FastQCFastQC Report
Thu 2 Feb 2017
SRR4050275_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4050275_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8230698
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT192320.23366183524167694No Hit
GTACAAAGGGCAGGGACTTAATCAA181050.21996919337825296No Hit
GAATAGGACCGCGGTTCTATTTTGT140240.17038652128895992No Hit
GATTAAGAGGGACGGCCGGGGGCAT128960.15668172978768022No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG122600.14895456011142677No Hit
CTATTGGAGCTGGAATTACCGCGGC122460.14878446518144633No Hit
GTATCTGATCGTCTTCGAACCTCCG122060.14829847966721657No Hit
ATACAGGACTCTTTCGAGGCCCTGT113070.1373759552349023No Hit
TATCAACGCAGAGTACTTTTTTTTT113050.13735165595919083No Hit
ATCAGATACCGTCGTAGTTCCGACC107960.1311674902906169No Hit
GTCCTATTCCATTATTCCTAGCTGC105470.12814223046453654No Hit
GAACTACGACGGTATCTGATCGTCT104610.12709736160894253No Hit
TCGTAGTTCCGACCATAAACGATGC103530.12578520072052213No Hit
CTTTAATATACGCTATTGGAGCTGG103440.12567585397982042No Hit
GTATCAACGCAGAGTACGGGCGGTG99140.12045150970185033No Hit
CTCTTAATCATGGCCTCAGTTCCGA99020.12030571404758139No Hit
ATATTAAAGTTGCTGCAGTTAAAAA97220.1181187792335474No Hit
CTTATGACCCGCACTTACTGGGAAT93670.11380565779475812No Hit
GACTTAATCAACGCAAGCTTATGAC89790.10909159830672928No Hit
GGGTAGGCACACGCTGAGCCAGTCA89590.10884860554961437No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC89570.10882430627390288No Hit
GAATAACGCCGCCGCATCGCCAGTC88890.10799813089971226No Hit
GTCTTGCGCCGGTCCAAGAATTTCA88080.10701401023339696No Hit
GCTTTGAACACTCTAATTTTTTCAA83390.10131583007905283No Hit
GAATAATGGAATAGGACCGCGGTTC83020.10086629347839028No Hit
GAATAATTGCAATCCCCGATCCCCA82950.10078124601340009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50750.016.4879681
GTATCAA172850.013.548151
CGTCGTA20400.012.148128510
CTCGACG2950.011.90482613
CCGTCGT20950.011.7840979
CGCCAGT16600.011.66412718
ATACCGT22150.011.322526
ATCGCCA17300.011.30203216
CGCTTAC3200.011.27103418
GTCGTAG21650.011.22744911
CGAACGA9700.011.15497416
ACCGTCG22650.011.151178
TACCGTC22350.011.132137
TCGCCAG17750.011.122313517
CGGTTCT24800.011.02568412
AACCGCG11400.010.9957177
TCGACGC3200.010.97456214
TAATACC5600.010.8769814
GCATCGC18000.010.80977914
CGGTCCA19650.010.77559810
Produced by FastQC (version 0.11.5)