263630412973754
2211	6	-0.1548749137468448
2211	7	-0.08783402538953311
2211	8	-0.17508433141864543
2211	9	-0.12950857557687812
2211	10	-0.20298911351512317
2211	11	-0.01997839884234054
2211	12	-0.04044798363018742
2211	13	0.016121478916176102
2211	14	-0.02107523358242247
2211	15	0.02591057233795624
2211	16	-0.0913330988496952
2211	17	-0.22092725410639957
2211	18	-0.13752937742727767
2211	19	-0.14746870385693
2211	20	-0.13556122444673946
2211	21	-0.17106738879186167
2211	22	-0.14323004304687714
2211	23	-0.06202860323274706
2211	24	-0.18613103079432136
2211	25	-0.1660289748741235
2212	1	-0.29247555082308097
2212	2	-0.23373852096825232
2212	3	-0.3168428108980308
2212	4	-0.21110070958189908
2212	5	-0.2333472167916355
2212	6	-0.26498898915802016
2212	7	-0.3471114964866189
2212	8	-0.3693459954425151
2212	9	-0.31570221763740847
2212	10	-0.38961206541539894
2212	11	-0.2414432371100972
2212	12	-0.301034664177493
2212	13	-0.29886525125933616
2212	14	-0.26576107302428653
2212	15	-0.31998752178375156
2212	16	-0.22808464281934704
2212	17	-0.2768116555288387
2212	18	-0.42748522276215084
2212	19	-0.40582766503675316
2212	20	-0.3175857024279054
2212	21	-0.29180266931037835
2212	22	-0.29527879308756866
2212	23	-0.31574292155518435
2212	24	-0.454119313962174
2212	25	-0.4288267253709179
2213	1	-0.4773280615262614
2213	2	-0.4881747482480492
2213	3	-0.5275486102120546
2213	4	-0.49743084962872075
2213	5	-0.5210016687740051
2213	6	-0.3913840466141991
2213	7	-0.47946116815840867
2213	8	-0.5153984774489189
2213	9	-0.5127871806548114
2213	10	-0.4804440890062338
2213	11	-0.3260432577283652
2213	12	-0.4695348698578101
2213	13	-0.3728544150785993
2213	14	-0.5811647500296289
2213	15	-0.5666842611160945
2213	16	-0.44155915503949217
2213	17	-0.5510050661626025
2213	18	-0.48039543675743346
2213	19	-0.48806055342941335
2213	20	-0.5565213675952236
2213	21	-0.5112434178058578
2213	22	-0.5196833500871421
2213	23	-0.4823740521833173
2213	24	-0.5506838264080613
2213	25	-0.47740258094709986
2214	1	-0.37531005943950646
2214	2	-0.3918987740796567
2214	3	-0.4759510396016964
2214	4	-0.5939115072179533
2214	5	-0.4948615801485019
2214	6	-0.4639858424857337
2214	7	-0.47140015051758155
2214	8	-0.4579145211692648
2214	9	-0.46906043425376964
2214	10	-0.4720358044780468
2214	11	-0.34226371650903076
2214	12	-0.3315125464028057
2214	13	-0.434724698131177
2214	14	-0.5066815226851489
2214	15	-0.4089905942764318
2214	16	-0.5151030466464803
2214	17	-0.5285534191091728
2214	18	-0.5301007823313526
2214	19	-0.5193961410896577
2214	20	-0.5456751670035018
2214	21	-0.5788473009258581
2214	22	-0.5325180799301563
2214	23	-0.5110166211105707
2214	24	-0.5791360540467778
2214	25	-0.5260902265554748
2215	1	-0.5259423219793611
2215	2	-0.46207014319297457
2215	3	-0.5835293753878119
2215	4	-0.5803871825000257
2215	5	-0.590456820981224
2215	6	-0.5496693452144541
2215	7	-0.4680248899092945
2215	8	-0.5851284460834165
2215	9	-0.6446184425932344
2215	10	-0.5379206462734842
2215	11	-0.5139762195750066
2215	12	-0.5887175991376594
2215	13	-0.5556062248358558
2215	14	-0.6580312245186022
2215	15	-0.6528283579507033
2215	16	-0.7135322110136642
2215	17	-0.6599202137804951
2215	18	-0.623034465186393
2215	19	-0.7351865123798191
2215	20	-0.7198341303956965
2215	21	-0.6514330333884502
2215	22	-0.7666291182242446
2215	23	-0.615054037562615
2215	24	-0.7267613568122968
2215	25	-0.6354550704970947
2216	1	-0.6164417907144966
2216	2	-0.7261390887779555
2216	3	-0.716949685490814
2216	4	-0.6287023124323241
2216	5	-0.677933441902816
2216	6	-0.5423641505448416
2216	7	-0.5907811080718162
2216	8	-0.5383704458742855
2216	9	-0.5825216727103495
2216	10	-0.6621479277474833
2216	11	-0.5255488738247251
2216	12	-0.5840358536022521
2216	13	-0.5773402592450552
2216	14	-0.6541828636807665
2216	15	-0.6250507889706256
2216	16	-0.8311754433122971
2216	17	-0.8947178934033104
2216	18	-0.7801947396367765
2216	19	-0.745698536955814
2216	20	-0.6786082565522733
2216	21	-0.7488863737841598
2216	22	-0.7681227003675986
2216	23	-0.9283901366302771
2216	24	-1.0786275217464407
2216	25	-0.9580602736355459
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	233601.0
3	13.0
4	1896.0
5	10804.0
6	14689.0
7	14614.0
8	12511.0
9	11871.0
10	12118.0
11	13856.0
12	22585.0
13	19865.0
14	9275.0
15	9241.0
16	9769.0
17	10452.0
18	11878.0
19	13967.0
20	17727.0
21	22313.0
22	27512.0
23	33110.0
24	39750.0
25	45692.0
26	52075.0
27	58673.0
28	67452.0
29	82694.0
30	104627.0
31	134223.0
32	173367.0
33	229333.0
34	316420.0
35	445906.0
36	665873.0
37	1227037.0
38	4053909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9743637789213	17.742475559347458	12.762323019111088	25.520837642620158
2	16.871101548519235	20.594752108533253	37.34653084595066	25.18761549699685
3	18.300662981771197	24.444432814110602	28.57211537934897	28.682788824769233
4	12.74547996449126	16.42117249932922	37.319723273368105	33.51362426281141
5	15.647870510254425	35.1709533267674	32.841533866081605	16.33964229689657
6	32.76253562039859	36.870294940183015	16.19251829986959	14.1746511395488
7	29.38788356428913	30.72301071488529	21.23560651896242	18.65349920186316
8	27.181255743425886	31.780166305421208	19.924787030516153	21.11379092063675
9	27.486796323140272	14.937197050371353	18.89937607376187	38.67663055272651
10	16.71093409004974	25.603300178358445	30.099063709345035	27.586702022246783
11	36.52655672669047	22.235318375410777	22.203869660372234	19.034255237526516
12	25.534394534704486	23.83451364648143	27.65319709561992	22.97789472319416
13	29.26073309495194	20.14661996889805	24.731478453083007	25.861168483067004
14	24.29954724432563	20.446512793014236	24.62953685257015	30.624403110089983
15	24.996507881912343	26.733038519469137	22.710069556142617	25.5603840424759
16	25.546022914302007	26.323997999282945	23.131657971327215	24.998321115087833
17	24.982851822623754	25.36024626837558	24.20079953071236	25.456102378288303
18	26.801534557639123	23.75252970569296	25.11572057957172	24.33021515709619
19	27.359757510964545	24.526437531000713	24.360496941059044	23.753308016975698
20	26.31630647128407	24.3873008649827	24.10582367858021	25.19056898515303
21	26.84184679739841	24.549807204821562	24.135522320513957	24.47282367726607
22	25.671079863200692	24.27820751809068	24.956981119151415	25.093731499557215
23	24.42264789109969	23.623746228013513	25.30203409041712	26.65157179046968
24	24.93380259725964	25.371110914055595	24.64206802288426	25.05301846580051
25	24.941334456887386	24.517836930153543	24.869334862932316	25.67149375002675
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4086.0
1	4086.0
2	4336.0
3	4586.0
4	4586.0
5	4586.0
6	8108.5
7	11631.0
8	11631.0
9	11631.0
10	16130.5
11	20630.0
12	20630.0
13	20630.0
14	27871.5
15	35113.0
16	35113.0
17	35113.0
18	57070.0
19	79027.0
20	79027.0
21	79027.0
22	113764.5
23	148502.0
24	148502.0
25	148502.0
26	206609.0
27	264716.0
28	264716.0
29	264716.0
30	316677.0
31	368638.0
32	368638.0
33	368638.0
34	458708.5
35	548779.0
36	548779.0
37	548779.0
38	610537.0
39	672295.0
40	672295.0
41	672295.0
42	763217.0
43	854139.0
44	854139.0
45	854139.0
46	916136.5
47	978134.0
48	978134.0
49	978134.0
50	999687.0
51	1021240.0
52	1021240.0
53	1021240.0
54	951603.5
55	881967.0
56	881967.0
57	881967.0
58	827513.5
59	773060.0
60	773060.0
61	773060.0
62	685733.5
63	598407.0
64	598407.0
65	598407.0
66	502656.0
67	406905.0
68	406905.0
69	406905.0
70	316176.5
71	225448.0
72	225448.0
73	225448.0
74	186100.5
75	146753.0
76	146753.0
77	146753.0
78	124400.0
79	102047.0
80	102047.0
81	102047.0
82	76012.0
83	49977.0
84	49977.0
85	49977.0
86	37068.0
87	24159.0
88	24159.0
89	24159.0
90	16858.5
91	9558.0
92	9558.0
93	9558.0
94	5128.0
95	698.0
96	698.0
97	698.0
98	450.5
99	203.0
100	203.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.14596574919891364
2	0.16393506358755966
3	0.1778099500188198
4	0.1990353673528053
5	0.18906051467323912
6	0.07017630825477984
7	0.05667806059704778
8	0.06061454326230898
9	0.06715104842869948
10	0.06910714012347434
11	0.7127342055315358
12	0.6704656154314008
13	0.6682786806173668
14	0.6680599871359635
15	0.6699796299171711
16	0.6758722042772072
17	0.667039417556081
18	0.6783628800376348
19	0.6740983571502684
20	0.6799909315103045
21	0.6672945599510516
22	0.6708787031184962
23	0.6557402543502386
24	0.6654721142726898
25	0.6592757989662602
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	8230698.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.49863859433487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.70029542146553	33.9045103274579
2	11.339842684084617	9.411760665268796
3	2.7910372922423665	3.474727436822309
4	1.1821748344935137	1.9623458484785588
5	0.6170638944212452	1.280365577210003
6	0.4133463747133356	1.0291987091104338
7	0.2732124565421621	0.7936561495459524
8	0.20682586244183537	0.6866393373948275
9	0.15444627814883377	0.5768379251224715
>10	1.0612804285286417	8.638545157111297
>50	0.12362339639029812	3.552345115329355
>100	0.1017918428046967	8.617887708948437
>500	0.016045651301758324	4.644438246491045
>1k	0.016780147701441362	14.371378119785778
>5k	0.0018513998335531862	5.130343191452397
>10k+	3.8203488628875274E-4	1.925020484470583
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACAAAGGGCAGGGACTTAATCAA	18535	0.22519353765622308	No Hit
GAATAGGACCGCGGTTCTATTTTGT	13418	0.1630238407483788	No Hit
GTATCTGATCGTCTTCGAACCTCCG	13321	0.16184532587637163	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	12389	0.15052186339481777	No Hit
CTATTGGAGCTGGAATTACCGCGGC	12309	0.14954989236635824	No Hit
GTCCTATTCCATTATTCCTAGCTGC	12257	0.14891811119785953	No Hit
GTATCAACGCAGAGTACTTTTTTTT	11983	0.14558911042538555	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	11980	0.1455526615118183	No Hit
GAACTACGACGGTATCTGATCGTCT	10423	0.12663567537042425	No Hit
CTTTAATATACGCTATTGGAGCTGG	10375	0.1260524927533485	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	10323	0.1254207115848498	No Hit
GTATCAACGCAGAGTACGGGCGGTG	10276	0.12484967860562979	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	10260	0.12465528439993791	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	9923	0.12056085644255202	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	9856	0.11974683070621714	No Hit
ATCAGATACCGTCGTAGTTCCGACC	9581	0.11640568029588742	No Hit
TCGTAGTTCCGACCATAAACGATGC	9274	0.11267574147417388	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	9122	0.11082899652010071	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	9056	0.11002712042162159	No Hit
CTTATGACCCGCACTTACTGGGAAT	8979	0.10909159830672928	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	8912	0.1082775725703944	No Hit
GACTTAATCAACGCAAGCTTATGAC	8843	0.10743924755834804	No Hit
GCTTTGAACACTCTAATTTTTTCAA	8832	0.10730560154193484	No Hit
GAATAACGCCGCCGCATCGCCAGTC	8489	0.10313827575741451	No Hit
GAATAATTGCAATCCCCGATCCCCA	8409	0.10216630472895494	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.859855142297773E-5
2	0.0	0.0	0.0	0.0	4.859855142297773E-5
3	0.0	0.0	0.0	0.0	4.859855142297773E-5
4	0.0	0.0	0.0	1.2149637855744433E-5	4.859855142297773E-5
5	0.0	2.4299275711488866E-5	0.0	2.4299275711488866E-5	4.859855142297773E-5
6	0.0	2.4299275711488866E-5	0.0	2.4299275711488866E-5	7.289782713446661E-5
7	0.0	2.4299275711488866E-5	0.0	2.4299275711488866E-5	7.289782713446661E-5
8	0.0	2.4299275711488866E-5	0.0	2.4299275711488866E-5	7.289782713446661E-5
9	0.0	2.4299275711488866E-5	0.0	7.289782713446661E-5	7.289782713446661E-5
10	0.0	2.4299275711488866E-5	0.0	7.289782713446661E-5	7.289782713446661E-5
11	0.0	2.4299275711488866E-5	0.0	7.289782713446661E-5	7.289782713446661E-5
12	0.0	2.4299275711488866E-5	0.0	7.289782713446661E-5	1.3364601641318877E-4
13	0.0	2.4299275711488866E-5	0.0	8.504746499021104E-5	1.7009492998042207E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4905	0.0	15.994877	1
GTATCAA	17205	0.0	13.746021	1
ACCGTCC	500	0.0	11.983499	8
AGGCCCG	2330	0.0	11.674167	10
AACCGCG	1045	0.0	11.648846	7
CGAACGA	830	0.0	11.456939	16
CGGTCCA	2090	0.0	11.285509	10
ACGCTTA	280	0.0	11.206861	17
TAGATCG	255	0.0	11.188527	5
CGTCGTA	1780	0.0	11.11371	10
CGAGCCG	2450	0.0	10.945351	15
CGGTTCT	2555	0.0	10.941759	12
ACTCTAA	1820	0.0	10.921711	10
TCCATTA	2320	0.0	10.658558	8
CGCCAGT	1580	0.0	10.652255	18
CCGTCGT	1840	0.0	10.647864	9
CTCGACG	295	0.0	10.637736	13
CCGTCCA	430	0.0	10.616611	9
GTATTAG	1560	0.0	10.616439	1
ATACCGT	1920	0.0	10.600029	6
>>END_MODULE